##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033117_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2858876
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.801747260112016	33.0	31.0	34.0	30.0	34.0
2	31.844045352089424	34.0	31.0	34.0	30.0	34.0
3	32.01382536353448	34.0	31.0	34.0	30.0	34.0
4	35.383309034739526	37.0	35.0	37.0	33.0	37.0
5	35.4444876238074	37.0	35.0	37.0	35.0	37.0
6	35.53043818619626	37.0	37.0	37.0	35.0	37.0
7	35.43631623057453	37.0	36.0	37.0	35.0	37.0
8	35.439055418982846	37.0	37.0	37.0	35.0	37.0
9	37.12997205894904	39.0	38.0	39.0	35.0	39.0
10-11	37.139590349494	39.0	38.0	39.0	35.0	39.0
12-13	37.112416907903665	39.0	38.0	39.0	35.0	39.0
14-15	38.590161657938296	41.0	39.0	41.0	35.0	41.0
16-17	38.60143077209365	41.0	39.0	41.0	35.0	41.0
18-19	38.58986713659494	41.0	39.0	41.0	35.0	41.0
20-21	38.62417187733921	41.0	39.0	41.0	35.0	41.0
22-23	38.58592922533191	41.0	39.0	41.0	35.0	41.0
24-25	38.583717342060304	41.0	39.0	41.0	34.0	41.0
26-27	38.54916495154039	41.0	39.0	41.0	34.0	41.0
28-29	38.525762922211385	40.5	39.0	41.0	34.0	41.0
30-31	38.526527033701356	41.0	39.0	41.0	34.0	41.0
32-33	38.59523183237049	41.0	39.0	41.0	35.0	41.0
34-35	38.56744538762787	41.0	39.0	41.0	34.0	41.0
36-37	38.49402877214682	41.0	39.0	41.0	34.0	41.0
38-39	38.421045893560965	41.0	39.0	41.0	34.0	41.0
40-41	38.364213767928376	41.0	39.0	41.0	34.0	41.0
42-43	38.254916792473686	41.0	38.5	41.0	33.5	41.0
44-45	38.13514157312174	40.0	38.0	41.0	33.0	41.0
46-47	38.01812250688732	40.0	38.0	41.0	33.0	41.0
48-49	37.9062411241341	40.0	38.0	41.0	33.0	41.0
50-51	37.79052221922182	40.0	38.0	41.0	33.0	41.0
52-53	37.657605121733155	40.0	37.5	41.0	33.0	41.0
54-55	37.512837387840534	40.0	37.0	41.0	32.0	41.0
56-57	37.36820502183375	40.0	37.0	41.0	32.0	41.0
58-59	37.18580116801148	40.0	36.5	41.0	32.0	41.0
60-61	36.973402484053175	40.0	36.0	41.0	31.0	41.0
62-63	36.72313891893178	39.0	35.5	41.0	31.0	41.0
64-65	36.455476557919965	39.0	35.0	41.0	31.0	41.0
66-67	36.16526040303952	38.5	35.0	41.0	31.0	41.0
68-69	35.85554987344677	38.0	35.0	40.0	30.0	41.0
70-71	35.504320054454965	37.0	35.0	40.0	30.0	41.0
72-73	35.13280691432577	37.0	35.0	39.5	30.0	41.0
74-75	34.75715805792206	36.0	34.5	39.0	29.0	41.0
76-77	34.417502193169625	36.0	34.0	39.0	29.0	40.0
78-79	34.04096172761603	35.0	34.0	37.5	29.0	39.5
80-81	33.671581943393136	35.0	34.0	37.0	29.0	39.0
82-83	33.44075661203914	35.0	34.0	37.0	29.0	39.0
84-85	33.162602540299055	35.0	34.0	36.0	29.0	37.5
86-87	32.90236669936017	35.0	34.0	36.0	29.0	37.0
88-89	32.5374313191618	35.0	34.0	35.5	28.0	37.0
90-91	32.33760103621144	35.0	34.0	35.0	27.0	36.0
92-93	32.15652427737334	35.0	34.0	35.0	27.0	36.0
94-95	31.985365927028667	35.0	34.0	35.0	27.0	36.0
96-97	31.813478793763704	35.0	34.0	35.0	26.5	36.0
98-99	31.62677709701295	35.0	33.0	35.0	26.0	35.5
100	31.471445071419677	35.0	33.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.15764301910842704
1101	2	0.19208752758138203
1101	3	0.219980738695142
1101	4	0.1159082137479146
1101	5	0.15856433267749992
1101	6	0.12134217546884685
1101	7	0.13926102037341082
1101	8	0.11305495855165049
1101	9	0.2355378420806531
1101	10-11	0.19818874602589176
1101	12-13	0.18078100712337886
1101	14-15	0.23757908504323666
