FastQCFastQC Report
Wed 25 May 2016
SRR1033122_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033122_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1181887
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22930.19401177946791867No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18430.15593707351041175No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19200.045.593331
GTATCAA33250.036.801991
GTACACG959.3507697E-734.67881
TCAACGC36150.033.033554
ATCAACG36450.032.890653
CAACGCA37350.032.096755
TATCAAC39700.030.5533622
AACGCAG39400.030.5344286
GTACATG29400.026.57361
ACGCAGA45450.026.5732867
CGCAGAG46450.026.1012738
TCTGTCG5250.025.957718
TACATGG30300.025.2915122
ACATGGG30350.023.8556843
GTCTCGC3450.023.1911
CTGTCGC6100.023.111029
GCAGAGT52500.023.00399
GAGTACT36550.020.95694212-13
GTACTAG2701.1241355E-920.9173721
GTCTTAC4150.020.4133381
Produced by FastQC (version 0.11.5)