##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1033117_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2858876 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.784853208044 34.0 31.0 34.0 31.0 34.0 2 32.878229066248416 34.0 31.0 34.0 31.0 34.0 3 32.96471970102935 34.0 31.0 34.0 31.0 34.0 4 36.38452979422682 37.0 37.0 37.0 35.0 37.0 5 36.3233389625853 37.0 37.0 37.0 35.0 37.0 6 36.40733630979448 37.0 37.0 37.0 35.0 37.0 7 36.37957994680427 37.0 37.0 37.0 35.0 37.0 8 36.37808775196965 37.0 37.0 37.0 35.0 37.0 9 38.20499699882051 39.0 39.0 39.0 37.0 39.0 10-11 38.18386316860193 39.0 39.0 39.0 37.0 39.0 12-13 38.15001997288445 39.0 39.0 39.0 37.0 39.0 14-15 39.69636231162177 41.0 40.0 41.0 37.0 41.0 16-17 39.71207408086255 41.0 40.0 41.0 37.0 41.0 18-19 39.725309002559044 41.0 40.0 41.0 37.0 41.0 20-21 39.716176917082095 41.0 40.0 41.0 37.0 41.0 22-23 39.648857103281145 41.0 40.0 41.0 37.0 41.0 24-25 39.60018587724686 41.0 40.0 41.0 37.0 41.0 26-27 39.50323658668651 41.0 39.5 41.0 36.5 41.0 28-29 39.415280515839086 41.0 39.0 41.0 36.0 41.0 30-31 39.32492122778322 41.0 39.0 41.0 36.0 41.0 32-33 39.36065904922074 41.0 39.0 41.0 36.0 41.0 34-35 39.30798345223788 41.0 39.0 41.0 35.5 41.0 36-37 39.22943527456245 41.0 39.0 41.0 35.0 41.0 38-39 39.14689514340601 41.0 39.0 41.0 35.0 41.0 40-41 39.03755776745826 41.0 39.0 41.0 35.0 41.0 42-43 38.92588713186581 41.0 39.0 41.0 35.0 41.0 44-45 38.78825874224695 41.0 39.0 41.0 35.0 41.0 46-47 38.590431519240425 41.0 39.0 41.0 35.0 41.0 48-49 38.417946598593296 41.0 39.0 41.0 35.0 41.0 50-51 38.293778044238366 41.0 38.5 41.0 35.0 41.0 52-53 38.16634229676278 40.5 38.0 41.0 34.0 41.0 54-55 38.03945256807221 40.0 38.0 41.0 34.0 41.0 56-57 37.89043683601527 40.0 37.5 41.0 34.0 41.0 58-59 37.711311543417764 40.0 37.0 41.0 34.0 41.0 60-61 37.52556599166945 40.0 37.0 41.0 33.5 41.0 62-63 37.300754212494695 39.5 36.0 41.0 33.0 41.0 64-65 37.05550415617886 39.0 36.0 41.0 33.0 41.0 66-67 36.78034042050092 39.0 35.0 41.0 33.0 41.0 68-69 36.46173181348195 38.5 35.0 41.0 32.0 41.0 70-71 36.12770018706652 37.5 35.0 40.0 32.0 41.0 72-73 35.773421967234675 37.0 35.0 39.5 32.0 41.0 74-75 35.431778433202425 36.5 35.0 39.0 31.5 41.0 76-77 35.08624805692867 36.0 35.0 39.0 31.0 41.0 78-79 34.73883512261462 35.5 35.0 38.0 31.0 39.5 80-81 34.38924056167529 35.0 35.0 37.0 31.0 39.0 82-83 34.064442983885975 35.0 35.0 37.0 31.0 39.0 84-85 33.78500204276086 35.0 34.0 36.0 31.0 38.0 86-87 33.54775985387264 35.0 34.0 36.0 30.5 37.0 88-89 33.324381854966774 35.0 34.0 36.0 30.0 37.0 90-91 33.12237047007285 35.0 34.0 35.0 30.0 36.5 92-93 32.958687959883534 35.0 34.0 35.0 30.0 36.0 94-95 32.81112262301688 35.0 34.0 35.0 30.0 36.0 96-97 32.69300557281953 35.0 34.0 35.0 30.0 36.0 98-99 32.5902776126002 35.0 34.0 35.0 30.0 36.0 100 32.49895343484642 35.0 34.0 35.0 29.