1101	16-17	0.22024937438853698
1101	18-19	0.19397782550937137
1101	20-21	0.25296340598495703
1101	22-23	0.2534244252800377
1101	24-25	0.1903554088748649
1101	26-27	0.1864115151089436
1101	28-29	0.30481255921532835
1101	30-31	0.31227794895569616
1101	32-33	0.22108900250195518
1101	34-35	0.2326124026914016
1101	36-37	0.2245939834191617
1101	38-39	0.208362479128418
1101	40-41	0.24699868218450405
1101	42-43	0.22392328989452182
1101	44-45	0.19473123208336318
1101	46-47	0.2296855339494499
1101	48-49	0.2196113915750857
1101	50-51	0.22088855674898866
1101	52-53	0.18621938986562014
1101	54-55	0.14220295095933722
1101	56-57	0.23992535488698508
1101	58-59	0.2501352002553432
1101	60-61	0.2721570119697887
1101	62-63	0.27393117906981246
1101	64-65	0.2639011280779755
1101	66-67	0.23697476640855086
1101	68-69	0.22700200839966556
1101	70-71	0.2770597966813071
1101	72-73	0.29898444299739424
1101	74-75	0.2616666471615332
1101	76-77	0.26493545536074237
1101	78-79	0.2651500547810599
1101	80-81	0.2674851602653021
1101	82-83	0.2515738523869828
1101	84-85	0.20148551233042866
1101	86-87	0.26967013430731157
1101	88-89	0.3461848577481774
1101	90-91	0.3354788654511083
1101	92-93	0.35647079494024325
1101	94-95	0.3351907937098204
1101	96-97	0.30423290614179166
1101	98-99	0.40197888928112135
1101	100	0.40391439991266154
1102	1	0.3138551384448327
1102	2	0.25179699610954387
1102	3	0.3141284050657305
1102	4	0.35056154796846073
1102	5	0.25507446290187374
1102	6	0.2722875607722557
1102	7	0.20224724689965257
1102	8	0.2407972606849853
1102	9	0.34527016151465517
1102	10-11	0.3579743820909087
1102	12-13	0.37098484829359535
1102	14-15	0.38106178618537
1102	16-17	0.421921320491343
1102	18-19	0.3746099161248182
1102	20-21	0.37758073068549436
1102	22-23	0.3522078466641503
1102	24-25	0.36840574689288985
1102	26-27	0.3727037828369646
1102	28-29	0.3898976233887197
1102	30-31	0.38800281399092285
1102	32-33	0.39423119935606366
1102	34-35	0.44300318799915317
1102	36-37	0.4090891602274027
1102	38-39	0.31562771959937663
1102	40-41	0.37382914032711057
1102	42-43	0.41147961118753784
1102	44-45	0.3385004399977234
1102	46-47	0.38183194584141233
1102	48-49	0.3517144512236854
1102	50-51	0.3332477309492319
1102	52-53	0.29356371226662503
1102	54-55	0.3588761234284803
1102	56-57	0.33792005249677715
1102	58-59	0.3217364246099663
1102	60-61	0.3266859209447759
1102	62-63	0.40168173435198895
1102	64-65	0.40727673171844003
1102	66-67	0.38873324806644405
1102	68-69	0.3515486977521789
1102	70-71	0.34955848649392607
1102	72-73	0.33841605379541306
1102	74-75	0.331911614804028
1102	76-77	0.3293749775756396
1102	78-79	0.39521445771216435
1102	80-81	0.3569976841607456
1102	82-83	0.4010605581116806
1102	84-85	0.3982835871802948
1102	86-87	0.34946360828462275
1102	88-89	0.5195499218596709
1102	90-91	0.5492951266302555
1102	92-93	0.5945197840842482
1102	94-95	0.5566411853217303
1102	96-97	0.5418957704280416
1102	98-99	0.6227318898165457
1102	100	0.6235902678086376
1103	1	0.12119728656879758
1103	2	0.06833223935869981
1103	3	0.1432982212734828
1103	4	0.17249200671772513
1103	5	0.14265805197393888
1103	6	0.17167407752909014
1103	7	0.1695315102442052
1103	8	0.11286011265256235
1103	9	0.13113383148043312
