##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1033122_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1181887 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.78175409324242 34.0 31.0 34.0 31.0 34.0 2 32.87407594803903 34.0 31.0 34.0 31.0 34.0 3 32.96076528466765 34.0 31.0 34.0 31.0 34.0 4 36.38093827920943 37.0 37.0 37.0 35.0 37.0 5 36.31967946174211 37.0 37.0 37.0 35.0 37.0 6 36.408187077106355 37.0 37.0 37.0 35.0 37.0 7 36.38172600257047 37.0 37.0 37.0 35.0 37.0 8 36.37914199919282 37.0 37.0 37.0 35.0 37.0 9 38.20125274243646 39.0 39.0 39.0 37.0 39.0 10-11 38.18132401828601 39.0 39.0 39.0 37.0 39.0 12-13 38.14573093705236 39.0 39.0 39.0 37.0 39.0 14-15 39.6898599443094 41.0 40.0 41.0 37.0 41.0 16-17 39.70162079792738 41.0 40.0 41.0 37.0 41.0 18-19 39.71712185682726 41.0 40.0 41.0 37.0 41.0 20-21 39.701059407540654 41.0 40.0 41.0 37.0 41.0 22-23 39.620282226642644 41.0 40.0 41.0 37.0 41.0 24-25 39.56536284771725 41.0 40.0 41.0 37.0 41.0 26-27 39.45549870672915 41.0 39.5 41.0 36.5 41.0 28-29 39.36308885705655 41.0 39.0 41.0 36.0 41.0 30-31 39.26350742499071 41.0 39.0 41.0 36.0 41.0 32-33 39.266742928892526 41.0 39.0 41.0 35.5 41.0 34-35 39.19431045438354 41.0 39.0 41.0 35.0 41.0 36-37 39.09581965111724 41.0 39.0 41.0 35.0 41.0 38-39 38.99297563980313 41.0 39.0 41.0 35.0 41.0 40-41 38.874103869490064 41.0 39.0 41.0 35.0 41.0 42-43 38.756277884433956 41.0 39.0 41.0 35.0 41.0 44-45 38.607019114348496 41.0 39.0 41.0 35.0 41.0 46-47 38.391734996662116 41.0 39.0 41.0 35.0 41.0 48-49 38.20069854393863 41.0 38.0 41.0 34.0 41.0 50-51 38.06897275289431 41.0 38.0 41.0 34.0 41.0 52-53 37.91873123234285 40.0 38.0 41.0 34.0 41.0 54-55 37.778664542380106 40.0 37.5 41.0 34.0 41.0 56-57 37.62145831200445 40.0 37.0 41.0 33.0 41.0 58-59 37.43015914380986 40.0 37.0 41.0 33.0 41.0 60-61 37.21930269137405 40.0 36.0 41.0 33.0 41.0 62-63 36.96231704046157 39.0 36.0 41.0 33.0 41.0 64-65 36.68848544742433 39.0 35.0 41.0 32.0 41.0 66-67 36.3989598836437 39.0 35.0 41.0 32.0 41.0 68-69 36.06530319734459 37.5 35.0 40.5 31.5 41.0 70-71 35.71428782954716 37.0 35.0 40.0 31.0 41.0 72-73 35.36107512816369 36.5 35.0 39.0 31.0 41.0 74-75 35.029060307795916 36.0 35.0 39.0 31.0 41.0 76-77 34.70395731571631 36.0 35.0 39.0 31.0 40.5 78-79 34.37099655043164 35.0 35.0 37.0 31.0 39.5 80-81 34.0432647114318 35.0 35.0 37.0 30.0 39.0 82-83 33.73281286620464 35.0 34.0 36.5 30.0 39.0 84-85 33.468519410062044 35.0 34.0 36.0 30.0 37.5 86-87 33.24770261454775 35.0 34.0 36.0 30.0 37.0 88-89 33.04063924892989 35.0 34.0 35.5 30.0 37.0 90-91 32.84463193181751 35.0 34.0 35.0 29.0 36.0 92-93 32.68379041312748 35.0 34.0 35.0 29.0 36.0 94-95 32.53793340649318 35.0 34.0 35.0 29.0 36.0 96-97 32.41224076413397 35.0 34.0 35.0 29.0 36.0 98-99 32.31160635492226 35.0 34.0 35.0 29.0 35.5 100 32.2178473915019 35.0 34.0 35.0 29.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.11395982212786038 1101 2 0.16653557104251604 1101 3 0.18104897521144636 1101 4 0.11548010837011446 1101 5 0.12034125242116289 1101 6 0.11450527828612422 1101 7 0.16761916988283332 1101 8 0.12227842167103375 1101 9 0.2195577790880776 1101 10-11 0.1551855974704992 1101 12-13 0.19675948510911923 1101 14-15 0.27650380511293093 1101 16-17 0.30422139703452444 1101 18-19 0.3058697356894129 1101 20-21 0.3213554266245211 1101 22-23 0.3213533719612087 1101 24-25 0.21357339551772725 1101 26-27 0.22856151768866084 1101 28-29 0.265089308857128 1101 30-31 0.2412534806657476 1101 32-33 0.33540144576197406 1101 34-35 0.296282797788308 1101 36-37 0.356335326925759 1101 38-39 0.31820904104183967 1101 40-41 0.33266800187315226 1101 42-43 0.2679689430371539 1101 44-45 0.22335367638705605 1101 46-47 0.3729817645318789 1101 48-49 0.34047826142329995 1101 50-51 0.3707856703484822 1101 52-53 0.40768969826769563 1101 54-55 0.3563967791620115 1101 56-57 0.45441100350533503 1101 58-59 0.3668986753687804 1101 60-61 0.466235614247168 1101 62-63 0.499692059928428 1101 64-65 0.5964475730089305 