##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033121_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2064494
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.38494226672492	33.0	31.0	34.0	30.0	34.0
2	31.449091157445842	33.0	31.0	34.0	28.0	34.0
3	31.650788522514475	34.0	31.0	34.0	30.0	34.0
4	35.029409627734445	37.0	35.0	37.0	33.0	37.0
5	35.149824605932494	37.0	35.0	37.0	33.0	37.0
6	35.192378858935896	37.0	35.0	37.0	33.0	37.0
7	35.13897109897147	37.0	35.0	37.0	33.0	37.0
8	35.16637490833105	37.0	35.0	37.0	33.0	37.0
9	36.79823239980354	39.0	38.0	39.0	33.0	39.0
10-11	36.787936172253346	39.0	38.0	39.0	33.0	39.0
12-13	36.73260203226553	39.0	38.0	39.0	33.0	39.0
14-15	38.14675460427591	41.0	39.0	41.0	33.0	41.0
16-17	38.14633827950093	41.0	39.0	41.0	33.0	41.0
18-19	38.12288919221853	40.5	38.5	41.0	33.5	41.0
20-21	38.15652624807822	40.5	39.0	41.0	33.5	41.0
22-23	38.10483779560512	40.0	39.0	41.0	33.0	41.0
24-25	38.10085667480748	40.0	38.5	41.0	33.0	41.0
26-27	38.06968826259606	40.0	38.0	41.0	33.0	41.0
28-29	38.03424810147184	40.0	38.0	41.0	33.0	41.0
30-31	38.02801921439345	40.0	38.0	41.0	33.0	41.0
32-33	38.10860118750648	40.0	38.0	41.0	33.0	41.0
34-35	38.08133809059265	40.5	38.0	41.0	33.0	41.0
36-37	37.995638882941776	40.0	38.0	41.0	33.0	41.0
38-39	37.90849186289716	40.0	38.0	41.0	33.0	41.0
40-41	37.841636255663616	40.0	38.0	41.0	33.0	41.0
42-43	37.713283981450175	40.0	38.0	41.0	32.0	41.0
44-45	37.56372215177181	40.0	38.0	41.0	32.0	41.0
46-47	37.429084317997535	40.0	38.0	41.0	32.0	41.0
48-49	37.30696093086247	40.0	37.5	41.0	31.0	41.0
50-51	37.18903033866894	40.0	37.0	41.0	31.0	41.0
52-53	37.04213526413736	40.0	37.0	41.0	31.0	41.0
54-55	36.890894572713705	40.0	36.5	41.0	30.5	41.0
56-57	36.7373395127329	40.0	36.0	41.0	30.0	41.0
58-59	36.544244982063404	39.5	36.0	41.0	30.0	41.0
60-61	36.32499561635926	39.0	35.0	41.0	30.0	41.0
62-63	36.07185102015312	39.0	35.0	41.0	30.0	41.0
64-65	35.80260877483781	39.0	35.0	40.5	29.5	41.0
66-67	35.5083882539741	38.0	35.0	40.0	29.0	41.0
68-69	35.200534368227764	37.5	35.0	40.0	29.0	41.0
70-71	34.84677213883886	37.0	34.0	40.0	28.5	41.0
72-73	34.483990023705566	36.5	34.0	39.0	28.0	41.0
74-75	34.11063267803152	36.0	34.0	39.0	28.0	40.5
76-77	33.78139631309173	35.5	34.0	38.5	28.0	40.0
78-79	33.406569115725205	35.0	34.0	37.0	27.0	39.5
80-81	33.036736362517885	35.0	33.5	37.0	26.5	39.0
82-83	32.81460614562212	35.0	33.5	36.5	27.0	39.0
84-85	32.533804893596205	35.0	33.0	36.0	26.0	37.5
86-87	32.26586466223685	35.0	33.0	36.0	26.0	37.0
88-89	31.89499799950981	35.0	33.0	35.5	25.5	37.0
90-91	31.690313461797416	35.0	33.0	35.0	25.0	36.0
92-93	31.49527511341762	35.0	33.0	35.0	25.0	36.0
94-95	31.308264640149112	35.0	33.0	35.0	24.0	36.0
96-97	31.108458295349855	35.0	33.0	35.0	23.5	36.0
98-99	30.899168028582306	35.0	33.0	35.0	21.5	35.0
100	30.724754830965843	35.0	33.0	35.0	19.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.1242689978154985
1101	2	0.10564703130120634
1101	3	0.18768122900199913
1101	4	0.08272596330982651
1101	5	0.012329225369782648
1101	6	0.10910794837253235
1101	7	0.18593586780223603
1101	8	0.05918622951686103
1101	9	0.21584163611868945
1101	10-11	0.11182374996256783
1101	12-13	0.2015395511790956
1101	14-15	0.23306929167519996
1101	16-17	0.12333855533680804
1101	18-19	0.1411726446094761