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.12155508739438403 1101 2 0.14386170074914872 1101 3 0.11223003250969299 1101 4 0.10071676176722377 1101 5 0.08480886848199276 1101 6 0.1115052426208365 1101 7 0.11639511163899385 1101 8 0.12715475428935719 1101 9 0.14314237886880932 1101 10-11 0.1512060134797153 1101 12-13 0.1723263059989577 1101 14-15 0.17122082061057853 1101 16-17 0.20342148481743294 1101 18-19 0.24075924808875016 1101 20-21 0.22263386934303497 1101 22-23 0.25420893929371147 1101 24-25 0.2012610185909125 1101 26-27 0.23483234135970577 1101 28-29 0.279694208568543 1101 30-31 0.3329325179339975 1101 32-33 0.24685099363549767 1101 34-35 0.23457773531705328 1101 36-37 0.20935859503387633 1101 38-39 0.2506962292599013 1101 40-41 0.2867073527596986 1101 42-43 0.23956731649105478 1101 44-45 0.21688199743398684 1101 46-47 0.20708430162188307 1101 48-49 0.1047254740722181 1101 50-51 0.09068519845050815 1101 52-53 0.21655162358431568 1101 54-55 0.22125550458486032 1101 56-57 0.23155140770995075 1101 58-59 0.23538467206009273 1101 60-61 0.22516129854864175 1101 62-63 0.1770320605322766 1101 64-65 0.1485542893663805 1101 66-67 0.18090881482384447 1101 68-69 0.20502990062565374 1101 70-71 0.19082321822958903 1101 72-73 0.20821329120080634 1101 74-75 0.16611780431883716 1101 76-77 0.1692751635002736 1101 78-79 0.1605755931138404 1101 80-81 0.10676950600956303 1101 82-83 0.14598259199881625 1101 84-85 0.25182841857923677 1101 86-87 0.2242217576360872 1101 88-89 0.17802879510861658 1101 90-91 0.21043107827819085 1101 92-93 0.1367275657125191 1101 94-95 0.20324374788495447 1101 96-97 0.24291031722498957 1101 98-99 0.31957153582061437 1101 100 0.31616132748091985 1102 1 0.14247978566442043 1102 2 0.1712192771913621 1102 3 0.15733755298523988 1102 4 0.09140056867655488 1102 5 0.08449170255140359 1102 6 0.11251605991984803 1102 7 0.11855853275488215 1102 8 0.17546127262630762 1102 9 0.2195754349995056 1102 10-11 0.17169793725775406 1102 12-13 0.17806340503776852 1102 14-15 0.22186858962970746 1102 16-17 0.17497336436890976 1102 18-19 0.1582190230150644 1102 20-21 0.17849641624413692 1102 22-23 0.19977052480613366 1102 24-25 0.22043772572895648 1102 26-27 0.20433531223537216 1102 28-29 0.23836395443345992 1102 30-31 0.2696328974240956 1102 32-33 0.26789710346221085 1102 34-35 0.22420323708271184 1102 36-37 0.24057442183949007 1102 38-39 0.2474163763310102 1102 40-41 0.24020985372170855 1102 42-43 0.2291972672197531 1102 44-45 0.2707052299447881 1102 46-47 0.3080003882469313 1102 48-49 0.274822968261482 1102 50-51 0.2909814478070203 1102 52-53 0.35280975680458226 1102 54-55 0.3310353763412408 1102 56-57 0.3698240507395951 1102 58-59 0.3708921556028031 1102 60-61 0.37405687248808306 1102 62-63 0.30733193611606424 1102 64-65 0.2521240857302445 1102 66-67 0.2996884229952883 1102 68-69 0.35000100557785885 1102 70-71 0.2881840030834155 1102 72-73 0.2904833546056125 1102 74-75 0.3267767589337396 1102 76-77 0.2271037523046715 1102 78-79 0.23715984951029156 1102 80-81 0.22893332430824387 1102 82-83 0.2244443752773222 1102 84-85 0.26286020274494604 1102 86-87 0.2829569999743313 1102 88-89 0.27689149185594175 1102 90-91 0.269310976393065 1102 92-93 0.2765988372235739 1102 94-95 0.2094782925742109 1102 96-97 0.194192044134482 1102 98-99 0.2253434334877653 1102 100 0.254388055922405 1103 1 0.06328332561812999 1103 2 