1101 66-67 0.4965833536057147 1101 68-69 0.4845270873681642 1101 70-71 0.48526070022671064 1101 72-73 0.5488285109281676 1101 74-75 0.5310838147906054 1101 76-77 0.5345093075338667 1101 78-79 0.4936545340306324 1101 80-81 0.44730975660351646 1101 82-83 0.44051833231539916 1101 84-85 0.4111362807848238 1101 86-87 0.44549605664914793 1101 88-89 0.4010298703048534 1101 90-91 0.41818545910986415 1101 92-93 0.36125244387994115 1101 94-95 0.43336257795431976 1101 96-97 0.4656060498106882 1101 98-99 0.41557129372945667 1101 100 0.378767209477914 1102 1 0.0568525449821351 1102 2 0.007866631401277857 1102 3 0.05943073015738065 1102 4 0.046276092781695866 1102 5 0.10695488301443845 1102 6 0.04573893120613093 1102 7 0.07655147634751103 1102 8 0.08954919984241627 1102 9 0.1140309043878176 1102 10-11 0.11136192226405939 1102 12-13 0.15218198030323293 1102 14-15 0.10187769734008612 1102 16-17 0.17492138454122141 1102 18-19 0.11443570635837119 1102 20-21 0.10266403934431878 1102 22-23 0.06802511654979781 1102 24-25 0.14207121725041816 1102 26-27 0.1263461734982414 1102 28-29 0.10770496255897655 1102 30-31 0.04714407159883649 1102 32-33 0.11612065683023332 1102 34-35 0.13722354327560282 1102 36-37 0.13805964412661353 1102 38-39 0.07645643256171297 1102 40-41 0.26359093433276115 1102 42-43 0.24164152991596666 1102 44-45 0.1831635364217874 1102 46-47 0.1794369720214064 1102 48-49 0.16233750626376775 1102 50-51 0.11423253150761781 1102 52-53 0.24291737858429485 1102 54-55 0.23659751828176212 1102 56-57 0.18731654206731463 1102 58-59 0.1667644731465785 1102 60-61 0.22536458943385895 1102 62-63 0.22287075844898396 1102 64-65 0.13893768919662364 1102 66-67 0.12435616093082302 1102 68-69 0.2170662901344258 1102 70-71 0.18498806418072178 1102 72-73 0.14660208397165775 1102 74-75 0.16885005305798018 1102 76-77 0.08629746146744566 1102 78-79 0.11469728126699152 1102 80-81 0.10732958467958298 1102 82-83 0.1291185991078052 1102 84-85 0.196482042936573 1102 86-87 0.26941790501273033 1102 88-89 0.2572898196062283 1102 90-91 0.2329710781125769 1102 92-93 0.26287919243817015 1102 94-95 0.19816230229569953 1102 96-97 0.3308809225603824 1102 98-99 0.21884695637812257 1102 100 0.20656480189810367 1103 1 0.11991475737000457 1103 2 0.14400161736077877 1103 3 0.0854184473300208 1103 4 0.050695483645988304 1103 5 0.023236360366666986 1103 6 0.10989518363548711 1103 7 0.045572130382467435 1103 8 0.07529482510431507 1103 9 0.1329143767988512 1103 10-11 0.042430004925698483 1103 12-13 0.029016014239331867 1103 14-15 0.06237849085092506 1103 16-17 0.07353989827739582 1103 18-19 0.14141336860146936 1103 20-21 0.09243903114375485 1103 22-23 0.109324843703412 1103 24-25 0.09943930199776219 1103 26-27 0.13413528353363802 1103 28-29 0.1400350782390163 1103 30-31 0.10519683201165009 1103 32-33 0.07129971345296582 1103 34-35 0.1552877181096548 1103 36-37 0.1370459980153953 1103 38-39 0.10967922424093501 1103 40-41 0.027425800649410803 1103 42-43 0.023059534743403276 1103 44-45 0.0101791203301147 1103 46-47 0.07977049306698802 1103 48-49 -0.01497263417911654 1103 50-51 0.0788076287292867 1103 52-53 0.04633563748688374 1103 54-55 0.05431452027075068 1103 56-57 0.03430205409511444 1103 58-59 0.021505344927739145 1103 60-61 -0.05523715154978248 1103 62-63 -0.12015819663849214 1103 64-65 -0.02172635708296866 1103 66-67 -0.043919142774321074 1103 68-69 -0.08511230953224214 1103 70-71 -0.004185490008396187 1103 72-73 0.0146762132428222 1103 74-75 -0.016038163353371715 1103 76-77 -0.06602362885627855 1103 78-79 -0.012890540569443942 1103 80-81 0.039526216098067835 1103 82-83 0.0077963844067170385 1103 84-85 -0.027209707015551032 1103 86-87 -0.025750495622077096 1103 88-89 -0.043435811457683826 1103 90-91 -0.02416473643530992 1103 92-93 -0.08859889926779374 1103 94-95 0.011447778487529092 1103 96-97 0.02572054235776733 1103 98-99 -0.03103349557228796 1103 100 -0.18576781661183617 1104 1 0.0378494297173404 1104 2 0.08293446377992808 1104 3 0.06160248285672054 