1101	20-21	0.0638289501187046
1101	22-23	0.1227027304550603
1101	24-25	0.14068785334013256
1101	26-27	0.1495445209980275
1101	28-29	0.16352904155795756
1101	30-31	0.1345947377214145
1101	32-33	0.15196944003604784
1101	34-35	0.16774552368412543
1101	36-37	0.22542447285261602
1101	38-39	0.16431946429768374
1101	40-41	0.12236274072392916
1101	42-43	0.16044391347073628
1101	44-45	0.13015610783966736
1101	46-47	0.07259528844775076
1101	48-49	0.022355558005486387
1101	50-51	-0.024473294932214174
1101	52-53	-0.029317173202272784
1101	54-55	-0.097111602148658
1101	56-57	0.006596343342380351
1101	58-59	-0.004986523731353998
1101	60-61	0.07819418664485056
1101	62-63	0.09050135314080165
1101	64-65	0.0336560411110014
1101	66-67	0.09973566771975584
1101	68-69	0.030692375934314953
1101	70-71	0.0682322433258662
1101	72-73	0.007621878616674849
1101	74-75	0.04872004746778913
1101	76-77	-0.032275460560022395
1101	78-79	0.02938341495885055
1101	80-81	-0.012479335715184448
1101	82-83	-0.07499508470344551
1101	84-85	-0.19025356759750167
1101	86-87	-0.019246735169012652
1101	88-89	0.20907409556709666
1101	90-91	0.23502545042456902
1101	92-93	0.23485067694532447
1101	94-95	0.2101925973344123
1101	96-97	0.1683798912896748
1101	98-99	0.22999142136379547
1101	100	0.17481983554759495
1102	1	0.16883965931150868
1102	2	0.18243486037123446
1102	3	0.17961381159578238
1102	4	0.17886855410705493
1102	5	0.09033176098019169
1102	6	0.08989458421412877
1102	7	0.0680132784965437
1102	8	0.13166531073097332
1102	9	0.05929483919125289
1102	10-11	0.07956004647983406
1102	12-13	0.1163302141666378
1102	14-15	0.12196570453223643
1102	16-17	0.10611281143346218
1102	18-19	0.11320993299786863
1102	20-21	0.19183730271770827
1102	22-23	0.24254671583800302
1102	24-25	0.26800950297843684
1102	26-27	0.27372440018365296
1102	28-29	0.2812890534902479
1102	30-31	0.2660491340224027
1102	32-33	0.21955181008600988
1102	34-35	0.32397641338507555
1102	36-37	0.24723609191443785
1102	38-39	0.21719529396433046
1102	40-41	0.23827712658417255
1102	42-43	0.14439707925490808
1102	44-45	0.14644158774122928
1102	46-47	0.15528780839704126
1102	48-49	0.06399087756697952
1102	50-51	0.1393143104074781
1102	52-53	0.02865642661873835
1102	54-55	0.10925188506141836
1102	56-57	0.10460872308733116
1102	58-59	-0.0397142439192919
1102	60-61	-0.08971811106563621
1102	62-63	-0.009296991254750253
1102	64-65	0.04748943659164695
1102	66-67	-0.049766308564834105
1102	68-69	-0.044477435386440334
1102	70-71	0.03241391235413005
1102	72-73	-0.008720279635255679
1102	74-75	0.028030958050244692
1102	76-77	-0.11296276013493411
1102	78-79	-0.05261643588759313
1102	80-81	-0.10889189444439751
1102	82-83	-0.17207764119535085
1102	84-85	-0.1859406434420876
1102	86-87	-0.18437105465592651
1102	88-89	-0.02419281471704693
1102	90-91	0.017774350417113283
1102	92-93	0.01776603230366547
1102	94-95	-0.008133004873059235
1102	96-97	0.04257960789792392
1102	98-99	-0.015011785437604175
1102	100	0.003714458561987044
1103	1	0.08441102485436147
1103	2	0.024024816216673628
1103	3	0.050909891366586635
1103	4	0.06519181433327503
1103	5	0.109020555997283
1103	6	0.023171778781033936
1103	7	-0.044694318783449205
1103	8	0.010676170241595173
1103	9	0.04194646281884218
1103	10-11	0.1560395352868298
1103	12-13	0.1629047510506183
