FastQCFastQC Report
Wed 25 May 2016
SRR1033121_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033121_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2064494
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34680.16798305056832327No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30190.14623437994976007No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28600.044.590661
GTATCAA48450.040.8911131
ATCAACG53700.036.2421573
TCAACGC53700.036.2421574
CAACGCA54850.035.5685
AACGCAG56950.034.2456636
TATCAAC59650.032.9422872
ACGCAGA65500.029.4166897
CGCAGAG66350.029.039138
GTACATG50100.026.8638941
TACATGG50700.024.8493372
GTATAGG6650.024.7677761
GCAGAGT78350.024.591539
ACATGGG51100.023.7348653
TCCGTAC1100.003056678721.3680693
GAGTACT52850.021.20723512-13
AGAGTAC70050.020.49480410-11
CAGAGTA78050.019.8692610-11
CATGGGG34300.019.0505984
GTACTTT60900.018.4039814-15
Produced by FastQC (version 0.11.5)