1104 4 0.02334037442843595 1104 5 -0.0011995179949551016 1104 6 0.026060283610689794 1104 7 0.032046736455370706 1104 8 0.08091913474566326 1104 9 0.11429441782588157 1104 10-11 0.08875447912600976 1104 12-13 0.13005634245556053 1104 14-15 0.1173479460029796 1104 16-17 0.08450110293915003 1104 18-19 0.09446898051283625 1104 20-21 0.06273952031216368 1104 22-23 0.032906368015311216 1104 24-25 0.053407877999205766 1104 26-27 -0.010828761460835779 1104 28-29 0.11380708518205296 1104 30-31 0.036144060433009884 1104 32-33 0.042947106433288695 1104 34-35 0.10479854969595692 1104 36-37 0.12208916656434354 1104 38-39 0.0824988180282702 1104 40-41 0.0732599793198645 1104 42-43 0.13696025835196934 1104 44-45 0.18199670785068633 1104 46-47 0.08255446998364846 1104 48-49 0.10900450790514782 1104 50-51 0.06299982457699116 1104 52-53 0.04605385844673293 1104 54-55 0.020602475907466555 1104 56-57 0.13905059447969137 1104 58-59 0.08806718979148087 1104 60-61 0.09477637384413384 1104 62-63 0.13352183762583536 1104 64-65 0.17966112291071568 1104 66-67 0.18591111353189405 1104 68-69 0.18413883449066049 1104 70-71 0.1464648560162658 1104 72-73 0.13576230237516285 1104 74-75 0.16419334630600702 1104 76-77 0.16895191272303833 1104 78-79 0.17144367191450272 1104 80-81 0.125012341298131 1104 82-83 0.11758876960993092 1104 84-85 0.013721516356319796 1104 86-87 0.0604344415975504 1104 88-89 0.013477919071391398 1104 90-91 -0.05884046511590668 1104 92-93 0.039807809327591315 1104 94-95 4.7530266417794564E-4 1104 96-97 0.04705685127776604 1104 98-99 0.070452043527375 1104 100 0.13468257900593272 1105 1 0.037562567984693374 1105 2 0.020676214191716724 1105 3 0.02114675312776626 1105 4 0.045823548291323846 1105 5 0.07132611962978785 1105 6 0.049247759577028205 1105 7 -0.04565269413951256 1105 8 -0.05726288159414139 1105 9 -0.022009116845318033 1105 10-11 -0.01525294843369096 1105 12-13 0.04226996910758629 1105 14-15 0.0664757423907858 1105 16-17 0.05115368356374006 1105 18-19 0.041372827338953755 1105 20-21 0.030852628145446204 1105 22-23 0.06826747449962056 1105 24-25 0.021678297105225397 1105 26-27 0.06595507639956111 1105 28-29 0.09705086418952646 1105 30-31 0.01626166346161284 1105 32-33 0.018022523308161453 1105 34-35 0.04080422112719617 1105 36-37 -0.009082730445101106 1105 38-39 -0.1342550381542651 1105 40-41 -0.014222053265164902 1105 42-43 0.06495025212121419 1105 44-45 0.09285570965410983 1105 46-47 0.1330441912856486 1105 48-49 0.06763935451122904 1105 50-51 0.04393585749514983 1105 52-53 0.0027572722041711017 1105 54-55 0.028822761041851663 1105 56-57 0.016292794205369887 1105 58-59 0.006134235239009911 1105 60-61 -0.09833555158414953 1105 62-63 -0.1056663539525644 1105 64-65 -0.12277082711159437 1105 66-67 -0.05574367767594168 1105 68-69 -0.12185575317901254 1105 70-71 -0.04104504019942112 1105 72-73 -0.10600353208402424 1105 74-75 -0.13785714958436301 1105 76-77 -0.12183386222519488 1105 78-79 -0.04107614622132871 1105 80-81 0.01450220037602179 1105 82-83 -0.07231678193572577 1105 84-85 -0.06162595648434177 1105 86-87 -0.0648773534577316 1105 88-89 -0.21791513908031845 1105 90-91 -0.19932097394076465 1105 92-93 -0.06032390030664203 1105 94-95 -0.1107052104790327 1105 96-97 -0.09725619198592028 1105 98-99 -0.13819616939600365 1105 100 -0.037647293479395216 1106 1 0.030209361805624724 1106 2 0.025775437181174254 1106 3 -0.0070952471313319165 1106 4 -0.018444194520817803 1106 5 -0.05068703607330605 1106 6 -0.012548783860857782 1106 7 0.008466279937735521 1106 8 -0.04544067158018095 1106 9 -0.019705456411685418 1106 10-11 0.02188030444199285 1106 12-13 -0.04451763729669267 1106 14-15 -0.013130580061286423 1106 16-17 -0.00942771552277577 1106 18-19 0.04897441973813699 1106 20-21 0.011302684495021253 1106 22-23 0.024040116364673736 1106 24-25 0.04300103652275311 1106 26-27 0.061877646135215514 1106 28-29 -0.0032148603628172623 1106 30-31 0.04867314299605141 1106 32-33 0.131656783852641 1106 34-35 