1103	10-11	0.2282128168620119
1103	12-13	0.20433557434666483
1103	14-15	0.20400067338704275
1103	16-17	0.21524415573109223
1103	18-19	0.2521894449779225
1103	20-21	0.19425648204328638
1103	22-23	0.1648423949667972
1103	24-25	0.1320789672555236
1103	26-27	0.18443826559792598
1103	28-29	0.20787530149206646
1103	30-31	0.23479704398029355
1103	32-33	0.1983422773802559
1103	34-35	0.21903911862543168
1103	36-37	0.2558751612926571
1103	38-39	0.20883770454506845
1103	40-41	0.2365910273778269
1103	42-43	0.19481804933034397
1103	44-45	0.18469676314183658
1103	46-47	0.1990994708762983
1103	48-49	0.23272753718549666
1103	50-51	0.20426218100160298
1103	52-53	0.21532877272333195
1103	54-55	0.24691769681602693
1103	56-57	0.16908653854831357
1103	58-59	0.2508789158584932
1103	60-61	0.25925269249992056
1103	62-63	0.23077132625467556
1103	64-65	0.2632063927243635
1103	66-67	0.2131370686788614
1103	68-69	0.15131481138788416
1103	70-71	0.17576225327007222
1103	72-73	0.20166988873601355
1103	74-75	0.23351464004007028
1103	76-77	0.21683353014918794
1103	78-79	0.16758626725503944
1103	80-81	0.25179918001272483
1103	82-83	0.2320095786632166
1103	84-85	0.1516492941900438
1103	86-87	0.26698494785730986
1103	88-89	0.30003711484913964
1103	90-91	0.3461222199221581
1103	92-93	0.26575235301936573
1103	94-95	0.2538610972469755
1103	96-97	0.26815077843721724
1103	98-99	0.20955224771865488
1103	100	0.22128137347584698
1104	1	0.12510200045629816
1104	2	0.1282213526040934
1104	3	0.08032358514910243
1104	4	0.11384057973583595
1104	5	0.08149068615941246
1104	6	0.11642194138194384
1104	7	0.06958275625070343
1104	8	0.09200116666453084
1104	9	0.12490773145054845
1104	10-11	0.05367649387474671
1104	12-13	0.10399295464087999
1104	14-15	0.13701825184351435
1104	16-17	0.1517502295005002
1104	18-19	0.08452683411393735
1104	20-21	0.08701489975867105
1104	22-23	0.0800337727227145
1104	24-25	0.11117738525238963
1104	26-27	0.10940467421321642
1104	28-29	0.15097257204200787
1104	30-31	0.0569284887451289
1104	32-33	0.08032454576527925
1104	34-35	0.06905720788621039
1104	36-37	0.09970254255549804
1104	38-39	0.025801084761461368
1104	40-41	0.07589278191193216
1104	42-43	0.04605499049844042
1104	44-45	0.019105931458064163
1104	46-47	-0.023741594477677097
1104	48-49	-0.06050124270421264
1104	50-51	-0.11821511221579328
1104	52-53	-0.14079413159234377
1104	54-55	-0.12618696673614238
1104	56-57	-0.11874568086738435
1104	58-59	-0.10957299278618393
1104	60-61	-0.14250047560436485
1104	62-63	-0.19374492055072778
1104	64-65	-0.18351783670210153
1104	66-67	-0.1958805726967796
1104	68-69	-0.19240535892436839
1104	70-71	-0.20218035616995422
1104	72-73	-0.09636454957381346
1104	74-75	-0.11238638314149796
1104	76-77	-0.17181465152825126
1104	78-79	-0.18919832317843088
1104	80-81	-0.14296023281897874
1104	82-83	-0.01830750082770294
1104	84-85	-0.01637096247049641
1104	86-87	-0.05661019544523782
1104	88-89	0.08088302994634944
1104	90-91	0.0495574823074989
1104	92-93	-0.008364729617504452
1104	94-95	0.05610863615544304
1104	96-97	0.027579132960234176
1104	98-99	-0.01937252165361869
1104	100	-0.023574777576516937
1105	1	-0.028130351237592777
1105	2	-0.01960848137958493
1105	3	-0.04989787430312731
