##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1033120_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 422017 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.22755007499698 33.0 31.0 34.0 22.0 34.0 2 30.30630282666338 33.0 31.0 34.0 24.0 34.0 3 30.408642305878672 34.0 31.0 34.0 23.0 34.0 4 33.4384918143108 37.0 35.0 37.0 24.0 37.0 5 33.86895314643723 37.0 35.0 37.0 29.0 37.0 6 33.71432430447115 37.0 35.0 37.0 27.0 37.0 7 33.83329107595192 37.0 35.0 37.0 29.0 37.0 8 33.61956034946459 37.0 35.0 37.0 27.0 37.0 9 34.994483634545524 39.0 37.0 39.0 22.0 39.0 10-11 35.15879336614402 39.0 37.0 39.0 27.0 39.0 12-13 35.16998485842987 39.0 37.0 39.0 26.5 39.0 14-15 36.56873301312507 41.0 38.0 41.0 27.0 41.0 16-17 36.69321615006031 41.0 38.0 41.0 28.5 41.0 18-19 36.69459879578311 41.0 38.0 41.0 29.5 41.0 20-21 36.72498856681129 40.5 38.0 41.0 30.0 41.0 22-23 36.708356535400235 40.0 38.0 41.0 29.0 41.0 24-25 36.7138041832438 40.0 38.0 41.0 29.0 41.0 26-27 36.53550567868119 40.0 37.0 41.0 29.0 41.0 28-29 36.35943338775452 40.0 37.0 41.0 28.5 41.0 30-31 36.200779826405096 40.0 37.0 41.0 28.0 41.0 32-33 36.09862161950822 40.0 37.0 41.0 27.0 41.0 34-35 35.934858074437756 40.0 37.0 41.0 26.5 41.0 36-37 35.74258145998858 40.0 37.0 41.0 25.0 41.0 38-39 35.565308980443916 40.0 37.0 41.0 24.5 41.0 40-41 35.43825841139101 40.0 36.0 41.0 23.5 41.0 42-43 35.26799394337195 40.0 36.0 41.0 22.0 41.0 44-45 35.14769191762417 40.0 35.5 41.0 20.5 41.0 46-47 34.99576201906558 40.0 35.0 41.0 18.0 41.0 48-49 34.83923988844052 40.0 35.0 41.0 12.0 41.0 50-51 34.70957449581415 40.0 35.0 41.0 8.0 41.0 52-53 34.56228303599144 40.0 35.0 41.0 2.0 41.0 54-55 34.4115853152835 40.0 35.0 41.0 2.0 41.0 56-57 34.25992910238213 39.5 35.0 41.0 2.0 41.0 58-59 34.067748455630934 39.0 35.0 41.0 2.0 41.0 60-61 33.85119675273745 39.0 35.0 41.0 2.0 41.0 62-63 33.583112765599495 38.5 34.0 41.0 2.0 41.0 64-65 33.30831459396185 38.0 34.0 41.0 2.0 41.0 66-67 33.01135499280835 37.0 34.0 40.0 2.0 41.0 68-69 32.68635505204767 37.0 34.0 40.0 2.0 41.0 70-71 32.33224372477886 36.0 33.0 39.5 2.0 41.0 72-73 31.962040628695053 36.0 33.0 39.0 2.0 41.0 74-75 31.595112282206642 35.0 33.0 39.0 2.0 40.5 76-77 31.24999703803401 35.0 33.0 37.5 2.0 40.0 78-79 30.85915022380615 35.0 32.0 37.0 2.0 39.0 80-81 30.495238343479052 35.0 32.0 36.5 2.0 39.0 82-83 30.248258956392753 35.0 32.0 36.0 2.0 38.5 84-85 29.950159590727388 35.0 32.0 36.0 2.0 37.0 86-87 29.682170386501017 35.0 31.0 35.5 2.0 37.0 88-89 29.292468075930593 35.0 31.0 35.0 2.0 36.5 90-91 29.062710743879983 35.0 31.0 35.0 2.0 36.0 92-93 28.82848321276157 35.0 31.0 35.0 2.0 36.0 94-95 28.59532435897132 35.0 30.0 35.0 2.0 36.0 96-97 28.377390010355033 35.0 30.0 35.0 2.0 35.0 98-99 28.142284552518028 35.0 29.5 35.0 2.0 35.0 100 27.94704241772251 35.0 29.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.42320575000688976 1101 2 0.39109603586053154 1101 3 0.445349235518659 1101 4 0.5695140579601485 1101 5 0.4105948359450835 1101 6 0.29904176963606943 1101 7 0.6251972307074851 1101 8 0.5227318883612284 1101 9 0.30715954391205713 1101 10-11 0.3294344131584026 1101 12-13 0.5455177399240299 1101 14-15 0.3222186407729595 1101 16-17 0.30054168772760903 1101 18-19 0.37584603472142675 1101 20-21 0.23507668922797365 1101 22-23 0.16606854235133994 1101 24-25 0.2246968059019281 1101 26-27 0.12880072977041834 1101 28-29 0.37388253184737863 1101 30-31 0.36733968997175026 1101 32-33 0.49643489197615054 1101 34-35 0.3912955863891838 1101 36-37 0.44719947331005727 1101 38-39 0.5227354690054682 1101 40-41 0.5287699245499269 1101 42-43 0.5645885891630726 1101 44-45 0.5773715546270353 1101 46-47 0.6806482898025976 