##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033119_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2534783
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.707498038293615	33.0	31.0	34.0	30.0	34.0
2	31.783602383320385	34.0	31.0	34.0	30.0	34.0
3	31.960685786515057	34.0	31.0	34.0	30.0	34.0
4	35.31786468506377	37.0	35.0	37.0	33.0	37.0
5	35.408289388085684	37.0	35.0	37.0	35.0	37.0
6	35.47048051056047	37.0	37.0	37.0	35.0	37.0
7	35.43625785718146	37.0	37.0	37.0	35.0	37.0
8	35.42534370792293	37.0	37.0	37.0	35.0	37.0
9	37.09560818421143	39.0	38.0	39.0	35.0	39.0
10-11	37.09315471975313	39.0	38.0	39.0	35.0	39.0
12-13	37.04552007015985	39.0	38.0	39.0	35.0	39.0
14-15	38.523607938036506	41.0	39.0	41.0	35.0	41.0
16-17	38.54025374164179	41.0	39.0	41.0	35.0	41.0
18-19	38.53338845968274	41.0	39.0	41.0	35.0	41.0
20-21	38.571737107279006	41.0	39.0	41.0	35.0	41.0
22-23	38.54514469285931	41.0	39.0	41.0	34.0	41.0
24-25	38.54017365589086	41.0	39.0	41.0	34.0	41.0
26-27	38.505618429664395	41.0	39.0	41.0	34.0	41.0
28-29	38.47898459158043	40.5	39.0	41.0	34.0	41.0
30-31	38.48104650378356	41.0	39.0	41.0	34.0	41.0
32-33	38.54638266865447	41.0	39.0	41.0	34.0	41.0
34-35	38.51738985151786	41.0	39.0	41.0	34.0	41.0
36-37	38.44061523215202	41.0	39.0	41.0	34.0	41.0
38-39	38.36369247387252	41.0	39.0	41.0	34.0	41.0
40-41	38.30740737964551	41.0	39.0	41.0	34.0	41.0
42-43	38.20124917991008	41.0	38.5	41.0	33.0	41.0
44-45	38.075458135864096	40.0	38.0	41.0	33.0	41.0
46-47	37.958255203699885	40.0	38.0	41.0	33.0	41.0
48-49	37.84530470655673	40.0	38.0	41.0	33.0	41.0
50-51	37.73283117331937	40.0	38.0	41.0	33.0	41.0
52-53	37.59633132303633	40.0	37.5	41.0	32.0	41.0
54-55	37.452225890737	40.0	37.0	41.0	32.0	41.0
56-57	37.30440870086315	40.0	37.0	41.0	32.0	41.0
58-59	37.118038506649285	40.0	36.0	41.0	31.0	41.0
60-61	36.9048202548305	39.5	36.0	41.0	31.0	41.0
62-63	36.64590874248407	39.0	35.0	41.0	31.0	41.0
64-65	36.37937192256694	39.0	35.0	41.0	31.0	41.0
66-67	36.0900213154341	38.5	35.0	40.5	30.5	41.0
68-69	35.781986071391515	38.0	35.0	40.0	30.0	41.0
70-71	35.43216164855137	37.0	35.0	40.0	30.0	41.0
72-73	35.06738880606348	37.0	35.0	39.5	29.5	41.0
74-75	34.696741890725946	36.0	34.0	39.0	29.0	41.0
76-77	34.36085100775885	36.0	34.0	39.0	29.0	40.0
78-79	33.989495353251144	35.0	34.0	37.5	29.0	39.5
80-81	33.62480594985843	35.0	34.0	37.0	29.0	39.0
82-83	33.396755264651844	35.0	34.0	37.0	29.0	39.0
84-85	33.115647374942945	35.0	34.0	36.0	29.0	37.5
86-87	32.85710019358659	35.0	34.0	36.0	29.0	37.0
88-89	32.484361777714305	35.0	34.0	35.5	27.5	37.0
90-91	32.29077380588397	35.0	34.0	35.0	27.0	36.0
92-93	32.10999166398071	35.0	34.0	35.0	27.0	36.0
94-95	31.939367196324106	35.0	34.0	35.0	27.0	36.0
96-97	31.76489545653415	35.0	33.5	35.0	26.5	36.0
98-99	31.576633778907308	35.0	33.0	35.0	25.5	35.5
100	31.417582491282293	35.0	33.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.20342120000875852
1101	2	0.23148119029534797
1101	3	0.2733867596419124
1101	4	0.2720895963955243
1101	5	0.24535508655694827
1101	6	0.2625394835013566
1101	7	0.2751046293756687
1101	8	0.24188902726772454
1101	9	0.3690082982150713
1101	10-11	0.3343521310068738
1101	12-13	0.316686852961638
1101	14-15	0.39209341023011035
1101	16-17	0.3510993740628905
1101	18-19	0.3415351048922872