1103	14-15	0.13986247063977686
1103	16-17	0.11575424138230517
1103	18-19	0.17137190293866666
1103	20-21	0.17252495268827062
1103	22-23	0.2420534473061693
1103	24-25	0.17900793977106133
1103	26-27	0.20571802524657556
1103	28-29	0.1705165733125682
1103	30-31	0.20493158474450723
1103	32-33	0.23590806239713658
1103	34-35	0.26261660728268765
1103	36-37	0.20351236376276205
1103	38-39	0.2052295002132425
1103	40-41	0.25174248201513194
1103	42-43	0.14152616885076696
1103	44-45	0.12472573437629819
1103	46-47	0.22189864348614918
1103	48-49	0.14088138508523684
1103	50-51	0.15782950531306028
1103	52-53	0.0883732522364511
1103	54-55	0.11813860954871558
1103	56-57	0.10349633458249485
1103	58-59	0.14539272116600443
1103	60-61	0.15934909072404224
1103	62-63	0.13518357348127097
1103	64-65	0.15406325926149833
1103	66-67	0.15941312851923328
1103	68-69	0.13546604954076003
1103	70-71	0.19625209907965058
1103	72-73	0.2048083183048135
1103	74-75	0.25541669242939946
1103	76-77	0.27608620615531265
1103	78-79	0.19699552696867073
1103	80-81	0.22210856979478422
1103	82-83	0.15823147528720938
1103	84-85	0.07027307707209474
1103	86-87	0.0913581061309543
1103	88-89	0.1963077802694002
1103	90-91	0.19875482818028445
1103	92-93	0.26700666141111995
1103	94-95	0.33404946070930563
1103	96-97	0.2554693297806381
1103	98-99	0.3067460568063183
1103	100	0.345844567640917
1104	1	0.01681505723961152
1104	2	-0.04045839487752545
1104	3	0.04238032272690617
1104	4	0.006663441800135672
1104	5	-0.01801171761518816
1104	6	0.011440631635117882
1104	7	-0.03174934500033544
1104	8	-0.0332410963980152
1104	9	-0.022717271762459745
1104	10-11	-0.02224324556341628
1104	12-13	-0.00812386513385377
1104	14-15	-0.12274050527329905
1104	16-17	-0.027238191250312127
1104	18-19	-0.055317088096735745
1104	20-21	-0.07049111412357689
1104	22-23	-0.11391749439293619
1104	24-25	-0.057428987316058056
1104	26-27	-0.09728477807275482
1104	28-29	-0.010382912095820984
1104	30-31	-0.006642385141958584
1104	32-33	-0.10485848241433615
1104	34-35	-0.10190860044114203
1104	36-37	-0.02044297007500262
1104	38-39	-0.12368030626557669
1104	40-41	-0.17315343456573373
1104	42-43	-0.18090184768562523
1104	44-45	-0.17080339657699284
1104	46-47	-0.2629287450500115
1104	48-49	-0.37229638647082197
1104	50-51	-0.4002259621342361
1104	52-53	-0.30670084877873904
1104	54-55	-0.3264793318721857
1104	56-57	-0.3242013508184485
1104	58-59	-0.33969393453782004
1104	60-61	-0.2874712372395152
1104	62-63	-0.4250800395401626
1104	64-65	-0.4425030732632109
1104	66-67	-0.41012850573441995
1104	68-69	-0.35281553963297085
1104	70-71	-0.2839811328356632
1104	72-73	-0.31035775887329464
1104	74-75	-0.4193865258946019
1104	76-77	-0.42891914531395514
1104	78-79	-0.3625496468743492
1104	80-81	-0.30506829980834027
1104	82-83	-0.270829660819075
1104	84-85	-0.33343602715928
1104	86-87	-0.32039259957190325
1104	88-89	-0.20964513123112027
1104	90-91	-0.24909437290914482
1104	92-93	-0.3322574451150153
1104	94-95	-0.2735046608966307
1104	96-97	-0.299282779353895
1104	98-99	-0.2628428679559214
1104	100	-0.3180333249434
1105	1	0.008382998767373806
1105	2	0.04318917973761316
1105	3	0.042620728988001844
1105	4	0.04267489805044278
1105	5	-0.05761461404341617
1105	6	-0.014633253788787215
1105	7	-0.06570566751510398
1105	8	-0.022068788820718055
1105	9	-0.004032268525897109
