##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1033118_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 845885 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.37299751148206 33.0 31.0 34.0 30.0 34.0 2 31.54134072598521 33.0 31.0 34.0 30.0 34.0 3 31.65799133451947 34.0 31.0 34.0 30.0 34.0 4 35.03576490894152 37.0 35.0 37.0 33.0 37.0 5 35.131243608764784 37.0 35.0 37.0 33.0 37.0 6 35.29179025517653 37.0 36.0 37.0 33.0 37.0 7 35.25187229942605 37.0 36.0 37.0 33.0 37.0 8 35.25898437730897 37.0 36.0 37.0 33.0 37.0 9 36.920412349196404 39.0 38.0 39.0 34.0 39.0 10-11 36.901540398517525 39.0 38.0 39.0 34.0 39.0 12-13 36.84887425595678 39.0 38.0 39.0 34.0 39.0 14-15 38.297799937343726 41.0 39.0 41.0 34.0 41.0 16-17 38.30640157941092 41.0 39.0 41.0 34.0 41.0 18-19 38.296504252942185 41.0 39.0 41.0 34.0 41.0 20-21 38.3263049941777 41.0 39.0 41.0 34.0 41.0 22-23 38.28945010255531 41.0 39.0 41.0 34.0 41.0 24-25 38.27572187708731 41.0 39.0 41.0 34.0 41.0 26-27 38.23578914391436 40.5 39.0 41.0 33.5 41.0 28-29 38.20800049652139 40.0 38.5 41.0 33.5 41.0 30-31 38.20788464152929 40.0 39.0 41.0 33.0 41.0 32-33 38.26394249809371 41.0 39.0 41.0 33.5 41.0 34-35 38.24263404599916 41.0 39.0 41.0 33.0 41.0 36-37 38.16035572211353 41.0 39.0 41.0 33.0 41.0 38-39 38.071445291026556 41.0 38.0 41.0 33.0 41.0 40-41 38.00036116020499 41.0 38.0 41.0 33.0 41.0 42-43 37.8889784072303 40.0 38.0 41.0 33.0 41.0 44-45 37.76331475318749 40.0 38.0 41.0 32.5 41.0 46-47 37.63072580788169 40.0 38.0 41.0 32.0 41.0 48-49 37.50756485810719 40.0 38.0 41.0 32.0 41.0 50-51 37.38273997056338 40.0 37.5 41.0 31.5 41.0 52-53 37.21332627957701 40.0 37.0 41.0 31.0 41.0 54-55 37.052254148022485 40.0 37.0 41.0 31.0 41.0 56-57 36.89353044444576 40.0 36.0 41.0 31.0 41.0 58-59 36.69061160796089 40.0 36.0 41.0 30.5 41.0 60-61 36.44726233471453 39.0 35.0 41.0 30.5 41.0 62-63 36.14771866152019 39.0 35.0 41.0 30.0 41.0 64-65 35.84789894607423 38.5 35.0 41.0 30.0 41.0 66-67 35.54356088593603 38.0 35.0 40.0 29.5 41.0 68-69 35.20400763697193 37.0 35.0 40.0 29.0 41.0 70-71 34.830752407242116 37.0 34.5 39.5 29.0 41.0 72-73 34.445661053216455 36.0 34.0 39.0 28.5 41.0 74-75 34.07923299266449 36.0 34.0 39.0 28.0 40.5 76-77 33.758351903627556 35.0 34.0 37.5 28.0 40.0 78-79 33.40396507799524 35.0 34.0 37.0 27.5 39.0 80-81 33.05030352825739 35.0 34.0 37.0 27.0 39.0 82-83 32.85362963050533 35.0 34.0 36.0 27.0 39.0 84-85 32.59340631409707 35.0 34.0 36.0 27.0 37.0 86-87 32.35148985973271 35.0 34.0 36.0 27.0 37.0 88-89 32.00435224646376 35.0 33.0 35.0 26.0 37.0 90-91 31.827818202237893 35.0 33.0 35.0 26.0 36.0 92-93 31.64962908669618 35.0 33.0 35.0 25.0 36.0 94-95 31.473558462438746 35.0 33.0 35.0 25.0 36.0 96-97 31.28919888637344 35.0 33.0 35.0 24.5 36.0 98-99 31.0947469218629 35.0 33.0 35.0 23.5 35.0 100 30.927972478528407 35.0 33.0 35.0 20.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.09154102194342428 1101 2 0.20219963649938322 1101 3 0.179934183365269 1101 4 0.1851786176233361 1101 5 0.06883084497650316 1101 6 0.18775417322277832 1101 7 0.1619334270033761 1101 8 0.15924201221375966 1101 9 0.2884897774873991 1101 10-11 0.2842368141696028 1101 12-13 0.25848205592005513 1101 14-15 0.3783010684946433 1101 16-17 0.08516304178574075 1101 18-19 0.17247395272892874 1101 20-21 0.2430006714029318 1101 22-23 0.27545443840311634 1101 24-25 0.28353507215402374 1101 26-27 0.3524180611785894 1101 28-29 0.4510774941547737 1101 30-31 0.3390363904806577 1101 32-33 0.3030481306595689 1101 34-35 0.3518264048096853 1101 36-37 0.21259568795530726 1101 38-39 0.31725890294140413 1101 40-41 0.31814134071338884 1101 42-43 0.21122023943813417 1101 44-45 0.28079734964872927 1101 46-47 