1101	20-21	0.27080102670287687
1101	22-23	0.21821729513555255
1101	24-25	0.25781392666093694
1101	26-27	0.23160186932511806
1101	28-29	0.242833350291896
1101	30-31	0.26043955394704454
1101	32-33	0.26863534307074133
1101	34-35	0.27460679812541855
1101	36-37	0.29923517514427544
1101	38-39	0.3068980365492564
1101	40-41	0.2759849904465099
1101	42-43	0.16741240273445612
1101	44-45	0.19790038027068846
1101	46-47	0.19245581328183192
1101	48-49	0.24052878145543843
1101	50-51	0.21845692490244062
1101	52-53	0.16806484240169084
1101	54-55	0.11990403058057097
1101	56-57	0.11409645963692583
1101	58-59	0.1900564344513569
1101	60-61	0.13497357229223184
1101	62-63	0.1565277491885766
1101	64-65	0.17375427818677736
1101	66-67	0.15300134006388078
1101	68-69	0.1311090203368579
1101	70-71	0.15542753438478485
1101	72-73	0.19863016383137477
1101	74-75	0.24952286470011842
1101	76-77	0.1874991010475
1101	78-79	0.15469065825419648
1101	80-81	0.26884337019664883
1101	82-83	0.1929310991969686
1101	84-85	0.1653373430466587
1101	86-87	0.1796561455836425
1101	88-89	0.39977511790678477
1101	90-91	0.37477384214565035
1101	92-93	0.3952634949578666
1101	94-95	0.40294211697345617
1101	96-97	0.314667723881648
1101	98-99	0.35805880509428434
1101	100	0.33746061627739365
1102	1	0.13652903637916936
1102	2	0.12770093619093004
1102	3	0.1346425547252572
1102	4	0.11059953865445493
1102	5	0.05886969831650646
1102	6	0.04395405125026741
1102	7	0.010535626694199607
1102	8	-0.013501392813736857
1102	9	0.1259489764291004
1102	10-11	0.09125403066581583
1102	12-13	0.06683023315285652
1102	14-15	0.08269414751291748
1102	16-17	0.07509560020624662
1102	18-19	0.03503149515130133
1102	20-21	0.05398309286287173
1102	22-23	0.028948169230694987
1102	24-25	0.08770389843818549
1102	26-27	0.12988270546953373
1102	28-29	0.09503758765876569
1102	30-31	0.13062357888619403
1102	32-33	0.08402619393572763
1102	34-35	0.113923454885132
1102	36-37	0.12325467645767674
1102	38-39	0.01676560758273382
1102	40-41	0.055776002565998795
1102	42-43	0.02233985910878289
1102	44-45	-0.0070952781385429375
1102	46-47	-0.018499847840693917
1102	48-49	-0.0621938588875679
1102	50-51	-0.01710933523101943
1102	52-53	-0.07009901833178134
1102	54-55	-0.11778785696177607
1102	56-57	-0.04840380960278168
1102	58-59	-0.0767425159799231
1102	60-61	-0.06367968264707002
1102	62-63	-0.09538461625988504
1102	64-65	-0.03024856884231042
1102	66-67	-0.08530489623503712
1102	68-69	-0.05568056858955828
1102	70-71	0.0025855364621918397
1102	72-73	8.9710592087755E-4
1102	74-75	-0.10074011418087281
1102	76-77	-0.08475368144443252
1102	78-79	-0.11479839871885389
1102	80-81	-0.08261848890434464
1102	82-83	-0.08501714794805082
1102	84-85	-0.11400898151786976
1102	86-87	-0.07570859626289916
1102	88-89	0.07170219268405731
1102	90-91	0.0960366020986001
1102	92-93	0.0667398946105422
1102	94-95	0.07857642161650347
1102	96-97	0.06848160482015331
1102	98-99	0.10736509090115831
1102	100	0.08227956020834526
1103	1	0.17503676431532966
1103	2	0.1113409538632979
1103	3	0.22229783608447562
1103	4	0.23081783837499614
1103	5	0.13169231298898154
1103	6	0.13032892407098728
1103	7	0.11425065879600993
1103	8	0.13644247288370082
1103	9	0.19831255035856543
1103	10-11	0.17556325895266411
1103	12-13	0.2390818955261409
1103	14-15	0.2554118363692979