1105	4	-0.10651410831115982
1105	5	-0.18872047020558824
1105	6	-0.10575531156453621
1105	7	-0.12094181888925704
1105	8	-0.10579539574742114
1105	9	-0.09441613556563055
1105	10-11	-0.11841981437540738
1105	12-13	-0.07334825116186039
1105	14-15	-0.02978446111304578
1105	16-17	-0.08796859867986484
1105	18-19	-0.05264129690035446
1105	20-21	-0.04995018643718652
1105	22-23	-0.02246552285350134
1105	24-25	-0.03677489039219495
1105	26-27	0.021119796697568916
1105	28-29	0.02640434824301252
1105	30-31	-0.011447152707440011
1105	32-33	-0.05443072745153188
1105	34-35	-0.03041329312274854
1105	36-37	-0.03439933846946275
1105	38-39	-0.09016605351336437
1105	40-41	-0.020131677958673322
1105	42-43	-0.016423186595126538
1105	44-45	-0.07216445058154619
1105	46-47	-0.024291016565562984
1105	48-49	-0.07711901239890295
1105	50-51	-0.019074765383138015
1105	52-53	-0.053773061494354124
1105	54-55	-0.12604889967499133
1105	56-57	-0.010222665421288468
1105	58-59	-0.08799468505402785
1105	60-61	-0.13779358705131983
1105	62-63	-0.07609359751479161
1105	64-65	-0.10588193983544159
1105	66-67	-0.0966174426644244
1105	68-69	-0.15319075586361208
1105	70-71	-0.14766108626607632
1105	72-73	-0.10535760779764303
1105	74-75	-0.12661545890904335
1105	76-77	-0.08297104499233399
1105	78-79	-0.09915443544223734
1105	80-81	-0.11036929099726933
1105	82-83	-0.07049203993531705
1105	84-85	-0.11185616103261253
1105	86-87	-0.013582994725730657
1105	88-89	-0.01415976201952418
1105	90-91	0.04935947518642081
1105	92-93	0.04662742922080554
1105	94-95	0.10673385139604363
1105	96-97	0.1496556604213808
1105	98-99	0.18858903246178116
1105	100	0.24612128135031242
1106	1	0.12662609693176208
1106	2	0.07889307122393774
1106	3	0.11354547964936756
1106	4	0.08128415463656324
1106	5	0.12908132823824303
1106	6	0.09772262995451797
1106	7	0.06367685455971639
1106	8	0.12289068782732215
1106	9	0.1085757711630535
1106	10-11	0.06574639779272928
1106	12-13	0.09175451118428413
1106	14-15	0.13651486111317013
1106	16-17	0.14118554072830847
1106	18-19	0.14072803088946984
1106	20-21	0.10129770665379567
1106	22-23	0.0807561089012836
1106	24-25	0.14497641349328916
1106	26-27	0.16173135530321758
1106	28-29	0.15047441684259866
1106	30-31	0.21081249522292467
1106	32-33	0.1785766916089031
1106	34-35	0.13225998044516984
1106	36-37	0.14891731316905776
1106	38-39	0.15518386467689282
1106	40-41	0.16442694687037118
1106	42-43	0.158222564917061
1106	44-45	0.13827290947173054
1106	46-47	0.1757360889045927
1106	48-49	0.16436926702039756
1106	50-51	0.12297198538631449
1106	52-53	0.11169023923384458
1106	54-55	0.11381612111317452
1106	56-57	0.14245027926327225
1106	58-59	0.07604339963891249
1106	60-61	0.10257172013943716
1106	62-63	0.15501312239444331
1106	64-65	0.16839801116094577
1106	66-67	0.21030829273247065
1106	68-69	0.1540256576085497
1106	70-71	0.163055852049105
1106	72-73	0.1932982733219859
1106	74-75	0.19128566230914146
1106	76-77	0.13821098920604413
1106	78-79	0.12440619283460563
1106	80-81	0.16727867225549176
1106	82-83	0.14174362691680642
1106	84-85	0.18972225983393542
1106	86-87	0.14799630316311152
1106	88-89	0.23555515092231616
1106	90-91	0.18879291878703697
1106	92-93	0.17184826069274806
1106	94-95	0.18487072716857256