1105	10-11	0.050432501607353686
1105	12-13	0.05946384917176317
1105	14-15	0.04802609337736641
1105	16-17	0.09504884957797088
1105	18-19	0.02536363539868347
1105	20-21	0.09324253952490125
1105	22-23	0.11437405384037191
1105	24-25	0.15037026326201897
1105	26-27	0.22920794942547928
1105	28-29	0.22388778620366878
1105	30-31	0.20338731310146585
1105	32-33	0.17012062008028295
1105	34-35	0.16058126936570005
1105	36-37	0.14818832402425386
1105	38-39	0.09077014880733714
1105	40-41	0.10132032632262877
1105	42-43	0.12205952421012256
1105	44-45	0.05486290254276582
1105	46-47	0.04387063210239006
1105	48-49	0.05504846654156381
1105	50-51	0.1286202407210837
1105	52-53	0.06258875921464835
1105	54-55	-0.030829007451153245
1105	56-57	0.06014677868492413
1105	58-59	0.061140075557538864
1105	60-61	0.10369109305320734
1105	62-63	0.13436635496056226
1105	64-65	0.09850169748436599
1105	66-67	0.08623439388789222
1105	68-69	0.11225451373212536
1105	70-71	0.01502224304590527
1105	72-73	0.014377050933923385
1105	74-75	0.06818391823685488
1105	76-77	0.12382560609918158
1105	78-79	0.029594059153822627
1105	80-81	0.023858859643027586
1105	82-83	0.07734316664738117
1105	84-85	0.13691298241788985
1105	86-87	0.06214462517142749
1105	88-89	-0.0019149018852431254
1105	90-91	0.07554954124458035
1105	92-93	0.03777886642431483
1105	94-95	0.07361968095937499
1105	96-97	0.07996627388717314
1105	98-99	0.08101698198979435
1105	100	0.10580524947295444
1106	1	0.09845598599282468
1106	2	0.04602034701751734
1106	3	0.059486450797560764
1106	4	0.056868138186302986
1106	5	0.002826052929414402
1106	6	0.04445160417995453
1106	7	0.0664408157081482
1106	8	0.06427116283367695
1106	9	0.0545973238019144
1106	10-11	0.04821319626505982
1106	12-13	0.08615967036926264
1106	14-15	0.09818312791946227
1106	16-17	0.08406580804474117
1106	18-19	0.0645248887153329
1106	20-21	0.005147364036155011
1106	22-23	-0.0800509753131422
1106	24-25	0.03527871175707986
1106	26-27	0.09382716410193126
1106	28-29	0.12952147174362239
1106	30-31	0.08525821130276512
1106	32-33	0.08801518924121865
1106	34-35	0.10249785312245052
1106	36-37	0.0966255408845953
1106	38-39	0.07919588363787966
1106	40-41	0.06706742162184298
1106	42-43	0.11313042681425145
1106	44-45	0.05877352942327008
1106	46-47	0.04611404094234928
1106	48-49	0.004408546705818139
1106	50-51	0.021895864267861498
1106	52-53	0.032395586752031136
1106	54-55	-0.014901436576174376
1106	56-57	-0.02283916872114844
1106	58-59	-0.054920762689746994
1106	60-61	-0.069414825563193
1106	62-63	-0.04132088009205859
1106	64-65	-0.0424905655476806
1106	66-67	0.00652020327723335
1106	68-69	-0.03918796594602725
1106	70-71	-0.0701071320956359
1106	72-73	0.05292878641633081
1106	74-75	0.08488917088329373
1106	76-77	-0.008484607648398423
1106	78-79	0.050937731817519705
1106	80-81	0.010042816314218328
1106	82-83	0.002586703699606119
1106	84-85	0.03420430982655631
1106	86-87	0.04045713369427517
1106	88-89	0.05213879683469358
1106	90-91	0.012886358527023134
1106	92-93	-0.009401411635263202
1106	94-95	0.02371718546769941
1106	96-97	0.08635217200591683
1106	98-99	0.11526876559766563
1106	100	0.16624673178608518
1107	1	-0.03743141276720152
1107	2	-0.0531928958271628
1107	3	-0.006079219104151434
1107	4	0.009770801116069094
1107	5	-0.13228539790019767
