##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1033119_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2534783 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.787560118558474 34.0 31.0 34.0 31.0 34.0 2 32.880931424899096 34.0 31.0 34.0 31.0 34.0 3 32.96523055425257 34.0 31.0 34.0 31.0 34.0 4 36.385636561394016 37.0 37.0 37.0 35.0 37.0 5 36.32682837150163 37.0 37.0 37.0 35.0 37.0 6 36.411593023939325 37.0 37.0 37.0 35.0 37.0 7 36.38578292500778 37.0 37.0 37.0 35.0 37.0 8 36.386148242275574 37.0 37.0 37.0 35.0 37.0 9 38.21009056791055 39.0 39.0 39.0 37.0 39.0 10-11 38.189707560765555 39.0 39.0 39.0 37.0 39.0 12-13 38.15411023349928 39.0 39.0 39.0 37.0 39.0 14-15 39.69829882873603 41.0 40.0 41.0 37.0 41.0 16-17 39.71217299469028 41.0 40.0 41.0 37.0 41.0 18-19 39.72922415843881 41.0 40.0 41.0 37.0 41.0 20-21 39.72422590020526 41.0 40.0 41.0 37.0 41.0 22-23 39.65600033612345 41.0 40.0 41.0 37.0 41.0 24-25 39.60566525813058 41.0 40.0 41.0 37.0 41.0 26-27 39.50546654289539 41.0 39.5 41.0 36.5 41.0 28-29 39.41892836586011 41.0 39.0 41.0 36.0 41.0 30-31 39.32134387835171 41.0 39.0 41.0 36.0 41.0 32-33 39.34554595008724 41.0 39.0 41.0 36.0 41.0 34-35 39.28374697163426 41.0 39.0 41.0 35.0 41.0 36-37 39.19685393187504 41.0 39.0 41.0 35.0 41.0 38-39 39.107087470603986 41.0 39.0 41.0 35.0 41.0 40-41 38.98920144249034 41.0 39.0 41.0 35.0 41.0 42-43 38.87691017337579 41.0 39.0 41.0 35.0 41.0 44-45 38.73347600169325 41.0 39.0 41.0 35.0 41.0 46-47 38.52166122307116 41.0 39.0 41.0 35.0 41.0 48-49 38.334738910589195 41.0 39.0 41.0 35.0 41.0 50-51 38.20369357061334 41.0 38.0 41.0 34.0 41.0 52-53 38.07696931058793 40.5 38.0 41.0 34.0 41.0 54-55 37.94498819031057 40.0 38.0 41.0 34.0 41.0 56-57 37.794845752082125 40.0 37.0 41.0 34.0 41.0 58-59 37.61295917638709 40.0 37.0 41.0 33.5 41.0 60-61 37.427845302734 40.0 36.5 41.0 33.0 41.0 62-63 37.201633236454555 39.5 36.0 41.0 33.0 41.0 64-65 36.957702690920684 39.0 35.5 41.0 33.0 41.0 66-67 36.67920685912759 39.0 35.0 41.0 32.5 41.0 68-69 36.3651748492869 38.5 35.0 41.0 32.0 41.0 70-71 36.034974394257816 37.0 35.0 40.0 32.0 41.0 72-73 35.687667938438906 37.0 35.0 39.5 31.5 41.0 74-75 35.35223646363416 36.5 35.0 39.0 31.0 41.0 76-77 35.00718404691841 36.0 35.0 39.0 31.0 41.0 78-79 34.665350248916766 35.5 35.0 38.0 31.0 39.5 80-81 34.31993251493323 35.0 35.0 37.0 31.0 39.0 82-83 33.99118287443146 35.0 35.0 37.0 31.0 39.0 84-85 33.71391121054544 35.0 34.0 36.0 30.0 38.0 86-87 33.47400822871228 35.0 34.0 36.0 30.0 37.0 88-89 33.250026925381775 35.0 34.0 36.0 30.0 37.0 90-91 33.04965868873194 35.0 34.0 35.0 30.0 36.5 92-93 32.88429009504955 35.0 34.0 35.0 30.0 36.0 94-95 32.73438317994085 35.0 34.0 35.0 29.5 36.0 96-97 32.61056192975887 35.0 34.0 35.0 29.5 36.0 98-99 32.507583094884254 35.0 34.0 35.0 29.0 36.0 100 32.416935493097434 35.0 34.0 35.0 29.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.09630378123819838 1101 2 0.12130402437922072 1101 3 0.07490996709849895 1101 4 0.05216233315857011 1101 5 0.07609656189340797 1101 6 0.06401429894724942 1101 7 0.06786691361018882 1101 8 0.1085263453037939 1101 9 0.10459763501140884 1101 10-11 0.10058832389480443 1101 12-13 0.11118496062780991 1101 14-15 0.1844721393824429 1101 16-17 0.1745599949405232 1101 18-19 0.16278397400004252 1101 20-21 0.14499830811125491 1101 22-23 0.176202649233538 1101 24-25 0.14352177592304827 1101 26-27 0.14899366641500222 1101 28-29 0.14483307866424155 1101 30-31 0.16804021656940904 1101 32-33 0.1664427297654143 1101 34-35 0.24550339265389454 1101 36-37 0.2919022034572336 1101 38-39 0.23739205087216675 1101 40-41 0.3172704494378422 1101 42-43 0.2661015379135847 1101 44-45 0.22371581547112385 1101 46-47 0.3578355188528448 1101 48-49 0.34919026269552234 1101 50-51 0.33765655584362264 1101 52-53 0.35133395754463237 1101 54-55 0.37057204546197653 