1106	96-97	0.23326353827410173
1106	98-99	0.239274069091163
1106	100	0.2720932550067552
1107	1	0.056620588160569696
1107	2	0.030426794256140255
1107	3	0.045275006080402136
1107	4	-0.056136278784357785
1107	5	0.009553120967787265
1107	6	0.017076698199517182
1107	7	-0.04365881411412431
1107	8	0.011109765969784746
1107	9	-0.05799316892120032
1107	10-11	-0.0027774040495387453
1107	12-13	-0.09157045113290252
1107	14-15	-0.10625343357315131
1107	16-17	-0.1202533151419658
1107	18-19	-0.06970009097967278
1107	20-21	-0.09821659258464166
1107	22-23	-0.09216195212565026
1107	24-25	-0.08375743659759394
1107	26-27	-0.08662276558280269
1107	28-29	-0.06258572938503448
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2216	28-29	-0.37725331364391934
2216	30-31	-0.33408966026591713
2216	32-33	-0.3281157738650933
2216	34-35	-0.33435481985974036
2216	36-37	-0.33712641692000034
2216	38-39	-0.36607227114386376
2216	40-41	-0.33887423187816523
2216	42-43	-0.35464724457324337
2216	44-45	-0.30749723972770227
2216	46-47	-0.23124631263959117
2216	48-49	-0.17837092318490733
2216	50-51	-0.21145530403502022
2216	52-53	-0.2002920969517703
2216	54-55	-0.28214710093784134
2216	56-57	-0.28380595116743734
2216	58-59	-0.36178726098624736
2216	60-61	-0.2558681453663496
2216	62-63	-0.22927082152956046
2216	64-65	-0.24143546344743783
2216	66-67	-0.24102626970748986
2216	68-69	-0.25642904328553584
2216	70-71	-0.2852857909627815
2216	72-73	-0.19158726646615776
2216	74-75	-0.1814847080124764
2216	76-77	-0.2782979485088859
2216	78-79	-0.27673635254594586
2216	80-81	-0.21140625952433822
2216	82-83	-0.20637847531075693
2216	84-85	-0.1994617023995957
2216	86-87	-0.2679276754128068
2216	88-89	-0.2886376868212537
2216	90-91	-0.2885873509527883
2216	92-93	-0.24482964947496555
2216	94-95	-0.222463262309887
2216	96-97	-0.21806665931077873
2216	98-99	-0.2292083407256733
2216	100	-0.2120154731220083
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	24856.0
3	4400.0
4	2809.0
5	2388.0
6	2034.0
7	2382.0
8	2705.0
9	3037.0
10	3387.0
11	3823.0
12	4138.0
13	4610.0
14	5153.0
15	5556.0
16	5628.0
17	5723.0
18	5693.0
19	5271.0
20	5241.0
21	5538.0
22	6364.0
23	8737.0
24	13300.0
25	18855.0
26	20038.0
27	17155.0
28	17684.0
29	22992.0
30	28401.0
31	34289.0
32	45622.0
33	62745.0
34	88983.0
35	135836.0
36	254381.0
37	559983.0
38	970655.0
39	447367.0
40	1117.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.94492091226812	19.930290918904237	13.308665171463494	23.816122997364154
2	15.198493895633774	20.239503092017415	40.69637015820609	23.865632854142717
3	18.08936601537781	25.59630310972229	30.193020625080504	26.121310249819395
4	11.981986237737573	16.75937735647689	39.55011083642573	31.708525569359807
5	12.753327218895363	38.281957240692485	35.9573043796849	13.007411160727253
6	30.67994665987555	38.627093480946854	18.015200256346034	12.677759602831559
7	27.119686376351876	32.64273118707614	22.902019195351194	17.335563241220786
8	24.66959551031917	37.221712624883416	20.549343598979313	17.5593482658181
9	24.652393433538435	17.29006816391245	21.240629386188935	36.81690901636018
10-11	23.54205905181032	27.283189551346677	29.317443582691304	19.8573078141517