1107	6	-0.09444307536513463
1107	7	-0.186143747174512
1107	8	-0.1310708333213242
1107	9	-0.10739049265744427
1107	10-11	-0.09927481707661201
1107	12-13	-0.1264539230031403
1107	14-15	-0.17893451505183577
1107	16-17	-0.04473768796742661
1107	18-19	-0.07470564888918574
1107	20-21	-0.09392883327853241
1107	22-23	-0.11382787291117324
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2216	8	0.055128546676428414
2216	9	-0.12307785525409543
2216	10-11	-0.0108794187513368
2216	12-13	0.0026830738667982246
2216	14-15	0.02177579840525823
2216	16-17	-0.02366455454155414
2216	18-19	-0.08446552884729641
2216	20-21	-0.03163029126309169
2216	22-23	-0.017517290437560007
2216	24-25	-0.04167623056655145
2216	26-27	-0.13936514844231596
2216	28-29	-0.09965278426484048
2216	30-31	-0.1183780898664466
2216	32-33	-0.14194835831415276
2216	34-35	-0.16479292954162617
2216	36-37	-0.11071658834836029
2216	38-39	-0.07947886189132447
2216	40-41	-0.09370834221016366
2216	42-43	-0.06943930399261689
2216	44-45	-0.10022747584488201
2216	46-47	0.014989150340333879
2216	48-49	0.031163550032673015
2216	50-51	-0.06783225174212504
2216	52-53	-0.005188407427752395
2216	54-55	0.04206249100360537
2216	56-57	-0.0459668928522774
2216	58-59	-0.09196907719364589
2216	60-61	-0.04550371088525651
2216	62-63	0.014434784933605727
2216	64-65	-0.02868196304351045
2216	66-67	0.04123316420762535
2216	68-69	-0.008685696421387945
2216	70-71	-0.0484633127900338
2216	72-73	0.09118654367436818
2216	74-75	0.03495805522447881
2216	76-77	-0.11944609562360142
2216	78-79	-0.11425132442373354
2216	80-81	-0.08403958210399765
2216	82-83	-0.0313866652180792
2216	84-85	-0.03852171355766387
2216	86-87	-0.07870333495527859
2216	88-89	-0.030064888379211396
2216	90-91	-0.10601341705138623
2216	92-93	-0.07840600208880844
2216	94-95	-0.0620986986083345
2216	96-97	-0.1294248467721104
2216	98-99	-0.027903659264904235
2216	100	-0.00739531040055752
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	26683.0
3	4189.0
4	2844.0
5	2218.0
6	1974.0
7	2090.0
8	2502.0
9	2802.0
10	2972.0
11	3289.0
12	3814.0
13	4088.0
14	4693.0
15	5011.0
16	4928.0
17	5115.0
18	4892.0
19	4811.0
20	4945.0
21	5085.0
22	5844.0
23	7536.0
24	11226.0
25	15079.0
26	15573.0
27	14090.0
28	15875.0
29	19745.0
30	24100.0
31	29439.0
32	38181.0
33	51791.0
34	72007.0
35	109569.0
36	200395.0
37	416787.0
38	650046.0
39	267715.0
40	551.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.16679237507241	19.844173415393705	13.137079184631952	23.851955024901926
2	15.316190006925357	19.79191925462224	40.911683288166564	23.98020745028584
3	18.336505739170562	25.07640190528539	29.809563555479297	26.777528800064758
4	11.878737894093122	16.449641462603857	39.469399543142245	32.202221100160784
5	12.597354515815143	38.04676505820918	36.149296954049056	13.206583471926622
6	31.04345298648452	38.42518044857821	17.73996027709151	12.791406287845758
7	27.370856381358	32.295459730888524	22.631105496212122	17.70257839154135
8	24.906076095439765	37.160099623529256	20.206465452716856	17.727358828314127
9	24.518657327425412	17.11395934930203	21.233289505174596	37.134093818097966
10-11	23.654803054083064	27.15669362733045	29.12194990786347	20.066553410723014
12-13	24.2470625909273	24.3413387308765	29.206612507139745	22.20498617105645
14-15	21.42627364288244	26.574645886655627	26.082082303222858	25.91699816723907
