##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033122_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1181887
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.46361454183014	33.0	31.0	34.0	30.0	34.0
2	31.649960613831947	33.0	31.0	34.0	30.0	34.0
3	31.865293382531494	34.0	31.0	34.0	30.0	34.0
4	35.20306171402173	37.0	35.0	37.0	33.0	37.0
5	35.34626152923249	37.0	35.0	37.0	33.0	37.0
6	35.397656459543086	37.0	37.0	37.0	35.0	37.0
7	35.31802025066694	37.0	36.0	37.0	33.0	37.0
8	35.343743522011835	37.0	36.0	37.0	33.0	37.0
9	37.00712335443236	39.0	38.0	39.0	34.0	39.0
10-11	36.99965013575748	39.0	38.0	39.0	34.5	39.0
12-13	36.96107199757675	39.0	38.0	39.0	34.0	39.0
14-15	38.426336866384005	41.0	39.0	41.0	34.0	41.0
16-17	38.443792426856376	41.0	39.0	41.0	34.5	41.0
18-19	38.42877872419275	41.0	39.0	41.0	34.0	41.0
20-21	38.46133767441388	41.0	39.0	41.0	34.0	41.0
22-23	38.43255150450085	41.0	39.0	41.0	34.0	41.0
24-25	38.43054581360147	41.0	39.0	41.0	34.0	41.0
26-27	38.39249818299042	41.0	39.0	41.0	34.0	41.0
28-29	38.37258849619295	40.5	39.0	41.0	34.0	41.0
30-31	38.37617090297127	41.0	39.0	41.0	34.0	41.0
32-33	38.43401695762793	41.0	39.0	41.0	34.0	41.0
34-35	38.41302214171067	41.0	39.0	41.0	34.0	41.0
36-37	38.332128198381064	41.0	39.0	41.0	34.0	41.0
38-39	38.242690714086876	41.0	39.0	41.0	33.0	41.0
40-41	38.17747762687973	41.0	38.5	41.0	33.0	41.0
42-43	38.07056046813274	40.5	38.0	41.0	33.0	41.0
44-45	37.94476460101515	40.0	38.0	41.0	33.0	41.0
46-47	37.81507284537354	40.0	38.0	41.0	33.0	41.0
48-49	37.697711794782414	40.0	38.0	41.0	32.5	41.0
50-51	37.58262041971864	40.0	38.0	41.0	32.0	41.0
52-53	37.41902821504932	40.0	37.0	41.0	32.0	41.0
54-55	37.26027530550721	40.0	37.0	41.0	31.0	41.0
56-57	37.104583602324084	40.0	36.5	41.0	31.0	41.0
58-59	36.9031819454821	40.0	36.0	41.0	31.0	41.0
60-61	36.67097912067736	39.0	35.5	41.0	31.0	41.0
62-63	36.37878325085224	39.0	35.0	41.0	31.0	41.0
64-65	36.08557120943034	39.0	35.0	41.0	30.0	41.0
66-67	35.78304947934955	38.0	35.0	40.0	30.0	41.0
68-69	35.45502615732299	37.0	35.0	40.0	30.0	41.0
70-71	35.08367170465535	37.0	35.0	40.0	29.5	41.0
72-73	34.70986397176718	36.0	34.0	39.0	29.0	41.0
74-75	34.34722016571804	36.0	34.0	39.0	29.0	41.0
76-77	34.023380830823925	35.0	34.0	38.5	29.0	40.0
78-79	33.66146848218146	35.0	34.0	37.0	28.0	39.0
80-81	33.31008463584082	35.0	34.0	37.0	28.0	39.0
82-83	33.103182030092555	35.0	34.0	36.5	28.0	39.0
84-85	32.83593059234936	35.0	34.0	36.0	28.0	37.0
86-87	32.593714965982365	35.0	34.0	36.0	27.0	37.0
88-89	32.24271440501503	35.0	34.0	35.0	27.0	37.0
90-91	32.05486776654621	35.0	34.0	35.0	26.5	36.0
92-93	31.87816051788369	35.0	33.5	35.0	26.0	36.0
94-95	31.702219839967782	35.0	33.0	35.0	25.5	36.0
96-97	31.515702431789165	35.0	33.0	35.0	25.0	36.0
98-99	31.31887058576666	35.0	33.0	35.0	25.0	35.0
100	31.15996453129614	35.0	33.0	35.0	24.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.2887368001750765
1101	2	0.338665758788526
1101	3	0.3935496723621057
1101	4	0.2789586143507705
1101	5	0.2016780768781672
1101	6	0.27967598460897136
1101	7	0.37655958527012245
1101	8	0.3342895302995359
1101	9	0.4045780041127003
1101	10-11	0.4751214148687666
1101	12-13	0.4900564826576712
1101	14-15	0.5080799207597337
1101	16-17	0.4035916109210902
1101	18-19	0.370887330412188