0.06300115855518129 1103 3 0.07262648098942748 1103 4 0.040991782232048024 1103 5 -0.051140383299639325 1103 6 0.009495477685554476 1103 7 0.08313477120950097 1103 8 0.09692922885680844 1103 9 0.052167620677565196 1103 10-11 0.06536871113352305 1103 12-13 0.06129247553980122 1103 14-15 0.12338245277795323 1103 16-17 0.11486193672331524 1103 18-19 0.09494719539320329 1103 20-21 0.1598719878538688 1103 22-23 0.13862098910545484 1103 24-25 0.11662365330715119 1103 26-27 0.16070802221364033 1103 28-29 0.040607128325177655 1103 30-31 0.1432023513619427 1103 32-33 0.1826176809440625 1103 34-35 0.21640024455278706 1103 36-37 0.22748947709070677 1103 38-39 0.21280999042701154 1103 40-41 0.2667930078011338 1103 42-43 0.2160064251554914 1103 44-45 0.2839223305983438 1103 46-47 0.2985515184357226 1103 48-49 0.2682509857940829 1103 50-51 0.25611531144468813 1103 52-53 0.2593095158234675 1103 54-55 0.2370740382306522 1103 56-57 0.23952418443033707 1103 58-59 0.23498796049729265 1103 60-61 0.225229598961171 1103 62-63 0.20127244611087747 1103 64-65 0.17178654820774142 1103 66-67 0.2591790322518861 1103 68-69 0.2434990645071835 1103 70-71 0.2256473851416274 1103 72-73 0.2633951233416596 1103 74-75 0.202999963846338 1103 76-77 0.20964696817931383 1103 78-79 0.24787699069205615 1103 80-81 0.2561443422150589 1103 82-83 0.18279335229876637 1103 84-85 0.23418955245715267 1103 86-87 0.2546752356954656 1103 88-89 0.2619547926234844 1103 90-91 0.1523009766168144 1103 92-93 0.13700734443941798 1103 94-95 0.13898994506976692 1103 96-97 0.17836434042284566 1103 98-99 0.25489670523093366 1103 100 0.20665148159700664 1104 1 -0.01128789267081487 1104 2 0.04132509126809225 1104 3 0.015732651290086608 1104 4 0.02782180692782532 1104 5 -0.012349888398986764 1104 6 -0.02452102534987688 1104 7 0.05418905637738192 1104 8 0.08262546670451343 1104 9 0.03791684308771437 1104 10-11 0.09194274588310947 1104 12-13 0.10886764531807103 1104 14-15 0.10191666797310006 1104 16-17 0.08692417040344935 1104 18-19 0.1159412934096764 1104 20-21 0.10495576555382513 1104 22-23 0.09787710240631498 1104 24-25 0.10156030777909564 1104 26-27 0.0011679861675730763 1104 28-29 0.1205749070881339 1104 30-31 0.07103944701314902 1104 32-33 0.10590783185899966 1104 34-35 0.11920275535873515 1104 36-37 0.06996699383672222 1104 38-39 0.07248555104921905 1104 40-41 0.13519053110399426 1104 42-43 0.0925565340636112 1104 44-45 0.10352955769266003 1104 46-47 0.12405493387029054 1104 48-49 0.17976655952997334 1104 50-51 0.21318332789308414 1104 52-53 0.21751028426520236 1104 54-55 0.1936163484734834 1104 56-57 0.23417853880930295 1104 58-59 0.16711266628809085 1104 60-61 0.21710454049188144 1104 62-63 0.25997458097480575 1104 64-65 0.2470124156022777 1104 66-67 0.29598985004377454 1104 68-69 0.25352577203819493 1104 70-71 0.19417352019100065 1104 72-73 0.2414340570604594 1104 74-75 0.19896278855270566 1104 76-77 0.16437497110008792 1104 78-79 0.20018798689290662 1104 80-81 0.2475790565413334 1104 82-83 0.28666855212922115 1104 84-85 0.26669433969514955 1104 86-87 0.22917372439361117 1104 88-89 0.25545818156577127 1104 