12-13	24.205493419263156	24.503014126679908	29.217796387665864	22.073696066391076
14-15	21.44750437789312	26.546623796462036	26.206957714563995	25.79891411108085
16-17	21.362984368658953	29.941155067941054	26.763616199499012	21.93224436390098
18-19	21.30071866234065	29.00991335609226	28.576693817376782	21.11267416419031
20-21	22.416436702688284	28.310313611097747	28.025995040650052	21.247254645563917
22-23	21.890117886388623	28.18118767978352	28.033836253453547	21.894858180374307
24-25	21.87445715464277	28.283482964036676	28.08040085319327	21.761659028127283
26-27	21.673713811517622	28.260514391428952	28.045339158568805	22.020432638484618
28-29	21.644985902809676	28.310810598064425	28.148217988497642	21.895985510628257
30-31	21.957789037548494	28.77413493136092	27.70091349680953	21.567162534281056
32-33	21.54854665981389	28.393182583918815	28.246569792481935	21.811700963785363
34-35	21.64271863272836	28.168509778445266	28.380125668143325	21.80864592068305
36-37	21.981035017737476	28.45177614366567	27.797600744690566	21.769588093906293
38-39	21.862501777573552	28.614395188578474	27.918810760000373	21.6042922738476
40-41	21.636733710820728	28.365933642886286	28.18997575244299	21.80735689384999
42-43	21.57456982127856	28.239618017701613	28.159895531710326	22.025916629309506
44-45	21.682547100838367	28.320496289512477	28.144644297630983	21.852312312018174
46-47	21.681568933090308	28.43999934935039	28.075932908230595	21.802498809328707
48-49	21.73889165814029	28.33923621131429	28.07901985990503	21.842852270640396
50-51	21.699929898499747	28.305929383582534	28.070333772692035	21.92380694522568
52-53	21.769763933994987	28.160728752870195	28.112962151921227	21.95654516121359
54-55	21.84301326219864	28.564972320573446	27.87856591677213	21.713448500455783
56-57	21.56788362173075	28.328183473190204	28.03876290722504	22.065169997854007
58-59	21.51706722932463	28.007368095558576	28.37451360914954	22.101051065967262
60-61	21.724816841248074	28.426042411992064	28.02097600366309	21.828164743096774
62-63	21.66155613435278	28.409120323827953	27.923060079012117	22.006263462807148
64-65	21.617433066789797	28.155637640704906	28.125555299039462	22.101373993465838
66-67	21.466746466379185	28.247016955223547	28.00740794116742	22.278828637229854
68-69	21.847663355253953	28.315114733454593	28.02919056946971	21.808031341821742
70-71	21.724931059879044	28.225004604169936	28.053920120581154	21.996144215369863
72-73	21.60845186839971	28.31416490035149	28.119696791354905	21.957686439893894
74-75	21.637262640309178	28.37948377661347	27.96095452652589	22.02229905655147
76-77	21.730660691601752	28.365368386643276	27.861532732876853	22.042438188878116
78-79	21.84008784160527	28.366241287373434	27.994457079973216	21.799213791048075
80-81	21.823138470213475	28.10169846660355	28.138619752555677	21.936543310627297
82-83	21.63402518843932	28.234932593492086	28.2594182932313	21.871623924837298
84-85	21.639465544516533	28.171110405686306	28.001213797059776	22.188210252737385
86-87	21.81421100295822	28.35390072732984	27.901947416637608	21.929940853074335
88-89	21.609339672053736	28.114920360456225	28.0395306165931	22.236209350896942
90-91	21.66199533964087	28.162550567430305	28.045399351392593	22.130054741536227