1101 48-49 0.654572105802778 1101 50-51 0.7319539601923921 1101 52-53 0.6813862518992764 1101 54-55 0.64483014411487 1101 56-57 0.5733691016189155 1101 58-59 0.49689746339169005 1101 60-61 0.6071081758012298 1101 62-63 0.4154184977205517 1101 64-65 0.551148169131011 1101 66-67 0.5477458629119738 1101 68-69 0.5202527683654736 1101 70-71 0.5113549887560893 1101 72-73 0.6201042080999173 1101 74-75 0.5251883824256787 1101 76-77 0.4130573785297109 1101 78-79 0.4061379399889695 1101 80-81 0.3458494255167892 1101 82-83 0.445088832365407 1101 84-85 0.42922584473434 1101 86-87 0.5262424524833591 1101 88-89 0.557233516260009 1101 90-91 0.39790044532360724 1101 92-93 0.632481299007452 1101 94-95 0.483202747926331 1101 96-97 0.43883014910220197 1101 98-99 0.5845881792398622 1101 100 0.5925138012358886 1102 1 0.40144885521628737 1102 2 0.2657232064427575 1102 3 0.1982535596589372 1102 4 0.2510053756312445 1102 5 0.17047907155965447 1102 6 0.18153410875762432 1102 7 0.5157794574694847 1102 8 0.13332092479296165 1102 9 0.022317868733303214 1102 10-11 0.2867173549357247 1102 12-13 0.4121537630769083 1102 14-15 0.2670705304563086 1102 16-17 0.2029523108123925 1102 18-19 0.25618651820797567 1102 20-21 0.44224046178500487 1102 22-23 0.12245601255902727 1102 24-25 0.32606895435272776 1102 26-27 0.26176293610340906 1102 28-29 0.0780738835668231 1102 30-31 0.0036794924913294835 1102 32-33 0.18958436422674652 1102 34-35 0.12782605966123128 1102 36-37 0.12177897620416189 1102 38-39 -0.029161024014612735 1102 40-41 0.006996005584994691 1102 42-43 -0.08489047110251136 1102 44-45 0.006261578460879491 1102 46-47 -0.018054468113639643 1102 48-49 4.0799276225556014E-4 1102 50-51 -0.031648355095320824 1102 52-53 -0.009386645615194311 1102 54-55 -0.060685688367250634 1102 56-57 -0.029300288915642625 1102 58-59 -0.03541782431685192 1102 60-61 -0.12000111939795488 1102 62-63 -0.22358048082898563 1102 64-65 -0.07621989351795122 1102 66-67 0.02036758575840736 1102 68-69 0.05694326887620349 1102 70-71 0.09275097105094687 1102 72-73 0.09448963540669553 1102 74-75 -0.1139331701790205 1102 76-77 -0.2627989374389692 1102 78-79 -0.3570694144543438 1102 80-81 -0.10873688704705131 1102 82-83 -0.12538443968090007 1102 84-85 -0.01915004903480977 1102 86-87 -0.04246711510261392 1102 88-89 -0.007134886872439239 1102 90-91 0.10792768400253294 1102 92-93 0.16658072018552517 1102 94-95 0.1954976066256826 1102 96-97 -6.48911163377619E-4 1102 98-99 0.05918811114316469 1102 100 0.09622507123929225 1103 1 0.17662368039111342 1103 2 0.34495397567352626 1103 3 0.33867314007851945 1103 4 0.115970410596276 1103 5 0.06264690372748305 1103 6 0.12335229057580932 1103 7 -0.11156319987316721 1103 8 0.10919505066708268 1103 9 0.05112905754449315 1103 10-11 -0.041499428281056794 1103 12-13 0.10907683999998596 1103 14-15 0.08406353744931039 1103 16-17 0.289001261861344 1103 18-19 0.09908861611007325 1103 20-21 0.1718908114353539 1103 22-23 0.1321413272443408 1103 24-25 0.15232769561146853 1103 26-27 0.12004964939012552 1103 28-29 0.28915779965073796 1103 30-31 0.2867564155682487 1103 32-33 0.3702137348561152 1103 34-35 0.3566022834374536 1103 36-37 0.5379677873929793 1103 38-39 0.6736711438175575 1103 40-41 0.49860439719338245 1103 42-43 0.5342004379884031 1103 44-45 0.4286392008385036 1103 46-47 0.30806441300524057 1103 48-49 0.4220513494056135 1103 50-51 0.2903796169326469 1103 52-53 0.3582706970421512 1103 54-55 0.3590695563879933 1103 56-57 0.3828325782172257 1103 58-59 0.5370646931656609 1103 60-61 0.40992895053211953 1103 62-63 0.3882027359542306 1103 64-65 0.40713674983869197 1103 66-67 0.3585843689752011 1103 68-69 0.4956495625824928 1103 70-71 0.4950936283936045 