0.27602067802106944 1101 48-49 0.2455488271317705 1101 50-51 0.2227425649057082 1101 52-53 0.2364738437711651 1101 54-55 0.28993187273919574 1101 56-57 0.14499042545956797 1101 58-59 0.2610954611505889 1101 60-61 0.26865898204228955 1101 62-63 0.29868070190001816 1101 64-65 0.23716361697087507 1101 66-67 0.19206086726131844 1101 68-69 0.2930237317866613 1101 70-71 0.31889425838724605 1101 72-73 0.42247834191156386 1101 74-75 0.3951687175816829 1101 76-77 0.41057115574770364 1101 78-79 0.39181878234354883 1101 80-81 0.39583826320406956 1101 82-83 0.32309835918897534 1101 84-85 0.2804136271384934 1101 86-87 0.32782136347503155 1101 88-89 0.36974757694210325 1101 90-91 0.36666946441769355 1101 92-93 0.4074174434012363 1101 94-95 0.3568009243723296 1101 96-97 0.39624971268513676 1101 98-99 0.4058077383312835 1101 100 0.4668777810766258 1102 1 0.17300180550759237 1102 2 0.25025168946403653 1102 3 0.3029389567623433 1102 4 0.19216849236673994 1102 5 0.11977593143572562 1102 6 0.18141200331061924 1102 7 0.23933487561182432 1102 8 0.13738882627054494 1102 9 0.24048677121375306 1102 10-11 0.22487408936625286 1102 12-13 0.2686440975318476 1102 14-15 0.31892608201854955 1102 16-17 0.29494187006029193 1102 18-19 0.2935237105969577 1102 20-21 0.1970323815312156 1102 22-23 0.12230578509542056 1102 24-25 0.13857433114506534 1102 26-27 0.29353390225000453 1102 28-29 0.23284820952961383 1102 30-31 0.3037610005234086 1102 32-33 0.27290533266126005 1102 34-35 0.3363009632812748 1102 36-37 0.3547279495703535 1102 38-39 0.46692596246244733 1102 40-41 0.4116030561646866 1102 42-43 0.45877692082360966 1102 44-45 0.322785284162741 1102 46-47 0.3994758875730611 1102 48-49 0.4360133569975915 1102 50-51 0.4507837121943581 1102 52-53 0.2741741598338052 1102 54-55 0.3606095970304253 1102 56-57 0.29599076157365545 1102 58-59 0.4172915007745388 1102 60-61 0.37131154249594545 1102 62-63 0.4923992633317127 1102 64-65 0.40858685096542047 1102 66-67 0.36091275635232023 1102 68-69 0.29833556055023536 1102 70-71 0.4132395355515399 1102 72-73 0.4756150220564521 1102 74-75 0.39911625493290614 1102 76-77 0.5139536463675256 1102 78-79 0.4333292328951117 1102 80-81 0.319214348903067 1102 82-83 0.3440113633420694 1102 84-85 0.3854312478835098 1102 86-87 0.4134420998389672 1102 88-89 0.4316149287237323 1102 90-91 0.34468969473518385 1102 92-93 0.31025053895428556 1102 94-95 0.29999944662437983 1102 96-97 0.2637475524669064 1102 98-99 0.28795575258944694 1102 100 0.1719946492434481 1103 1 -0.16091164837282435 1103 2 -0.09155730338769885 1103 3 -0.13685141323657746 1103 4 -0.09877179454711893 1103 5 -0.217451092349215 1103 6 -0.011065111425430985 1103 7 0.04856370858626491 1103 8 -0.10104563485646167 1103 9 -0.08122448607462474 1103 10-11 -0.008280291527128725 1103 12-13 0.02943532296275464 1103 14-15 0.08498509699352752 1103 16-17 0.026368180001981045 1103 18-19 -0.01062415131468697 1103 20-21 -0.03722816142229135 1103 22-23 -0.046192194315217705 1103 24-25 -0.06080031857027279 1103 26-27 0.003013956918991312 1103 28-29 -0.01434213883769786 1103 30-31 0.013241055192395379 1103 32-33 6.518101496268969E-4 1103 34-35 -0.1390807855363576 1103 36-37 -0.12070787828132978 1103 38-39 -0.08302339989657526 1103 40-41 -0.04743944995608018 1103 42-43 -0.14592746351116404 1103 44-45 -0.24628101673300762 1103 46-47 -0.17120295179255152 1103 48-49 -0.16963986857096813 1103 50-51 -0.19093285467190668 1103 52-53 -0.12940046431690178 1103 54-55 -0.18376716668352344 1103 56-57 -0.15620828860185298 1103 58-59 -0.19963966153138557 1103 60-61 -0.17959403551060404 1103 62-63 -0.19427418779558536 1103 64-65 -0.28911380683229027 1103 66-67 -0.25843229738257634 1103 68-69 -0.20273618757912004 1103 70-71 -0.15540150748553572 1103 72-73 -0.22070416223506584 1103 74-75 -0.20681474021964874 1103 76-77 -0.12457963190352217 1103 78-79 -0.19430712001483386 1103 80-81 -0.1664940372802377 1103 82-83 -0.213427945232894 1103 84-85 -0.27355619594803926 1103 86-87 -0.236841077648414 1103 88-89 -0.1481860604309695 1103 90-91 0.0446960858937544 1103 92-93 0.008683721849578774 1103 94-95 -0.1263522834130839 1103 96-97 -0.16063520910354967 1103 98-99 -0.0037785128697791492 1103 100 0.20110883466570684 1104 1 0.07590541387527239 1104 2 -0.023972589251258114 1104 3 -0.023821483396300636 1104 4 0.08429657386607659 1104 5 -0.009593576302044937 1104 6 0.06743541104061279 1104 7 0.13900631195990343 1104 8 0.0649492897591486 1104 9 0.17362698429408852 1104 10-11 0.15294246560080182 1104 12-13 0.16234695156403944 1104 14-15 -0.05693907079271554 1104 16-17 0.06806283592077733 1104 18-19 0.06328613380249237 1104 20-21 -0.008842394889967409 1104 22-23 0.1127307792629324 1104 24-25 0.015946210762457724 1104 26-27 0.018524667472803458 1104 28-29 0.17333979130113875 1104 30-31 0.3059433241877656 1104 32-33 0.23829922021082695 1104 34-35 0.15510491382175218 1104 36-37 0.23828226950191578 1104 38-39 0.20225238753438646 1104 40-41 0.28573061273984734 1104 42-43 0.20596274398248937 1104 44-45 0.2416043019712788 1104 46-47 0.10381477473379874 1104 48-49 0.1433219540891315 1104 50-51 0.11480307606402107 1104 52-53 0.10644630385745302 1104 54-55 -0.016820607495581896 1104 56-57 0.023146839029131172 1104 58-59 -0.023605925152821783 1104 60-61 -0.08213545960375512 1104 62-63 0.05526865441967033 1104 64-65 -0.009165307056193228 1104 66-67 -0.06948618602267231 1104 68-69 0.06378680092662847 1104 70-71 -0.10878540806771753 1104 72-73 -0.0801654624291217 1104 74-75 -0.17285911251401842 1104 76-77 -0.16619498494128493 1104 78-79 -0.11260649305558701 1104 80-81 -0.05825959930441371 1104 82-83 -0.07570683014108681 1104 84-85 -0.05912120565097467 1104 86-87 -0.04491877581232728 1104 88-89 0.03982707585802814 1104 90-91 0.08030774435023957 1104 92-93 0.01714114086734142 1104 94-95 0.12465532966349713 1104 96-97 -0.08569398653301619 1104 98-99 -0.061706935022343146 1104 100 -0.17249170272793535 1105 1 -0.07464542417556785 1105 2 -0.07850139378006205 1105 3 -0.02976692684174509 1105 4 0.08977720434671 1105 5 0.03299577878730986 1105 6 -0.013497544892345559 1105 7 -0.12357832562473448 1105 8 0.006729731539593331 1105 9 -0.026256465589362676 1105 10-11 -0.09565268924436054 1105 12-13 -0.08700092278382954 1105 14-15 -0.04542280927645237 1105 16-17 -0.07487061951267293 1105 18-19 -0.1263159307995778 1105 20-21 -0.054959472257046116 1105 22-23 -0.09418730265515762 1105 24-25 -0.07905600923976408 1105 26-27 -0.06161394391580899 1105 28-29 -0.05136258965124796 1105 30-31 -0.03973183398738911 1105 32-33 0.10920609111770574 1105 34-35 -0.10657784786101132 1105 36-37 0.03544344166309088 1105 38-39 -0.15074877796678265 1105 40-41 -0.10701206125282425 1105 42-43 -0.11412688860714582 1105 44-45 -0.12123077961380346 1105 46-47 -0.1951952430262196 1105 48-49 -0.12478147026429554 1105 50-51 -0.019937183676240977 1105 52-53 0.004985264896411934 1105 54-55 -0.1606073762823499 1105 56-57 -0.3340515181692254 1105 58-59 -0.29061669216359576 1105 60-61 -0.19384319631148372 1105 62-63 -0.21471830306728634 1105 64-65 -0.20046012335101437 1105 66-67 -0.21706083359731565 1105 68-69 -0.23239618275635365 1105 70-71 -0.21960167513397977 1105 72-73 -0.21954344555710747 1105 74-75 -0.22346475686966016 1105 76-77 -0.21680062929693378 1105 78-79 -0.2824852254343213 1105 80-81 -0.11278146632627539 1105 82-83 -0.1555193787036373 1105 84-85 -0.19565134218111524 1105 