0.10883866791264296 1106 36-37 0.12309526604242649 1106 38-39 0.06836626442203908 1106 40-41 0.13574818909160768 1106 42-43 0.12446261639762923 1106 44-45 0.1887249981103878 1106 46-47 0.16615504849085028 1106 48-49 0.17894414193646213 1106 50-51 0.09954953217933138 1106 52-53 0.13100638312760537 1106 54-55 0.11869717502084143 1106 56-57 0.14318503452245324 1106 58-59 -0.04006901092537163 1106 60-61 0.04577618520048787 1106 62-63 0.08535482511247494 1106 64-65 0.08692704757901026 1106 66-67 0.1156413531093321 1106 68-69 0.1697861966235905 1106 70-71 0.14960891688528477 1106 72-73 0.14953328898146623 1106 74-75 0.21250184155847762 1106 76-77 0.07228776130506986 1106 78-79 0.10018353231819788 1106 80-81 0.017689823534190907 1106 82-83 0.04326317066633578 1106 84-85 -0.026035133332413807 1106 86-87 0.05268433155541885 1106 88-89 0.03613087763267231 1106 90-91 -0.010689172906893418 1106 92-93 -0.08361230008572562 1106 94-95 -0.05446716102192539 1106 96-97 0.059035723188728184 1106 98-99 -0.032987559063329286 1106 100 -0.0715992546103621 1107 1 -0.0076392215676008846 1107 2 -0.014299608384703788 1107 3 0.01840986296723912 1107 4 -0.03406478064949425 1107 5 -0.10514889369628833 1107 6 -0.16789285045535252 1107 7 -0.08946811984264258 1107 8 -0.04065589572801542 1107 9 -0.09121753666495636 1107 10-11 -0.011433506343436761 1107 12-13 -0.015341778631018599 1107 14-15 0.03499504833067846 1107 16-17 -0.007526894524801264 1107 18-19 -0.0781008064033486 1107 20-21 -6.968352439287173E-4 1107 22-23 -0.08890498896949595 1107 24-25 -0.15811846854084877 1107 26-27 -0.041927212463257035 1107 28-29 -0.1269400427616958 1107 30-31 -0.06828586825218963 1107 32-33 0.03703675855931721 1107 34-35 0.020718249779797304 1107 36-37 0.010191084809491713 1107 38-39 -0.0025704898476845983 1107 40-41 -0.05532803196732772 1107 42-43 0.045755537738315866 1107 44-45 0.0033705078129031563 1107 46-47 -0.1275018113598705 1107 48-49 -0.0762837129495395 1107 50-51 -0.1334192741012643 1107 52-53 -0.08772879780946852 1107 54-55 -0.047136837461494 1107 56-57 -0.06724143919902303 1107 58-59 -0.10324483583264055 1107 60-61 -0.020021329989994285 1107 62-63 -0.026197905353114948 1107 64-65 -0.0762685546565578 1107 66-67 -0.09308493917664862 1107 68-69 -0.013192625711347716 1107 70-71 -0.1224296670580074 1107 72-73 -0.044520200251987774 1107 74-75 -0.0822187264489358 1107 76-77 -0.10669976687603366 1107 78-79 -0.14460740285007034 1107 80-81 -0.16851913651911588 1107 82-83 -0.0833650751164896 1107 84-85 0.0044316657563641115 1107 86-87 -0.06012048532276282 1107 88-89 -0.09229197757021979 1107 90-91 -0.11829439959934263 1107 92-93 -0.1654195784610195 1107 94-95 -0.14410256708932678 1107 96-97 -0.0682113204828596 1107 98-99 -0.02075284517057696 1107 100 -0.0025639582896133106 1108 1 0.08040069083147472 1108 2 0.04238213734775087 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2214 4 -0.0325718641490127 2214 5 -0.06011253373540626 2214 6 -0.0259272817811933 2214 7 -0.04059306589003597 2214 8 -0.0034384298342615693 2214 9 0.028423125299404717 2214 10-11 -0.03584794979837369 2214 12-13 0.013333418466736191 2214 14-15 0.009910090044918718 2214 16-17 -0.020923668841717813 2214 18-19 -0.07525443065279092 2214 20-21 -0.055720710980821764 2214 22-23 -0.059897334528450585 2214 24-25 -0.022634042405385912 2214 26-27 -0.14201271710454932 2214 28-29 -0.1156360833231247 2214 30-31 -0.08445771892229459 2214 32-33 -0.10004015737295902 2214 34-35 -0.02346060516827464 2214 36-37 -0.13099715652667498 2214 38-39 -0.08733768775405792 2214 40-41 -0.0816030891980759 2214 42-43 -0.030474581749636798 2214 44-45 -0.06381647144198155 2214 46-47 -0.028804869311798598 2214 48-49 -0.11412334120923617 2214 50-51 -0.03517239242547987 2214 52-53 -0.12294400725486554 2214 54-55 -0.07209422172972779 2214 56-57 -0.1459585690841223 2214 58-59 -0.10864610665561969 2214 60-61 -0.2020247884205375 2214 62-63 -0.3045556698848131 2214 64-65 -0.15662079007023522 2214 66-67 -0.17793340746890607 