1101 56-57 0.3652585930905232 1101 58-59 0.3419802020197764 1101 60-61 0.3007614773791829 1101 62-63 0.32878988970742995 1101 64-65 0.3110430255310064 1101 66-67 0.3275306849699078 1101 68-69 0.41241037848493534 1101 70-71 0.36365103162219725 1101 72-73 0.44893016715072775 1101 74-75 0.3981354068232079 1101 76-77 0.36623432198992845 1101 78-79 0.282066742881959 1101 80-81 0.2991349021297225 1101 82-83 0.32188433884263645 1101 84-85 0.3930610917696242 1101 86-87 0.36881747822421573 1101 88-89 0.3843079272479457 1101 90-91 0.4166375056282092 1101 92-93 0.4232464710280439 1101 94-95 0.3482287049380659 1101 96-97 0.33411163310176306 1101 98-99 0.29480015908927726 1101 100 0.2940114458117833 1102 1 0.1652366211207834 1102 2 0.14360422520793037 1102 3 0.0824897480482889 1102 4 0.06730967880866956 1102 5 0.07233484112536814 1102 6 0.07143036608004394 1102 7 0.07928693687892974 1102 8 0.132158438805682 1102 9 0.12739588911647104 1102 10-11 0.12568403660204552 1102 12-13 0.13759476403942017 1102 14-15 0.16416366943875715 1102 16-17 0.14055076009888268 1102 18-19 0.14935928026623202 1102 20-21 0.21644745427578016 1102 22-23 0.16461569734530457 1102 24-25 0.1493361078983355 1102 26-27 0.2977158547567811 1102 28-29 0.28869617340671994 1102 30-31 0.19556720403264194 1102 32-33 0.15062634353132154 1102 34-35 0.14035953988670968 1102 36-37 0.17067111734585438 1102 38-39 0.1310365792301056 1102 40-41 0.16114099448510188 1102 42-43 0.16712435947501092 1102 44-45 0.11106423351503736 1102 46-47 0.08949149415801827 1102 48-49 0.05823339762946489 1102 50-51 0.07273826487121227 1102 52-53 0.1269435162454755 1102 54-55 0.13160420280983232 1102 56-57 0.09986214043218666 1102 58-59 0.10239077730547308 1102 60-61 0.09682441324611801 1102 62-63 0.06987190703456747 1102 64-65 0.08190870111693016 1102 66-67 0.12157006340713394 1102 68-69 0.06572225997702219 1102 70-71 0.07825951207829007 1102 72-73 0.043184238135495434 1102 74-75 0.08866666418710878 1102 76-77 0.0918588054113556 1102 78-79 0.04832468223857944 1102 80-81 0.018608865605067137 1102 82-83 -0.009214318302213087 1102 84-85 0.0347742069743191 1102 86-87 0.04036323724069035 1102 88-89 0.07838551361280821 1102 90-91 0.02565230207816427 1102 92-93 0.029361314703457708 1102 94-95 0.030840778578898664 1102 96-97 0.019251947978808914 1102 98-99 0.049882400166673335 1102 100 0.11256751084209071 1103 1 0.11626650549591488 1103 2 0.14434463603775072 1103 3 0.12248737910620378 1103 4 0.07184714985277196 1103 5 0.038322747412131264 1103 6 0.06950587230557659 1103 7 0.10142730865070604 1103 8 0.10686749825494957 1103 9 0.15864683025090187 1103 10-11 0.109407727916782 1103 12-13 0.09352122530876272 1103 14-15 0.12418528797574169 1103 16-17 0.1612015392262336 1103 18-19 0.1415161570132497 1103 20-21 0.16730013371072516 1103 22-23 0.1714101833919912 1103 24-25 0.10370692246978308 1103 26-27 0.16155792021347537 1103 28-29 0.1358290471507999 1103 30-31 0.2024735395400441 1103 32-33 0.1929975350222648 1103 34-35 0.16786321360979883 1103 36-37 0.09394122300439989 1103 38-39 0.07731918750054234 1103 40-41 0.1463940971627551 1103 42-43 0.12811909160603108 1103 44-45 0.17713699325805266 1103 46-47 0.1550270116787189 1103 48-49 0.247826732860851 1103 50-51 0.24297089312086229 1103 52-53 0.21809108550288414 1103 54-55 0.1764664704059058 1103 56-57 0.2129786979712378 1103 58-59 0.2532124400544262 1103 60-61 0.2450606923987806 1103 62-63 0.22572598051275605 1103 64-65 0.2205446717172066 1103 66-67 0.23162973870372383 1103 68-69 0.22651206930357404 1103 70-71 0.1678736725770662 1103 72-73 0.2244954739688012 1103 74-75 0.1998002805929815 1103 76-77 0.1465959599548583 1103 78-79 0.10749657863725304 1103 80-81 0.1349268325870412 1103 82-83 0.1616823821619775 1103 84-85 0.2108361636583851 1103 86-87 0.2124871769993959 1103 88-89 0.2516556580810203 