FastQCFastQC Report
Wed 25 May 2016
SRR1033121_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033121_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2064494
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC22050.029.3975264
GGTATCA17000.028.8259981
ATCAACG22550.028.5456943
GTACATG51700.027.7977261
AACGCAG23750.027.6781046
CAACGCA23750.027.4903935
GTATCAA24750.027.0341761
TACATGG49950.026.729552
ACATGGG49200.025.7848873
ACGCAGA27500.023.5623367
TATCAAC29000.022.3712312
CATGGGG36800.021.8267254
CGCAGAG29300.021.7948428
GAGTACA34950.018.8747331
GGACGCG1250.006448368518.7820269
TGTACCG2302.2921868E-618.3799555
TATACTG12650.017.0803625
TAGACAG10600.016.3955355
GTGTAGG8450.016.1711641
GCAGAGT39000.016.133289
Produced by FastQC (version 0.11.5)