1101	20-21	0.4518394982329994
1101	22-23	0.41282085655804934
1101	24-25	0.4181312456268387
1101	26-27	0.4569691404812346
1101	28-29	0.5165471008563429
1101	30-31	0.495948920018364
1101	32-33	0.5460825287796709
1101	34-35	0.5336118488414314
1101	36-37	0.574812608744466
1101	38-39	0.5332600421040894
1101	40-41	0.5725983926172518
1101	42-43	0.4390061367575484
1101	44-45	0.38841952639106836
1101	46-47	0.5022427951204449
1101	48-49	0.3823913608336298
1101	50-51	0.42926417892113733
1101	52-53	0.5205299614513592
1101	54-55	0.5277150335652507
1101	56-57	0.5560170007661895
1101	58-59	0.5163236703734597
1101	60-61	0.5072718272891308
1101	62-63	0.5480339981246587
1101	64-65	0.5687252230117039
1101	66-67	0.5883831958555632
1101	68-69	0.5285214925264086
1101	70-71	0.509931014003925
1101	72-73	0.5388753670243744
1101	74-75	0.5703102161699931
1101	76-77	0.5005813080045556
1101	78-79	0.5158422272751508
1101	80-81	0.4889647008729341
1101	82-83	0.49608253247918555
1101	84-85	0.39848203061836784
1101	86-87	0.4106161949206424
1101	88-89	0.6797520947741873
1101	90-91	0.587924287665686
1101	92-93	0.5115227065872077
1101	94-95	0.6125951900120725
1101	96-97	0.5446461082766803
1101	98-99	0.7078767155200936
1101	100	0.7027564925534691
1102	1	0.05660806276409147
1102	2	0.017417879626947297
1102	3	0.06629130688875051
1102	4	0.11267722493460752
1102	5	0.03256715652496922
1102	6	0.06418388964377186
1102	7	0.035624683423129966
1102	8	0.16581657426386442
1102	9	0.263346890153656
1102	10-11	0.15998034133158967
1102	12-13	0.2102414237981023
1102	14-15	0.09312181361558203
1102	16-17	0.10024038395813051
1102	18-19	0.11348484867860975
1102	20-21	0.16198647586193715
1102	22-23	0.14747586012467906
1102	24-25	0.10988123454407628
1102	26-27	0.15129456273462694
1102	28-29	0.11329843985599553
1102	30-31	0.09138261888148236
1102	32-33	0.11244492314075671
1102	34-35	0.14565438248266815
1102	36-37	0.13283955397142222
1102	38-39	0.22488368748781795
1102	40-41	0.1724714445692257
1102	42-43	0.16897716036182686
1102	44-45	0.19938421065313605
1102	46-47	0.243782211481836
1102	48-49	0.19813956844809155
1102	50-51	0.25475736201246235
1102	52-53	0.20368133591586002
1102	54-55	0.19181976771992026
1102	56-57	0.12773096061836497
1102	58-59	0.1501919990520335
1102	60-61	0.13981909024892047
1102	62-63	0.14011022337482615
1102	64-65	0.20994283614660247
1102	66-67	0.13803680903880888
1102	68-69	0.07142187256452814
1102	70-71	0.14545047332613592
1102	72-73	0.08736549127225146
1102	74-75	0.12979968351210402
1102	76-77	0.15220704903860138
1102	78-79	0.014770826050806818
1102	80-81	0.12625438019814084
1102	82-83	0.12364738681133502
1102	84-85	0.12631629691910717
1102	86-87	0.1800678599551162
1102	88-89	0.3225952474382865
1102	90-91	0.26819918056586545
1102	92-93	0.29820525183512103
1102	94-95	0.3997625559005229
1102	96-97	0.47215615329285754
1102	98-99	0.6410915640086117
1102	100	0.5647998322140566
1103	1	-0.15369449524183665
1103	2	-0.2289758648151583
1103	3	-0.1264501071722215
1103	4	-0.1611676337408241
1103	5	-0.08596269635793163
1103	6	-0.1712239483852116
1103	7	-0.10367448628201004
1103	8	-0.06236718654144369
1103	9	-0.16441594016774985
1103	10-11	-0.07467626850946374
1103	12-13	-0.08360483797894602
1103	14-15	-0.007664871329325251
1103	16-17	-0.03470063837204407