1103 90-91 0.21294337023508803 1103 92-93 0.23348758956220905 1103 94-95 0.23667386378910038 1103 96-97 0.19092076677088698 1103 98-99 0.26761266645998205 1103 100 0.2082648538080889 1104 1 0.1040627011895694 1104 2 0.11991101245200753 1104 3 0.1198044534322591 1104 4 0.07045459691700273 1104 5 0.06478080206507997 1104 6 0.03667294625193307 1104 7 0.026016142377656593 1104 8 0.09043836608663014 1104 9 0.11954715282904971 1104 10-11 0.08258730574189599 1104 12-13 0.13364684003468597 1104 14-15 0.15285969051294046 1104 16-17 0.17790706168621995 1104 18-19 0.1976185366088501 1104 20-21 0.17736954481669898 1104 22-23 0.15946190476741862 1104 24-25 0.11689884462516886 1104 26-27 0.13289426952930228 1104 28-29 0.2227829291449268 1104 30-31 0.2152088086931272 1104 32-33 0.17059424474470575 1104 34-35 0.18796447952395567 1104 36-37 0.17727541473158936 1104 38-39 0.19095931907013863 1104 40-41 0.14554387370841937 1104 42-43 0.17224781670016398 1104 44-45 0.19518822666476154 1104 46-47 0.2807242937832015 1104 48-49 0.1749534550276266 1104 50-51 0.17906405920548707 1104 52-53 0.20675900835690442 1104 54-55 0.18543735040297094 1104 56-57 0.20251808482840516 1104 58-59 0.21901519407767012 1104 60-61 0.25735615061304884 1104 62-63 0.20928985588058424 1104 64-65 0.2570571375133426 1104 66-67 0.261280717765203 1104 68-69 0.19959228647296356 1104 70-71 0.20961495998589896 1104 72-73 0.1988229060887079 1104 74-75 0.18016341202280728 1104 76-77 0.13620786542907837 1104 78-79 0.07094723323337604 1104 80-81 0.18056115544925433 1104 82-83 0.2247788421482042 1104 84-85 0.203790880387281 1104 86-87 0.13991754502931286 1104 88-89 0.1606037035482757 1104 90-91 0.15648772083932982 1104 92-93 0.16179581255684639 1104 94-95 0.15809402116765625 1104 96-97 0.20325547300808466 1104 98-99 0.23307387997727602 1104 100 0.18821821181855114 1105 1 -6.377930398002718E-4 1105 2 0.03637428987243396 1105 3 -0.009021678877005002 1105 4 0.02687378684765207 1105 5 0.05516284260927762 1105 6 0.053323469845039995 1105 7 -0.032715300911334566 1105 8 -0.0030122642731882365 1105 9 0.030190979814975094 1105 10-11 0.041955074037325346 1105 12-13 0.014804522284109112 1105 14-15 0.005044226786907302 1105 16-17 0.04840533236006195 1105 18-19 0.0017821713338008749 1105 20-21 -0.016856942457231128 1105 22-23 0.021677738768282495 1105 24-25 0.0112311381192427 1105 26-27 0.022750507106046314 1105 28-29 0.029196002232460216 1105 30-31 0.04978831942079154 1105 32-33 0.04506192773653339 1105 34-35 0.05002381766853148 1105 36-37 0.004264840320303165 1105 38-39 -0.049656634754413176 1105 40-41 0.011822172593412006 1105 42-43 0.04459237016773443 1105 44-45 0.11012814294416984 1105 46-47 0.15884778352958762 1105 48-49 0.09326045151178164 1105 50-51 0.024636808041123004 1105 52-53 0.07192963144873232 1105 54-55 0.07126080458933615 1105 56-57 0.06642605284345393 1105 58-59 -0.010439001527856817 1105 60-61 0.02978672986004227 1105 62-63 0.027228195213290007 1105 64-65 -0.04266366145758127 1105 66-67 0.008524527476645005 1105 68-69 0.016684414363083988 1105 70-71 0.08015555025909293 1105 72-73 0.05327576566591574 1105 74-75 -0.05340289816743393 1105 76-77 -0.02247918561123896 1105 78-79 0.04362007413218549 1105 80-81 0.05121249163644137 1105 82-83 0.04546109475259641 1105 84-85 0.04071132936212507 1105 86-87 0.09500659239923692 1105 88-89 0.027959635643959757 1105 90-91 0.0383341076266035 1105 92-93 0.05011395657232498 1105 94-95 0.0352443406734082 1105 96-97 0.028621949183595063 1105 98-99 -0.0752591018963642 1105 100 -0.08202153376699073 1106 1 0.06444013864984299 1106 2 0.05406688221955136 1106 3 0.0628723760010459 1106 4 0.03175211178579218 1106 5 -0.03948936444268725 1106 6 -0.022778951505259215 1106 7 7.108213933690877E-4 1106 8 0.011318660965415006 1106 9 0.08344025075876971 1106 10-11 0.05442814866616885 1106 12-13 