86-87 -0.23265465104820038 1105 88-89 -0.3657118296808761 1105 90-91 -0.18586358432108696 1105 92-93 -0.11619427371807234 1105 94-95 -0.07500611999795126 1105 96-97 -0.01977032685935498 1105 98-99 -0.006269317084722559 1105 100 -0.02827480851103914 1106 1 0.01116627370509704 1106 2 0.10130730327001203 1106 3 0.11072285157906947 1106 4 0.052668718962358696 1106 5 0.12931708200516567 1106 6 0.12548511995238698 1106 7 0.05643801818471417 1106 8 0.17612259920831974 1106 9 0.016887172381856885 1106 10-11 0.1599537732586569 1106 12-13 0.1063684473096771 1106 14-15 0.23448502235552127 1106 16-17 0.1774168439816819 1106 18-19 0.10582979703580975 1106 20-21 0.026046207886565753 1106 22-23 0.08283825799109934 1106 24-25 0.07573125330612385 1106 26-27 0.1138140763397999 1106 28-29 0.18348589519723646 1106 30-31 0.1495113940489361 1106 32-33 0.10333504645355873 1106 34-35 0.09520791426958652 1106 36-37 -0.0075193426790107765 1106 38-39 -0.033445104628626154 1106 40-41 -0.0032724100993775096 1106 42-43 -0.07183058337980697 1106 44-45 -0.11488080774659437 1106 46-47 -0.011123089126478192 1106 48-49 -0.044248019041191355 1106 50-51 -0.11241367270445579 1106 52-53 -0.27988934325405523 1106 54-55 -0.20580403785832146 1106 56-57 -0.03960506424001409 1106 58-59 0.07967441519648588 1106 60-61 0.10421698105903232 1106 62-63 0.08373372383301358 1106 64-65 0.016808718917829424 1106 66-67 0.09497865947665929 1106 68-69 0.09258774136549874 1106 70-71 -0.05760706055315268 1106 72-73 0.04812327469581845 1106 74-75 0.04320391928176548 1106 76-77 -0.025758912393143873 1106 78-79 -0.05038638557686426 1106 80-81 -0.05593649137785661 1106 82-83 -0.010561845815068693 1106 84-85 -0.026261823653662475 1106 86-87 0.06488306028261803 1106 88-89 0.11730132574606955 1106 90-91 0.12243156880164818 1106 92-93 0.2463871104150357 1106 94-95 0.2115899467705269 1106 96-97 0.11875350562999287 1106 98-99 0.042273136630143426 1106 100 0.11691716426713938 1107 1 0.0022362776082474056 1107 2 -0.026080309252478173 1107 3 0.06859212296601314 1107 4 -0.08602576237246495 1107 5 0.024955615696242717 1107 6 0.03586664699338371 1107 7 -9.89264116327604E-4 1107 8 -0.023920728241641598 1107 9 -0.08174782883327225 1107 10-11 -0.030404703087988594 1107 12-13 -0.0846948672279737 1107 14-15 -0.05178545385366107 1107 16-17 0.010605854765678657 1107 18-19 0.05106568001978218 1107 20-21 0.010864723559322442 1107 22-23 -0.09797130252373165 1107 24-25 -0.04320597221509104 1107 26-27 -0.009043178737371704 1107 28-29 0.00303811275259136 1107 30-31 0.06892080285631863 1107 32-33 -0.029322742790924394 1107 34-35 0.056146315646238065 1107 36-37 0.12427343182432793 1107 38-39 0.14189697356698616 1107 40-41 0.17131910901256475 1107 42-43 0.10799889303312682 1107 44-45 0.09365678648754283 1107 46-47 0.07644714921131879 1107 48-49 0.0575426443229361 1107 50-51 0.13988341858759412 1107 52-53 0.17430967580265389 1107 54-55 0.053871914547762856 1107 56-57 0.027814841347883146 1107 58-59 -0.06388397130400847 1107 60-61 0.17187531036238113 1107 62-63 0.05179752687845962 1107 64-65 0.07638549687753482 1107 66-67 0.1887997723415964 1107 68-69 0.10445829661966144 1107 70-71 0.21661611386376478 1107 72-73 0.13931804447118168 1107 74-75 0.026246377727922265 1107 76-77 0.06957313789124697 1107 78-79 0.11689408663097112 1107 80-81 0.1691891950492348 1107 82-83 -0.021422552359354086 1107 84-85 0.07155234945484068 1107 86-87 0.10838480197245559 1107 88-89 0.254616202158509 1107 90-91 0.13810923221241822 1107 92-93 0.1908491135915611 1107 94-95 0.08534597026799773 1107 96-97 0.15639284695456013 1107 98-99 0.09577394549103957 1107 100 0.1912489027902744 1108 1 -0.1284269425588569 1108 2 