1103 72-73 0.6012728521899078 1103 74-75 0.7568710256251769 1103 76-77 0.7755577059176737 1103 78-79 0.6337347813498546 1103 80-81 0.7552840919739268 1103 82-83 0.700454721158259 1103 84-85 0.6045912097064488 1103 86-87 0.5026377800022814 1103 88-89 0.6271308473932926 1103 90-91 0.6628227888976355 1103 92-93 0.7454268740316792 1103 94-95 0.604483620611699 1103 96-97 0.756344095829629 1103 98-99 0.631041257996312 1103 100 0.8105607355749562 1104 1 0.3841745897186719 1104 2 0.36471720736569324 1104 3 0.23802590237544408 1104 4 0.2587857171171706 1104 5 0.3123895468711453 1104 6 -0.16837205952418088 1104 7 0.032743001539628835 1104 8 -0.024331744027222157 1104 9 0.03444523375407016 1104 10-11 0.186860409850361 1104 12-13 0.06376428453514649 1104 14-15 -0.03452768520281779 1104 16-17 0.26940516421955607 1104 18-19 -0.2010708268158652 1104 20-21 -0.08761955975014502 1104 22-23 -0.18606267687336242 1104 24-25 -0.23738897519656632 1104 26-27 0.0585665047851478 1104 28-29 -0.06510870372743938 1104 30-31 -0.03179812633654677 1104 32-33 0.04077033561576826 1104 34-35 0.07696926839834362 1104 36-37 -0.061465140085637415 1104 38-39 0.003281677108290637 1104 40-41 -0.03744331144315538 1104 42-43 -0.0776669670262109 1104 44-45 -0.10429064427408719 1104 46-47 -0.1669438697441592 1104 48-49 -0.15973044747770615 1104 50-51 -0.061397624438491505 1104 52-53 -0.015708749906842456 1104 54-55 -0.17019960930249312 1104 56-57 -0.05096311002008491 1104 58-59 -0.15629461250328802 1104 60-61 -0.10724000572313486 1104 62-63 -0.18712147452226446 1104 64-65 -0.21206976276884149 1104 66-67 -0.14946320950384973 1104 68-69 -0.3970222748863037 1104 70-71 -0.22925795065340537 1104 72-73 -0.2837152561484544 1104 74-75 -0.2937055128955244 1104 76-77 -0.2527204879696576 1104 78-79 -0.25878914987966084 1104 80-81 -0.24121496734239045 1104 82-83 -0.3026202495563055 1104 84-85 -0.3897868741386432 1104 86-87 -0.5972817590035504 1104 88-89 -0.39966680503887275 1104 90-91 -0.3795050186585982 1104 92-93 -0.35583121643961135 1104 94-95 -0.3267651221852681 1104 96-97 -0.4978416507419041 1104 98-99 -0.39704169965517266 1104 100 -0.2990525783530771 1105 1 -0.19253576712654308 1105 2 0.05518197321495677 1105 3 0.08389806440791858 1105 4 -0.015519575371321537 1105 5 0.17040067566036043 1105 6 0.0889033232910208 1105 7 0.2184388875916028 1105 8 -0.07543830933941109 1105 9 -0.3651810005566105 1105 10-11 -0.3440846954185588 1105 12-13 -0.28798795258719423 1105 14-15 -0.04255859663550865 1105 16-17 -0.22666777059873056 1105 18-19 -0.356023643106667 1105 20-21 -0.23964707025178456 1105 22-23 -0.2310702952956376 1105 24-25 -0.30416171050480045 1105 26-27 -0.23195634627258954 1105 28-29 -0.0723693547868649 1105 30-31 -0.08626774103800017 1105 32-33 -0.24423442063682188 1105 34-35 -0.28336947780296384 1105 36-37 -0.14543193891719852 1105 38-39 -0.3622636924904157 1105 40-41 -0.3679776111796613 1105 42-43 -0.3275890991742756 1105 44-45 -0.38377309664842585 1105 46-47 -0.3351342208650294 1105 48-49 -0.35470733966650414 1105 50-51 -0.356116921354932 1105 52-53 -0.44535830248236863 1105 54-55 -0.5259125841911612 1105 56-57 -0.3256051283548018 1105 58-59 -0.4359243824353456 1105 60-61 -0.5754993856424875 1105 62-63 -0.4692219703510716 1105 64-65 -0.5153545233988481 1105 66-67 -0.4003786828982996 1105 68-69 -0.5535029847043873 1105 70-71 -0.41961748967572277 1105 72-73 -0.308334124581247 1105 74-75 -0.35350802318670915 1105 76-77 -0.2227476785817366 1105 78-79 -0.09061984412417345 1105 80-81 -0.22209735742244874 1105 82-83 -0.26505427233581 1105 84-85 -0.3178580243099489 1105 86-87 -0.13602206761279234 1105 88-89 -0.18232710758704584 1105 90-91 -0.18574033951623647 