0.05122709320139762 1106 14-15 0.044783865351767815 1106 16-17 0.03309680903026191 1106 18-19 0.06499189650170933 1106 20-21 0.025290928919574185 1106 22-23 0.09902204108359314 1106 24-25 0.10115580231153842 1106 26-27 -0.030223572736652216 1106 28-29 -0.014010519506662433 1106 30-31 0.10106568664419058 1106 32-33 0.090159439003763 1106 34-35 0.0722485713359049 1106 36-37 0.1095884694737137 1106 38-39 0.06907279150044587 1106 40-41 0.0609270075615953 1106 42-43 0.02401075453499857 1106 44-45 0.0927063231757046 1106 46-47 0.0942920659615396 1106 48-49 0.11438292190768351 1106 50-51 0.09852844930128413 1106 52-53 0.08801181044307071 1106 54-55 0.07895814684808755 1106 56-57 0.10801180531782961 1106 58-59 0.08132514879028463 1106 60-61 0.06483417374480638 1106 62-63 0.1345902820814402 1106 64-65 0.0808261704176374 1106 66-67 0.11871294984320002 1106 68-69 0.06666784490390398 1106 70-71 0.03508234086531559 1106 72-73 0.05274554297238865 1106 74-75 0.01322331140308819 1106 76-77 -0.022746644298059948 1106 78-79 0.033457058269696915 1106 80-81 0.039194524819542664 1106 82-83 0.04349706152530075 1106 84-85 0.044675020153917444 1106 86-87 0.028437823620521385 1106 88-89 0.019718677045510447 1106 90-91 -0.04406056584458895 1106 92-93 -0.04110643932728664 1106 94-95 -0.013411600030018178 1106 96-97 0.07346152920302984 1106 98-99 0.06140151691770512 1106 100 0.08240604665011375 1107 1 0.051870751531900794 1107 2 0.07523273132208885 1107 3 0.05753419813719063 1107 4 0.04247205770485607 1107 5 -0.07544809729765234 1107 6 -0.07017491713052237 1107 7 -0.03596579362653074 1107 8 -0.021920083637077425 1107 9 -0.020232834712402337 1107 10-11 0.03604000017111986 1107 12-13 0.0655629414043446 1107 14-15 0.12006736696043419 1107 16-17 0.12039400549965507 1107 18-19 0.10561676420977761 1107 20-21 0.04022553040336874 1107 22-23 0.0939309845473133 1107 24-25 0.11344492897312364 1107 26-27 0.15137981208469853 1107 28-29 0.12997760596958585 1107 30-31 0.14470360230543378 1107 32-33 0.1371325665437766 1107 34-35 0.12886148117630825 1107 36-37 0.1497251468824885 1107 38-39 0.21333274382243417 1107 40-41 0.25724390097663274 1107 42-43 0.2457738024881806 1107 44-45 0.22727853737222148 1107 46-47 0.22639323224721153 1107 48-49 0.1834238980104672 1107 50-51 0.15665821842540595 1107 52-53 0.21885087863162767 1107 54-55 0.13933307541044115 1107 56-57 0.17541368439241722 1107 58-59 0.16151846698942052 1107 60-61 0.1071431838836645 1107 62-63 0.12918556137616122 1107 64-65 0.07458804361846916 1107 66-67 0.12499453454179132 1107 68-69 0.12629684541190755 1107 70-71 0.2102490617716768 1107 72-73 0.13281920311970907 1107 74-75 0.2045770745793618 1107 76-77 0.15797150906532664 1107 78-79 0.17837747805845083 1107 80-81 0.16781148543888236 1107 82-83 0.18930975731736055 1107 84-85 0.1333127969086405 1107 86-87 0.15019577833434283 1107 88-89 0.121570135696345 1107 90-91 0.09771133905338303 1107 92-93 0.13076194607614156 1107 94-95 0.2124828918631323 1107 96-97 0.19961045155296375 1107 98-99 0.20126216721192947 1107 100 0.21077499297134494 1108 1 -0.012533979320920707 1108 2 -0.014832696752954178 1108 3 -0.04830711097876872 1108 4 -0.007310712680521192 1108 5 -0.07835153893807245 1108 6 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16-17 -0.139735993610536 2214 18-19 -0.12244541848621537 2214 20-21 -0.14561899576102633 2214 22-23 -0.12179285190153166 2214 24-25 -0.1611891283576199 2214 26-27 -0.17397177738921954 2214 28-29 -0.0910919053444772 2214 30-31 -0.09558690328141495 2214 32-33 -0.10543213101843918 2214 34-35 -0.09314312478662856 2214 36-37 -0.10593316111559403 2214 38-39 -0.09070372730261766 2214 40-41 -0.09906574410351254 2214 42-43 -0.13651761925754613 2214 44-45 -0.16574614211018712 2214 46-47 -0.09411035477538832 2214 48-49 -0.19438951080635292 2214 50-51 -0.10073725359853825 