##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033121_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2064494
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.746328640335115	34.0	31.0	34.0	31.0	34.0
2	32.8451213711447	34.0	31.0	34.0	31.0	34.0
3	32.93226136767653	34.0	31.0	34.0	31.0	34.0
4	36.364278607736324	37.0	37.0	37.0	35.0	37.0
5	36.300083216516974	37.0	37.0	37.0	35.0	37.0
6	36.3867548174032	37.0	37.0	37.0	35.0	37.0
7	36.3592076315068	37.0	37.0	37.0	35.0	37.0
8	36.36011390684594	37.0	37.0	37.0	35.0	37.0
9	38.17619862300399	39.0	39.0	39.0	37.0	39.0
10-11	38.15253834595789	39.0	39.0	39.0	37.0	39.0
12-13	38.11457141556237	39.0	39.0	39.0	37.0	39.0
14-15	39.65267421460173	41.0	40.0	41.0	37.0	41.0
16-17	39.66638847097642	41.0	40.0	41.0	37.0	41.0
18-19	39.6805602244424	41.0	40.0	41.0	37.0	41.0
20-21	39.67152895576349	41.0	40.0	41.0	37.0	41.0
22-23	39.60283948512323	41.0	40.0	41.0	37.0	41.0
24-25	39.55487543194604	41.0	40.0	41.0	37.0	41.0
26-27	39.456595901949825	41.0	39.0	41.0	36.5	41.0
28-29	39.366698328985215	41.0	39.0	41.0	36.0	41.0
30-31	39.27994486784655	41.0	39.0	41.0	36.0	41.0
32-33	39.323446326315306	41.0	39.0	41.0	36.0	41.0
34-35	39.2726249143858	41.0	39.0	41.0	35.0	41.0
36-37	39.18966148605905	41.0	39.0	41.0	35.0	41.0
38-39	39.102980924139274	41.0	39.0	41.0	35.0	41.0
40-41	38.998070229315275	41.0	39.0	41.0	35.0	41.0
42-43	38.889007427485865	41.0	39.0	41.0	35.0	41.0
44-45	38.752860991603754	41.0	39.0	41.0	35.0	41.0
46-47	38.5600704095047	41.0	39.0	41.0	35.0	41.0
48-49	38.385098479336826	41.0	38.0	41.0	35.0	41.0
50-51	38.26436381021209	41.0	38.0	41.0	34.0	41.0
52-53	38.13225444103979	40.0	38.0	41.0	34.0	41.0
54-55	37.99914894400274	40.0	38.0	41.0	34.0	41.0
56-57	37.85107997407597	40.0	37.5	41.0	34.0	41.0
58-59	37.66897142834999	40.0	37.0	41.0	33.5	41.0
60-61	37.48085632605374	40.0	36.5	41.0	33.0	41.0
62-63	37.25226447739737	39.5	36.0	41.0	33.0	41.0
64-65	37.00612789380836	39.0	35.5	41.0	33.0	41.0
66-67	36.73274129157072	39.0	35.0	41.0	32.5	41.0
68-69	36.4127437522221	38.5	35.0	41.0	32.0	41.0
70-71	36.078350917948896	37.0	35.0	40.0	32.0	41.0
72-73	35.72919901922698	37.0	35.0	39.5	31.5	41.0
74-75	35.38971341161563	36.5	35.0	39.0	31.0	41.0
76-77	35.05015006098347	36.0	35.0	39.0	31.0	41.0
78-79	34.70446414472505	35.5	35.0	38.0	31.0	39.5
80-81	34.35601605042204	35.0	35.0	37.0	31.0	39.0
82-83	34.02935779905391	35.0	34.5	37.0	30.5	39.0
84-85	33.75016057203363	35.0	34.0	36.0	30.0	38.0
86-87	33.50628531737075	35.0	34.0	36.0	30.0	37.0
88-89	33.28297999412931	35.0	34.0	36.0	30.0	37.0
90-91	33.07443664161775	35.0	34.0	35.0	30.0	36.5
92-93	32.903576372709246	35.0	34.0	35.0	30.0	36.0
94-95	32.74955727650456	35.0	34.0	35.0	29.0	36.0
96-97	32.61987973808594	35.0	34.0	35.0	29.0	36.0
98-99	32.51151202183198	35.0	34.0	35.0	29.0	36.0
100	32.40950760815967	35.0	34.0	35.0	29.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.13130110802734407
1101	2	0.13909848919641377
1101	3	0.16367423113165103
1101	4	0.08370204335509612
1101	5	0.03926962813826407
1101	6	0.05455547727532206
1101	7	0.09871923429993501
1101	8	0.12822941789222853
1101	9	0.20158825535497726
1101	10-11	0.13386341800595858
1101	12-13	0.16107249129115075
1101	14-15	0.24793427456158668
1101	16-17	0.25359487720464813
1101	18-19	0.2368633803115472
1101	20-21	0.23926055379218525
