FastQCFastQC Report
Wed 25 May 2016
SRR1033117_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033117_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2858876
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52000.18188966572876894No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42750.1495342924981706No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42500.045.4003261
GTATCAA69700.043.347721
ATCAACG81350.036.4659233
TCAACGC81650.036.2167854
CAACGCA83250.035.4642565
AACGCAG85850.034.432926
TATCAAC86800.034.230472
ACGCAGA98900.029.4617777
CGCAGAG98750.029.3611878
GCAGAGT111350.026.1231739
GTACATG75650.025.3813841
ACATGGG71350.024.115853
TACATGG76500.023.9672742
ACCGTCC2704.7293724E-1122.6258628
GAGTACT77150.021.83628712-13
GTGGTAT21350.021.8223421
AGAGTAC105600.020.60357710-11
CATGGGG52000.019.8900014
CAGAGTA116550.019.85726710-11
AGTACTT81950.019.52511812-13
Produced by FastQC (version 0.11.5)