2214 52-53 -0.15069463260241633 2214 54-55 -0.22115632937299523 2214 56-57 -0.196302079838361 2214 58-59 -0.20718030663154252 2214 60-61 -0.30404722134360895 2214 62-63 -0.24952661390282316 2214 64-65 -0.22652023913093444 2214 66-67 -0.3291088677056777 2214 68-69 -0.20545984263991102 2214 70-71 -0.1480472661787786 2214 72-73 -0.2926038377240303 2214 74-75 -0.19379360624706266 2214 76-77 -0.14440057312683763 2214 78-79 -0.3275021380475849 2214 80-81 -0.2098213509205067 2214 82-83 -0.22818976172000305 2214 84-85 -0.2109440521565915 2214 86-87 -0.157617043414227 2214 88-89 -0.2686622299518433 2214 90-91 -0.17349397853836734 2214 92-93 -0.13423080639125118 2214 94-95 -0.15275752148752275 2214 96-97 -0.30420979562948247 2214 98-99 -0.3800828459104437 2214 100 -0.5033687157554283 2215 1 -0.048571264883904064 2215 2 -0.09412799318847931 2215 3 -0.08101622426085697 2215 4 -0.05404512735866973 2215 5 -0.07029936643539969 2215 6 -0.09753460701274008 2215 7 -0.04007810292151959 2215 8 -0.06302125306328321 2215 9 -0.056465593581926043 2215 10-11 -0.09904691040352276 2215 12-13 -0.10920751380725591 2215 14-15 -0.15463053816299777 2215 16-17 -0.16099942474336615 2215 18-19 -0.13103465876285014 2215 20-21 -0.14414670511405347 2215 22-23 -0.15716966021830814 2215 24-25 -0.13044458294618266 2215 26-27 -0.05333349098800255 2215 28-29 -0.05915541816300873 2215 30-31 -0.08800271439391594 2215 32-33 -0.14380038293315067 2215 34-35 -0.10401837264989666 2215 36-37 -0.13944135834645266 2215 38-39 -0.1355556091580965 2215 40-41 -0.10205471286086265 2215 42-43 -0.11397956539022402 2215 44-45 -0.09962063703647317 2215 46-47 -0.1551630220973621 2215 48-49 -0.1987195770485144 2215 50-51 -0.11654443296737327 2215 52-53 -0.13583760573964554 2215 54-55 -0.1394265818583662 2215 56-57 -0.23621614911547795 2215 58-59 -0.2084385368735724 2215 60-61 -0.16721759644801182 2215 62-63 -0.2105965465642754 2215 64-65 -0.13664134718464993 2215 66-67 -0.20674878414867237 2215 68-69 -0.14234704350420202 2215 70-71 -0.06129593406257072 2215 72-73 -0.009711756048332632 2215 74-75 -0.010263739524951632 2215 76-77 -0.05157191070472322 2215 78-79 -0.08898767361559834 2215 80-81 -0.08338845148615803 2215 82-83 -0.08187633481569634 2215 84-85 -0.07447737726883474 2215 86-87 -0.10724634688923373 2215 88-89 -0.1144403568211132 2215 90-91 -0.06717027652172902 2215 92-93 -0.0516156247823929 2215 94-95 -0.044461557803494145 2215 96-97 0.004400909232259664 2215 98-99 -0.07927190717892785 2215 100 -0.11568403343794387 2216 1 -0.058362165142042954 2216 2 -0.10505045684570291 2216 3 -0.09692011738519568 2216 4 -0.09208359147699241 2216 5 -0.0573451294338696 2216 6 -0.08824411905376195 2216 7 -0.10654226502638409 2216 8 -0.15601213709454953 2216 9 -0.15165950673682005 2216 10-11 -0.18082799661602422 2216 12-13 -0.130915075008474 2216 14-15 -0.2684133348245936 2216 16-17 -0.2093221724512162 2216 18-19 -0.18504943947713315 2216 20-21 -0.1738761511068745 2216 22-23 -0.16009805371358965 2216 24-25 -0.27087351867611176 2216 26-27 -0.3000696651087864 2216 28-29 -0.3025797434925508 2216 30-31 -0.2909709370330589 2216 32-33 -0.2274531326452589 2216 34-35 -0.18231974325845357 2216 36-37 -0.23979493550864817 2216 38-39 -0.18048698032495025 2216 40-41 -0.24534986082464627 2216 42-43 -0.23069396878951665 2216 44-45 -0.13753576787367905 2216 46-47 -0.18710674356226775 2216 48-49 -0.12319010498826088 2216 50-51 -0.12009960956115151 2216 52-53 -0.033607595421123904 2216 54-55 -0.009692371229519381 2216 56-57 0.006048346323112241 2216 58-59 -0.006619901915186688 2216 60-61 -0.04309820059381764 2216 62-63 -0.0030764396998606003 2216 64-65 0.019160468257140906 2216 66-67 0.018271343802638285 2216 68-69 0.016254485115545947 2216 70-71 0.026713871949972656 2216 72-73 0.07820850593535056 2216 74-75 0.10583726091807222 2216 76-77 