1103	18-19	-0.05309118520819567
1103	20-21	-0.1546241615443762
1103	22-23	-0.10216037630173247
1103	24-25	-0.13494862042451672
1103	26-27	-0.08258692826940717
1103	28-29	-0.11243633980964773
1103	30-31	-0.08043475796317523
1103	32-33	-0.10300265679336462
1103	34-35	-0.04963972847232867
1103	36-37	-0.15657556122511807
1103	38-39	-0.157225250079847
1103	40-41	-0.12547111548446566
1103	42-43	-0.0707833264006652
1103	44-45	-0.16473317206937566
1103	46-47	-0.16658784708817365
1103	48-49	-0.03697627275872861
1103	50-51	-0.15759732690663242
1103	52-53	-0.16677806609740742
1103	54-55	-0.216694783771068
1103	56-57	-0.35889899787637347
1103	58-59	-0.27435697014229987
1103	60-61	-0.39066063777689664
1103	62-63	-0.258243407529811
1103	64-65	-0.3161178802315803
1103	66-67	-0.20941513947115453
1103	68-69	-0.11869839599670229
1103	70-71	-0.20246108576624522
1103	72-73	-0.0915166918021697
1103	74-75	-0.12471214141115183
1103	76-77	-0.16637399809233955
1103	78-79	-0.1327006521167391
1103	80-81	-0.18974988978136054
1103	82-83	-0.13867061289666793
1103	84-85	-0.123724374066839
1103	86-87	-0.13278227552003585
1103	88-89	-0.020031927606225963
1103	90-91	0.012046105972054022
1103	92-93	0.02123697520415746
1103	94-95	-0.07597933407734558
1103	96-97	-0.005614356165750678
1103	98-99	0.10864787294124767
1103	100	0.11208644226367426
1104	1	0.06421087208531873
1104	2	-0.007497539258928043
1104	3	0.09580592853481917
1104	4	0.09805781203361619
1104	5	0.11465270906575142
1104	6	0.17344817352367414
1104	7	0.01591253835674422
1104	8	0.1419451598889765
1104	9	0.12866807507086264
1104	10-11	0.07473988531935305
1104	12-13	0.10352462258326511
1104	14-15	0.04490946198146162
1104	16-17	0.07794335071746872
1104	18-19	-0.0019764673253774845
1104	20-21	0.0734308868089073
1104	22-23	-0.0069134251509481714
1104	24-25	-0.003865010947720293
1104	26-27	0.0715856557571044
1104	28-29	0.09751722300453025
1104	30-31	0.16752516178615906
1104	32-33	0.09552758373322234
1104	34-35	0.0611670612754196
1104	36-37	0.028942123227444938
1104	38-39	-0.06302651695841632
1104	40-41	-0.03731372498380381
1104	42-43	-0.032838960854356
1104	44-45	-0.04712423629244711
1104	46-47	-0.017385845329876304
1104	48-49	-0.1569408813112787
1104	50-51	-0.09036484792735422
1104	52-53	-0.11149301842038994
1104	54-55	-0.1653151935905015
1104	56-57	-0.1291589108630049
1104	58-59	-0.07127318032692642
1104	60-61	-0.13446713447444836
1104	62-63	-0.08924361041071904
1104	64-65	-0.06107403541293621
1104	66-67	-0.12088580063748822
1104	68-69	-0.1742764938754675
1104	70-71	-0.0796279665849795
1104	72-73	-0.10285548037771974
1104	74-75	-0.14272345765740369
1104	76-77	-0.14994628005172217
1104	78-79	-0.014184387830546541
1104	80-81	0.026470438885702663
1104	82-83	-0.05827905274247769
1104	84-85	-0.1690738927882336
1104	86-87	-0.026754458293190453
1104	88-89	0.19388344851243744
1104	90-91	0.1549531639734525
1104	92-93	0.09238618284149425
1104	94-95	0.0860492495884877
1104	96-97	0.22449204359980968
1104	98-99	0.2728348840546104
1104	100	0.3771503497499822
1105	1	-0.12155934652593814
1105	2	-0.014376051772885035
1105	3	-0.023889442812322415
1105	4	-0.07719414304732908
1105	5	-0.12358750216318981
1105	6	-0.1797536763746379
1105	7	-0.23861298822474453
1105	8	-0.11985267147744594
1105	9	-0.20360931919643122