1103	16-17	0.24263441064958613
1103	18-19	0.2207234885927818
1103	20-21	0.22856566384498223
1103	22-23	0.2817534484010551
1103	24-25	0.22787607209313165
1103	26-27	0.2887588489187536
1103	28-29	0.3057638733221282
1103	30-31	0.2985558029253994
1103	32-33	0.31575972551919307
1103	34-35	0.30763721247745934
1103	36-37	0.2651253940049543
1103	38-39	0.2912201609967866
1103	40-41	0.2857552595751045
1103	42-43	0.23376786236381975
1103	44-45	0.19328268144786875
1103	46-47	0.2416317262452523
1103	48-49	0.20397754726739237
1103	50-51	0.15846482274668006
1103	52-53	0.04964778274259629
1103	54-55	0.09484332359414793
1103	56-57	0.07916360511762832
1103	58-59	0.10195993402778925
1103	60-61	0.13725209088951829
1103	62-63	0.18505772545954358
1103	64-65	0.1502769264699424
1103	66-67	0.20492978426981523
1103	68-69	0.22186327313902865
1103	70-71	0.2183648019783817
1103	72-73	0.16219043186899285
1103	74-75	0.14448555937642027
1103	76-77	0.1113954991903583
1103	78-79	0.12709608586857968
1103	80-81	0.11535276679330053
1103	82-83	0.09902756917139044
1103	84-85	0.12791519246493976
1103	86-87	0.08911834987890899
1103	88-89	0.18525004205411477
1103	90-91	0.2835050304343767
1103	92-93	0.1647430363307123
1103	94-95	0.11879208538444885
1103	96-97	0.0451625058378049
1103	98-99	0.1914871013536832
1103	100	0.24150662066790574
1104	1	0.2196250747353119
1104	2	0.2022940364609127
1104	3	0.17985425718508807
1104	4	0.29051047222034043
1104	5	0.18050144000392976
1104	6	0.18037895026265005
1104	7	0.24156116998045007
1104	8	0.18518114894563809
1104	9	0.27599927489526266
1104	10-11	0.21727003516893006
1104	12-13	0.24168312967344718
1104	14-15	0.19225785772879078
1104	16-17	0.23279067567539613
1104	18-19	0.27584041950565563
1104	20-21	0.30461615184000834
1104	22-23	0.2710923470338642
1104	24-25	0.197871672855932
1104	26-27	0.20112260019666195
1104	28-29	0.31653993681009496
1104	30-31	0.27865198013036974
1104	32-33	0.23805969274484085
1104	34-35	0.20882772080176437
1104	36-37	0.26474577088673357
1104	38-39	0.18831436369538324
1104	40-41	0.2215619226112011
1104	42-43	0.2538962710677737
1104	44-45	0.2193730678401451
1104	46-47	0.20100201502574322
1104	48-49	0.14721870313520213
1104	50-51	0.12176085366630218
1104	52-53	0.11548995020187647
1104	54-55	0.05581248058796717
1104	56-57	0.08753997531159996
1104	58-59	0.015572328824106307
1104	60-61	0.137758856549425
1104	62-63	0.17145430056578448
1104	64-65	0.040583100898231805
1104	66-67	0.10219669330680148
1104	68-69	0.13693949888974544
1104	70-71	0.205791186559523
1104	72-73	0.16997567571011274
1104	74-75	0.13904794128930575
1104	76-77	0.07051766828572426
1104	78-79	0.10006081460272043
1104	80-81	0.13828083020487725
1104	82-83	0.12993835713710666
1104	84-85	0.04197762373860314
1104	86-87	0.061767966612940484
1104	88-89	0.16406851566241443
1104	90-91	0.18320542967398978
1104	92-93	0.13982591770402308
1104	94-95	0.14614780698303775
1104	96-97	0.14171275499884217
1104	98-99	0.20255455509609277
1104	100	0.2440117272341844
1105	1	0.0370842493211363
1105	2	-0.012949728764393598
1105	3	0.029204283024597544
1105	4	0.018621706615164157
1105	5	-0.009653085752240997
1105	6	-0.009874586023734366
1105	7	-0.046593631936381996
1105	8	-0.00355270930479179
1105	9	0.08405512116736702
1105	10-11	0.026947530934805286
1105	12-13	0.021609513791560175
