FastQCFastQC Report
Wed 25 May 2016
SRR1033118_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033118_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences845885
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15850.1873777168291198No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14920.1763833145167487No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16350.042.574441
GTCTCGC3250.036.1794241
TCTGTCG3900.032.5326088
GTATCAA26050.031.9573351
TATAACG753.2714583E-431.3388162
TTACGCT600.004158010231.3369584
CTGTCGC4200.030.2088539
TCTCGCT3750.030.085262
ATAACGC1005.3176947E-528.2032643
TCGCTCT4250.027.6502594
ATCAACG29450.027.293483
TCAACGC29450.027.293484
TATCAAC30600.026.8837852
CAACGCA30150.026.6598015
AACGCAG30950.026.1148516
GTACATG22900.025.2624031
CGCTCTG3550.025.157845
TACATGG22500.024.8621272
CTCGCTC4350.024.853453
ACATGGG22100.024.247153
Produced by FastQC (version 0.11.5)