0.10928805036684253 2216 78-79 0.020290860077459172 2216 80-81 0.01863056428162224 2216 82-83 0.015686214964460987 2216 84-85 0.06118323223505229 2216 86-87 0.05643674888506922 2216 88-89 0.019008387142513072 2216 90-91 -0.03915122942483862 2216 92-93 0.010701320813801374 2216 94-95 -0.0820747944744653 2216 96-97 -0.047499256731711625 2216 98-99 -0.0032941124914671605 2216 100 -0.03906110502536819 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 13.0 3 6.0 4 10.0 5 34.0 6 54.0 7 135.0 8 273.0 9 542.0 10 1032.0 11 1608.0 12 2622.0 13 3995.0 14 4876.0 15 6460.0 16 7952.0 17 11334.0 18 13876.0 19 7540.0 20 4881.0 21 4494.0 22 4584.0 23 4806.0 24 5593.0 25 6640.0 26 8342.0 27 10986.0 28 13686.0 29 17712.0 30 22777.0 31 29022.0 32 37356.0 33 49115.0 34 68636.0 35 106592.0 36 198582.0 37 467514.0 38 911078.0 39 497865.0 40 2160.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.55124230531241 20.207160793056786 13.894723208429536 23.34687369320127 2 15.40196192531428 20.5076624762551 40.6467686226085 23.44360697582212 3 18.02868332318782 26.06534760569248 30.455111936603647 25.450857134516053 4 12.080851198131594 17.219758716732812 39.58994468947996 31.109445395655637 5 12.300234313857434 38.07996823719177 36.1039095328286 13.51588791612219 6 29.953120210385038 38.92671595692093 17.875232613783993 13.244931218910036 7 26.413050982867624 33.090632921380454 22.991547239784193 17.50476885596773 8 24.80965736155258 36.37074118332583 20.58643561883894 18.233165836282648 9 24.97677312302177 16.580262026788134 21.66488676133107 36.778078088859026 10-11 23.580223802945785 26.960268063293203 29.200309534437874 20.259198599323135 12-13 24.719926715620232 24.343247528486657 29.48479218931167 21.452033566581438 14-15 21.68953518648561 26.182883721664208 27.05810760697181 25.069473484878372 16-17 20.758246366651502 29.67877329144152 27.515728170813837 22.047252171093145 18-19 20.527149869298427 28.14827862908921 30.42376030759253 20.90081119401984 20-21 21.72974964720846 27.393035222344476 30.119698609308966 20.7575165211381 22-23 21.303013775370815 27.22363205448999 30.240247911305822 21.23310625883337 24-25 21.21378114839484 27.3712152309775 30.12318813278383 21.291815487843824 26-27 20.92871687856508 27.412388112490234 30.185626204140103 21.473268804804587 28-29 21.022493459414438 27.4373174978369 30.21937938710442 21.320809655644236 30-31 21.25787779370866 27.792149772896167 29.860868563138578 21.0891038702566 32-33 20.93083457604298 27.49656388645652 30.300681145631625 21.27192039186887 34-35 21.078804721006332 27.31788340812838 30.263540060204352 21.339771810660938 36-37 21.403807917913735 27.692099153372908 29.647772427015433 21.25632050169793 38-39 21.28740220555912 27.819553916078892 29.74628098977879 21.146762888583197 40-41 21.172621982705977 27.54338847745054 29.854105954763554 21.42988358507993 42-43 21.210093172573174 27.530489783927152 29.70940961502757 21.5500074284721 44-45 21.31099606761159 27.740532998344058 29.556719933091593 21.391751000952762 46-47 21.442230731427557 27.953864052325233 29.17402464784773 21.429880568399483 48-49 21.482174583615933 27.896972932323116 28.934100980114806 21.686751503946144 50-51 21.452739957618274 27.939862296874868 28.81091287994909 21.796484865557765 52-53 21.501145723028557 27.89951961695607 28.817123824884284 21.78221083513109 54-55 21.577168981658197 28.238004524841354 28.653738379425047 21.5310881140754 56-57 21.463084968573444 27.928376295680845 28.698678492827312 21.9098602429184 58-59 21.290775256554102 27.64284654727018 29.04403120152378 22.022346994651933 60-61 21.57220553866159 27.951280671494466 28.69276981999113 21.783743969852814 62-63 21.35689335341687 27.92897197589029 28.792095933872663 21.92203873682018 64-65 21.369059325522734 27.724262082751988 28.90232189491654 22.00435669680874 66-67 21.28357486464719 