1105	14-15	0.07448494036582787
1105	16-17	0.06053430729787124
1105	18-19	0.030952388788278995
1105	20-21	0.11907478739257016
1105	22-23	0.12610879390340557
1105	24-25	0.16176792715252475
1105	26-27	0.12858637360857017
1105	28-29	0.14117546106616174
1105	30-31	0.18529541338514122
1105	32-33	0.1692565097909977
1105	34-35	0.11294486896341027
1105	36-37	0.10665151921712379
1105	38-39	0.09194584751297441
1105	40-41	0.06690635504182296
1105	42-43	0.08382705324662254
1105	44-45	0.05302714425749144
1105	46-47	0.0775383031433492
1105	48-49	0.06893426891936372
1105	50-51	0.11480816235643942
1105	52-53	0.11283973945604941
1105	54-55	0.12092060644212665
1105	56-57	0.10070223732162731
1105	58-59	0.028114240393186662
1105	60-61	0.07199708787326387
1105	62-63	0.09797534351714177
1105	64-65	0.10089044373869172
1105	66-67	0.09201254671980053
1105	68-69	0.1047253648946267
1105	70-71	0.10893455062969792
1105	72-73	0.20740497814735193
1105	74-75	0.13465013330012
1105	76-77	0.1359082738546391
1105	78-79	0.14465018160839094
1105	80-81	0.13119962741541968
1105	82-83	0.07209696146271938
1105	84-85	0.10016385915170645
1105	86-87	0.15779828708929955
1105	88-89	0.1842732717498663
1105	90-91	0.18580169376330957
1105	92-93	0.20640064155058013
1105	94-95	0.30911444556181067
1105	96-97	0.28843783908196485
1105	98-99	0.28638130414491414
1105	100	0.3221459846465393
1106	1	0.09709275497684544
1106	2	0.06292983336003388
1106	3	0.08692537484149554
1106	4	0.08616489767207725
1106	5	0.14087575615302939
1106	6	0.032071552187645125
1106	7	0.006842045423113063
1106	8	0.036331635870176626
1106	9	-0.03266732934518046
1106	10-11	-0.016898710007218654
1106	12-13	-0.024738301249094263
1106	14-15	-0.009968488407594123
1106	16-17	-0.03650832039742369
1106	18-19	-0.009552482632720682
1106	20-21	-0.08914076470180277
1106	22-23	-0.02174974157732379
1106	24-25	-0.04860621123560094
1106	26-27	0.007375118527278346
1106	28-29	0.00516864549528151
1106	30-31	-0.0029688529192029023
1106	32-33	1.8677297191516118E-5
1106	34-35	-0.04102724463680829
1106	36-37	0.01200359260878514
1106	38-39	-0.09950237845026777
1106	40-41	-0.12109349030989591
1106	42-43	-0.06081258863213179
1106	44-45	-0.04051297592631187
1106	46-47	-0.05514785473045691
1106	48-49	-0.055511344592488854
1106	50-51	-0.08182727087968544
1106	52-53	-0.1512564517775985
1106	54-55	-0.07067177381591705
1106	56-57	-0.04454149942280594
1106	58-59	-0.053590038945806384
1106	60-61	-0.14274089374568177
1106	62-63	-0.09112816589431105
1106	64-65	-0.10372898339986847
1106	66-67	-0.16854786000518374
1106	68-69	-0.17407787830084231
1106	70-71	-0.1715100355756789
1106	72-73	-0.10620269019835149
1106	74-75	-0.09143102391091418
1106	76-77	-0.2253236102033327
1106	78-79	-0.1234816394856395
1106	80-81	-0.151570758542519
1106	82-83	-0.16024841786743593
1106	84-85	-0.2197788657510884
1106	86-87	-0.1337359619828078
1106	88-89	-0.07336732011121683
1106	90-91	-0.0614133943862214
1106	92-93	-0.034647431973631626
1106	94-95	-0.03685158152372736
1106	96-97	-0.018931980200470377
1106	98-99	-0.007265550971734314
1106	100	0.05288674993462905
1107	1	0.32367298209563344
1107	2	0.27796037956695585
1107	3	0.2988448117011977
1107	4	0.3149520187879844
1107	5	0.25998367197302485
1107	6	0.2305446387171557
1107	7	0.2556551883618994