1105	10-11	-0.20455496947103313
1105	12-13	-0.19726738312517966
1105	14-15	-0.27515985779636765
1105	16-17	-0.17982299371812616
1105	18-19	-0.10772254431124395
1105	20-21	-0.1544129000127228
1105	22-23	-0.07133958858604927
1105	24-25	-0.14216835232141278
1105	26-27	-0.10881181739202361
1105	28-29	-0.046962899225292176
1105	30-31	-0.1725730703641517
1105	32-33	-0.25395740177974346
1105	34-35	-0.1970671042653862
1105	36-37	-0.08422791483571501
1105	38-39	-0.1474188738925477
1105	40-41	-0.18516524370590304
1105	42-43	-0.23560136736724502
1105	44-45	-0.2239148601293195
1105	46-47	-0.15833520343819174
1105	48-49	-0.17667933332722896
1105	50-51	-0.17437009574916118
1105	52-53	-0.13694043018726632
1105	54-55	-0.1020344207294741
1105	56-57	-0.02807356589891441
1105	58-59	-0.12345425485545292
1105	60-61	-0.07808697995982072
1105	62-63	-0.04914774307039238
1105	64-65	-0.024919175317144493
1105	66-67	-0.04146571373586738
1105	68-69	-0.16185301604195956
1105	70-71	-0.18418958222315496
1105	72-73	-0.1414697428551719
1105	74-75	-0.21552875976701813
1105	76-77	-0.1969664138382754
1105	78-79	-0.1544443524200858
1105	80-81	-0.15003743593153018
1105	82-83	-0.12740810350774723
1105	84-85	-0.18232762456121065
1105	86-87	-0.1824915901282651
1105	88-89	-0.1090898373601874
1105	90-91	-0.19660154436322586
1105	92-93	-0.13643745785544326
1105	94-95	-0.08377855111766763
1105	96-97	-0.07814759842965202
1105	98-99	-0.01233549536372891
1105	100	-0.005051267780046942
1106	1	0.014440388528758064
1106	2	-0.09200989541814408
1106	3	-0.035773018833602066
1106	4	-0.054900749244126246
1106	5	0.057399360640928876
1106	6	0.02963882937476825
1106	7	-0.013264346507746438
1106	8	0.009548756694613303
1106	9	0.02941007343594748
1106	10-11	0.029166723022932217
1106	12-13	-0.06067395630122263
1106	14-15	0.0014113798585952964
1106	16-17	-0.07218555577815522
1106	18-19	0.00534919449944482
1106	20-21	0.028203571208081257
1106	22-23	0.10738890774221943
1106	24-25	0.12070268068278978
1106	26-27	0.14567545013552774
1106	28-29	0.23014943076407235
1106	30-31	0.14358313826858193
1106	32-33	0.14903949972391217
1106	34-35	0.12577751213375166
1106	36-37	0.11491646769075459
1106	38-39	0.20398285234298186
1106	40-41	0.21623906379819147
1106	42-43	0.17232673115344
1106	44-45	0.17143515501737028
1106	46-47	0.14781883303269439
1106	48-49	0.15641194519944435
1106	50-51	0.2120623670635311
1106	52-53	0.12110460466959694
1106	54-55	0.04308888111238218
1106	56-57	0.06941997362419983
1106	58-59	0.09143196964478051
1106	60-61	0.04820280201552407
1106	62-63	0.14788146033064464
1106	64-65	0.08188641230133697
1106	66-67	0.21661341334728945
1106	68-69	0.12060497502976375
1106	70-71	0.14671297537565664
1106	72-73	0.1459340561877127
1106	74-75	0.009354766577018836
1106	76-77	0.022646941353045236
1106	78-79	0.1754968043973406
1106	80-81	0.18988300255293922
1106	82-83	0.019960901983047563
1106	84-85	-0.019683840596826485
1106	86-87	0.12441198828921785
1106	88-89	0.13043630017564567
1106	90-91	0.10480539311260628
1106	92-93	0.09957036192720636
1106	94-95	0.11187770962747123
1106	96-97	0.16517933530577622
1106	98-99	0.17282230781113483
1106	100	0.21614299264754777
1107	1	-0.1622507720883064
1107	2	0.02112183655670208
1107	3	-0.07439454223565889
1107	4	-0.041803444470403406