92-93	21.869892506983888	28.211721811389197	27.957773453713504	21.96061222791342
94-95	21.632001705774336	28.052123931823914	28.10331827293163	22.212556089470116
96-97	21.67629020573415	28.095907940536836	28.042656244557403	22.185145609171606
98-99	21.589744437755673	28.24710976506935	27.94208658443349	22.221059212741487
100	21.787287881256077	28.03765511218883	27.808327091140473	22.36672991541462
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	391.0
1	371.0
2	363.5
3	337.0
4	265.0
5	221.0
6	202.0
7	199.0
8	204.0
9	206.0
10	204.5
11	199.0
12	209.5
13	241.5
14	285.0
15	332.5
16	446.0
17	644.0
18	875.0
19	1285.5
20	1974.0
21	2872.0
22	4292.0
23	6593.0
24	9850.5
25	13983.0
26	19031.5
27	25498.0
28	33488.0
29	41974.5
30	50109.0
31	58271.5
32	65912.0
33	74221.5
34	82080.5
35	88007.0
36	93214.0
37	97579.0
38	100719.5
39	101924.0
40	102619.5
41	103345.5
42	103693.0
43	103689.5
44	101947.0
45	100394.0
46	98284.5
47	94126.0
48	92258.0
49	90649.0
50	87081.5
51	83079.0
52	77644.0
53	74160.0
54	72043.5
55	68192.0
56	62527.5
57	57633.5
58	51810.0
59	43548.0
60	37184.0
61	32429.5
62	28104.5
63	23238.0
64	18513.0
65	15153.5
66	12430.5
67	9869.5
68	7984.5
69	6560.0
70	5293.0
71	3944.5
72	2923.5
73	2269.0
74	1703.5
75	1188.5
76	736.0
77	509.0
78	387.5
79	265.0
80	180.5
81	109.0
82	73.0
83	51.0
84	34.0
85	24.0
86	19.5
87	16.5
88	13.0
89	9.0
90	4.5
91	6.5
92	9.5
93	5.0
94	1.0
95	3.0
96	5.0
97	3.0
98	2.5
99	3.0
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.32596726825507644
2	0.11532504382841369
3	0.06198240147526511
4	0.03711248756504305
5	0.04054040818839292
6	0.008394907649020105
7	0.005701541444959488
8	0.014341300567076012
9	0.016509985043072873
10-11	0.01943071332929445
12-13	0.014673598994849725
14-15	0.016807304688975667
16-17	0.018049051445393226
18-19	0.017839178754167722
20-21	0.02233395222458057
22-23	0.014113938484915052
24-25	0.023523230808191752
26-27	0.01776922119042589
28-29	0.017664284844813135
30-31	0.018153987791005976
32-33	0.023330847507901705
34-35	0.013816618839012255
36-37	0.008359928867149187
38-39	0.012050190354530942
40-41	0.008307460694342812
42-43	0.007328054801957132
44-45	0.007765289575343596
46-47	0.006803373073893376
48-49	0.0035328569689626276
50-51	0.0057365202268304044
52-53	0.0064360958642487465
54-55	0.0025884298584478655
56-57	0.0021162163031904846
58-59	0.0022386420397386944
60-61	0.002990685849963412
62-63	0.0025884298584478655
64-65	0.0019588117847713577
66-67	0.001818896657287689
68-69	0.0026583874221896998
70-71	0.001906343611964982
72-73	0.0018363860482231479
74-75	0.001941322393835899
76-77	0.0037252402692526713
78-79	0.0032880054958662076
80-81	0.003200558541188915
82-83	0.0022561314306741532
84-85	0.0027458343768669924
86-87	0.0022036632578677773
88-89	0.5938522692134951
90-91	0.6033664978823845
92-93	0.6001134711683892
94-95	0.5864192780659252
96-97	0.5846003814086376
98-99	0.5818020788589642
100	0.582711527187608
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2858876.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.95350963440785
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.56864102216234	50.38355471960946
2	13.755310847280489	17.869114312862163
3	4.2328698856321125	8.24819264692801