1107	8	0.2123504160327414
1107	9	0.2933353112017656
1107	10-11	0.2778945110896345
1107	12-13	0.3025154902376528
1107	14-15	0.33274857619320386
1107	16-17	0.27947075895656326
1107	18-19	0.3743036022669486
1107	20-21	0.28242933546964366
1107	22-23	0.31932971427950463
1107	24-25	0.28203696567575065
1107	26-27	0.3285746028533225
1107	28-29	0.26360774622765604
1107	30-31	0.30457764108003715
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2216	18-19	-0.36391342839991836
2216	20-21	-0.3813027741815276
2216	22-23	-0.36351492566021903
2216	24-25	-0.3776268241103864
2216	26-27	-0.44301964007060946
2216	28-29	-0.39357060715021674
2216	30-31	-0.43492638709027887
2216	32-33	-0.3702807329761839
2216	34-35	-0.3840958899189957
2216	36-37	-0.3111962255129086
2216	38-39	-0.35676235419982305
2216	40-41	-0.3553444823733827
2216	42-43	-0.362244880298789
2216	44-45	-0.27486041860709776
2216	46-47	-0.27778688140594454
2216	48-49	-0.23912920724680475
2216	50-51	-0.23846100654988334
2216	52-53	-0.26466584331198817
2216	54-55	-0.24199976102931942
2216	56-57	-0.3556154081529712
2216	58-59	-0.40833127571828953
2216	60-61	-0.31638507363986434
2216	62-63	-0.24321769962446638
2216	64-65	-0.2236384576062136
2216	66-67	-0.24069085493464115
2216	68-69	-0.2513210440856781
2216	70-71	-0.34251629108713644
2216	72-73	-0.26654081850523426
2216	74-75	-0.24398696526893815
2216	76-77	-0.27741796646434835
2216	78-79	-0.30087843732513875
2216	80-81	-0.22625591617038765
2216	82-83	-0.22295303096267105
2216	84-85	-0.2642384667211104
2216	86-87	-0.29329077349074595
2216	88-89	-0.2813665209489642
2216	90-91	-0.27114319487122174
2216	92-93	-0.2605733578995597
2216	94-95	-0.23505277751138465
2216	96-97	-0.2397914929870666
2216	98-99	-0.3156436649488086
2216	100	-0.26134936117648166
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	22692.0
3	4021.0
4	2663.0
5	2262.0
6	1871.0
7	2109.0
8	2582.0
9	2794.0
10	2936.0
11	3254.0
12	3635.0
13	4266.0
14	4829.0
15	5153.0
16	5088.0
17	5132.0
18	5036.0
19	4901.0
20	4890.0
21	5128.0
22	5960.0
23	8095.0
24	12804.0
25	18089.0
26	18607.0
27	15455.0
28	16359.0
29	20645.0
30	25258.0
31	30700.0
32	40799.0
33	56110.0
34	79097.0
35	120731.0
36	226576.0
37	492528.0
38	848187.0
39	402675.0
40	866.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.60756289980957	20.20583772931717	13.664843357575071	23.52175601329819
2	15.041208845465052	20.517367910897573	40.960834019949935	23.480589223687442
3	17.879930915371332	25.977596841845546	30.20857636318776	25.933895879595358
4	11.983218781388318	17.15595443180854	39.80077236229593	31.06005442450721
5	12.555644445461834	38.405294031979054	36.05262773604513	12.986433786513985
6	30.088018979178692	38.90550141306188	18.23984136146332	12.766638246296115
7	26.745949989406707	32.87980105917133	23.064021749980174	17.31022720144179
8	24.43000405618047	37.279573422390435	20.706880197474437	17.583542323954664
9	24.599067212765455	17.55995643836265	21.488198110750726	36.35277823812117
10-11	23.360570674112346	27.510490216891203	29.342144826154794	19.78679428284166
12-13	24.107324641217424	24.814847987929312	29.25806225053306	21.8197651203202
14-15	21.400666053749905	26.8831407117463	26.199015124312936	25.51717811019086
16-17	21.183523430798235	30.13636037207702	26.772274469540868	21.907841727583875