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0.1570737182652593 2214 58-59 0.09230700139248427 2214 60-61 0.0834726466749629 2214 62-63 0.1823776902760983 2214 64-65 0.07724368006509508 2214 66-67 0.13942512504291926 2214 68-69 0.044546008709808405 2214 70-71 0.16117541203643526 2214 72-73 0.3148241249466679 2214 74-75 0.4412378095007199 2214 76-77 0.3268898706131651 2214 78-79 0.4149350671909744 2214 80-81 0.23602789742401598 2214 82-83 0.21691075467159493 2214 84-85 0.3160717042882837 2214 86-87 0.24412630870704533 2214 88-89 0.14768967885613904 2214 90-91 0.007991900013895048 2214 92-93 0.12526857198382757 2214 94-95 0.1345851149581172 2214 96-97 0.12547104284344357 2214 98-99 0.19776088173352235 2214 100 0.25883912766474637 2215 1 -0.06118441071455294 2215 2 -0.1818214971001666 2215 3 -0.056814953889773534 2215 4 -0.12740501283550998 2215 5 -0.06473831894678739 2215 6 -0.0927726241674236 2215 7 -0.1437593458057549 2215 8 -0.08447335966349812 2215 9 -0.1305845699174668 2215 10-11 -0.2760533696450338 2215 12-13 -0.28065261643553185 2215 14-15 -0.4829477468013934 2215 16-17 -0.3935774382195021 2215 18-19 -0.391703696187335 2215 20-21 -0.3014577187552874 2215 22-23 -0.30369401216186276 2215 24-25 -0.13870656889032773 2215 26-27 -0.2094120917139577 2215 28-29 -0.16101419930285488 2215 30-31 -0.3592681535237148 2215 32-33 -0.2615072795956692 2215 34-35 -0.30273904402221063 2215 36-37 -0.36857596235631007 2215 38-39 -0.3277789549969583 2215 40-41 -0.3229447771855476 2215 42-43 -0.45730923178948757 2215 44-45 -0.4111255695085916 2215 46-47 -0.5411705890015668 2215 48-49 -0.3957552412380636 2215 50-51 -0.3534910172300769 2215 52-53 -0.5681578082466672 2215 54-55 -0.34354905922403844 2215 56-57 -0.2908859750036825 2215 58-59 -0.2634426649895616 2215 60-61 -0.4226384251067188 2215 62-63 -0.31209540044438455 2215 64-65 -0.28444970734059893 2215 66-67 -0.44577206230854927 2215 68-69 -0.4133008636610356 2215 70-71 -0.366990322522625 2215 72-73 -0.30869903471269566 2215 74-75 -0.221417254822164 2215 76-77 -0.44473485723116113 2215 78-79 -0.44188588483498137 2215 80-81 -0.43599278953760034 2215 82-83 -0.33048105366530933 2215 84-85 -0.4789626254923931 2215 86-87 -0.6124295308230785 2215 88-89 -0.33652180049084635 2215 90-91 -0.3007126991702016 2215 92-93 -0.42095707848087827 2215 94-95 -0.3671794121712466 2215 96-97 -0.4952948023838317 2215 98-99 -0.5938814046968091 2215 100 -0.4527902330264659 2216 1 0.019481787832440034 2216 2 -0.16538911974063808 2216 3 -0.00544937537187451 2216 4 -0.1681309654819927 2216 5 -0.06544095739777589 2216 6 -0.11399359042150081 2216 7 -0.12257234117503657 2216 8 -0.12556417029073685 2216 9 -0.3002742800045297 2216 10-11 -0.24636356776980506 2216 12-13 -0.2920802437571979 2216 14-15 -0.33655548087269693 2216 16-17 -0.28657400075248773 2216 18-19 -0.4133780960074347 2216 20-21 -0.36137152966247754 2216 22-23 -0.2480566121536043 2216 24-25 -0.34485408166035114 2216 26-27 -0.4513907234276857 2216 28-29 -0.3893918892699517 2216 30-31 -0.39745501588275545 2216 32-33 -0.3556287061674297 2216 34-35 -0.3513541899883492 2216 36-37 -0.2425833880193693 2216 38-39 -0.17359800379614398 2216 40-41 -0.318776676328703 2216 42-43 -0.30210576036084547 2216 44-45 -0.2680021098288421 2216 46-47 -0.1021406522895063 2216 48-49 -0.14697487656366093 2216 50-51 -0.15949166892608702 2216 52-53 -0.05085459061231745 2216 54-55 -0.02146711992354966 2216 56-57 -0.09604125764240479 2216 58-59 -0.07186858964065124 2216 60-61 0.013506150441834563 2216 62-63 0.0661108751128836 2216 64-65 0.05628794829772232 2216 66-67 0.057519920453636075 2216 68-69 0.09333978105907903 2216 70-71 0.055519120185493875 2216 72-73 0.05748666193657215 