1105 92-93 -0.21810192718368882 1105 94-95 -0.24813121894343837 1105 96-97 -0.1316228294507269 1105 98-99 -0.2124612179688512 1105 100 -0.020479285763570942 1106 1 0.09878810133601945 1106 2 0.008132666900998231 1106 3 0.15204513393831576 1106 4 0.08348135493648812 1106 5 -0.03596586561699411 1106 6 -0.08662553647518934 1106 7 -0.1299411633730827 1106 8 0.14862025960449188 1106 9 -0.19474052003240416 1106 10-11 -0.2445538941404024 1106 12-13 -0.36422465525273395 1106 14-15 -0.07629236680902807 1106 16-17 -0.29281265592818073 1106 18-19 -0.25452301616009265 1106 20-21 -0.13858732831108256 1106 22-23 -0.09545973023476506 1106 24-25 0.03980140076809846 1106 26-27 0.17828917409305944 1106 28-29 0.16374276752838313 1106 30-31 0.28903056418386086 1106 32-33 0.045297594086314064 1106 34-35 0.1061261335710526 1106 36-37 0.17550403163653527 1106 38-39 0.0075943344480577935 1106 40-41 0.060728452000368804 1106 42-43 -0.03198581478321216 1106 44-45 -0.03364972974311087 1106 46-47 -0.08944397291777761 1106 48-49 -0.17540871085932253 1106 50-51 -0.22632684733478925 1106 52-53 -0.2688101489411352 1106 54-55 -0.24172781697035362 1106 56-57 -0.13830989719354392 1106 58-59 -0.19569868167087634 1106 60-61 -0.14418441577637253 1106 62-63 -0.2281185443914424 1106 64-65 -0.18566556979289572 1106 66-67 -0.06670558497329182 1106 68-69 -0.10225111397672748 1106 70-71 -0.09360527432968979 1106 72-73 -0.043374370506093385 1106 74-75 -0.04187108592181232 1106 76-77 -0.10451364549513897 1106 78-79 0.03196236677256081 1106 80-81 -0.002099784312385111 1106 82-83 -0.05903558528326158 1106 84-85 -0.09670363366156565 1106 86-87 -0.048047307268170414 1106 88-89 -0.25558278044228544 1106 90-91 -0.18896810313715662 1106 92-93 -0.10851166710198612 1106 94-95 -0.14004563062487208 1106 96-97 -0.0032727101286411653 1106 98-99 -0.03589688516134615 1106 100 0.12689765069236003 1107 1 0.4081079814710584 1107 2 0.3546359733026492 1107 3 0.36026119432598236 1107 4 0.287671571022166 1107 5 0.19986971202338566 1107 6 0.3779118833935655 1107 7 0.33013545916607967 1107 8 0.16559860046098862 1107 9 0.6577370685070889 1107 10-11 0.5465915243122268 1107 12-13 0.39508321309811123 1107 14-15 0.3664809645012639 1107 16-17 0.3688267629543418 1107 18-19 0.3931877906524903 1107 20-21 0.16990057771884182 1107 22-23 0.14632989917008388 1107 24-25 0.05709963440362742 1107 26-27 0.1700705849087285 1107 28-29 0.1684754105002284 1107 30-31 0.3209819101358846 1107 32-33 0.23820305502272987 1107 34-35 0.3666554107145288 1107 36-37 0.17688854781450658 1107 38-39 0.2746605509889193 1107 40-41 0.12141704333418346 1107 42-43 0.18198072923681252 1107 44-45 0.3035046153617671 1107 46-47 0.3190881870538931 1107 48-49 0.12569005129470412 1107 50-51 0.09266947326604935 1107 52-53 0.17168220777092102 1107 54-55 0.07711298263514976 1107 56-57 0.19428376311319795 1107 58-59 0.33255050595319346 1107 60-61 0.2363653446201397 1107 62-63 0.2924608029261364 1107 64-65 0.21719449924325573 1107 66-67 0.3383924691176645 1107 68-69 0.10186197381045048 1107 70-71 0.2462586764093544 1107 72-73 0.25802278965524295 1107 74-75 0.3842147161492697 1107 76-77 0.4776874191282623 1107 78-79 0.3976541823221318 1107 80-81 0.20039360920630855 1107 82-83 0.26372484916400296 1107 84-85 0.40510698475566187 1107 86-87 0.3857740838228807 1107 88-89 0.34228544396313154 1107 90-91 0.24487098953059672 1107 92-93 0.22227137478753178 1107 94-95 0.415801579480199 1107 96-97 0.3719299097047113 1107 98-99 0.2773628601888305 1107 100 0.3965078366853554 1108 1 -0.15379056185519246 1108 2 -0.22479726545175893 1108 3 -0.025091339716496464 1108 4 -0.4829432711418349 1108 5 -0.06924334342993888 1108 6 -0.4059329280223878 1108 7 -0.3318402510662537 1108 8 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-0.6498686461739318 2214 72-73 -0.7166467282296693 2214 74-75 -0.721588672571361 2214 76-77 -0.7672008513145663 2214 78-79 -0.6633253953155851 2214 80-81 -0.7259498057073372 2214 82-83 -0.696508841594774 2214 84-85 -0.9442218193697371 2214 86-87 -0.9007805122317976 2214 88-89 -0.9353645519442075 2214 90-91 -1.11497901456206 2214 92-93 -1.2969958348383983 2214 94-95 -1.103700957967618 2214 96-97 -1.0271441264743828 2214 98-99 -1.1326181089525313 2214 100 -1.367411292397069 2215 1 -0.28438071990832725 2215 2 -0.4109252625816886 2215 3 -0.5593348571461902 2215 4 -0.19759657072527403 2215 5 0.060172876449279045 2215 6 -0.21025796339998237 2215 7 -0.3129316747403408 2215 8 -0.1157177649768002 2215 9 -0.3235354424463779 2215 10-11 -0.26608587316776067 2215 12-13 -0.12266465610182564 2215 14-15 -0.31949960958641554 2215 16-17 -0.17958269986883124 2215 18-19 -0.22700598119862292 2215 20-21 -0.008224764894315229 2215 22-23 -0.03440133375001153 2215 24-25 0.09309482665275226 2215 26-27 -0.15171676956951075 2215 28-29 -0.270143538697603 2215 30-31 -0.24877006839165006 2215 32-33 -0.2680989967986491 2215 34-35 -0.24371678391816687 2215 36-37 -0.2658925167937056 2215 38-39 -0.208171230518289 2215 40-41 -0.17670919261106377 2215 42-43 -0.2769199038107928 2215 44-45 -0.16329693091486774 2215 46-47 -0.1899842093906443 2215 48-49 -0.23571353583968602 2215 50-51 -0.11690826489830641 2215 52-53 -0.25387988083949864 2215 54-55 -0.09993325542160392 2215 56-57 -0.07648519278460952 2215 58-59 -0.027207522868948786 2215 60-61 -0.2064644471928716 2215 62-63 -0.14857217320971472 2215 64-65 -0.25271645178996494 2215 66-67 -0.19323554740086024 2215 68-69 -0.07360266039035679 2215 70-71 -0.14186569165971008 2215 72-73 -0.17039999193724498 2215 74-75 -0.1856397925383817 2215 76-77 -0.24843625051753193 2215 78-79 -0.1886122580337357 2215 80-81 -0.30079005581040263 2215 82-83 -0.2638202336519413 2215 84-85 -0.30288610410245553 2215 86-87 -0.3575157740155035 2215 88-89 -0.41742446674426503 2215 90-91 -0.534583590623626 2215 92-93 -0.5924627167952465 2215 94-95 -0.7618344607847156 2215 96-97 -1.050850667631451 2215 98-99 -0.9899099187642655 2215 100 -0.8734165143291861 2216 1 -0.3224133553460504 2216 2 -0.120210408248699 2216 3 -0.25292472253565634 2216 4 -0.18576204766064564 2216 5 -0.25469259491690366 2216 6 0.03637841125562602 2216 7 -0.006490418200641557 2216 8 -0.10236973335692312 2216 9 -0.22117705541406707 2216 10-11 -0.17887977064885519 2216 12-13 -0.11419048238904139 2216 14-15 -0.07651678561532549 2216 16-17 -0.13306114875188513 2216 18-19 -0.16969576763666794 2216 20-21 -0.28372509085557596 2216 22-23 -0.42505853061423693 2216 24-25 -0.6094483263590291 2216 26-27 -0.6477454476082372 2216 28-29 -0.4780011706136307 2216 30-31 -0.6030137891329446 2216 32-33 -0.4515676832239208 2216 34-35 -0.37080973365461745 2216 36-37 -0.39973237317418864 2216 38-39 -0.430670092110887 2216 40-41 -0.48488263020832534 2216 42-43 -0.451341936826239 2216 44-45 -0.5711195097106696 2216 46-47 -0.36678379399478445 2216 48-49 -0.3994460051845863 2216 50-51 -0.4107746240584831 2216 52-53 -0.3688254502233832 2216 54-55 -0.3131266601934115 2216 56-57 -0.3819420135648883 2216 58-59 -0.3085374775619769 2216 60-61 -0.1062198943494792 2216 62-63 -0.3529474250516387 2216 64-65 -0.32022810613344177 2216 66-67 -0.2591681733381961 2216 68-69 -0.4694523054365689 2216 70-71 -0.47602512111284767 2216 72-73 -0.36810532295991294 2216 74-75 -0.33851398459671955 2216 76-77 -0.2769611365948954 2216 78-79 -0.20780056536115055 2216 80-81 -0.21374201993173259 2216 82-83 -0.2604443233355127 2216 84-85 -0.35759763657900834 2216 86-87 -0.4647267250033771 2216 88-89 -0.44844320214562217 2216 90-91 -0.35210425394659595 2216 92-93 -0.21797500012929305 2216 94-95 -0.20508526094589286 2216 96-97 -0.22578293648560788 2216 98-99 -0.13028947526039403 2216 100 -0.08660828110472352 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 11529.0 3 1548.0 4 1191.0 5 1222.0 6 2685.0 7 5549.0 8 5949.0 9 3441.0 10 2147.0 11 2030.0 12 2107.0 13 1672.0 14 1595.0 15 1457.0 16 1431.0 17 1369.0 18 1191.0 19 1035.0 20 856.0 21 938.0 22 1182.0 23 2095.0 24 3919.0 25 3522.0 26 3480.0 27 2712.0 28 2935.0 29 3477.0 30 4204.0 31 5085.0 32 6816.0 33 9377.0 34 12790.0 35 22843.0 36 35510.0 37 72945.0 38 123489.0 39 54567.0 40 127.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.40863379819602 22.318163879550955 13.215778387451085 21.05742393480194 2 15.520206872827776 25.18771573965339 37.37921544903503 21.9128619384838 3 17.648913821801333 31.178127534060142 28.605537825367396 22.567420818771133 4 12.134990209511708 21.79699315851109 35.56554885998891 30.5024677719883 5 12.01650909849512 42.578965834415925 32.19914277857326 13.205382288515699 6 27.574848454688073 41.01368291839066 16.951273264989833 14.460195361931438 7 23.9572126617914 39.066195253960956 20.58797044507742 16.388621639170225 8 22.85494229447591 39.471526411830226 19.757091736379365 17.9164395573145 9 23.45506683097924 22.216797800739407 19.541899706133282 34.786235662148066 10-11 21.577600845608842 32.47393910810545 26.021668726574234 19.92679131971148 12-13 22.32308749644516 31.334368186557963 26.018342970897717 20.324201346099155 14-15 20.829650028794948 32.47714771216427 23.990932553460254 22.702269705580534 16-17 19.548759028078138 35.16669727868988 24.748458042765982 20.536085650465992 18-19 19.609890083847205 33.605832808004585 27.082908725512965 19.70136838263524 20-21 21.68042490561208 32.38222544551335 26.33345894688443 19.603890701990146 22-23 21.043900508453888 32.51948322789423 26.04904903362953 20.38756723002236 24-25 21.07921384598065 32.528455718103224 25.962872768425854 20.429457667490265 26-27 20.533496701046563 32.40321173972395 26.439618724404674 20.623672834824813 28-29 20.440309702739405 32.50231660872183 26.747765746421997 20.309607942116774 30-31 20.62128950455628 33.42497244901587 26.07839884345116 19.87533920297669 32-33 20.09833489561546 33.15336475278218 26.675088374606414 20.07321197699594 34-35 20.141292511283613 32.863780226395065 26.723409600164942 20.271517662156384 36-37 20.594201593448 32.92754598580969 26.235253970586143 20.24299845015617 38-39 20.21191255703948 32.725583666001924 26.526631853476516 20.535871923482077 40-41 19.913171651871405 32.69310065358238 26.937760378404768 20.455967316141447 42-43 19.993767573133265 32.590142101702305 26.803464091860757 20.612626233303672 44-45 19.73543738499145 32.8299123535622 26.81895684506001 20.615693416386343 46-47 19.866037574884356 32.90195836808979 26.87222738302874 20.359776673997118 48-49 19.868057341028408 32.319908815489995 26.72419410831478 21.08783973516682 50-51 19.920093082553894 32.09760397733601 26.29558450298703 21.68671843712307 52-53 19.887698627572487 31.721879335860255 26.30811904087661 22.08230299569065 54-55 19.95805736898852 31.83540479378206 26.506557979170864 21.699979858058555 56-57 20.234616961330026 31.566301032921256 26.088429812775615 22.110652192973102 58-59 20.01978649542067 31.465503963223185 26.452056255257638 22.062653286098506 60-61 20.445233801655437 31.71126745544463 26.027895536287655 21.815603206612273 62-63 20.357508459635454 31.57055383361295 26.059705595207628 22.012232111543966 64-65 20.52146054449334 