1107	5	-0.0711271583359121
1107	6	-0.06917679210308592
1107	7	-0.061336311778624975
1107	8	-0.10015235723712834
1107	9	-0.07400560810638979
1107	10-11	-0.02551886363600886
1107	12-13	-0.13430405784076527
1107	14-15	-0.1422381072843919
1107	16-17	-0.07028346087819415
1107	18-19	-0.13463766520159481
1107	20-21	-0.06115571660427577
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2216	22-23	-0.41084559053502545
2216	24-25	-0.4337389150344464
2216	26-27	-0.5157988780295284
2216	28-29	-0.4534677263067337
2216	30-31	-0.4676463519358194
2216	32-33	-0.4329582001412575
2216	34-35	-0.44861874148654124
2216	36-37	-0.49981159936374553
2216	38-39	-0.4821916572528835
2216	40-41	-0.5151217556781589
2216	42-43	-0.49769780985990764
2216	44-45	-0.5360769802484526
2216	46-47	-0.3665953712101171
2216	48-49	-0.4380667949383792
2216	50-51	-0.4345238937141218
2216	52-53	-0.39519152806617797
2216	54-55	-0.5064091412502094
2216	56-57	-0.5456539979211286
2216	58-59	-0.5490858779866343
2216	60-61	-0.4454429584958106
2216	62-63	-0.40125193188447383
2216	64-65	-0.48887004180831894
2216	66-67	-0.546900398905116
2216	68-69	-0.5480984969150384
2216	70-71	-0.6339887790103091
2216	72-73	-0.5311505775384475
2216	74-75	-0.4951825640598244
2216	76-77	-0.5330648780733611
2216	78-79	-0.5167852601687315
2216	80-81	-0.5274239515317518
2216	82-83	-0.5264459566007957
2216	84-85	-0.5675135890072269
2216	86-87	-0.6090042320826328
2216	88-89	-0.6000502652060256
2216	90-91	-0.5747559687835953
2216	92-93	-0.6476166283188078
2216	94-95	-0.6580593929724827
2216	96-97	-0.61893126882552
2216	98-99	-0.6429598836732708
2216	100	-0.6005981085042897
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	11496.0
3	1986.0
4	1300.0
5	1247.0
6	982.0
7	998.0
8	1222.0
9	1361.0
10	1485.0
11	1673.0
12	1956.0
13	2291.0
14	2458.0
15	2649.0
16	2625.0
17	2646.0
18	2547.0
19	2485.0
20	2521.0
21	2750.0
22	3004.0
23	4101.0
24	6520.0
25	9222.0
26	9213.0
27	7677.0
28	8169.0
29	10303.0
30	12326.0
31	14955.0
32	19489.0
33	27006.0
34	37942.0
35	57522.0
36	109067.0
37	243088.0
38	385401.0
39	167706.0
40	498.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.46398844112196	19.435061533877377	12.539888570921645	23.561061454079013
2	16.520768283323537	19.950665056052426	39.8744771292213	23.654089531402743
3	18.516292186675855	25.083372207909417	29.480700572070077	26.91963503334465
4	12.515891812098262	16.925901410668125	38.836456959640294	31.721749817593313
5	12.403970191399386	37.211064123703665	34.88603390542101	15.498931779475948
6	30.200061093816238	38.292960926189025	18.2276193127538	13.279358667240936
7	27.23167331604354	31.984870728498116	22.504114434154246	18.279341521304097
8	24.8267574943409	35.83168672914595	21.054052338742093	18.287503437771054
9	24.32704602633679	17.576646387852005	21.572608113951762	36.523699471859445
10-11	23.614710692396063	26.8908715088763	28.680899499220374	20.813518299507262
12-13	24.64593861807744	24.09508714445772	28.673767639602072	22.58520659786277
14-15	22.09795654133143	26.30021642594657	25.832710994141898	25.76911603858009
16-17	21.568448244127524	29.71301391454098	25.8497771176992	22.868760723632295
18-19	21.377569694059105	28.361806153514163	28.076111815144934	22.1845123372818
20-21	23.017412461652388	28.05653231778271	27.109404421876654	21.816650798688247