18-19	21.078658548495067	29.190477346043675	28.650410199681215	21.080453905780043
20-21	22.28363788178388	28.417769945335568	28.138311704454562	21.16028046842599
22-23	21.88145183612166	28.237777355587873	28.17963746513234	21.701133343158126
24-25	21.787824242248462	28.378059225577246	28.066515965753524	21.767600566420768
26-27	21.49809030596733	28.384471254759436	28.14225509029788	21.97518334897535
28-29	21.580208897055037	28.301899076691665	28.248748525486516	21.86914350076678
30-31	21.78959392966077	28.718931140477217	27.91432708963852	21.577147840223496
32-33	21.414655917063214	28.41392707911187	28.424305226442886	21.74711177738203
34-35	21.552153060962787	28.19522453899618	28.41689305026998	21.835729349771054
36-37	21.905659405878872	28.5567401399183	27.859358099197472	21.678242355005356
38-39	21.72326745252273	28.649077758084907	28.04483056852777	21.582824220864588
40-41	21.436317169858032	28.333101538819662	28.344977308796647	21.88560398252565
42-43	21.46912727846603	28.271679949498935	28.32123411978221	21.93795865225282
44-45	21.57772054251454	28.412356661963695	28.27894142118105	21.730981374340715
46-47	21.629332787813066	28.57457645408013	28.110575169450918	21.68551558865589
48-49	21.577910071723185	28.43363973021399	28.143346160090317	21.84510403797251
50-51	21.57809475638615	28.3376719089917	28.153325184859618	21.93090814976253
52-53	21.612629273994287	28.332085595782353	28.132329225169777	21.922955905053584
54-55	21.69940713080306	28.698676277243035	28.017611499551727	21.584305092402182
56-57	21.567855813460795	28.387869018093344	28.00770815986719	22.036567008578672
58-59	21.415580419138998	28.04368924378235	28.38970379371292	22.151026543365738
60-61	21.74814613754326	28.458820503190115	27.99922121206861	21.79381214719802
62-63	21.57097678793206	28.44052351427142	28.02300139345	21.96549830434652
64-65	21.520565174405714	28.230965762714806	28.196484663801396	22.051984399078083
66-67	21.445244021924918	28.284698853906797	28.062447393550784	22.2076097306175
68-69	21.872441759402786	28.457478811110263	27.9221493975029	21.747930031984048
70-71	21.694423507443915	28.262169339775333	28.10351279361965	21.939894359161098
72-73	21.55200234186494	28.34830270024602	28.178383355715713	21.921311602173326
74-75	21.625173909775818	28.464392213813007	28.023376945654142	21.887056930757034
76-77	21.63823279641533	28.396298155686168	27.947760715592583	22.01770833230592
78-79	21.695574846963655	28.514174503253255	28.011647963539556	21.778602686243538
80-81	21.689978533855633	28.312921230178535	28.07713293425742	21.919967301708414
82-83	21.539237531936518	28.32642789172436	28.237384385469618	21.89695019086951
84-85	21.563394584597127	28.275027853438832	28.10037180001073	22.061205761953307
86-87	21.773741121293273	28.392885041164373	27.954216567063135	21.87915727047922
88-89	21.57968844421556	28.307896259207112	28.030395492671524	22.08201980390581
90-91	21.52299078137873	28.250994514317966	28.114621558597403	22.1113931457059
92-93	21.693601797488064	28.42120221403342	27.918918538074266	21.966277450404252
94-95	21.513841681893734	28.19359621786639	28.111481182202507	22.18108091803737
96-97	21.539258033067103	28.16510096759746	28.128032981776162	22.16760801755927
98-99	21.59560402665253	28.264749764908213	28.010122390600216	22.129523817839036