90-91 0.2765886861024569 1104 92-93 0.2575254920660015 1104 94-95 0.1566969305048076 1104 96-97 0.19932966947767738 1104 98-99 0.24983481677834618 1104 100 0.25114609579902236 1105 1 -0.005011377163533837 1105 2 -0.00996430309822216 1105 3 -0.0035688449344846163 1105 4 -0.0011467174253141366 1105 5 0.005750074316360099 1105 6 0.005059251922709507 1105 7 -0.032733081313985224 1105 8 -0.08093683993471501 1105 9 -0.05886495061715635 1105 10-11 -0.09861026236220738 1105 12-13 0.0022292694489252085 1105 14-15 -0.029611234324029567 1105 16-17 -0.011467751065396214 1105 18-19 0.01247464609687654 1105 20-21 0.007715199851723753 1105 22-23 0.06584833345446839 1105 24-25 -0.009694249629312424 1105 26-27 0.01114157383346992 1105 28-29 -0.014931068417837423 1105 30-31 0.08341060861507543 1105 32-33 0.013628515733529412 1105 34-35 0.001765349459795118 1105 36-37 -0.010110108548069263 1105 38-39 -0.051059194803215746 1105 40-41 0.047722614405309116 1105 42-43 0.0370542585613336 1105 44-45 0.10634756589527683 1105 46-47 0.10110571784415612 1105 48-49 0.07160079367189809 1105 50-51 0.026693976788344287 1105 52-53 0.04952450935634545 1105 54-55 0.028737637440919173 1105 56-57 -0.02943061556908333 1105 58-59 -0.06671208445973775 1105 60-61 -0.06669931946581897 1105 62-63 -0.07273837558430785 1105 64-65 -0.15304943138264804 1105 66-67 -0.1439723245337845 1105 68-69 -0.16943107168788174 1105 70-71 -0.09877213813926744 1105 72-73 -0.15003131517414658 1105 74-75 -0.0929302756480439 1105 76-77 -0.10819963378222042 1105 78-79 -0.06135497157313097 1105 80-81 -0.06284800927034695 1105 82-83 -0.09621759306461541 1105 84-85 -0.14174132720205534 1105 86-87 -0.1821960169761354 1105 88-89 -0.13766297078614542 1105 90-91 -0.16382087584555904 1105 92-93 -0.08296902765928849 1105 94-95 -0.11445604757124528 1105 96-97 -0.039522475528315226 1105 98-99 -0.015986911351077993 1105 100 -0.06253543634404934 1106 1 0.04769092036080025 1106 2 0.08677997090546796 1106 3 0.0421516973941749 1106 4 0.023699104796783388 1106 5 0.01511437019079409 1106 6 0.0354268064830876 1106 7 0.029909003148738122 1106 8 0.03349368482609094 1106 9 0.06492806115961258 1106 10-11 0.034490312909603915 1106 12-13 0.049789914169934946 1106 14-15 0.10721084800095326 1106 16-17 0.07813610892895895 1106 18-19 0.010343920443155241 1106 20-21 0.03597647575471541 1106 22-23 0.07030440148947292 1106 24-25 0.06433300931973918 1106 26-27 0.06491241339046638 1106 28-29 0.042555303477975315 1106 30-31 0.07763248429169067 1106 32-33 0.17137201960201764 1106 34-35 0.20480697238954093 1106 36-37 0.1836489734288449 1106 38-39 0.22414072516849615 1106 40-41 0.14360441920755562 1106 42-43 0.09634132166033993 1106 44-45 0.10188760607386627 1106 46-47 0.14012383657652805 1106 48-49 0.13817199280802583 1106 50-51 0.11138177055655518 1106 52-53 0.1160627379357635 1106 54-55 0.13563445850918754 1106 56-57 0.18582735231545655 1106 58-59 0.13671394830252837 1106 60-61 0.15766605898300412 1106 62-63 0.15688491593515863 1106 64-65 0.1571635754060523 1106 66-67 0.13854779901672742 1106 68-69 0.07075795402257512 1106 70-71 0.15309153422712996 