1101	22-23	0.2804163204471095
1101	24-25	0.20342537230447988
1101	26-27	0.23378332325646056
1101	28-29	0.1830062508418422
1101	30-31	0.162655047352132
1101	32-33	0.1442756000806824
1101	34-35	0.19034392495580477
1101	36-37	0.1454197345021342
1101	38-39	0.14289402808190488
1101	40-41	0.16483423847515866
1101	42-43	0.06443814004462922
1101	44-45	0.011064408897780709
1101	46-47	0.11559456048636463
1101	48-49	0.12094978143800716
1101	50-51	0.04818322430948996
1101	52-53	0.08387605261235365
1101	54-55	0.07255312368438638
1101	56-57	0.12602813642811128
1101	58-59	0.12516764988197338
1101	60-61	0.12720561574267464
1101	62-63	0.027931378193386536
1101	64-65	0.06693330270743303
1101	66-67	0.08525247483259335
1101	68-69	0.1897328997974057
1101	70-71	0.1612079205825907
1101	72-73	0.1715170427710575
1101	74-75	0.1303145143536213
1101	76-77	0.09173786402163131
1101	78-79	0.025863091245099668
1101	80-81	-0.008914859324683277
1101	82-83	0.046196194799357215
1101	84-85	-0.007671859817200755
1101	86-87	-0.08939772456901807
1101	88-89	0.04167066371149275
1101	90-91	0.06982682143590324
1101	92-93	0.014332110751887228
1101	94-95	0.06327728512633968
1101	96-97	0.02443293645333
1101	98-99	0.12465948597877485
1101	100	0.1198673107649526
1102	1	0.13153736725622167
1102	2	0.13061761813965944
1102	3	0.15439509025612352
1102	4	0.1138227085799457
1102	5	0.07435770331165514
1102	6	0.11257322654820001
1102	7	0.1781345523698903
1102	8	0.1722428417120554
1102	9	0.2123899557054898
1102	10-11	0.18317030144245194
1102	12-13	0.169908944419646
1102	14-15	0.24737882672804545
1102	16-17	0.19460792814058436
1102	18-19	0.26114080592121525
1102	20-21	0.21561792570657445
1102	22-23	0.2321579864922967
1102	24-25	0.24779437819604055
1102	26-27	0.25467854288506686
1102	28-29	0.22814011615618313
1102	30-31	0.24452215153588952
1102	32-33	0.18031587154014517
1102	34-35	0.24147137666734864
1102	36-37	0.2672358281705698
1102	38-39	0.28942397065778636
1102	40-41	0.2585098294961128
1102	42-43	0.25247721827567204
1102	44-45	0.23196022514060388
1102	46-47	0.32597922750105823
1102	48-49	0.2643835945486117
1102	50-51	0.217686692129611
1102	52-53	0.2303687067998368
1102	54-55	0.21375082671965373
1102	56-57	0.16136499972888174
1102	58-59	0.14360631048305805
1102	60-61	0.1311907003873145
1102	62-63	0.13170257664218354
1102	64-65	0.20247748944768773
1102	66-67	0.22954600902604483
1102	68-69	0.2575947836067911
1102	70-71	0.22879983645999147
1102	72-73	0.22271966787360498
1102	74-75	0.19840490140708766
1102	76-77	0.18364628373973346
1102	78-79	0.1520723536807722
1102	80-81	0.1202145313830485
1102	82-83	0.12340732165445445
1102	84-85	0.16299560233508004
1102	86-87	0.21136385347558218
1102	88-89	0.19117174507475454
1102	90-91	0.1388932693440239
1102	92-93	0.1529843592416995
1102	94-95	0.10733754316199651
1102	96-97	0.15389135990026404
1102	98-99	0.03276299854496045
1102	100	0.03606866806229192
1103	1	0.14542917763928642
1103	2	0.0778884562008173
1103	3	0.0946932400959426
1103	4	0.06024317104511567
1103	5	0.08717945965634755
1103	6	0.1243887473427705
1103	7	0.12637245939994557
1103	8	0.08466671166979012
1103	9	0.12605229600087142
1103	10-11	0.11377984864220991
1103	12-13	0.07076104795304872
1103	14-15	0.12694896197946548
1103	16-17	0.17890518175687475
1103	18-19	0.1539652578482702