27.813057189336526 28.73136735187825 22.172000594138037 68-69 21.70518827030632 27.964039007451042 28.57410001355165 21.75667270869099 70-71 21.639740457406933 27.768706157212875 28.630501343910502 21.96105204146969 72-73 21.47687545603367 27.911183824447527 28.712225026121768 21.899715693397038 74-75 21.542766291944222 27.952038946411207 28.572412038196838 21.932782723447737 76-77 21.529559800036964 27.92468030117938 28.475756267288315 22.070003631495343 78-79 21.6399631921082 27.9990689536479 28.490570689990168 21.87039716425374 80-81 21.537648840169073 27.88267240447442 28.566364705854504 22.013314049502007 82-83 21.43725213456461 27.8983969930181 28.71081864510256 21.953532227314735 84-85 21.399009733224474 27.827380101125488 28.576283897779348 22.197326267870693 86-87 21.5516394538849 27.919615808412342 28.452917159971076 22.07582757773169 88-89 21.326618491602634 27.837530865561273 28.53396128978299 22.3018893530531 90-91 21.256719512762835 27.81686945859644 28.57703344903033 22.349377579610398 92-93 21.396825960412777 27.899257628613107 28.417092985295582 22.286823425678534 94-95 21.18575830751587 27.667200703176565 28.601600215876466 22.5454407734311 96-97 21.131848367296136 27.67755661924512 28.58333435248698 22.607260660971768 98-99 21.13981749128032 27.748351634045203 28.5077065768549 22.604124297819578 100 21.25791438557068 27.609740163161895 28.43418943554537 22.698156015722056 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 31.0 1 27.5 2 114.5 3 226.0 4 159.5 5 120.5 6 312.0 7 705.5 8 1254.5 9 1506.0 10 1236.5 11 999.0 12 1037.5 13 1137.5 14 1300.0 15 1530.0 16 1737.0 17 1967.0 18 2293.5 19 2622.0 20 3213.5 21 4143.0 22 5440.0 23 7645.5 24 10216.0 25 13085.5 26 17326.5 27 23029.5 28 29739.0 29 37158.0 30 44448.0 31 52208.5 32 59829.0 33 66698.5 34 73659.5 35 79676.0 36 84482.5 37 88622.5 38 91375.5 39 92010.0 40 92752.0 41 93561.5 42 92593.5 43 90913.5 44 88567.5 45 85902.0 46 84254.5 47 82028.5 48 79247.5 49 76906.0 50 74548.5 51 71489.0 52 66234.0 53 62256.0 54 60108.0 55 57317.5 56 53100.0 57 48113.0 58 43654.5 59 39491.5 60 35334.5 61 30177.5 62 25619.5 63 21805.0 64 17762.0 65 14197.5 66 11168.0 67 8246.5 68 6048.5 69 4700.0 70 3434.5 71 2452.5 72 1863.5 73 1421.5 74 1084.5 75 778.5 76 502.0 77 338.5 78 228.0 79 131.0 80 72.0 81 34.0 82 18.0 83 8.0 84 3.0 85 3.0 86 2.5 87 1.5 88 1.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09286791019191781 2 0.0011046310473125313 3 0.0 4 1.1835332649777121E-4 5 0.03953001105025559 6 7.89022176651808E-5 7 0.004260719753919763 8 4.7341330599108485E-4 9 1.578044353303616E-4 10-11 3.353344250770184E-4 12-13 0.0 14-15 1.1835332649777121E-4 16-17 0.0 18-19 3.94511088325904E-5 20-21 0.0 22-23 2.169810985792472E-4 24-25 5.9176663248885606E-5 26-27 0.0015188676900547305 28-29 0.00883704837850025 30-31 0.03629502012598317 32-33 0.026432242917835573 34-35 0.02946997829794503 36-37 0.026373066254586686 38-39 0.025288160761690447 40-41 0.031028297096832354 42-43 0.024242706377626804 44-45 0.022585759806658005 46-47 0.01591852241395023 48-49 0.011204114908455675 50-51 0.007890221766518081 52-53 0.005483704127730066 54-55 0.004280445308336059 56-57 0.0025051454108694906 58-59 0.0013807888091406642 60-61 7.495710678192177E-4 62-63 0.001459691026805845 64-65 0.002544596519702081 66-67 7.298455134029225E-4 68-69 0.0014399654723895497 70-71 6.312177413214464E-4 72-73 7.692966222355128E-4 74-75 7.101199589866272E-4 76-77 5.720410780725608E-4 78-79 1.97255544162952E-5 80-81 6.706688501540368E-4 82-83 6.114921869051512E-4 84-85 4.5368775157478964E-4 86-87 2.3670665299554242E-4 88-89 0.0 90-91 3.94511088325904E-5 92-93 1.97255544162952E-5 94-95 0.0 96-97 0.0 98-99 0.0 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2534783.