FastQCFastQC Report
Wed 25 May 2016
SRR1033118_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033118_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences845885
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA8100.031.9849871
TCAACGC7700.031.1164934
CAACGCA8050.030.3489975
ATCAACG8350.029.821283
AACGCAG8650.027.1511386
GTACATG19200.026.9873331
GGTATCA6050.026.4723241
TACATGG19650.024.4066732
GTCTCGC2351.9645086E-1024.0537011
TCTGTCG2751.8189894E-1223.912758
TATCAAC10850.023.4010282
ACATGGG20000.023.0214673
GAGTACA13650.022.4310321
GTATTGG3400.022.1671351
ACGCAGA10750.021.8471957
CGCAGAG10850.021.645848
GTCCTAA2701.1095835E-920.9356291
GTCTCAC6300.020.9356291
GTACCTG5450.019.8792421
CATGGGG14150.019.588754
Produced by FastQC (version 0.11.5)