31.508126642449056 26.08096548161523 21.88944733144237 66-67 20.579774365001693 31.653708807158143 25.88143854751913 21.885078280321036 68-69 21.218364928909953 32.0186018957346 25.651895734597158 21.11113744075829 70-71 21.14219837820674 32.04416092814726 25.621205586703383 21.192435106942618 72-73 21.139890356879235 31.97197666855053 25.89018389570752 20.997949078862714 74-75 21.32479786921451 32.05360612695614 25.730087866465723 20.891508137363626 76-77 21.84955195444544 31.501340641398862 25.630006670624017 21.019100733531676 78-79 22.087822378936465 31.28392332218403 26.037234641273272 20.59101965760623 80-81 22.159531183723182 30.769741160524077 26.221389150211255 20.849338505541482 82-83 21.971685136058973 30.871234785411055 26.112991686107613 21.044088392422356 84-85 22.05722748815166 30.671090047393363 26.131753554502367 21.13992890995261 86-87 22.28129250572272 30.519049672750363 26.15295662105867 21.04670120046824 88-89 22.26093694123257 30.1184884968411 26.17308380021457 21.447490761711766 90-91 22.33255087284679 30.156400697657503 26.151307750173757 21.35974067932194 92-93 22.591674216775548 30.136378808831243 26.0880501645129 21.183896809880313 94-95 21.875264469695797 30.386331371738006 26.096647805631612 21.641756352934586 96-97 21.701190855053238 30.64136442357776 26.052561631606107 21.60488308976289 98-99 21.457773137288555 31.169188740774644 25.634213437076873 21.738824684859928 100 21.42545537763983 31.35138872003185 25.58649016536865 21.636665736959667 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 5906.0 1 3799.5 2 1742.5 3 1565.0 4 1105.5 5 718.0 6 555.5 7 665.5 8 873.0 9 909.0 10 615.5 11 316.0 12 243.5 13 224.0 14 241.5 15 263.0 16 290.0 17 339.5 18 399.0 19 490.0 20 623.5 21 809.5 22 1116.5 23 1489.5 24 1874.5 25 2428.0 26 3211.5 27 4195.0 28 5520.0 29 6789.5 30 7665.5 31 8385.5 32 9255.0 33 10307.0 34 11382.0 35 12552.5 36 13474.5 37 14267.0 38 14560.0 39 14222.0 40 13948.0 41 13871.0 42 13948.0 43 13702.0 44 13306.5 45 13229.0 46 13198.5 47 13011.5 48 13322.5 49 13859.5 50 14212.5 51 13131.0 52 10880.0 53 9508.0 54 8723.5 55 8011.0 56 7547.5 57 6965.5 58 6489.0 59 6363.5 60 5666.0 61 4566.5 62 3730.5 63 3139.0 64 2837.0 65 2772.5 66 2440.5 67 1659.0 68 969.5 69 783.5 70 688.5 71 491.0 72 377.5 73 346.0 74 262.0 75 278.0 76 312.5 77 206.5 78 127.5 79 193.0 80 179.0 81 107.5 82 116.0 83 63.0 84 13.0 85 8.5 86 5.0 87 2.5 88 2.0 89 2.0 90 3.0 91 2.5 92 0.5 93 0.0 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3296075750502942 2 0.1189525540440314 3 0.062319764369681785 4 0.04241535293601917 5 0.04478492572574091 6 0.007345675648137397 7 0.005450017416360004 8 0.011136992111692183 9 0.013506564901413925 10-11 0.017179402725482622 12-13 0.013506564901413925 14-15 0.01611309497010784 16-17 0.0164685308885661 18-19 0.013980479459358274 20-21 0.020852240549551322 22-23 0.012203299867066966 24-25 0.021207676468009584 26-27 0.015402223133191317 28-29 0.015165265854219144 30-31 0.017060924085996534 32-33 0.02168159102595393 34-35 0.013151128982955663 36-37 0.00924133387991479 38-39 0.011966342588094793 40-41 0.008293504764026094 42-43 0.00722719700865131 44-45 0.00888589796145653 46-47 0.007819590206081747 48-49 0.0036728378240686987 50-51 0.006160889253276527 52-53 0.006516325171734788 54-55 0.0028434873476660895 56-57 0.002369572789721741 58-59 0.0028434873476660895 60-61 0.0033174019056104375 62-63 0.0030804446266382633 64-65 0.002369572789721741 66-67 0.001895658231777393 68-69 0.00402827374252696 70-71 0.0035543591845826113 72-73 0.002725008708180002 74-75 0.0030804446266382633 76-77 0.004146752382013047 78-79 0.004146752382013047 80-81 0.004265231021499134 82-83 0.0029619659871521764 84-85 0.00402827374252696 86-87 0.0035543591845826113 88-89 0.6082693351215709 90-91 0.6192878485937771 92-93 0.6153780534907362 94-95 0.6031747536236692 96-97 0.5991464798811422 98-99 0.5973693002888509 100 0.5995019157996005 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 422017.