16-17	21.302779073077087	29.986858599744437	26.623677533050316	22.086684794128157
18-19	21.144323438914817	29.023861812428027	28.54173721823581	21.290077530421353
20-21	22.298659449622892	28.362894821373807	28.013936995246613	21.324508733756687
22-23	21.83208089076148	28.127866623162422	28.1183712651087	21.921681220967397
24-25	21.785680284246045	28.205756190750282	28.10260629127177	21.905957233731908
26-27	21.56281755783716	28.128172550406532	28.19338280624536	22.115627085510944
28-29	21.620441863816993	28.220735696836023	28.178852985384655	21.979969453962326
30-31	21.866308861137266	28.750278273900005	27.812831339733492	21.570581525229237
32-33	21.39854979777014	28.346022360595118	28.412955889278425	21.842471952356313
34-35	21.48674750275776	28.13421523444937	28.473091212623725	21.90594605016915
36-37	21.88902347957536	28.432456104018662	27.85671154180131	21.821808874604677
38-39	21.80005164195749	28.636264765312497	27.938274780751794	21.62540881197822
40-41	21.506754619382633	28.34384783928235	28.29467841501029	21.854719126324728
42-43	21.507691413374413	28.20521577375546	28.306435791049335	21.9806570218208
44-45	21.6473445771553	28.334804369899008	28.255407149334594	21.762443903611096
46-47	21.6877546378883	28.49023302250587	28.12805713819288	21.693955201412955
48-49	21.602255374133676	28.34984941163363	28.149258683808025	21.89863653042466
50-51	21.60423647243527	28.267927235524443	28.186982012334095	21.940854279706194
52-53	21.65577773697894	28.255500940014894	28.16036106647729	21.92836025652888
54-55	21.714430479323823	28.689870177177323	27.97451498695281	21.621184356546046
56-57	21.5075915315472	28.338964808106414	28.079233166790875	22.074210493555512
58-59	21.45875430110691	27.97238669382174	28.466612850606666	22.102246154464687
60-61	21.68892268903289	28.414992570561502	28.116458719084875	21.779626021320734
62-63	21.53816345090745	28.471148990587047	28.027155030958724	21.963532527546782
64-65	21.533806921596366	28.180511869617668	28.2093088648486	22.07637234393736
66-67	21.35901849417802	28.274747809415583	28.076875290178716	22.28935840622768
68-69	21.828657568234007	28.387419914004962	28.04434559512193	21.739576922639102
70-71	21.816685041338165	28.18082033058908	28.11305363789432	21.889440990178432
72-73	21.559708019155703	28.31562200207755	28.30690297490785	21.817767003858897
74-75	21.605122161398654	28.403257831054507	28.06285091218398	21.92876909536286
76-77	21.606956147699705	28.427666192683315	27.935130401127484	22.030247258489496
78-79	21.784778619257676	28.455252653471184	28.041479961548493	21.71848876572265
80-81	21.714520855439087	28.23441137155904	28.15000483188082	21.90106294112105
82-83	21.596344193733707	28.33467832954377	28.278270835412183	21.79070664131034
84-85	21.566811905551937	28.254687163191512	28.13983622479007	22.03866470646648
86-87	21.755290252749727	28.38873230840325	27.99753540135829	21.858442037488736
88-89	21.60542544220117	28.22714324386688	28.041741967914298	22.125689346017648
90-91	21.519240591712286	28.204758924811678	28.163332046663236	22.1126684368128
92-93	21.72892351171932	28.36760946434981	28.079484787070648	21.82398223686022
94-95	21.543235459150544	28.128271680467876	28.17563605062043	22.152856809761154
