FastQCFastQC Report
Wed 25 May 2016
SRR1033120_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033120_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences422017
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA75561.7904491999137475No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8380.19857019977868193No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7510.17795491650810275No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5090.12061125499683663No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACAAA4550.037.290551
GAGAACA7100.032.5271071
CCCTAGA1452.5807822E-829.2537941
ACAACAC6700.027.3531685
TAACAAC2600.027.1910251
GTATAAG1257.836728E-626.3934211
TTGTACG459.572068E-426.0846440-41
GTACAAG2750.025.707881
GTACATG26500.025.2551611
TACATGG26200.024.0451492
GTACCCA1802.0969674E-723.5655561
ATAATAC1602.036968E-623.495673
GTATCAA25050.022.7659261
CGTATCA853.2952903E-622.23490192-93
GTACATA1503.2150987E-521.9945181
AACAACA10350.021.7930854
CATGGGG10400.021.6883094
GGTATCA19450.021.5664221
GGAAACA7000.021.545651
ACATGGG27350.021.305033
Produced by FastQC (version 0.11.5)