100	21.708324734494308	28.190354483831538	27.890281459844747	22.21103932182941
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	368.0
1	357.5
2	348.5
3	308.5
4	249.0
5	231.0
6	210.0
7	190.0
8	191.0
9	200.0
10	195.0
11	184.5
12	203.5
13	228.5
14	259.0
15	322.0
16	417.0
17	579.0
18	852.5
19	1235.0
20	1849.0
21	2714.0
22	4078.0
23	6389.5
24	9170.5
25	12325.5
26	16749.5
27	22724.5
28	29787.5
29	37675.5
30	45118.5
31	52703.0
32	60536.0
33	67719.5
34	74783.0
35	80571.5
36	85024.5
37	89543.5
38	92370.0
39	93065.0
40	94008.0
41	93991.5
42	92576.5
43	91285.0
44	88947.0
45	86385.5
46	84730.0
47	81984.0
48	79110.5
49	77232.5
50	75067.5
51	71967.0
52	67111.5
53	63134.0
54	60739.5
55	57687.0
56	53259.5
57	48262.5
58	43860.5
59	39867.5
60	35525.5
61	30318.5
62	26055.0
63	22247.5
64	18140.0
65	14523.5
66	11410.5
67	8462.5
68	6316.0
69	4950.5
70	3574.5
71	2569.5
72	2006.0
73	1529.5
74	1187.0
75	902.0
76	619.5
77	462.5
78	323.5
79	195.5
80	130.5
81	82.5
82	54.5
83	36.5
84	26.5
85	17.5
86	14.5
87	11.0
88	6.5
89	5.0
90	4.5
91	4.5
92	4.0
93	3.0
94	1.5
95	1.5
96	2.5
97	2.0
98	0.0
99	1.0
100	2.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.33233614080574153
2	0.12012862639523778
3	0.06540993844443489
4	0.03937220661492522
5	0.04189707758021101
6	0.008521439507839526
7	0.006312177413214464
8	0.014951970247551762
9	0.01747684121283755
10-11	0.02039622326644924
12-13	0.014951970247551762
14-15	0.017871352301163455
16-17	0.0185025700424849
18-19	0.0184828444880686
20-21	0.0234339586465587
22-23	0.014932244693135468
24-25	0.02463721746595271
26-27	0.018798453358729327
28-29	0.018640648923398964
30-31	0.01944939665446707
32-33	0.02414407860554533
34-35	0.014518008050393268
36-37	0.009172382803577269
38-39	0.012111490411605254
40-41	0.00830445840926028
42-43	0.007219552916364043
44-45	0.008481988399006936
46-47	0.007870496212101785
48-49	0.004240994199503468
50-51	0.006193824086716694
52-53	0.007712691776771424
54-55	0.0029785587168605753
56-57	0.002623498737367262
58-59	0.0024854198564531953
60-61	0.0029588331624442804
62-63	0.002643224291783557
64-65	0.0024065176387880146
66-67	0.0016372210165525017
68-69	0.0032349909242724135
70-71	0.0024656943020369003
72-73	0.0020120065504621106
74-75	0.0025051454108694906
76-77	0.0039648364376753354
78-79	0.0035900509037657268
80-81	0.003708404230263498
82-83	0.002643224291783557
84-85	0.0034322464684353655
86-87	0.002643224291783557
88-89	0.6048052239580272
90-91	0.6149836100368354
92-93	0.611077950262409
94-95	0.5975659454872468
96-97	0.5952580556205402
98-99	0.5926542824375893
100	0.5942520523453092
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2534783.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.480856492239
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.16204136838914	51.18117413983937
2	13.0495291584166	17.08988692227131
3	4.0910225587228055	8.036509832227212
4	1.7397447057789184	4.556798936489677
5	0.8829112957176422	2.8906893925131856
6	0.5231423545632171	2.055348566649962
7	0.3359804534152644	1.5400201497997619
8	0.23414810609180625	1.226577482634169
9	0.1727942455068132	1.018324367344471
>10	0.7738603047881422	8.597579705429112
>50	0.02961431749708477	1.2805529176600803
>100	0.005150805762633158	0.5040612018785614