2214 68-69 -0.08074470705275871 2214 70-71 -0.09189016828457852 2214 72-73 -0.09105330120210198 2214 74-75 -0.14672173413616463 2214 76-77 -0.1053979382240513 2214 78-79 -0.19722389950909758 2214 80-81 -0.11314723230616863 2214 82-83 -0.14231396901971038 2214 84-85 -0.15723056632453591 2214 86-87 -0.1611782296392974 2214 88-89 -0.320047385124127 2214 90-91 -0.17242921829125635 2214 92-93 -0.15595984213219793 2214 94-95 -0.2178729939569095 2214 96-97 -0.2772785003595004 2214 98-99 -0.2739274440090185 2214 100 -0.3889785547240976 2215 1 0.021198471501818972 2215 2 -0.04871755855647564 2215 3 -0.021491282520955224 2215 4 -0.01627701499589307 2215 5 -0.06041587460269682 2215 6 -0.008612603377414985 2215 7 -0.04716270618175855 2215 8 -0.004988076165346911 2215 9 -0.0520958026216789 2215 10-11 -0.06391224159123254 2215 12-13 -0.07302127530996927 2215 14-15 -0.09197927060586863 2215 16-17 -0.12857925113722501 2215 18-19 -0.08808968407738149 2215 20-21 -0.11120989960119232 2215 22-23 -0.12203554449706644 2215 24-25 -0.09923378801998695 2215 26-27 -0.11054167403288773 2215 28-29 -0.06754923541448932 2215 30-31 -0.03470752904829766 2215 32-33 -0.07520578240155373 2215 34-35 -0.133948512711072 2215 36-37 -0.09351214055895696 2215 38-39 -0.07875378174854575 2215 40-41 -0.10530119411833283 2215 42-43 -0.04383050170059022 2215 44-45 -0.03986123595247193 2215 46-47 -0.15389797619942414 2215 48-49 -0.14912672691423978 2215 50-51 -0.09613269732194141 2215 52-53 -0.02513442113441755 2215 54-55 -0.0878956272548379 2215 56-57 -0.12434015633923678 2215 58-59 -0.07381177743697975 2215 60-61 -0.1127947617924221 2215 62-63 -0.19675482254350385 2215 64-65 -0.22631507005888807 2215 66-67 -0.20607639402673783 2215 68-69 -0.19234600826155202 2215 70-71 -0.15041276557784045 2215 72-73 -0.11723074874655026 2215 74-75 -0.11419700476379546 2215 76-77 -0.21136432823897167 2215 78-79 -0.1401647045748433 2215 80-81 -0.15830590291367486 2215 82-83 -0.2241274715772903 2215 84-85 -0.18781537823489458 2215 86-87 -0.15177969555806214 2215 88-89 -0.12132114225012458 2215 90-91 -0.1558891342891684 2215 92-93 -0.040464065112900016 2215 94-95 -0.01948372023638001 2215 96-97 -0.005732834519427854 2215 98-99 -0.043798787218847224 2215 100 -0.08951248955385438 2216 1 -0.06800228596893731 2216 2 -0.12258221869951313 2216 3 -0.15820115320343575 2216 4 -0.08034367311531554 2216 5 -0.06982751803009535 2216 6 -0.053218829468228535 2216 7 -0.13850229632978284 2216 8 -0.11025953206156203 2216 9 -0.14973667624256848 2216 10-11 -0.13824122506964187 2216 12-13 -0.14273409652469837 2216 14-15 -0.22407406186827217 2216 16-17 -0.18396800065211494 2216 18-19 -0.14658554501601628 2216 20-21 -0.20354873782262928 2216 22-23 -0.18513782210697372 2216 24-25 -0.27700118974976107 2216 26-27 -0.33695712387211074 2216 28-29 -0.24878455205528383 2216 30-31 -0.2913769793449035 2216 32-33 -0.277955901947486 2216 34-35 -0.3361890460591894 2216 36-37 -0.3096468566980093 2216 38-39 -0.2766170281309783 2216 40-41 -0.39147423425804107 2216 42-43 -0.36564555547894173 2216 44-45 -0.292701751282074 2216 46-47 -0.3276064469817044 2216 48-49 -0.21135081836514757 2216 50-51 -0.21008271152884817 2216 52-53 -0.15638784292018926 2216 54-55 -0.1750987548536571 2216 56-57 -0.2655000810772705 2216 58-59 -0.23603625871158584 2216 60-61 -0.17185586708986733 2216 62-63 -0.1080617021520851 2216 64-65 -0.19854727850540854 2216 66-67 -0.17442335963909272 2216 68-69 -0.21650641464842124 2216 70-71 -0.11490112630855975 2216 72-73 -0.10077542427463015 2216 74-75 -0.2246464021829908 2216 76-77 -0.1677114376529545 2216 78-79 -0.211987338258389 2216 80-81 -0.17122877499605949 2216 82-83 -0.17066250200086586 2216 84-85 -0.12775589113893204 2216 86-87 -0.2073455982857837 2216 88-89 -0.21268953603789953 2216 90-91 -0.15428869144945168 2216 92-93 -0.18432265266903158 2216 94-95 -0.2073111721407983 2216 96-97 -0.09945511372329463 2216 98-99 -0.07841015031969789 2216 100 -0.1180895984134196 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9.0 3 7.0 4 6.0 5 15.0 6 31.0 7 73.0 8 174.0 9 347.0 10 635.0 11 1096.0 12 1757.0 13 2686.0 14 2743.0 15 3185.0 16 3857.0 17 5503.0 18 6438.0 19 3599.0 20 2371.0 21 2219.0 22 2356.0 23 2430.0 24 2705.0 25 3386.0 26 4222.0 27 5380.0 28 6633.0 29 8795.0 30 11215.0 31 14220.0 32 18044.0 33 23671.0 34 32863.0 35 50904.0 36 95219.0 37 231540.0 38 422393.0 39 208193.0 40 967.