22-23	22.30139119249907	28.344954811630508	27.263903462749212	22.089750533121215
24-25	22.094713390754624	28.003097449047598	27.588411647593237	22.313777512604542
26-27	21.99469228451792	27.606298173103287	27.726254912514094	22.6727546298647
28-29	22.206279780126877	27.789510117573986	27.51418212098607	22.49002798131307
30-31	22.548413535606645	28.841295168653474	26.79512062816264	21.815170667577245
32-33	21.701184658845673	28.03349847580327	27.898639648072198	22.366677217278855
34-35	21.629452612374145	27.30942641331887	28.18217821111903	22.878942763187958
36-37	22.728774685530134	28.249846313707888	26.95080050144251	22.070578499319467
38-39	22.48240111495569	28.324706304458385	27.32407249919822	21.868820081387703
40-41	21.784924927567626	27.89183714223823	27.86463269340121	22.458605236792938
42-43	21.58034264478041	28.027207488193927	27.724789242236675	22.667660624788986
44-45	22.231022331546658	28.054198519466222	27.604208313818667	22.110570835168453
46-47	21.960119663854737	28.79116406564335	27.190738278260344	22.057977992241565
48-49	21.75217933015609	28.067614762542387	27.85434607403271	22.325859833268815
50-51	21.812837242675958	27.66255128711732	27.966064407057882	22.558547063148836
52-53	21.924817399662892	27.75377046849933	27.6743587834316	22.647053348406182
54-55	22.098447820776585	28.75036647912243	27.30073795039914	21.850447749701846
56-57	21.521179768483382	28.254174144293014	27.452301839728765	22.772344247494836
58-59	21.29852482090677	27.518611608092865	28.217808168758218	22.96505540224215
60-61	22.154837170262166	28.33410049346447	27.32039145473868	22.190670881534682
62-63	21.957888413179226	28.203975090112195	27.314445027329803	22.523691469378775
64-65	21.715650625514808	27.72724330539871	27.900443916466035	22.656662152620452
66-67	21.610516901619384	27.953309546151058	27.40654844149394	23.029625110735623
68-69	22.466644639175737	28.160906167542336	27.353278507162248	22.019170686119676
70-71	22.544001374097668	27.699998265455534	27.606205946272272	22.14979441417453
72-73	21.89759529288132	28.031732775911383	28.062235297551887	22.008436633655407
74-75	21.851978926536056	27.95779215147578	27.807901999000734	22.38232692298743
76-77	22.07380512792742	28.06978428588593	27.388517246806256	22.46789333938039
78-79	22.344393784847274	28.4004320332427	27.39493515905313	21.860239022856895
80-81	22.08232855269281	27.795786267292804	27.84617337225536	22.27571180775902
82-83	21.74019544591308	28.075920909514355	27.852248338743586	22.331635305828982
84-85	21.911711449487754	28.034178560428884	27.591018164710977	22.46309182537238
86-87	22.311144805517046	28.190541301477456	27.462792900539785	22.035520992465717
88-89	21.772065805529532	28.018253871307824	27.659671363920175	22.550008959242472
90-91	21.614395663661327	27.783181363687248	27.787608283959692	22.814814688691733
92-93	22.112494705993775	28.24375200853	27.384324633782462	22.259428651693764
94-95	21.891450539376596	27.883048461967004	27.632799170261872	22.592701828394528
96-97	21.76853985662213	27.816243108265397	27.824839822362947	22.590377212749523
98-99	21.716534434485595	27.938263182824276	27.740287286347108	22.60491509634302
100	22.211440988491603	27.937637939501958	27.452312191617708	22.398608880388736