2216 74-75 0.0314697126343404 2216 76-77 0.06989181732928529 2216 78-79 0.09094880925798776 2216 80-81 0.11333991045734848 2216 82-83 0.0905145726283294 2216 84-85 0.04005312994720356 2216 86-87 -0.031406046819462574 2216 88-89 0.05581572297251114 2216 90-91 0.06764218143633371 2216 92-93 0.08264331253673163 2216 94-95 0.08511804575535109 2216 96-97 -0.11117186130724832 2216 98-99 -0.08818732549279673 2216 100 -0.04820770857638834 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9627.0 3 1632.0 4 1081.0 5 1017.0 6 813.0 7 823.0 8 946.0 9 1099.0 10 1142.0 11 1299.0 12 1445.0 13 1696.0 14 1947.0 15 2149.0 16 1985.0 17 2056.0 18 1988.0 19 1857.0 20 1910.0 21 1999.0 22 2317.0 23 3077.0 24 4974.0 25 6345.0 26 6577.0 27 5775.0 28 6180.0 29 7790.0 30 9312.0 31 11288.0 32 14748.0 33 19941.0 34 27678.0 35 42605.0 36 79654.0 37 176593.0 38 271623.0 39 110599.0 40 298.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.116197425320294 18.865210037787588 12.199128489658822 23.8194640472333 2 16.911810831635847 19.40613840831269 39.89219732576458 23.789853434286883 3 19.069148464254663 24.1005258145986 28.939203056703988 27.891122664442747 4 12.647915173619243 16.639466594763867 38.24826806498688 32.464350166630005 5 12.571861765224169 36.81876938833678 34.549587426837306 16.059781419601748 6 31.28777560228143 37.51927206000587 17.666330883535274 13.526621454177427 7 27.734479374800486 31.459429422683584 21.832681098591884 18.97341010392405 8 24.97256814215378 35.824232735665674 20.490297162694564 18.712901959485983 9 24.15932081138445 17.43388730246005 21.35955966253407 37.047232223621435 10-11 23.886855347510906 26.520716797222587 28.209479134209985 21.382948721056525 12-13 24.934301891455195 23.649427207086944 28.35397342751688 23.062297473940983 14-15 22.24941217091474 26.165356011287738 25.414791240119833 26.170440577677688 16-17 21.78985842279709 29.460899836464314 25.452797548029 23.296444192709593 18-19 21.41227455605909 27.99563671192543 27.875084620000766 22.71700411201471 20-21 23.23165194028721 27.841666982006696 26.863009195315307 22.063671882390782 22-23 22.505486345620856 28.248176736002723 26.96349688318813 22.282840035188286 24-25 22.353975540592636 27.7082845540569 27.324258614124584 22.613481291225877 26-27 22.12243667247052 27.195851969220854 27.709034796515326 22.9726765617933 28-29 22.364963935201608 27.483741279413504 27.359702021993616 22.791592763391275 30-31 22.766856000250684 28.649125472767356 26.48833402015288 22.095684506829073 32-33 21.662380940273984 27.778779652959635 27.842045607066396 22.71679379969999 34-35 21.697286080409587 27.03969635789094 28.02180358446672 23.241213977232754 36-37 22.933205798876664 28.12069741169293 26.74995817492517 22.19613861450523 38-39 22.571208986107006 28.080461129175287 27.17948566361218 22.168844221105527 40-41 21.76284127937497 27.698844154800025 27.758729137798127 22.779585428026884 42-43 21.661759133420862 27.59147017321297 27.647333882314967 23.099436811051202 44-45 22.395879825890486 27.975164033131172 27.401262370838136 22.227693770140206 46-47 21.991782927406007 28.76785292031213 27.019094348545757 22.221269803736106 48-49 21.750259061844403 27.938127814877628 27.731233016153755 22.580380107124213 50-51 21.919694212814356 27.338951569902235 27.920223878214788 22.821130339068617 52-53 21.97455561591879 27.54622664430534 27.6347221672122 22.84449557256367 54-55 22.239758915783085 28.596889148195505 27.141304174343894 22.022047761677516 56-57 21.555644880032627 28.093112885778293 27.31957605026866 23.031666183920414 58-59 21.36478151744326 27.224814596320655 28.18490595928153 23.225497926954557 60-61 22.328972234422316 28.17948110154348 