FastQCFastQC Report
Wed 25 May 2016
SRR1033119_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033119_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2534783
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19300.037.5967451
GTATCAA33500.034.8813821
TCAACGC33200.033.810844
ATCAACG33400.033.4737053
CAACGCA35750.031.5299135
AACGCAG36800.030.1132416
TATCAAC40000.028.2054622
GTACATG71350.028.0660781
TACATGG73350.025.891862
ACGCAGA43200.025.5433257
CGCAGAG44350.024.5633548
ACATGGG75850.024.0916923
GTATAGA10450.020.7409341
CATGGGG50550.019.9762274
TAGACTG9450.019.3829735
CTGTCGC6300.018.6334299
GCAGAGT56350.018.4991769
CGTATAC1700.001926143416.579663
GAGTACA54400.016.5432661
TATACTG15200.016.3764535
Produced by FastQC (version 0.11.5)