1103	20-21	0.13111262842646454
1103	22-23	0.14194819185190255
1103	24-25	0.17506382238769902
1103	26-27	0.2111732260216641
1103	28-29	0.20591774673930274
1103	30-31	0.23552852532101554
1103	32-33	0.17238936187003873
1103	34-35	0.23993057488134184
1103	36-37	0.2188555390981577
1103	38-39	0.2173767564960727
1103	40-41	0.23338383406017016
1103	42-43	0.23617078815979653
1103	44-45	0.25735272612264737
1103	46-47	0.16314278368840007
1103	48-49	0.2697768903818556
1103	50-51	0.27997946923605355
1103	52-53	0.322254888714788
1103	54-55	0.2309556114935276
1103	56-57	0.21300527311161943
1103	58-59	0.26054890955434473
1103	60-61	0.23597829656143432
1103	62-63	0.22339211928019154
1103	64-65	0.23919206989872066
1103	66-67	0.28153699610452776
1103	68-69	0.25039602357467317
1103	70-71	0.2693479036468105
1103	72-73	0.2621427326125101
1103	74-75	0.19582658840390366
1103	76-77	0.23086683882234382
1103	78-79	0.19419082798115994
1103	80-81	0.16198384713135283
1103	82-83	0.20223006637935015
1103	84-85	0.2201492632008737
1103	86-87	0.18797097925905604
1103	88-89	0.10235276725341436
1103	90-91	0.15976012946287454
1103	92-93	0.14038268452476643
1103	94-95	0.13055710525657815
1103	96-97	0.22917390646529867
1103	98-99	0.21846857874602676
1103	100	0.24082832924832331
1104	1	0.030510583764410626
1104	2	0.059973216740289104
1104	3	0.09774707729432208
1104	4	0.05090911000647225
1104	5	0.060099386082505646
1104	6	0.05218998455993784
1104	7	0.06091264946570618
1104	8	0.1211958054108706
1104	9	0.1462159942559751
1104	10-11	0.11549304976000485
1104	12-13	0.16020711329413473
1104	14-15	0.16771326962870603
1104	16-17	0.14938006706354656
1104	18-19	0.1591480539377983
1104	20-21	0.13968793706624894
1104	22-23	0.12292400657765512
1104	24-25	0.10205414367077736
1104	26-27	0.12387073865166798
1104	28-29	0.1567672924846164
1104	30-31	0.1403627449545226
1104	32-33	0.12964837741233737
1104	34-35	0.07806435629686348
1104	36-37	0.15644170306930505
1104	38-39	0.15206834263967295
1104	40-41	0.23715533518274157
1104	42-43	0.22636116402077278
1104	44-45	0.24011350835860412
1104	46-47	0.26554999240101296
1104	48-49	0.29509392506540166
1104	50-51	0.29913234671398925
1104	52-53	0.2567907595069272
1104	54-55	0.25465103581011306
1104	56-57	0.25928252440562716
1104	58-59	0.26961063485386205
1104	60-61	0.23038251146368083
1104	62-63	0.26997382398895553
1104	64-65	0.2832053573133777
1104	66-67	0.30704219606695204
1104	68-69	0.28708061116599737
1104	70-71	0.23076826244332693
1104	72-73	0.2928159988339303
1104	74-75	0.367797249239473
1104	76-77	0.3000520776272282
1104	78-79	0.2591480516672604
1104	80-81	0.2334145544644599
1104	82-83	0.25630139154135634
1104	84-85	0.2587431912324689
1104	86-87	0.28120781632807734
1104	88-89	0.2886068229405865
1104	90-91	0.3054188829249469
1104	92-93	0.3095118170728668
1104	94-95	0.2437596184979114
1104	96-97	0.40947412034686437
1104	98-99	0.36441140751141887
1104	100	0.4231335720933913
1105	1	0.06790034092860253
1105	2	0.07522436058138737
1105	3	0.028973259659885287
1105	4	0.06757359631011184
1105	5	0.10423326093873442
1105	6	0.06735426783496479
1105	7	-0.05030285478430585
1105	8	0.027208000719298298
1105	9	0.03043956698184047
1105	10-11	-0.0015114245148311056
1105	12-13	0.012675503693515111
1105	14-15	0.015971789609608322
1105	16-17	0.046654705307346944