>500	6.0325349939989633E-5	0.022476385263085343
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	3.9451108832590406E-5	0.0
4	0.0	0.0	0.0	3.9451108832590406E-5	0.0
5	0.0	0.0	0.0	7.890221766518081E-5	0.0
6	0.0	0.0	0.0	7.890221766518081E-5	0.0
7	0.0	0.0	0.0	7.890221766518081E-5	0.0
8	0.0	0.0	0.0	7.890221766518081E-5	0.0
9	0.0	0.0	0.0	7.890221766518081E-5	0.0
10-11	0.0	0.0	0.0	9.862777208147601E-5	0.0
12-13	0.0	0.0	0.0	1.380788809140664E-4	0.0
14-15	0.0	0.0	0.0	1.9725554416295202E-4	0.0
16-17	0.0	0.0	0.0	1.9725554416295202E-4	0.0
18-19	0.0	1.9725554416295203E-5	0.0	2.3670665299554242E-4	0.0
20-21	0.0	5.917666324888561E-5	0.0	3.156088706607232E-4	0.0
22-23	0.0	7.890221766518081E-5	0.0	4.5368775157478964E-4	0.0
24-25	0.0	7.890221766518081E-5	0.0	5.325899692399704E-4	0.0
26-27	0.0	7.890221766518081E-5	0.0	7.890221766518081E-4	0.0
28-29	0.0	7.890221766518081E-5	0.0	0.0015188676900547305	0.0
30-31	0.0	7.890221766518081E-5	0.0	0.005069467484987867	0.0
32-33	0.0	7.890221766518081E-5	0.0	0.01327529812216667	0.0
34-35	0.0	7.890221766518081E-5	0.0	0.023650939745137947	0.0
36-37	0.0	7.890221766518081E-5	0.0	0.04414579078366866	0.0
38-39	0.0	7.890221766518081E-5	0.0	0.07803429327086381	0.0
40-41	0.0	7.890221766518081E-5	0.0	0.1195763108715815	0.0
42-43	0.0	7.890221766518081E-5	0.0	0.1628344517065169	0.0
44-45	0.0	9.862777208147601E-5	0.0	0.20986017343496466	0.0
46-47	0.0	1.1835332649777121E-4	0.0	0.26816891228953327	0.0
48-49	0.0	1.1835332649777121E-4	0.0	0.3361826239169191	0.0
50-51	0.0	1.5780443533036163E-4	0.0	0.4073326986964959	0.0
52-53	0.0	2.3670665299554242E-4	0.0	0.48321690653598354	0.0
54-55	0.0	2.564322074118376E-4	0.0	0.5625530863983228	0.0
56-57	0.0	2.761577618281328E-4	0.0	0.6560916654403948	0.0
58-59	0.0	2.761577618281328E-4	0.0	0.7650161769271768	0.0
60-61	0.0	3.1560887066072325E-4	0.0	0.881436399092151	0.0
62-63	0.0	3.1560887066072325E-4	0.0	1.0013283188343933	0.0
64-65	0.0	3.1560887066072325E-4	0.0	1.125599311657053	0.0
66-67	0.0	3.1560887066072325E-4	0.0	1.2675838523455458	0.0
68-69	0.0	3.1560887066072325E-4	0.0	1.4339886294014121	0.0
70-71	0.0	3.1560887066072325E-4	0.0	1.6133530957087845	0.0
72-73	0.0	3.1560887066072325E-4	0.0	1.798142089480638	0.0
74-75	0.0	3.1560887066072325E-4	0.0	1.984252695398383	0.0
76-77	0.0	3.1560887066072325E-4	0.0	2.1804627851772715	0.0
78-79	0.0	3.1560887066072325E-4	0.0	2.412810090646813	0.0
80-81	0.0	3.1560887066072325E-4	0.0	2.6695776324837275	0.0
82-83	0.0	3.1560887066072325E-4	0.0	2.9321839384278654	0.0
84-85	0.0	3.3533442507701846E-4	0.0	3.1871170037040644	0.0
86-87	0.0	3.550599794933136E-4	0.0	3.454753326024358	0.0
88	0.0	3.550599794933136E-4	0.0	3.680670100754187	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1930	0.0	37.596745	1
GTATCAA	3350	0.0	34.881382	1
TCAACGC	3320	0.0	33.81084	4
ATCAACG	3340	0.0	33.473705	3
CAACGCA	3575	0.0	31.529913	5
AACGCAG	3680	0.0	30.113241	6
TATCAAC	4000	0.0	28.205462	2
GTACATG	7135	0.0	28.066078	1
TACATGG	7335	0.0	25.89186	2
ACGCAGA	4320	0.0	25.543325	7
CGCAGAG	4435	0.0	24.563354	8
ACATGGG	7585	0.0	24.091692	3
GTATAGA	1045	0.0	20.740934	1
CATGGGG	5055	0.0	19.976227	4
TAGACTG	945	0.0	19.382973	5
CTGTCGC	630	0.0	18.633429	9
GCAGAGT	5635	0.0	18.499176	9
CGTATAC	170	0.0019261434	16.57966	3
GAGTACA	5440	0.0	16.543266	1
TATACTG	1520	0.0	16.376453	5
>>END_MODULE