1106 72-73 0.18044986858669176 1106 74-75 0.16272533962420255 1106 76-77 0.11231613754518577 1106 78-79 0.1350232826591835 1106 80-81 0.1625386616352671 1106 82-83 0.12132101647135585 1106 84-85 0.1294589874297003 1106 86-87 0.1615845794538444 1106 88-89 0.12123732548295152 1106 90-91 0.1217461875823318 1106 92-93 0.06567224555782047 1106 94-95 0.10560945503104335 1106 96-97 0.14462271551180095 1106 98-99 0.13625615537661417 1106 100 0.24468333412551857 1107 1 -0.02464519793299047 1107 2 0.011324157425406156 1107 3 -0.028756758394841597 1107 4 0.009731547127067586 1107 5 -0.06885144089871176 1107 6 -0.05299294302829338 1107 7 -0.10584963704800288 1107 8 0.012046813116484145 1107 9 -0.025938913084530668 1107 10-11 -0.015019887320086411 1107 12-13 0.004154071590086517 1107 14-15 0.023470032243132266 1107 16-17 -0.020426436489806576 1107 18-19 -0.04078883056261162 1107 20-21 -0.03641000632875091 1107 22-23 -0.0452028317781199 1107 24-25 0.010732061908541368 1107 26-27 0.051735265353656246 1107 28-29 0.02858491251409845 1107 30-31 0.06763543091761193 1107 32-33 0.0709418302225231 1107 34-35 0.04860603537944286 1107 36-37 0.020792999144781277 1107 38-39 0.004507495362496172 1107 40-41 0.05609537517371166 1107 42-43 -0.028325607399374064 1107 44-45 0.009394612254254753 1107 46-47 0.0377946251654393 1107 48-49 0.013022265127311528 1107 50-51 -0.04033901344082835 1107 52-53 -0.04418914774065996 1107 54-55 -0.09621063984425149 1107 56-57 -0.1294809784061215 1107 58-59 -0.1607747362620131 1107 60-61 -0.1490033989638775 1107 62-63 -0.0998082516527603 1107 64-65 -0.17428441691110663 1107 66-67 -0.15769180001336025 1107 68-69 -0.18995004435095808 1107 70-71 -0.22920471458672864 1107 72-73 -0.21427911332227723 1107 74-75 -0.19639188853075495 1107 76-77 -0.1409465463183892 1107 78-79 -0.16796524954298064 1107 80-81 -0.13748118731150072 1107 82-83 -0.07275102779116338 1107 84-85 -0.09436026238391548 1107 86-87 -0.1229411488166221 1107 88-89 -0.11219791287970793 1107 90-91 -0.13404350340006488 1107 92-93 -0.14097210463648935 1107 94-95 -0.10737461592438535 1107 96-97 0.007737377496638942 1107 98-99 0.0509931721900756 1107 100 0.04948986144212597 1108 1 0.03522817108662224 1108 2 0.020899157049058203 1108 3 -0.02388354349852051 1108 4 -0.029840199010045865 1108 5 -0.04436129972074099 1108 6 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2214 16-17 -0.07097250683419531 2214 18-19 -0.0412294038756329 2214 20-21 -0.10457231646709175 2214 22-23 -0.1485705229811387 2214 24-25 -0.10527575650682763 2214 26-27 -0.12998113638555964 2214 28-29 -0.07972561484562846 2214 30-31 -0.013670388413025591 2214 32-33 -0.012764220910469248 2214 34-35 -0.0541327969166403 2214 36-37 -0.08479180991921709 2214 38-39 -0.15573711771786236 2214 40-41 -0.07424831085634054 2214 42-43 -0.11140696502882719 2214 44-45 -0.16965080646191666 2214 46-47 -0.09604757849583478 2214 48-49 -0.17971548978016472 2214 50-51 -0.10810914382918924 2214 52-53 -0.17268206079820914 2214 54-55 -0.1615697739046169 2214 56-57 -0.1945823956822892 2214 58-59 -0.21108843325971804 2214 60-61 -0.28685716690822005 