1105	18-19	0.019538595183185237
1105	20-21	0.04891832696613818
1105	22-23	0.16563466362580215
1105	24-25	0.04660418470124483
1105	26-27	0.03860551872666207
1105	28-29	0.06708195284917906
1105	30-31	0.1412041668683628
1105	32-33	0.14527574006220334
1105	34-35	0.10067422535615833
1105	36-37	0.04712364558587012
1105	38-39	0.06663545421366024
1105	40-41	0.15902181371522062
1105	42-43	0.15862627521078565
1105	44-45	0.25123272268035635
1105	46-47	0.18670427800365275
1105	48-49	0.058283245420760466
1105	50-51	0.0638038183910723
1105	52-53	0.023082637343172507
1105	54-55	0.06943489529052727
1105	56-57	0.0030533051058441174
1105	58-59	-0.0733638319624248
1105	60-61	-0.04333594477133573
1105	62-63	0.0033530865278805777
1105	64-65	-0.050245453696717846
1105	66-67	-0.06358674239073281
1105	68-69	-0.05155157065249227
1105	70-71	-0.06109141590498979
1105	72-73	-0.022687274259084234
1105	74-75	-0.00241501334872396
1105	76-77	-0.07540707284670134
1105	78-79	-0.08132943934086967
1105	80-81	0.024489939241902903
1105	82-83	0.0035993695802858383
1105	84-85	0.02672523976565344
1105	86-87	0.0706821208913837
1105	88-89	0.06287394487838327
1105	90-91	0.056778919758919244
1105	92-93	0.16514562736768568
1105	94-95	0.06860677363386003
1105	96-97	0.07002430239302271
1105	98-99	0.013440246918335674
1105	100	0.0017566133770117176
1106	1	0.09245645438075911
1106	2	0.06596637529135307
1106	3	0.04472971098906697
1106	4	0.04216749059349212
1106	5	0.04515767746592303
1106	6	0.08939699288978176
1106	7	0.05997885140496351
1106	8	0.05419454169310711
1106	9	0.09549689457762867
1106	10-11	0.03555063327770824
1106	12-13	0.07692676548359145
1106	14-15	0.07731198077021162
1106	16-17	0.10564508571862774
1106	18-19	0.14463727683907024
1106	20-21	0.07223945465515413
1106	22-23	0.10590583969507605
1106	24-25	0.08053304259089344
1106	26-27	0.0847038402059681
1106	28-29	0.049012279166717576
1106	30-31	0.16462165758927938
1106	32-33	0.09901047765613669
1106	34-35	0.10846969398012618
1106	36-37	0.13901479072521283
1106	38-39	0.16723937677507905
1106	40-41	0.1688725004457794
1106	42-43	0.12080116081344983
1106	44-45	0.13808886627973038
1106	46-47	0.12576431701361201
1106	48-49	0.10422863963474072
1106	50-51	0.046577818085729916
1106	52-53	0.05690081278687842
1106	54-55	-0.03054967439426548
1106	56-57	0.007493597429807153
1106	58-59	0.0172112520125296
1106	60-61	0.04310536387430375
1106	62-63	0.09116360633382214
1106	64-65	0.07370451539168954
1106	66-67	0.06751620716395479
1106	68-69	0.08723320947162705
1106	70-71	0.08671158850172134
1106	72-73	0.15335152533984342
1106	74-75	0.1025312587829248
1106	76-77	0.07726575534663027
1106	78-79	0.0675978174242644
1106	80-81	0.08713656035958195
1106	82-83	0.11002971393722305
1106	84-85	0.1010394825173293
1106	86-87	0.08116637799508197
1106	88-89	0.01925823270003235
1106	90-91	-0.09284056092230486
1106	92-93	-0.0988472938478111
1106	94-95	-0.036044294349828476
1106	96-97	0.02379547550668093
1106	98-99	-0.03131887811553469
1106	100	-0.026710279831959838
1107	1	-0.0032013255588623224
1107	2	0.030357700116276476
1107	3	-0.014778932530418842
1107	4	-0.020410200710486492
1107	5	-0.0741684445702262
1107	6	-0.10221968111171265
1107	7	-0.06276375778750776
1107	8	-0.027206642689108662
1107	9	-0.007688738418302421
1107	10-11	-0.017651985643070134