96-97	21.556499904736707	28.164556499417458	28.200834516206214	22.078109079639628
98-99	21.56125206752817	28.27969231136554	28.069510364014082	22.08954525709221
100	21.727433749058953	28.128649984041786	27.971699147514784	22.172217119384477
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	274.0
1	263.0
2	241.0
3	208.5
4	176.5
5	151.5
6	131.5
7	132.0
8	139.0
9	129.5
10	127.0
11	145.0
12	160.5
13	173.5
14	199.5
15	240.5
16	330.5
17	480.5
18	693.0
19	989.5
20	1464.5
21	2142.5
22	3259.0
23	4953.5
24	7277.0
25	10347.0
26	14201.5
27	19144.5
28	25053.5
29	31013.5
30	37021.0
31	43118.5
32	48890.5
33	54574.5
34	59708.5
35	63569.5
36	66842.5
37	70430.5
38	73203.5
39	74187.5
40	74590.0
41	74275.0
42	73419.5
43	72799.0
44	71433.5
45	70069.5
46	68737.5
47	67271.5
48	66009.0
49	64167.5
50	62304.0
51	60040.0
52	56032.5
53	53228.0
54	51769.5
55	49311.5
56	45468.5
57	41661.0
58	37600.5
59	32910.0
60	28914.5
61	24620.5
62	20839.5
63	17495.0
64	14164.5
65	11639.5
66	9142.0
67	6817.0
68	5390.0
69	4236.5
70	3219.0
71	2389.0
72	1890.5
73	1474.5
74	1086.0
75	767.0
76	496.0
77	323.5
78	225.5
79	177.5
80	131.0
81	84.0
82	52.5
83	32.5
84	25.5
85	20.5
86	17.0
87	14.5
88	11.0
89	9.0
90	7.0
91	6.0
92	4.0
93	5.5
94	3.5
95	2.0
96	2.5
97	1.5
98	0.5
99	2.0
100	3.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3304441669484145
2	0.12138567610271572
3	0.06737728470027038
4	0.04030043197025518
5	0.04369109331390646
6	0.00939697572383354
7	0.007023512783277647
8	0.015645480199990895
9	0.01801894314054679
10-11	0.021191633397820483
12-13	0.016711116622281294
14-15	0.01913301758203221
16-17	0.019690054802774918
18-19	0.019423645697202317
20-21	0.02378306742475396
22-23	0.015863451286368475
24-25	0.024582294741471764
26-27	0.019447864706799826
28-29	0.019205674610824737
30-31	0.020852567263455357
32-33	0.024509637712679236
34-35	0.015354852084820785
36-37	0.010196203040551341
38-39	0.013611083393800127
40-41	0.009832917896588704
42-43	0.008670405435908267
44-45	0.009203223647053466
46-47	0.00801649217677552
48-49	0.0044320787563441695
50-51	0.006539132591327464
52-53	0.00799227316717801
54-55	0.003148471247676186
56-57	0.0026883100653235124
58-59	0.0026398720461284944
60-61	0.0034633183724438045
62-63	0.00249455798854344
64-65	0.0020586158157882757
66-67	0.0016226736430331112
68-69	0.0035117563916388234
70-71	0.002809405113311058
72-73	0.0021070538349832937
74-75	0.002567215017335967
76-77	0.0036570704492238774
78-79	0.0036570704492238774
80-81	0.0038992605451989687
82-83	0.0025187769981409487
84-85	0.003826603516406441
86-87	0.0029305001612986038
88-89	0.6034650621411348
90-91	0.6144847115080015
92-93	0.6105370129436075
94-95	0.5966837394538324
96-97	0.5951579418491891
98-99	0.5921305656495006
100	0.5938016773117287
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2064494.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	72.51550732127389
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.04718888989187	59.49693526634895
2	11.807932728595699	17.12516464459182
3	3.11725705667294	6.781484307463778
4	1.19971644132173	3.4799218553647426
5	0.5864618697565904	2.1263790004991
6	0.3254330944046256	1.4159367563930068
7	0.20419389314519676	1.0365056627330955
8	0.1332659441263999	0.7731078037579543
9	0.0991203439560543	0.6468985825049198
>10	0.45616368645746375	5.72506010645287