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.388193229416316 19.350549705542107 12.760519438285792 23.50073762675578 2 16.717334743521945 19.874267583289264 39.79007932310947 23.618318350079324 3 18.556596358196682 25.178295386953238 29.678641020672874 26.586467234177206 4 12.585922137874784 16.913081148403737 38.53897675237164 31.962019961349846 5 12.103065611137396 36.80383863707731 34.89129137567808 16.20180437610721 6 30.29152582527065 37.96105374042314 17.95369261814813 13.793727816158086 7 26.79817810904777 32.02693406687318 22.647563267388644 18.527324556690406 8 25.199597927362937 34.79062101269507 21.063800263310196 18.945980796631794 9 24.563790416334573 16.42400366871255 22.058049591923417 36.954156323029466 10-11 23.7101425731533 26.13081058259077 28.86834542630858 21.290701417947353 12-13 25.09571558025429 23.447503864582657 29.094194284225143 22.362586270937914 14-15 22.332897448274434 25.482269236512984 26.714621074020222 25.470212241192364 16-17 21.15896020516344 29.010260710203262 26.78961694307493 23.04116214155837 18-19 20.778729598823574 27.120666832221197 30.064350510814702 22.036253058140524 20-21 22.409375854036806 26.818722940518004 29.33216119645956 21.43974000898563 22-23 21.640729937197648 27.150668530924182 29.460204377338695 21.74839715453947 24-25 21.46895786436165 26.866223504730364 29.727922036440923 21.93689659446706 26-27 21.41933971782936 26.397706691959844 29.92151867352978 22.261434916681015 28-29 21.55678260865886 26.699179672080376 29.73548031428768 22.008557404973075 30-31 21.94851928294504 27.65111134716326 28.954485994795476 21.445883375096226 32-33 21.10282707926308 26.92438708672759 29.998548566529603 21.974237267479722 34-35 21.003968937425867 26.231168282692952 30.138322483888754 22.626540295992427 36-37 22.262958895878164 27.25685474230316 28.8485048526434 21.631681509175273 38-39 22.02141592208853 27.348610673262257 29.08536959497629 21.54460380967292 40-41 21.361442744383112 26.98120904660583 29.51467001149361 22.142678197517448 42-43 21.286867490935066 27.12532482067952 29.244545699587892 22.343261988797522 44-45 21.94867754375958 27.28255193897905 28.94454002828304 21.824230488978333 46-47 21.693349146635942 28.091770749988047 28.339294350411333 21.875585752964678 48-49 21.573065844387504 27.483380664028783 28.789530156817484 22.15402333476623 50-51 21.725201768830207 27.067400689457926 28.75732570202353 22.45007183968834 52-53 21.78963271588355 27.25423889438651 28.42674900270641 22.529379387023525 54-55 21.927141791616233 28.199926640033134 28.05358746737618 21.81934410097445 56-57 21.299090076811257 27.643041719055926 28.214387853701545 22.84348035043127 58-59 21.13828257932086 26.857997913488457 29.013890616367018 22.989828890823663 60-61 21.994425825317144 27.70545884984842 28.09729253163818 22.20282279319626 62-63 21.72761224969836 27.5218424299037 28.195475998287467 22.55506932211048 64-65 21.541659915243017 27.098035748206517 28.678947700193635 22.681356636356828 66-67 21.41213878554609 27.302494921231496 28.15025370574533 23.13511258747708 68-69 22.37565975506778 27.538671504165407 28.03258881204321 22.053079928723605 70-71 22.405888566851132 27.129365965427223 28.265095502221666 22.199649965499983 72-73 21.875087254977448 27.429157419401783 28.633128039117306 22.062627286503464 74-75 21.834944744953606 27.271269660364013 28.444778374847594 22.449007219834787 76-77 21.950066948279545 27.49321527309187 27.91068486080469 22.646032917823895 78-79 22.18685973653138 27.828433610164765 28.020372514619634 21.96433413868421 