2214 62-63 -0.2566171791699716 2214 64-65 -0.15514705604143586 2214 66-67 -0.291468854893445 2214 68-69 -0.2468367011036534 2214 70-71 -0.13278793499394226 2214 72-73 -0.26819043179038715 2214 74-75 -0.21453568368501408 2214 76-77 -0.20626966602020502 2214 78-79 -0.36594165445163895 2214 80-81 -0.26944512165762546 2214 82-83 -0.24890584376036173 2214 84-85 -0.2933597418489029 2214 86-87 -0.18982283142243261 2214 88-89 -0.302948232141496 2214 90-91 -0.2343967998113925 2214 92-93 -0.23517232799571275 2214 94-95 -0.2660180760210835 2214 96-97 -0.3857853640614266 2214 98-99 -0.5320304314192867 2214 100 -0.6457681965307422 2215 1 -0.054347879639131236 2215 2 -0.05489560266067883 2215 3 -0.0712205152770693 2215 4 -0.06501882873548936 2215 5 -0.028672282887846734 2215 6 -0.045417856258772815 2215 7 -0.08296222552661447 2215 8 -0.06376984374387717 2215 9 -0.0891166024017025 2215 10-11 -0.08949191202923146 2215 12-13 -0.07776242701767444 2215 14-15 -0.15557481298038311 2215 16-17 -0.11536351198073191 2215 18-19 -0.14119465914243534 2215 20-21 -0.140037825941576 2215 22-23 -0.1395677116368077 2215 24-25 -0.1412447025168717 2215 26-27 -0.13917840960704098 2215 28-29 -0.1192284919432538 2215 30-31 -0.15898858910656344 2215 32-33 -0.1506986720333856 2215 34-35 -0.16786822441429194 2215 36-37 -0.15396106845081192 2215 38-39 -0.17441434573004955 2215 40-41 -0.20044699180736103 2215 42-43 -0.19312946603735526 2215 44-45 -0.14881437679844112 2215 46-47 -0.17789195764234478 2215 48-49 -0.14993533621535704 2215 50-51 -0.12725767237903796 2215 52-53 -0.17146389662696038 2215 54-55 -0.16513226117983493 2215 56-57 -0.24485848546294164 2215 58-59 -0.2830752082061778 2215 60-61 -0.24260441650296372 2215 62-63 -0.2527053517895652 2215 64-65 -0.18303044507990762 2215 66-67 -0.22447805492353012 2215 68-69 -0.18852817544398448 2215 70-71 -0.14468841758528583 2215 72-73 -0.05656184196077163 2215 74-75 -0.09181201096756553 2215 76-77 -0.144989568662524 2215 78-79 -0.13911520645130082 2215 80-81 -0.1302943991367016 2215 82-83 -0.09647386056686713 2215 84-85 -0.19521146660148503 2215 86-87 -0.1877009503218403 2215 88-89 -0.18000318724902797 2215 90-91 -0.1117125873013407 2215 92-93 -0.08987173832278472 2215 94-95 -0.0642295205181398 2215 96-97 -0.10864089723836656 2215 98-99 -0.08905874286310222 2215 100 -0.11395676809756594 2216 1 -0.06892123360788815 2216 2 -0.09083000447492395 2216 3 -0.146864307043991 2216 4 -0.07760529672308536 2216 5 -0.025586372328191942 2216 6 -0.0533473072845112 2216 7 -0.09437729733994615 2216 8 -0.0768911235403209 2216 9 -0.11351928081732865 2216 10-11 -0.11527042447393399 2216 12-13 -0.10543045707949972 2216 14-15 -0.21093057293808926 2216 16-17 -0.18185812946232716 2216 18-19 -0.10338317857909374 2216 20-21 -0.0866979363130227 2216 22-23 -0.16653732008981592 2216 24-25 -0.23224847274371996 2216 26-27 -0.21996344144697844 2216 28-29 -0.21032266714870218 2216 30-31 -0.15452970129134513 2216 32-33 -0.1474583171554542 2216 34-35 -0.15109110774151446 2216 36-37 -0.259342979789416 2216 38-39 -0.17495010979673964 2216 40-41 -0.18404726681942662 2216 