4	1.6427318566049447	4.268047978989519
5	0.8825675981644087	2.8662931495194064
6	0.5063639019513235	1.9734067550346988
7	0.30558136404172986	1.3894007451365997
8	0.21528505970202985	1.1186816159599897
9	0.14678575236280436	0.8580824802262098
>10	0.695855058797628	7.673390904570587
>50	0.033689251776328207	1.5033672408689687
>100	0.013671392791094757	1.5705739111473989
>500	6.470087329039606E-4	0.27789353914695886
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	1.0493634561275131E-4	0.0
10-11	0.0	0.0	0.0	1.0493634561275131E-4	0.0
12-13	0.0	0.0	0.0	1.0493634561275131E-4	0.0
14-15	0.0	0.0	0.0	1.0493634561275131E-4	0.0
16-17	0.0	0.0	0.0	1.0493634561275131E-4	0.0
18-19	0.0	0.0	0.0	1.0493634561275131E-4	0.0
20-21	0.0	0.0	0.0	1.0493634561275131E-4	0.0
22-23	0.0	0.0	0.0	1.2242573654820985E-4	0.0
24-25	0.0	0.0	0.0	2.2736208216096116E-4	0.0
26-27	0.0	0.0	0.0	3.847666005800881E-4	0.0
28-29	0.0	0.0	0.0	0.001241746756417557	0.0
30-31	0.0	0.0	0.0	0.005176859716895731	0.0
32-33	0.0	0.0	0.0	0.013974023357431382	0.0
34-35	0.0	0.0	0.0	0.027073577168089835	0.0
36-37	0.0	0.0	0.0	0.05064927614908796	0.0
38-39	0.0	0.0	0.0	0.09015781027228884	0.0
40-41	0.0	0.0	0.0	0.13631231295096394	0.0
42-43	0.0	0.0	0.0	0.18029813115364218	0.0
44-45	0.0	0.0	0.0	0.23136715268518115	0.0
46-47	0.0	0.0	0.0	0.29637521879228057	0.0
48-49	0.0	0.0	0.0	0.3677844019817579	0.0
50-51	0.0	6.99575637418342E-5	0.0	0.4450700205255492	0.0
52-53	0.0	6.99575637418342E-5	0.0	0.5229152995792752	0.0
54-55	0.0	6.99575637418342E-5	0.0	0.6053777778399623	0.0
56-57	0.0	6.99575637418342E-5	0.0	0.7071835224752665	0.0
58-59	0.0	6.99575637418342E-5	0.0	0.8237503130600978	0.0
60-61	0.0	6.99575637418342E-5	0.0	0.9450217498065674	0.0
62-63	0.0	6.99575637418342E-5	0.0	1.070070894995096	0.0
64-65	0.0	6.99575637418342E-5	0.0	1.2002968999005204	0.0
66-67	0.0	6.99575637418342E-5	0.0	1.3507581301182703	0.0
68-69	0.0	6.99575637418342E-5	0.0	1.5235008443877942	0.0
70-71	0.0	6.99575637418342E-5	0.0	1.7082937490118495	0.0
72-73	0.0	1.0493634561275131E-4	0.0	1.9037202033246632	0.0
74-75	0.0	1.0493634561275131E-4	0.0	2.1028893872976653	0.0
76-77	0.0	1.0493634561275131E-4	0.0	2.3156653174184543	0.0
78-79	0.0	1.0493634561275131E-4	0.0	2.5598347042683907	0.0
80-81	0.0	1.0493634561275131E-4	0.0	2.824729019376846	0.0
82-83	0.0	1.0493634561275131E-4	0.0	3.1002044160012536	0.0
84-85	0.0	1.0493634561275131E-4	0.0	3.3772538578098525	0.0
86-87	0.0	1.0493634561275131E-4	0.0	3.6676127261203355	0.0
88	0.0	1.0493634561275131E-4	0.0	3.9067101895989893	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	3135	0.0	31.552607	1
TCAACGC	3040	0.0	31.058016	4
ATCAACG	3160	0.0	29.884348	3
GGTATCA	2370	0.0	29.414864	1
CAACGCA	3240	0.0	28.995367	5
GTACATG	7530	0.0	28.837624	1
AACGCAG	3390	0.0	27.84413	6
TACATGG	7590	0.0	26.936077	2
ACATGGG	7470	0.0	25.975523	3
TATCAAC	3840	0.0	25.212904	2
GCGTTAG	150	1.1414431E-6	25.121885	1
ACGCAGA	3990	0.0	23.068558	7
CGCAGAG	4190	0.0	21.631197	8
CATGGGG	5350	0.0	19.579533	4
GAGTACA	5250	0.0	17.944202	1
ACGCGTT	105	2.1599692E-5	17.8899	10-11
GACGCGT	110	3.2210024E-5	17.076725	10-11
GTATATA	2310	0.0	16.720734	1
GCAGAGT	5375	0.0	16.600164	9
CGCGACG	85	0.0019314835	16.573307	50-51
>>END_MODULE