27.08850687976998 22.403039784264223 62-63 22.012107211658673 28.084451377563298 27.275924665975847 22.627516744802183 64-65 21.837782049554182 27.548169796018733 27.857499213819747 22.75654894060733 66-67 21.737814573685935 27.732137558268615 27.2001981398967 23.32984972814875 68-69 22.69986085019702 27.97230245043158 27.189126690161743 22.13871000920966 70-71 22.76870185734375 27.46836498066757 27.458729799369518 22.304203362619162 72-73 22.08214889701862 27.787954718622853 28.006547132437564 22.12334925192096 74-75 21.964200952757576 27.75871435065238 27.772605530108095 22.504479166481943 76-77 22.13771774058162 28.050318852367923 27.153879074914343 22.65808433213611 78-79 22.513507123012356 28.210202754625524 27.21599574392623 22.060294378435895 80-81 22.03647820676895 27.614966054755737 27.69742772781146 22.65112801066386 82-83 21.79414275209314 27.840606815801234 27.78338680960634 22.581863622499284 84-85 22.024623722144916 27.85987130121026 27.482795433238245 22.63270954340658 86-87 22.467331948544494 27.977305856663715 27.340555200228877 22.21480699456291 88-89 21.871952524796043 27.777513498085295 27.60138429702923 22.74914968008943 90-91 21.6230938762597 27.577704908673763 27.59970836500047 23.19949285006607 92-93 22.198720186293112 28.18902376295676 27.103067164352172 22.50918888639795 94-95 21.93434187579861 27.72177737028587 27.482752449262204 22.861128304653317 96-97 21.81134431297937 27.681074974730958 27.759319816873774 22.748260895415896 98-99 21.778738047985236 27.819462594846172 27.676056254288216 22.725743102880376 100 22.242648588935754 27.87929523485631 27.31648673362974 22.561569442578193 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 279.0 1 243.0 2 189.0 3 149.5 4 121.5 5 99.0 6 85.5 7 84.0 8 89.5 9 94.0 10 90.0 11 87.0 12 78.0 13 83.0 14 100.0 15 121.0 16 159.5 17 211.5 18 268.0 19 371.0 20 562.0 21 833.5 22 1219.5 23 1788.0 24 2518.0 25 3459.5 26 4719.5 27 6206.5 28 8080.5 29 10098.0 30 12163.5 31 14427.5 32 16849.0 33 19103.0 34 21063.0 35 22799.5 36 24370.5 37 26061.0 38 27219.0 39 27829.5 40 28328.0 41 28557.5 42 28314.5 43 28067.5 44 27875.5 45 27757.5 46 27934.5 47 27886.5 48 28479.0 49 29223.5 50 28740.0 51 27843.0 52 26371.5 53 24696.0 54 23756.5 55 23103.5 56 22038.0 57 20719.5 58 19143.0 59 17246.0 60 15164.5 61 12837.5 62 10582.5 63 8607.5 64 6896.0 65 5371.0 66 4116.5 67 3041.5 68 2282.5 69 1739.0 70 1250.0 71 910.0 72 711.0 73 564.5 74 448.0 75 316.0 76 210.5 77 153.5 78 107.5 79 79.0 80 56.5 81 32.5 82 20.5 83 16.5 84 12.0 85 9.5 86 5.5 87 5.5 88 6.5 89 3.0 90 2.0 91 2.5 92 1.5 93 1.0 94 1.0 95 0.5 96 0.0 97 1.0 98 1.0 99 0.5 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.32522151356271833 2 0.11916513474053803 3 0.06265627124254479 4 0.03534759453117149 5 0.03995815033958517 6 0.011940157349994384 7 0.008866453477718602 8 0.01808756509454595 9 0.0200972945494955 10-11 0.024412301908651887 12-13 0.01897421044231781 14-15 0.02228435307399942 16-17 0.023407437181177108 18-19 0.02275723059281108 20-21 0.028017992989590786 22-23 0.01761468757573429 24-25 0.028254431748996615 26-27 0.021575036795781932 28-29 0.02187058524503922 30-31 0.02358476625073148 32-33 0.0293775158561743 34-35 0.018028455404694492 36-37 0.011881047660142927 38-39 0.015959616259893485 40-41 0.011467279831182727 42-43 0.009043782547272975 44-45 0.011289950761628354 46-47 0.01004864727474775 48-49 0.005260762396779704 50-51 0.0080389178197982 52-53 0.009398440686381718 54-55 0.0046696654982651305 56-57 0.0035465813910874407 58-59 0.003310142631681612 60-61 0.004196787979453472 62-63 0.003014594182424325 64-65 0.002719045733167038 66-67 0.0021279488346524645 68-69 0.0040194589098991 70-71 0.003132813562127239 72-73 0.0021870585245039216 74-75 0.00289637480272141 76-77 0.004610555808413673 78-79 0.004137678289602015 80-81 0.004433226738859301 82-83 0.003191923251978697 84-85 0.0040194589098991 86-87 0.003310142631681612 88-89 0.5940523830071464 90-91 0.6038645915224883 92-93 0.5981309516068969 94-95 0.5873729880539317 96-97 0.5834126388338841 98-99 0.5814029093789346 100 0.582112225657152 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 845885.