42-43 -0.18574667052734384 2216 44-45 -0.09790220901442126 2216 46-47 -0.17820239369786606 2216 48-49 -0.16857411696346247 2216 50-51 -0.12015299567413962 2216 52-53 -0.033736643269477895 2216 54-55 -0.010285137843638381 2216 56-57 -0.041973406081538656 2216 58-59 -0.019503986580787114 2216 60-61 -0.08952194597474517 2216 62-63 -0.05914894729615128 2216 64-65 -0.0012092495820823501 2216 66-67 -6.949630708774635E-4 2216 68-69 0.013250439844568973 2216 70-71 5.291769018995751E-4 2216 72-73 0.0011301040566848997 2216 74-75 0.037715707234582396 2216 76-77 0.041138034364038845 2216 78-79 0.023839797223224934 2216 80-81 -0.005172599407472944 2216 82-83 -0.0635440107831684 2216 84-85 -0.011569137502604576 2216 86-87 -0.04709377167850448 2216 88-89 -0.045531286830438944 2216 90-91 -0.05561775622831533 2216 92-93 -0.05109633476186559 2216 94-95 -0.037868404824294544 2216 96-97 -0.035606172918207335 2216 98-99 0.01971987218738036 2216 100 0.02722200331365343 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 24.0 3 9.0 4 14.0 5 31.0 6 52.0 7 141.0 8 271.0 9 564.0 10 1040.0 11 1742.0 12 2740.0 13 4198.0 14 5037.0 15 6578.0 16 8366.0 17 12146.0 18 14266.0 19 7827.0 20 5062.0 21 4780.0 22 4904.0 23 5311.0 24 5980.0 25 7501.0 26 9306.0 27 12083.0 28 15410.0 29 19887.0 30 25698.0 31 32756.0 32 42082.0 33 55351.0 34 77342.0 35 120371.0 36 222499.0 37 524898.0 38 1044895.0 39 555358.0 40 2356.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.85481214103033 19.964008941747352 13.496191704901332 23.684987212320983 2 15.511546421971625 20.369756940812938 40.31546240080368 23.80323423641176 3 18.16633530100641 25.673621381270124 30.39834536370238 25.76169795402109 4 12.113815678093568 16.94100602056478 39.29279001338992 31.652388287951734 5 12.4991164538341 37.916902570576966 36.081855915840215 13.502125059748716 6 30.516113009685647 38.714806899229416 17.67736903042111 13.09171106066383 7 26.74446060147288 32.80438401552567 22.924782833330244 17.5263725496712 8 25.02041891391316 36.36584957198109 20.49401354171877 18.11971797238698 9 25.02413539326 16.37491989847744 21.42834656465907 37.172598143603494 10-11 23.689443561808858 26.789593201770494 29.2454655034066 20.275497733014046 12-13 24.71000840889916 24.069372018933315 29.43621898956093 21.78440058260659 14-15 21.698060739319306 25.94637817069873 26.894968751672426 25.460592338309535 16-17 20.904579282207415 29.57006529839 27.46052994253686 22.06482547686573 18-19 20.832300000734556 28.129388748643258 30.085334325799014 20.952976924823176 20-21 21.899270902270683 27.381198065253614 29.809407613341747 20.910123419133953 22-23 21.336052356350585 27.278819953338207 29.813667637912882 21.571460052398326 24-25 21.301498422106047 27.433160747902846 29.87875646146 21.386584368531107 26-27 21.180379626732503 27.41395138473388 29.852689968586848 21.552979019946765 28-29 21.19010533956596 27.474063028829548 29.877659179059773 21.458172452544723 30-31 21.42825275805582 27.941003315248093 29.487914976806913 21.142828949889175 32-33 21.098640344754354 27.507888841422307 29.97667023190867 21.41680058191467 34-35 