80-81 21.846681817643386 27.196835217052122 28.49214219355232 22.464340771752173 82-83 21.52048302663297 27.393070876001897 28.51640732019552 22.570038777169614 84-85 21.70066749415974 27.43249543313509 28.150163764095094 22.716673308610076 86-87 22.08348439844638 27.481384618053102 28.049417646633284 22.385713336867237 88-89 21.433225003744013 27.388320541642308 28.220083645898463 22.958370808715216 90-91 21.248012711875162 27.09298773909858 28.375597667120463 23.2834018819058 92-93 21.702413174863587 27.56634094460807 27.89962153742278 22.83162434310556 94-95 21.477307052197038 27.219564983792864 28.185985631452077 23.117142332558018 96-97 21.26057736484114 27.157164771251395 28.34446101869299 23.237796845214476 98-99 21.124100696597896 27.28678799242229 28.301690432334052 23.287420878645758 100 21.587850615160335 27.27477330743125 28.020614491909974 23.116761585498445 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 39.0 1 31.5 2 175.0 3 293.0 4 171.0 5 103.0 6 198.5 7 397.5 8 632.5 9 738.5 10 618.0 11 486.5 12 502.0 13 558.0 14 653.0 15 741.0 16 839.0 17 972.5 18 1096.5 19 1264.0 20 1543.0 21 1917.5 22 2422.0 23 3321.0 24 4492.5 25 5764.0 26 7528.5 27 9799.0 28 12591.0 29 15656.0 30 18614.0 31 21642.0 32 24769.0 33 28020.0 34 30962.0 35 33080.0 36 34822.5 37 36492.5 38 37857.0 39 38459.5 40 38988.5 41 39270.0 42 39293.5 43 39419.0 44 38807.5 45 38425.5 46 38372.5 47 38113.5 48 38541.0 49 39067.5 50 38673.5 51 37446.5 52 34779.0 53 32665.0 54 31896.0 55 30971.5 56 29622.0 57 28164.5 58 26048.5 59 23003.0 60 20027.5 61 17143.5 62 14263.5 63 11458.0 64 9090.0 65 7124.0 66 5407.0 67 4023.5 68 3003.5 69 2248.5 70 1672.0 71 1231.5 72 932.0 73 755.0 74 592.0 75 395.0 76 237.5 77 164.0 78 121.5 79 82.5 80 49.0 81 25.0 82 14.5 83 6.5 84 2.5 85 1.5 86 0.5 87 0.0 88 1.0 89 1.0 90 0.0 91 0.5 92 1.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0907870210942332 2 0.001015325492200185 3 0.0 4 2.5383137305004625E-4 5 0.03604405497310657 6 1.692209153666975E-4 7 0.003215197391967253 8 9.307150345168361E-4 9 8.461045768334874E-5 10-11 2.1152614420837185E-4 12-13 0.0 14-15 4.230522884167437E-5 16-17 0.0 18-19 4.230522884167437E-5 20-21 0.0 22-23 3.8074705957506934E-4 24-25 1.2691568652502312E-4 26-27 0.001142241178725208 28-29 0.008757182370226595 30-31 0.0351556451674314 32-33 0.025087000703112903 34-35 0.028471419010446856 36-37 0.02660998894141318 38-39 0.02449472749932946 40-41 0.030544375223688896 42-43 0.023140960176395883 44-45 0.021998718997670676 46-47 0.015356798069527798 48-49 0.011083969956518685 50-51 0.0075303307338180385 52-53 0.0053727640628926454 54-55 0.003680554909225671 56-57 0.0024537032728171137 58-59 8.461045768334874E-4 60-61 6.7688366146679E-4 62-63 0.0010999359498835338 64-65 0.0031728921631255782 66-67 5.076627461000925E-4 68-69 7.614941191501387E-4 70-71 2.961366018917206E-4 72-73 5.076627461000925E-4 74-75 3.38441830733395E-4 76-77 3.8074705957506934E-4 78-79 4.230522884167437E-5 80-81 4.230522884167437E-4 82-83 5.076627461000925E-4 84-85 3.38441830733395E-4 86-87 1.2691568652502312E-4 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1181887.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 77.0512398866743 #Duplication Level Percentage of deduplicated Percentage of total 1 85.83599765437462 66.13770046179232 2 9.847379224056343 15.175055576956357 3 2.1611390337373613 4.9955532635085875 4 0.8037507329460017 2.4771996213325065 5 0.39970089409562076 1.5398724736939933 6 0.22429905796155486 1.0369512312810496 7 0.14650652725284768 0.7901956703425926 8 0.10289087596309185 0.6342295652785793 9 0.07367593223328298 0.510913973554294 >10 0.38757895563917605 5.093731454439789 >50 0.012574160440502203 0.6282969845491211 >100 0.00406725407313859 0.502941563194393 >500 2.1984861321392782E-4 0.1270470617662427 >1k 2.1984861321392782E-4 0.35031109831011986 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2293 0.19401177946791867 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1843 