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 83.34383796183265 #Duplication Level Percentage of deduplicated Percentage of total 1 88.35819292126486 73.64110913430247 2 8.482594881350083 14.139440265742248 3 1.7389053317638472 4.347811326044787 4 0.5772568925524301 1.924432196609631 5 0.2785682453565454 1.1608473351153983 6 0.15352204094558589 0.7677069662483249 7 0.1121546777756541 0.6543180903836968 8 0.06470631989028268 0.43142984320337824 9 0.04843543261898473 0.36331153630271346 >10 0.18339125651982513 2.3984959828712396 >50 0.001988145230575189 0.09858695470524065 >100 2.8385473131163614E-4 0.0725103684708814 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 2.364387594058294E-4 0.0 5 0.0 0.0 0.0 2.364387594058294E-4 0.0 6 0.0 0.0 0.0 2.364387594058294E-4 0.0 7 0.0 0.0 0.0 2.364387594058294E-4 0.0 8 0.0 0.0 0.0 2.364387594058294E-4 0.0 9 0.0 0.0 0.0 3.546581391087441E-4 0.0 10-11 0.0 0.0 0.0 3.546581391087441E-4 0.0 12-13 0.0 0.0 0.0 3.546581391087441E-4 0.0 14-15 0.0 0.0 0.0 4.137678289602015E-4 0.0 16-17 0.0 0.0 0.0 4.728775188116588E-4 0.0 18-19 0.0 0.0 0.0 4.728775188116588E-4 0.0 20-21 0.0 0.0 0.0 4.728775188116588E-4 0.0 22-23 0.0 0.0 0.0 5.910968985145734E-4 0.0 24-25 0.0 0.0 0.0 7.093162782174882E-4 0.0 26-27 0.0 0.0 0.0 7.093162782174882E-4 0.0 28-29 0.0 0.0 0.0 9.457550376233176E-4 0.0 30-31 0.0 0.0 0.0 0.002837265112869953 0.0 32-33 0.0 0.0 0.0 0.010580634483410865 0.0 34-35 0.0 0.0 0.0 0.020451952688604245 0.0 36-37 0.0 0.0 0.0 0.03641156894849773 0.0 38-39 0.0 0.0 0.0 0.06301092938165354 0.0 40-41 0.0 0.0 0.0 0.10669299018188051 0.0 42-43 0.0 0.0 0.0 0.15463094865141241 0.0 44-45 0.0 0.0 0.0 0.2001454098370346 0.0 46-47 0.0 0.0 0.0 0.2523983756657229 0.0 48-49 0.0 0.0 0.0 0.31735992481247455 0.0 50-51 0.0 0.0 0.0 0.38947374643125243 0.0 52-53 0.0 0.0 0.0 0.46489771068171204 0.0 54-55 0.0 0.0 0.0 0.5444002435319222 0.0 56-57 0.0 0.0 0.0 0.6338332042771773 0.0 58-59 0.0 0.0 0.0 0.7320735088102992 0.0 60-61 0.0 0.0 0.0 0.837111427676339 0.0 62-63 0.0 0.0 0.0 0.9468781217304953 0.0 64-65 0.0 0.0 0.0 1.0726635417343966 0.0 66-67 0.0 0.0 0.0 1.221087972951406 0.0 68-69 0.0 0.0 0.0 1.3655520549483677 0.0 70-71 0.0 0.0 0.0 1.527926372970321 0.0 72-73 0.0 0.0 0.0 1.724111433587308 0.0 74-75 0.0 0.0 0.0 1.9120211376250908 0.0 76-77 0.0 0.0 0.0 2.096620699031192 0.0 78-79 0.0 0.0 0.0 2.3079969499400037 0.0 80-81 0.0 0.0 0.0 2.5457952322124164 0.0 82-83 0.0 0.0 0.0 2.80268594430685 0.0 84-85 0.0 0.0 0.0 3.0549661005928703 0.0 86-87 0.0 0.0 0.0 3.3204868274056167 0.0 88 0.0 0.0 0.0 3.5347594531171493 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 810 0.0 31.984987 1 TCAACGC 770 0.0 31.116493 4 CAACGCA 805 0.0 30.348997 5 ATCAACG 835 0.0 29.82128 3 AACGCAG 865 0.0 27.151138 6 GTACATG 1920 0.0 26.987333 1 GGTATCA 605 0.0 26.472324 1 TACATGG 1965 0.0 24.406673 2 GTCTCGC 235 1.9645086E-10 24.053701 1 TCTGTCG 275 1.8189894E-12 23.91275 8 TATCAAC 1085 0.0 23.401028 2 ACATGGG 2000 0.0 23.021467 3 GAGTACA 1365 0.0 22.431032 1 GTATTGG 340 0.0 22.167135 1 ACGCAGA 1075 0.0 21.847195 7 CGCAGAG 1085 0.0 21.64584 8 GTCCTAA 270 1.1095835E-9 20.935629 1 GTCTCAC 630 0.0 20.935629 1 GTACCTG 545 0.0 19.879242 1 CATGGGG 1415 0.0 19.58875 4 >>END_MODULE