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	317.0
1	275.0
2	216.0
3	177.0
4	147.0
5	112.5
6	94.5
7	102.5
8	104.5
9	111.0
10	111.5
11	102.5
12	106.0
13	127.0
14	136.5
15	158.0
16	204.0
17	293.0
18	410.0
19	585.5
20	873.5
21	1193.5
22	1782.5
23	2763.5
24	3981.0
25	5394.5
26	7327.5
27	9751.5
28	12609.5
29	15832.0
30	19105.0
31	22293.5
32	25323.0
33	28557.5
34	31429.5
35	33672.5
36	35493.5
37	37349.5
38	38763.0
39	39342.5
40	39614.5
41	39748.0
42	39790.0
43	39672.0
44	38991.5
45	38630.0
46	38763.5
47	38282.0
48	38430.5
49	38899.5
50	38803.5
51	37778.0
52	35077.5
53	32958.5
54	32298.5
55	31463.0
56	29635.5
57	27668.0
58	25442.5
59	22547.0
60	19813.5
61	16954.0
62	14047.0
63	11342.0
64	9110.0
65	7195.5
66	5504.0
67	4089.0
68	3093.5
69	2426.5
70	1848.5
71	1397.5
72	1069.5
73	827.0
74	633.0
75	454.0
76	289.5
77	197.5
78	153.5
79	107.5
80	74.5
81	47.5
82	41.0
83	30.0
84	13.5
85	10.5
86	12.0
87	9.0
88	3.5
89	6.0
90	4.5
91	3.0
92	3.5
93	2.5
94	1.5
95	1.5
96	1.5
97	1.0
98	0.5
99	1.0
100	1.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3315037732033604
2	0.11718548389143801
3	0.06244251777031138
4	0.039005420992023775
5	0.040866851061057446
6	0.008291824852968177
7	0.0060919529532011095
8	0.013706894144702497
9	0.016414428790569657
10-11	0.019418100038328535
12-13	0.014468388263852636
14-15	0.016033681730994585
16-17	0.017725890884661564
18-19	0.01730283859624482
20-21	0.02216793991303737
22-23	0.01412994643311924
24-25	0.022802518345662486
26-27	0.017852806571186586
28-29	0.017387449053928167
30-31	0.018487385003811703
32-33	0.023775538609021
34-35	0.013960725517752544
36-37	0.008164909166443154
38-39	0.01150702224493543
40-41	0.008037993479918131
42-43	0.006768836614667899
44-45	0.00706497321655962
46-47	0.006472700012776179
48-49	0.0037651653669090192
50-51	0.005076627461000924
52-53	0.006007342495517761
54-55	0.0027498398747088345
56-57	0.002369092815133765
58-59	0.0025806189593421368
60-61	0.0032998078496506013
62-63	0.002284482357450416
64-65	0.0017345143825086493
66-67	0.0015229882383002774
68-69	0.0025806189593421368
70-71	0.0017345143825086493
72-73	0.001480683009458603
74-75	0.001819124840191998
76-77	0.003807470595750694
78-79	0.002834450332392183
80-81	0.0031305869342839036
82-83	0.002157566670925393
84-85	0.002961366018917206
86-87	0.001903735297875347
88-89	0.6026379848496515
90-91	0.6138911757215368
92-93	0.6099990946681029
94-95	0.5972229155579171
96-97	0.5939231077082665
98-99	0.5919770671815495
100	0.5927808665295413
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1181887.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	78.89877032971751
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.62892572457588	68.3491571465347
2	9.380256382284784	14.80181387879508
3	2.0312077350624382	4.80779377741911
4	0.7245790506999477	2.286735844275996
5	0.34950252223751144	1.37876596158372
6	0.2111485041005056	0.9995614398293521
7	0.14124011893500732	0.7800570193636588
8	0.09624905267391648	0.6075145521097775
9	0.07403543948936023	0.5257174622877657
>10	0.3505904172996798	4.691713783691961
>50	0.010119380231352433	0.520704414665576
>100	0.002145672409611226	0.25046471944333987
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	4.230522884167437E-5	0.0	0.0	0.0
16-17	0.0	8.461045768334874E-5	0.0	0.0	0.0