1107	12-13	-0.052897816238015594
1107	14-15	0.023356576543804408
1107	16-17	0.01723334690038314
1107	18-19	0.021215230652799733
1107	20-21	0.011738678707217787
1107	22-23	-0.006344100468140823
1107	24-25	0.03703556345656267
1107	26-27	0.07873946432288648
1107	28-29	0.07241811151563127
1107	30-31	0.09739599957771361
1107	32-33	0.11379981358368241
1107	34-35	0.10665721859041355
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2216	9	-0.15033614698403852
2216	10-11	-0.11455376197075395
2216	12-13	-0.06349487539833376
2216	14-15	-0.2202918460152432
2216	16-17	-0.15137218690206566
2216	18-19	-0.1234966931728394
2216	20-21	-0.11078793149291499
2216	22-23	-0.15228245361242188
2216	24-25	-0.26492317421762124
2216	26-27	-0.27957945185971056
2216	28-29	-0.28631401860089767
2216	30-31	-0.20921469797403347
2216	32-33	-0.2215845634580944
2216	34-35	-0.167891056973275
2216	36-37	-0.2116221107724101
2216	38-39	-0.15738526060845714
2216	40-41	-0.2419180158864762
2216	42-43	-0.24455178587062676
2216	44-45	-0.14643467254741438
2216	46-47	-0.2728727617762772
2216	48-49	-0.29070628282480726
2216	50-51	-0.2366928728531903
2216	52-53	-0.18672971327023902
2216	54-55	-0.1796071010913849
2216	56-57	-0.15051708888797322
2216	58-59	-0.1606130256460503
2216	60-61	-0.23763196215437432
2216	62-63	-0.19298266188682334
2216	64-65	-0.16146332085320125
2216	66-67	-0.2459041357602061
2216	68-69	-0.15689942612745256
2216	70-71	-0.09873612562130774
2216	72-73	-0.09533799367094531
2216	74-75	-0.09042095386918447
2216	76-77	-0.1416290896183625
2216	78-79	-0.1282490324776333
2216	80-81	-0.12685547145441234
2216	82-83	-0.10678955259206901
2216	84-85	-0.1310924858753424
2216	86-87	-0.12620302697167318
2216	88-89	-0.11397310512488446
2216	90-91	-0.14625156190763988
2216	92-93	-0.07602419245525738
2216	94-95	-0.11226816832753883
2216	96-97	-0.2052826597667874
2216	98-99	-0.153752434821385
2216	100	-0.07172127273948092
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	7.0
3	5.0
4	6.0
5	17.0
6	39.0
7	95.0
8	214.0
9	425.0
10	723.0
11	1306.0
12	1839.0
13	2903.0
14	3670.0
15	4776.0
16	6059.0
17	8296.0
18	10071.0
19	5669.0
20	3875.0
21	3527.0
22	3643.0
23	3851.0
24	4528.0
25	5701.0
26	6990.0
27	9057.0
28	11734.0
29	15084.0
30	19548.0
31	24586.0
32	31830.0
33	41417.0
34	58036.0
35	88892.0
36	165202.0
37	387396.0
38	741309.0
39	390282.0
40	1886.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.0757542553831	19.842632761940962	13.393426822149518	23.68818616052641
2	15.635532425459855	19.97909378635942	40.42717265258	23.958201135600724
3	18.419477121270393	25.086486083272707	30.099821069957095	26.39421572549981
4	12.058922115313822	16.67532095124796	39.09608580916915	32.169671124269065
5	12.38102714243211	37.899148425211735	36.07369460572388	13.646129826632281
6	30.949610750946484	38.51216379073165	17.409546580072153	13.128678878249717
7	27.095216297189744	32.522494374892524	22.469331356976948	17.91295797094078
8	24.97946214058746	36.63101604278075	20.17205301092769	18.2174688057041
9	24.7790859829372	16.288131069595362	21.359502172690508	37.57328077477693
10-11	23.8130517264471	26.75165313585395	28.970850122221737	20.464445015477224
12-13	24.74603946536052	23.947998880112998	29.381025084112622	21.924936570413863
14-15	21.606560648605395	26.00466652555037	26.739133277887866	25.649639547956372