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.432773614194936 #Duplication Level Percentage of deduplicated Percentage of total 1 76.67866727173504 48.639405420861465 2 14.055069752195436 17.831041154454642 3 4.356601031580838 8.290538608909062 4 1.7938365556251585 4.551521125353515 5 0.9343929232109718 2.9635567382373704 6 0.548870902054519 2.0889842204065965 7 0.3527449096030697 1.5662911596087823 8 0.23655996183004685 1.2004523603958364 9 0.16244726285669556 0.9274032404130947 >10 0.8387299017057289 9.095848351202223 >50 0.03338466024697101 1.3957152977211342 >100 0.008321078607700467 0.8805074932784217 >500 1.2459624929821392E-4 0.049594372609973195 >1k 1.868943739441834E-4 0.3055092387585714 >5k 6.229812464806112E-5 0.21363121778926875 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5406 0.21327269434898372 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4295 0.16944251243597577 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 3.9451108832590406E-5 0.0 10-11 0.0 0.0 0.0 7.890221766518081E-5 0.0 12-13 0.0 0.0 0.0 1.380788809140664E-4 0.0 14-15 0.0 0.0 0.0 1.775299897466568E-4 0.0 16-17 0.0 0.0 0.0 1.9725554416295202E-4 0.0 18-19 0.0 0.0 0.0 1.9725554416295202E-4 0.0 20-21 0.0 0.0 0.0 1.9725554416295202E-4 0.0 22-23 0.0 0.0 0.0 2.761577618281328E-4 0.0 24-25 0.0 1.9725554416295203E-5 0.0 3.3533442507701846E-4 0.0 26-27 0.0 3.9451108832590406E-5 0.0 6.509432957377417E-4 0.0 28-29 0.0 3.9451108832590406E-5 0.0 0.0014005143635569593 0.0 30-31 0.0 3.9451108832590406E-5 0.0 0.00489193749524121 0.0 32-33 0.0 5.917666324888561E-5 0.0 0.01311749368683631 0.0 34-35 0.0 7.890221766518081E-5 0.0 0.023532586418640176 0.0 36-37 0.0 9.862777208147601E-5 0.0 0.044086614120419775 0.0 38-39 0.0 1.1835332649777121E-4 0.0 0.07799484216203123 0.0 40-41 0.0 1.5780443533036163E-4 0.0 0.11947768309950005 0.0 42-43 0.0 1.5780443533036163E-4 0.0 0.16273582393443542 0.0 44-45 0.0 1.5780443533036163E-4 0.0 0.20987989898938092 0.0 46-47 0.0 1.5780443533036163E-4 0.0 0.26860287448669173 0.0 48-49 0.0 1.5780443533036163E-4 0.0 0.33679411610382426 0.0 50-51 0.0 1.5780443533036163E-4 0.0 0.4084373297438084 0.0 52-53 0.0 1.5780443533036163E-4 0.0 0.48447934201862647 0.0 54-55 0.0 1.5780443533036163E-4 0.0 0.5645650929487849 0.0 56-57 0.0 1.5780443533036163E-4 0.0 0.6583995553071011 0.0 58-59 0.0 1.5780443533036163E-4 0.0 0.7678763823175396 0.0 60-61 0.0 1.9725554416295202E-4 0.0 0.8856182166284057 0.0 62-63 0.0 1.9725554416295202E-4 0.0 1.0059638241222226 0.0 64-65 0.0 1.9725554416295202E-4 0.0 1.1305504258155432 0.0 66-67 0.0 1.9725554416295202E-4 0.0 1.27373822532343 0.0 68-69 0.0 1.9725554416295202E-4 0.0 1.4412870845354413 0.0 70-71 0.0 1.9725554416295202E-4 0.0 1.6215194752371307 0.0 72-73 0.0 2.3670665299554242E-4 0.0 1.8067818823149753 0.0 74-75 0.0 2.3670665299554242E-4 0.0 1.9934448037563768 0.0 76-77 0.0 2.564322074118376E-4 0.0 2.191390742323899 0.0 78-79 0.0 2.761577618281328E-4 0.0 2.4262037420954776 0.0 80-81 0.0 2.761577618281328E-4 0.0 2.6858314893227546 0.0 82-83 0.0 2.761577618281328E-4 0.0 2.9509626662321784 0.0 84-85 0.0 2.761577618281328E-4 0.0 3.2092688013135637 0.0 86-87 0.0 2.761577618281328E-4 0.0 3.4807516067450353 0.0 88 0.0 2.761577618281328E-4 0.0 3.7092327035489823 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3455 0.0 54.34231 1 GTATCAA 6910 0.0 45.966244 1 ATCAACG 8175 0.0 38.296043 3 TCAACGC 8220 0.0 38.143578 4 CAACGCA 8520 0.0 36.746044 5 TATCAAC 8740 0.0 35.983154 2 AACGCAG 8925 0.0 35.120853 6 ACGCAGA 10210 0.0 30.562565 7 CGCAGAG 10395 0.0 30.017458 8 GCAGAGT 11600 0.0 26.575176 9 GTACATG 6955 0.0 26.048147 1 TACATGG 6990 0.0 25.017868 2 ACATGGG 7025 0.0 24.022354 3 GTGGTAT 1595 0.0 23.896683 1 TGGTATC 1565 0.0 22.828838 2 AGAGTAC 10650 0.0 21.466616 10-11 CAGAGTA 11335 0.0 21.39235 10-11 GAGTACT 8175 0.0 21.268444 12-13 CATGGGG 4970 0.0 20.0515 4 AGTACTT 8480 0.0 19.810797 12-13 >>END_MODULE