FastQCFastQC Report
Wed 25 May 2016
SRR1033117_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033117_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2858876
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA31350.031.5526071
TCAACGC30400.031.0580164
ATCAACG31600.029.8843483
GGTATCA23700.029.4148641
CAACGCA32400.028.9953675
GTACATG75300.028.8376241
AACGCAG33900.027.844136
TACATGG75900.026.9360772
ACATGGG74700.025.9755233
TATCAAC38400.025.2129042
GCGTTAG1501.1414431E-625.1218851
ACGCAGA39900.023.0685587
CGCAGAG41900.021.6311978
CATGGGG53500.019.5795334
GAGTACA52500.017.9442021
ACGCGTT1052.1599692E-517.889910-11
GACGCGT1103.2210024E-517.07672510-11
GTATATA23100.016.7207341
GCAGAGT53750.016.6001649
CGCGACG850.001931483516.57330750-51
Produced by FastQC (version 0.11.5)