>50	0.01877991875461551	0.9159214160684308
>100	0.004486132916737777	0.476684597821283
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	4.843801919501825E-5	0.0
6	0.0	0.0	0.0	4.843801919501825E-5	0.0
7	0.0	0.0	0.0	4.843801919501825E-5	0.0
8	0.0	0.0	0.0	4.843801919501825E-5	0.0
9	0.0	0.0	0.0	4.843801919501825E-5	0.0
10-11	0.0	0.0	0.0	4.843801919501825E-5	0.0
12-13	0.0	0.0	0.0	4.843801919501825E-5	0.0
14-15	2.4219009597509124E-5	0.0	0.0	9.68760383900365E-5	0.0
16-17	4.843801919501825E-5	0.0	0.0	9.68760383900365E-5	0.0
18-19	4.843801919501825E-5	0.0	0.0	1.2109504798754562E-4	0.0
20-21	4.843801919501825E-5	0.0	0.0	1.93752076780073E-4	0.0
22-23	4.843801919501825E-5	0.0	0.0	1.93752076780073E-4	0.0
24-25	4.843801919501825E-5	0.0	0.0	2.4219009597509122E-4	0.0
26-27	4.843801919501825E-5	0.0	0.0	3.8750415356014593E-4	0.0
28-29	4.843801919501825E-5	0.0	0.0	0.0010656364222904014	0.0
30-31	4.843801919501825E-5	0.0	0.0	0.004359421727551643	0.0
32-33	4.843801919501825E-5	0.0	0.0	0.010995430357269142	0.0
34-35	4.843801919501825E-5	0.0	0.0	0.01985958786995748	0.0
36-37	4.843801919501825E-5	0.0	0.0	0.03780587398171174	0.0
38-39	4.843801919501825E-5	0.0	0.0	0.06914527240088855	0.0
40-41	4.843801919501825E-5	0.0	0.0	0.11215823344606475	0.0
42-43	4.843801919501825E-5	0.0	0.0	0.15330633075223277	0.0
44-45	4.843801919501825E-5	0.0	0.0	0.19716695713332177	0.0
46-47	4.843801919501825E-5	0.0	0.0	0.2519503568428874	0.0
48-49	4.843801919501825E-5	0.0	0.0	0.3139025833933158	0.0
50-51	4.843801919501825E-5	0.0	0.0	0.383604893014947	0.0
52-53	4.843801919501825E-5	0.0	0.0	0.45323454560778575	0.0
54-55	4.843801919501825E-5	0.0	0.0	0.5237845205653298	0.0
56-57	4.843801919501825E-5	0.0	0.0	0.6069041615039812	0.0
58-59	4.843801919501825E-5	0.0	0.0	0.7078732125159967	0.0
60-61	4.843801919501825E-5	0.0	0.0	0.8177064210407006	0.0
62-63	4.843801919501825E-5	0.0	0.0	0.9276607246133919	0.0
64-65	4.843801919501825E-5	0.0	0.0	1.040497090328187	0.0
66-67	4.843801919501825E-5	0.0	0.0	1.1691000312909603	0.0
68-69	4.843801919501825E-5	0.0	0.0	1.3171992749797288	0.0
70-71	4.843801919501825E-5	0.0	0.0	1.4765119201121437	0.0
72-73	4.843801919501825E-5	0.0	0.0	1.648248917168081	0.0
74-75	4.843801919501825E-5	0.0	0.0	1.8185085546385702	0.0
76-77	4.843801919501825E-5	0.0	0.0	1.9994487753415608	0.0
78-79	4.843801919501825E-5	0.0	0.0	2.2087010182640396	0.0
80-81	4.843801919501825E-5	0.0	0.0	2.442898841071953	0.0
82-83	4.843801919501825E-5	0.0	0.0	2.6823279699529277	0.0
84-85	4.843801919501825E-5	0.0	0.0	2.920957871517185	0.0
86-87	4.843801919501825E-5	0.0	0.0	3.169493348006824	0.0
88	4.843801919501825E-5	0.0	0.0	3.3695908053014443	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCAACGC	2205	0.0	29.397526	4
GGTATCA	1700	0.0	28.825998	1
ATCAACG	2255	0.0	28.545694	3
GTACATG	5170	0.0	27.797726	1
AACGCAG	2375	0.0	27.678104	6
CAACGCA	2375	0.0	27.490393	5
GTATCAA	2475	0.0	27.034176	1
TACATGG	4995	0.0	26.72955	2
ACATGGG	4920	0.0	25.784887	3
ACGCAGA	2750	0.0	23.562336	7
TATCAAC	2900	0.0	22.371231	2
CATGGGG	3680	0.0	21.826725	4
CGCAGAG	2930	0.0	21.794842	8
GAGTACA	3495	0.0	18.874733	1
GGACGCG	125	0.0064483685	18.782026	9
TGTACCG	230	2.2921868E-6	18.379955	5
TATACTG	1265	0.0	17.080362	5
TAGACAG	1060	0.0	16.395535	5
GTGTAGG	845	0.0	16.171164	1
GCAGAGT	3900	0.0	16.13328	9
>>END_MODULE