16-17	20.92457037898875	29.675867306952696	27.22005973376527	22.179502580293285
18-19	20.723461618384388	28.153937217454565	29.95237082651365	21.170230337647393
20-21	21.822441721797205	27.474795276711873	29.702483998500355	21.000279002990563
22-23	21.318054657095615	27.315760143124972	29.82795504651392	21.53823015326549
24-25	21.238140539241876	27.44909035689669	29.840575715757538	21.472193388103896
26-27	21.08973564082493	27.348938998209704	29.905244169915292	21.656081191050074
28-29	21.14273797493986	27.449312459247967	29.884746215934204	21.523203349877974
30-31	21.38294184045465	27.970648512619412	29.423952711223357	21.222456935702585
32-33	21.03661734627258	27.555296182787064	29.98628602824628	21.421800442694085
34-35	21.09206022807439	27.371155799151893	30.004002155155707	21.53278181761801
36-37	21.522625151288167	27.745062603503396	29.36250146564196	21.369810779566482
38-39	21.44109587182722	27.915360926837874	29.377329255742836	21.266213945592074
40-41	21.214883036727908	27.641697349568982	29.632052282793396	21.51136733090971
42-43	21.196661448666163	27.602810840456605	29.44924174264744	21.751285968229787
44-45	21.392837053951016	27.77173784022492	29.336897219480836	21.498527886343233
46-47	21.474674805610057	28.048542983794782	28.99178838747184	21.484993823123318
48-49	21.491834885940307	27.92708802627563	28.857756010599388	21.72332107718467
50-51	21.510562471146912	27.905109485515204	28.745815242375638	21.838512800962246
52-53	21.586847783016903	27.894619309798287	28.71813453130283	21.800398375881983
54-55	21.587615447605987	28.391276831812277	28.495422785342356	21.52568493523938
56-57	21.378518112525832	28.02227624275137	28.60374101179423	21.995464632928567
58-59	21.275237403489175	27.678864871161878	28.977938895227613	22.06795883012133
60-61	21.539415774835092	28.035731066158124	28.676207738711675	21.748645420295105
62-63	21.327184212304523	28.03796715716418	28.67447481034465	21.96037382018665
64-65	21.357348033113258	27.719806435674737	28.857214824430955	22.065630706781047
66-67	21.25278520499109	27.87503390684337	28.6056052855925	22.266575602573045
68-69	21.739192562544517	27.95805411511287	28.57356332946551	21.729189992877103
70-71	21.684654220935702	27.826918410081365	28.559087190352255	21.929340178630678
72-73	21.52911033593665	28.02192705529209	28.604906365109105	21.84405624366215
74-75	21.52701891033093	28.010176896845696	28.48387971789506	21.97892447492831
76-77	21.560256170859365	28.0236950536975	28.300883296847175	22.11516547859596
78-79	21.727373676581532	28.15013859338312	28.3840701770532	21.73841755298215
80-81	21.667628166470735	27.8591056453359	28.505393359984073	21.967872828209288
82-83	21.469754383789436	27.977515898668965	28.65901890111849	21.893710816423113
84-85	21.41773684752269	27.919713730405242	28.459581373101074	22.20296804897099
86-87	21.60634791005335	28.045200498621814	28.325729762379627	22.022721828945205
88-89	21.406770860075156	27.907928044353724	28.386786302115674	22.298514793455443
90-91	21.337815791972066	27.84745593589125	28.517790332924513	22.296937939212164
92-93	21.456120572004984	28.016933943814276	28.371815349292866	22.15513013488787
94-95	21.248475413345837	27.77857431409343	28.48790066718528	22.48504960537546
96-97	21.22658142866969	27.824009176098357	28.46554652132677	22.48386287390518