18-19	0.0	8.461045768334874E-5	0.0	0.0	0.0
20-21	0.0	8.461045768334874E-5	0.0	8.461045768334874E-5	0.0
22-23	0.0	8.461045768334874E-5	0.0	1.2691568652502312E-4	0.0
24-25	0.0	8.461045768334874E-5	0.0	2.5383137305004625E-4	0.0
26-27	0.0	8.461045768334874E-5	0.0	5.499679749417668E-4	0.0
28-29	8.461045768334874E-5	8.461045768334874E-5	0.0	0.0013960725517752543	0.0
30-31	8.461045768334874E-5	8.461045768334874E-5	0.0	0.0051612379186842736	0.0
32-33	8.461045768334874E-5	8.461045768334874E-5	0.0	0.0135376732293358	0.0
34-35	8.461045768334874E-5	8.461045768334874E-5	0.0	0.02369092815133765	0.0
36-37	8.461045768334874E-5	8.461045768334874E-5	0.0	0.039386168051598835	0.0
38-39	8.461045768334874E-5	8.461045768334874E-5	0.0	0.07356879295567173	0.0
40-41	8.461045768334874E-5	1.692209153666975E-4	0.0	0.12526578260019783	0.0
42-43	8.461045768334874E-5	1.692209153666975E-4	0.0	0.17899342322912426	0.0
44-45	8.461045768334874E-5	1.692209153666975E-4	0.0	0.22882898280461667	0.0
46-47	8.461045768334874E-5	1.692209153666975E-4	0.0	0.28314889663732656	0.0
48-49	8.461045768334874E-5	1.692209153666975E-4	0.0	0.35485625952396466	0.0
50-51	8.461045768334874E-5	1.692209153666975E-4	0.0	0.4365053511883962	0.0
52-53	8.461045768334874E-5	1.692209153666975E-4	0.0	0.5217503873043701	0.0
54-55	8.461045768334874E-5	1.692209153666975E-4	0.0	0.6099144842104194	0.0
56-57	8.461045768334874E-5	1.692209153666975E-4	0.0	0.704551281129245	0.0
58-59	8.461045768334874E-5	1.692209153666975E-4	0.0	0.8079875656471389	0.0
60-61	8.461045768334874E-5	1.692209153666975E-4	0.0	0.9196733697891593	0.0
62-63	8.461045768334874E-5	1.692209153666975E-4	0.0	1.0401586615302478	0.0
64-65	8.461045768334874E-5	1.692209153666975E-4	0.0	1.1748585101621392	0.0
66-67	8.461045768334874E-5	1.692209153666975E-4	0.0	1.3268611973902749	0.0
68-69	8.461045768334874E-5	1.692209153666975E-4	0.0	1.4835597650198369	0.0
70-71	8.461045768334874E-5	1.692209153666975E-4	0.0	1.6559112673208183	0.0
72-73	8.461045768334874E-5	1.692209153666975E-4	0.0	1.8662528651216235	0.0
74-75	8.461045768334874E-5	1.692209153666975E-4	0.0	2.0628452635488843	0.0
76-77	8.461045768334874E-5	1.692209153666975E-4	0.0	2.258633862628153	0.0
78-79	8.461045768334874E-5	1.692209153666975E-4	0.0	2.4793402414951684	0.0
80-81	8.461045768334874E-5	1.692209153666975E-4	0.0	2.7280103766265302	0.0
82-83	8.461045768334874E-5	1.692209153666975E-4	0.0	2.999736861476605	0.0
84-85	8.461045768334874E-5	2.5383137305004625E-4	0.0	3.266005971806103	0.0
86-87	8.461045768334874E-5	2.5383137305004625E-4	0.0	3.533967291289269	0.0
88	8.461045768334874E-5	3.38441830733395E-4	0.0	3.7589888034981347	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCTCGC	355	0.0	30.516813	1
GTACACG	140	1.8515493E-8	30.279879	1
GTACCGC	125	2.3807479E-7	30.054731	6
TCTGTCG	485	0.0	30.016003	8
GGTATCA	1045	0.0	29.748653	1
TCAACGC	1335	0.0	28.851988	4
ATCAACG	1410	0.0	27.65396	3
CAACGCA	1440	0.0	26.748198	5
AACGCAG	1510	0.0	26.123734	6
GTATCAA	1610	0.0	25.745213	1
GTACATG	3120	0.0	25.060698	1
CTGTCGC	565	0.0	24.10363	9
TACATGG	3100	0.0	23.80741	2
TCGCTCT	455	0.0	23.744312	4
ACATGGG	3060	0.0	23.642729	3
TATCAAC	1675	0.0	23.574291	2
TCTCGCT	500	0.0	23.50413	2
TATACTG	575	0.0	22.873539	5
TACACGG	145	2.5299107E-5	22.693642	2
CGCTCTG	445	0.0	22.166773	5
>>END_MODULE