0.15593707351041175 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 8.461045768334874E-5 0.0 0.0 0.0 3 0.0 8.461045768334874E-5 0.0 0.0 0.0 4 0.0 8.461045768334874E-5 0.0 0.0 0.0 5 0.0 1.692209153666975E-4 0.0 0.0 0.0 6 0.0 1.692209153666975E-4 0.0 0.0 0.0 7 0.0 1.692209153666975E-4 0.0 0.0 0.0 8 0.0 1.692209153666975E-4 0.0 0.0 0.0 9 0.0 1.692209153666975E-4 0.0 8.461045768334874E-5 0.0 10-11 0.0 1.692209153666975E-4 0.0 8.461045768334874E-5 0.0 12-13 0.0 1.692209153666975E-4 0.0 1.2691568652502312E-4 0.0 14-15 0.0 1.692209153666975E-4 0.0 1.692209153666975E-4 0.0 16-17 0.0 1.692209153666975E-4 0.0 1.692209153666975E-4 0.0 18-19 0.0 1.692209153666975E-4 0.0 1.692209153666975E-4 0.0 20-21 0.0 1.692209153666975E-4 0.0 3.38441830733395E-4 0.0 22-23 0.0 1.692209153666975E-4 0.0 3.8074705957506934E-4 0.0 24-25 8.461045768334874E-5 1.692209153666975E-4 0.0 5.076627461000925E-4 0.0 26-27 8.461045768334874E-5 1.692209153666975E-4 0.0 7.191888903084643E-4 0.0 28-29 8.461045768334874E-5 2.5383137305004625E-4 0.0 0.0015229882383002774 0.0 30-31 8.461045768334874E-5 2.5383137305004625E-4 0.0 0.00533045883405097 0.0 32-33 8.461045768334874E-5 2.5383137305004625E-4 0.0 0.013706894144702497 0.0 34-35 8.461045768334874E-5 2.5383137305004625E-4 0.0 0.023733233380179325 0.0 36-37 8.461045768334874E-5 2.5383137305004625E-4 0.0 0.039470778509282184 0.0 38-39 8.461045768334874E-5 3.38441830733395E-4 0.0 0.07356879295567173 0.0 40-41 8.461045768334874E-5 4.230522884167437E-4 0.0 0.12526578260019783 0.0 42-43 8.461045768334874E-5 4.230522884167437E-4 0.0 0.1794587807463827 0.0 44-45 8.461045768334874E-5 4.230522884167437E-4 0.0 0.2296327821526085 0.0 46-47 8.461045768334874E-5 4.230522884167437E-4 0.0 0.2841219169006851 0.0 48-49 8.461045768334874E-5 4.230522884167437E-4 0.0 0.35608311116037317 0.0 50-51 8.461045768334874E-5 4.230522884167437E-4 0.0 0.43777450805364637 0.0 52-53 8.461045768334874E-5 4.230522884167437E-4 0.0 0.5238233435176121 0.0 54-55 8.461045768334874E-5 4.230522884167437E-4 0.0 0.6122412717967115 0.0 56-57 8.461045768334874E-5 4.230522884167437E-4 0.0 0.7075972576058456 0.0 58-59 8.461045768334874E-5 4.230522884167437E-4 0.0 0.8125565303620397 0.0 60-61 8.461045768334874E-5 4.230522884167437E-4 0.0 0.9249615233943684 0.0 62-63 8.461045768334874E-5 4.230522884167437E-4 0.0 1.0457429517373489 0.0 64-65 8.461045768334874E-5 4.230522884167437E-4 0.0 1.1812465997172317 0.0 66-67 8.461045768334874E-5 4.230522884167437E-4 0.0 1.333630034004943 0.0 68-69 8.461045768334874E-5 4.230522884167437E-4 0.0 1.4914285375843883 0.0 70-71 8.461045768334874E-5 4.230522884167437E-4 0.0 1.6646261444622032 0.0 72-73 8.461045768334874E-5 5.076627461000925E-4 0.0 1.8766176461878334 0.0 74-75 8.461045768334874E-5 5.076627461000925E-4 0.0 2.0749022537687614 0.0 76-77 8.461045768334874E-5 5.076627461000925E-4 0.0 2.2723407567728557 0.0 78-79 8.461045768334874E-5 5.076627461000925E-4 0.0 2.4961354173453127 0.0 80-81 8.461045768334874E-5 5.076627461000925E-4 0.0 2.748020749868642 0.0 82-83 8.461045768334874E-5 5.076627461000925E-4 0.0 3.0245277255778262 0.0 84-85 8.461045768334874E-5 5.076627461000925E-4 0.0 3.296423431343267 0.0 86-87 8.461045768334874E-5 5.076627461000925E-4 0.0 3.567726863904925 0.0 88 8.461045768334874E-5 5.076627461000925E-4 0.0 3.7964712362518584 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1920 0.0 45.59333 1 GTATCAA 3325 0.0 36.80199 1 GTACACG 95 9.3507697E-7 34.6788 1 TCAACGC 3615 0.0 33.03355 4 ATCAACG 3645 0.0 32.89065 3 CAACGCA 3735 0.0 32.09675 5 TATCAAC 3970 0.0 30.553362 2 AACGCAG 3940 0.0 30.534428 6 GTACATG 2940 0.0 26.5736 1 ACGCAGA 4545 0.0 26.573286 7 CGCAGAG 4645 0.0 26.101273 8 TCTGTCG 525 0.0 25.95771 8 TACATGG 3030 0.0 25.291512 2 ACATGGG 3035 0.0 23.855684 3 GTCTCGC 345 0.0 23.191 1 CTGTCGC 610 0.0 23.11102 9 GCAGAGT 5250 0.0 23.0039 9 GAGTACT 3655 0.0 20.956942 12-13 GTACTAG 270 1.1241355E-9 20.917372 1 GTCTTAC 415 0.0 20.413338 1 >>END_MODULE