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.59514499985828 #Duplication Level Percentage of deduplicated Percentage of total 1 89.04342804594505 61.07946858096048 2 6.577017525197591 9.023029416150754 3 1.3545413392950778 2.7874487873174427 4 0.6387201231867503 1.752523978572899 5 0.4115738840897534 1.4115985128645747 6 0.2943478158865353 1.2114498666677085 7 0.23275399327517815 1.1176055742604818 8 0.1956572983408703 1.0736912599978023 9 0.14106907963047424 0.8708988575024041 >10 1.052404575729162 13.55476535998094 >50 0.04187482042540471 1.942793252314002 >100 0.015227207414914736 1.882971171480285 >500 0.0010382186873805503 0.49804255140766995 >1k 0.0 0.0 >5k 3.4607289579351673E-4 1.7937128305225714 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 7556 1.7904491999137475 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 838 0.19857019977868193 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 751 0.17795491650810275 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 509 0.12061125499683663 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 3.5543591845826113E-4 0.0 14-15 0.0 0.0 0.0 7.108718369165223E-4 0.0 16-17 0.0 0.0 0.0 0.0014217436738330445 0.0 18-19 0.0 0.0 0.0 0.0016587009528052188 0.0 20-21 0.0 0.0 0.0 0.003435880545096525 0.0 22-23 0.0 0.0 0.0 0.007464154287623485 0.0 24-25 0.0 0.0 0.0 0.016231573609593927 0.0 26-27 0.0 0.0 0.0 0.034714241369423506 0.0 28-29 0.0 0.0 0.0 0.06563716627529223 0.0 30-31 0.0 0.0 0.0 0.13423629853773664 0.0 32-33 0.0 0.0 0.0 0.23743119353011843 0.0 34-35 0.0 0.0 0.0 0.40567086160036203 0.0 36-37 0.0 0.0 0.0 0.6284107038342057 0.0 38-39 0.0 0.0 0.0 0.9304127558842417 0.0 40-41 0.0 0.0 0.0 1.2428409282090533 0.0 42-43 0.0 0.0 0.0 1.5049156787522777 0.0 44-45 0.0 0.0 0.0 1.7713741389564874 0.0 46-47 0.0 0.0 0.0 2.0840392685602716 0.0 48-49 0.0 0.0 0.0 2.397889184558916 0.0 50-51 0.0 0.0 0.0 2.726311973214349 0.0 52-53 0.0 0.0 0.0 3.0196650845819004 0.0 54-55 0.0 0.0 0.0 3.327946504524699 0.0 56-57 0.0 0.0 0.0 3.6955857228500273 0.0 58-59 0.0 0.0 0.0 4.0648836421281604 0.0 60-61 0.0 0.0 0.0 4.429560894466337 0.0 62-63 0.0 0.0 0.0 4.797910984628581 0.0 64-65 0.0 0.0 0.0 5.19398507643057 0.0 66-67 0.0 0.0 0.0 5.62666906783376 0.0 68-69 0.0 0.0 0.0 6.11266844700569 0.0 70-71 0.0 0.0 0.0 6.638002734486999 0.0 72-73 0.0 0.0 0.0 7.162981586049851 0.0 74-75 0.0 0.0 0.0 7.694476762784437 0.0 76-77 0.0 0.0 0.0 8.250852453811103 0.0 78-79 0.0 0.0 0.0 8.860662011245992 0.0 80-81 0.0 0.0 0.0 9.505185810050307 0.0 82-83 0.0 0.0 0.0 10.188689081245542 0.0 84-85 0.0 0.0 0.0 10.876576062101762 0.0 86-87 0.0 0.0 0.0 11.580813095207066 0.0 88 0.0 0.0 0.0 12.138373572628591 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACAAA 455 0.0 37.29055 1 GAGAACA 710 0.0 32.527107 1 CCCTAGA 145 2.5807822E-8 29.253794 1 ACAACAC 670 0.0 27.353168 5 TAACAAC 260 0.0 27.191025 1 GTATAAG 125 7.836728E-6 26.393421 1 TTGTACG 45 9.572068E-4 26.08464 40-41 GTACAAG 275 0.0 25.70788 1 GTACATG 2650 0.0 25.255161 1 TACATGG 2620 0.0 24.045149 2 GTACCCA 180 2.0969674E-7 23.565556 1 ATAATAC 160 2.036968E-6 23.49567 3 GTATCAA 2505 0.0 22.765926 1 CGTATCA 85 3.2952903E-6 22.234901 92-93 GTACATA 150 3.2150987E-5 21.994518 1 AACAACA 1035 0.0 21.793085 4 CATGGGG 1040 0.0 21.688309 4 GGTATCA 1945 0.0 21.566422 1 GGAAACA 700 0.0 21.54565 1 ACATGGG 2735 0.0 21.30503 3 >>END_MODULE