98-99	21.187613042227298	27.87620114178099	28.39766063742496	22.538525178566758
100	21.299068924395034	27.791943207391256	28.247648091977982	22.661339776235724
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	39.0
1	28.5
2	85.5
3	163.5
4	111.5
5	67.5
6	212.0
7	489.5
8	835.5
9	1006.5
10	826.5
11	646.5
12	648.0
13	749.0
14	897.5
15	1019.5
16	1185.0
17	1429.0
18	1691.5
19	1989.0
20	2502.5
21	3200.0
22	4215.0
23	5825.5
24	8024.5
25	10853.0
26	14536.0
27	19249.0
28	24955.0
29	30970.5
30	36581.5
31	42314.0
32	48179.0
33	53863.0
34	59075.5
35	63254.0
36	66642.5
37	69601.5
38	72032.5
39	72941.5
40	73263.0
41	73569.5
42	73283.5
43	72791.5
44	71255.5
45	70041.0
46	69171.0
47	67241.5
48	65550.0
49	64029.0
50	62429.0
51	59968.0
52	55692.0
53	52739.0
54	51460.0
55	49295.5
56	45473.0
57	41660.5
58	37721.5
59	33077.5
60	28780.0
61	24434.5
62	20873.5
63	17265.5
64	13740.5
65	11220.0
66	8840.0
67	6685.0
68	5111.5
69	4020.5
70	3034.0
71	2146.0
72	1696.0
73	1334.0
74	958.0
75	654.5
76	394.5
77	257.0
78	165.0
79	104.0
80	76.5
81	40.5
82	16.0
83	7.0
84	2.5
85	2.0
86	2.0
87	2.0
88	1.5
89	0.5
90	0.0
91	0.0
92	0.5
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.09348537704638521
2	0.0017437686910206569
3	0.0
4	2.906281151701095E-4
5	0.03816915912567438
6	2.906281151701095E-4
7	0.004310983708356623
8	6.781322687302555E-4
9	1.4531405758505474E-4
10-11	4.117231631576551E-4
12-13	0.0
14-15	1.4531405758505474E-4
16-17	0.0
18-19	1.4531405758505474E-4
20-21	0.0
22-23	2.179710863775821E-4
24-25	1.4531405758505474E-4
26-27	0.0014531405758505475
28-29	0.008718843455103285
30-31	0.03625585736747116
32-33	0.026180749374907362
34-35	0.029353439632181056
36-37	0.026737786595650074
38-39	0.025672150173359668
40-41	0.031048770304006695
42-43	0.02349243930958385
44-45	0.022039298733733302
46-47	0.01666267860308628
48-49	0.010850116299684088
50-51	0.007604769013617865
52-53	0.005425058149842044
54-55	0.004044574602784024
56-57	0.002542996007738458
58-59	0.0011382934510829287
60-61	7.75008307120292E-4
62-63	0.0012351694894729654
64-65	0.003100033228481168
66-67	6.781322687302555E-4
68-69	0.0011867314702779471
70-71	5.328182111452007E-4
72-73	6.054752399377281E-4
74-75	6.781322687302555E-4
76-77	6.054752399377281E-4
78-79	7.265702879252737E-5
80-81	5.328182111452007E-4
82-83	6.296942495352372E-4
84-85	3.148471247676186E-4
86-87	1.6953306718256388E-4
88-89	0.0
90-91	9.68760383900365E-5
92-93	7.265702879252737E-5
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2064494.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	70.62352493247192
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.03688517662016	57.231104807208986
2	12.44926644554207	17.584221584154534
3	3.2818491721949443	6.95327270511366
4	1.263051731411241	3.568046617772944
5	0.6050018918391346	2.13636830962469
6	0.3475488870580164	1.4727076494236815
7	0.22563771498473978	1.1154731552951536
8	0.15645515058870424	0.8839531382732003
9	0.10738900620643384	0.6825771141564116
>10	0.497743077629577	6.100946441754236
>50	0.022157895537825458	1.0663175541390382
>100	0.00673916169748605	0.7710444766811967
>500	6.867217241430952E-5	0.04432781984931465
>1k	2.0601651724292856E-4	0.3896386265529474
