FastQCFastQC Report
Wed 25 May 2016
SRR1033120_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033120_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences422017
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50121.1876298822085365No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47801.1326557934869923No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27790.6585042782636719No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA25130.5954736420570735No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT19860.47059715603873775No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14540.34453588362554116No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13900.329370617771322No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11460.27155304170211153No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9600.22747898781328713No Hit
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT8380.19857019977868193RNA PCR Primer, Index 39 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCT5590.13245911894544532RNA PCR Primer, Index 39 (96% over 25bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26100.045.8013571
GTATCAA41750.034.6072431
ACGCAGT550.002716009334.1794058
GTATAGG600.00413059231.3757321
GTATTAG1354.5686284E-727.889541
GTTGTAT856.7552645E-427.684471
ATCAACG52100.027.6059283
TATCAAC52550.027.2800882
TCAACGC53100.027.2630774
CAACGCA53650.027.0711925
AACGCAG54400.026.7811996
GTGTAGC1458.527495E-725.9661241
TATATCC1101.0165098E-425.637593
AGAGCGG1101.0165098E-425.637595
CTATAGT950.001302854524.7380284
CGCAGAG59300.024.3304928
ACGCAGA59700.024.3249157
GTCTAGG1000.001743070823.53181
CCCTTAC1000.001743070823.53181
CGTAGCT500.001759016423.4983416-17
Produced by FastQC (version 0.11.5)