FastQCFastQC Report
Wed 25 May 2016
SRR1033119_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033119_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2534783
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54060.21327269434898372No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42950.16944251243597577No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA34550.054.342311
GTATCAA69100.045.9662441
ATCAACG81750.038.2960433
TCAACGC82200.038.1435784
CAACGCA85200.036.7460445
TATCAAC87400.035.9831542
AACGCAG89250.035.1208536
ACGCAGA102100.030.5625657
CGCAGAG103950.030.0174588
GCAGAGT116000.026.5751769
GTACATG69550.026.0481471
TACATGG69900.025.0178682
ACATGGG70250.024.0223543
GTGGTAT15950.023.8966831
TGGTATC15650.022.8288382
AGAGTAC106500.021.46661610-11
CAGAGTA113350.021.3923510-11
GAGTACT81750.021.26844412-13
CATGGGG49700.020.05154
AGTACTT84800.019.81079712-13
Produced by FastQC (version 0.11.5)