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3468	0.16798305056832327	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3019	0.14623437994976007	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	4.843801919501825E-5	0.0
4	0.0	0.0	0.0	4.843801919501825E-5	0.0
5	0.0	0.0	0.0	4.843801919501825E-5	0.0
6	0.0	0.0	0.0	4.843801919501825E-5	0.0
7	0.0	0.0	0.0	4.843801919501825E-5	0.0
8	0.0	0.0	0.0	4.843801919501825E-5	0.0
9	0.0	0.0	0.0	9.68760383900365E-5	0.0
10-11	0.0	0.0	0.0	9.68760383900365E-5	0.0
12-13	0.0	0.0	0.0	9.68760383900365E-5	0.0
14-15	0.0	0.0	0.0	1.4531405758505474E-4	0.0
16-17	0.0	0.0	0.0	2.179710863775821E-4	0.0
18-19	0.0	0.0	0.0	2.4219009597509122E-4	0.0
20-21	0.0	0.0	0.0	2.906281151701095E-4	4.843801919501825E-5
22-23	0.0	0.0	0.0	2.906281151701095E-4	4.843801919501825E-5
24-25	0.0	0.0	0.0	3.390661343651277E-4	4.843801919501825E-5
26-27	0.0	0.0	0.0	4.843801919501825E-4	4.843801919501825E-5
28-29	0.0	0.0	0.0	0.0010656364222904014	4.843801919501825E-5
30-31	0.0	0.0	0.0	0.004286764698759115	4.843801919501825E-5
32-33	0.0	0.0	0.0	0.010922773328476614	4.843801919501825E-5
34-35	0.0	0.0	0.0	0.019786930841164955	4.843801919501825E-5
36-37	0.0	0.0	0.0	0.03766055992412669	4.843801919501825E-5
38-39	0.0	0.0	0.0	0.06924214843927859	4.843801919501825E-5
40-41	0.0	0.0	0.0	0.1125457375996249	4.843801919501825E-5
42-43	0.0	0.0	0.0	0.15398446302096302	4.843801919501825E-5
44-45	0.0	0.0	0.0	0.1982810315748072	4.843801919501825E-5
46-47	0.0	0.0	0.0	0.25347615444753047	4.843801919501825E-5
48-49	0.0	0.0	0.0	0.31571900911312895	4.843801919501825E-5
50-51	0.0	0.0	0.0	0.3856877278403328	4.843801919501825E-5
52-53	0.0	0.0	0.0	0.4558986366635117	4.843801919501825E-5
54-55	0.0	0.0	0.0	0.5271025248801886	4.843801919501825E-5
56-57	0.0	0.0	0.0	0.6107549840299851	4.843801919501825E-5
58-59	0.0	0.0	0.0	0.7123052912723409	4.843801919501825E-5
60-61	0.0	0.0	0.0	0.8224775659314099	7.265702879252737E-5
62-63	0.0	0.0	0.0	0.933037344744039	9.68760383900365E-5
64-65	0.0	0.0	0.0	1.0468909088619294	9.68760383900365E-5
66-67	0.0	0.0	0.0	1.1761235440742381	9.68760383900365E-5
68-69	0.0	0.0	0.0	1.3251673291373094	9.68760383900365E-5
70-71	0.0	0.0	0.0	1.4856909247495997	9.68760383900365E-5
72-73	0.0	0.0	0.0	1.6587841863429973	9.68760383900365E-5
74-75	0.0	0.0	0.0	1.830593840427727	9.68760383900365E-5
76-77	0.0	0.0	0.0	2.013350486850531	9.68760383900365E-5
78-79	0.0	0.0	0.0	2.225339477857528	9.68760383900365E-5
80-81	0.0	0.0	0.0	2.4627584289419104	9.68760383900365E-5
82-83	0.0	0.0	0.0	2.7051422769937816	9.68760383900365E-5
84-85	0.0	0.0	0.0	2.9471386208920927	9.68760383900365E-5
86-87	0.0	0.0	0.0	3.1992585108021627	9.68760383900365E-5
88	0.0	0.0	0.0	3.4022380302388866	9.68760383900365E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2860	0.0	44.59066	1
GTATCAA	4845	0.0	40.891113	1
ATCAACG	5370	0.0	36.242157	3
TCAACGC	5370	0.0	36.242157	4
CAACGCA	5485	0.0	35.568	5
AACGCAG	5695	0.0	34.245663	6
TATCAAC	5965	0.0	32.942287	2
ACGCAGA	6550	0.0	29.416689	7
CGCAGAG	6635	0.0	29.03913	8
GTACATG	5010	0.0	26.863894	1
TACATGG	5070	0.0	24.849337	2
GTATAGG	665	0.0	24.767776	1
GCAGAGT	7835	0.0	24.59153	9
ACATGGG	5110	0.0	23.734865	3
TCCGTAC	110	0.0030566787	21.368069	3
GAGTACT	5285	0.0	21.207235	12-13
AGAGTAC	7005	0.0	20.494804	10-11
CAGAGTA	7805	0.0	19.86926	10-11
CATGGGG	3430	0.0	19.050598	4
GTACTTT	6090	0.0	18.40398	14-15
>>END_MODULE