21.134239027370256 27.401851461792692 30.023274480015505 21.440635030821543 36-37 21.535030319485134 27.688303482100547 29.39070106822763 21.38596513018669 38-39 21.51657874015955 27.85561427818947 29.423439211927523 21.20436776972346 40-41 21.32035811599844 27.64337104893698 29.577739775455846 21.45853105960873 42-43 21.299818785912848 27.55459994965401 29.45179371157904 21.693787552854108 44-45 21.473780073194995 27.680345581421957 29.266365445636332 21.579508899746717 46-47 21.5067326447603 27.882414904579395 29.043771855360127 21.567080595300173 48-49 21.607628584207212 27.902029147846758 28.773183183377533 21.717159084568497 50-51 21.629450394609208 27.92430765420473 28.651293914507225 21.79494803667884 52-53 21.686118811708017 27.847187120132226 28.613665182904967 21.85302888525479 54-55 21.761475880777294 28.1308640874083 28.42999649324403 21.67766353857038 56-57 21.466009609599528 27.946840081600442 28.606730497466078 21.980419811333952 58-59 21.382387668300137 27.631872818501606 28.94866403145188 22.037075481746378 60-61 21.604533421760312 28.009560467543658 28.613595787965533 21.77231032273049 62-63 21.478972819527392 27.937161826904696 28.5748865228517 22.008978830716213 64-65 21.437687437549965 27.674776579075278 28.771396105094276 22.11613987828048 66-67 21.3111955776724 27.76150184523078 28.658433887814937 22.268868689281888 68-69 21.701190885739084 27.831407755282434 28.595371861684388 21.872029497294093 70-71 21.656280546447913 27.745868244496457 28.58719907403764 22.01065213501799 72-73 21.58258886409268 27.845452383117745 28.57418691366489 21.997771839124685 74-75 21.52376794880338 27.891689713436524 28.46621932059994 22.118323017160158 76-77 21.555356369884876 27.883275253706675 28.35855169669755 22.2028166797109 78-79 21.701998163613307 27.940116304490402 28.411560491452057 21.946325040444233 80-81 21.66077445582311 27.658691806766267 28.568442864793266 22.11209087261736 82-83 21.438446738325727 27.795505308669206 28.63774617972924 22.12830177327583 84-85 21.445581181896024 27.748032085471714 28.374627124620126 22.431759608012133 86-87 21.57874182172931 27.8143491234216 28.338611931316255 22.268297123532836 88-89 21.317906058185105 27.621747148179914 28.464910685178367 22.59543610845661 90-91 21.28020637488523 27.626828735079357 28.524769358576364 22.568195531459054 92-93 21.461427753674478 27.702360595975584 28.379479973856853 22.45673167649308 94-95 21.225544584654948 27.543971826689926 28.497493420491132 22.73299016816399 96-97 21.226558969329204 27.54267761176071 28.45683058656619 22.7739328323439 98-99 21.02466144037027 27.674460172459387 28.40932940078548 22.89154898638486 100 21.25461195238968 27.477861928953896 28.265689033032565 23.001837085623862 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 42.0 1 38.0 2 120.5 3 214.0 4 151.5 5 131.0 6 311.0 7 644.5 8 1146.0 9 1427.5 10 1158.0 11 903.5 12 1000.0 13 1170.5 14 1322.0 15 1513.5 16 1714.5 17 1974.5 18 2339.0 19 2763.0 20 3445.0 21 4347.0 22 5709.5 23 7918.5 24 10866.5 25 14695.0 26 19465.0 27 25516.0 28 33210.0 29 41383.0 30 49456.0 31 57527.0 32 64603.5 33 72800.5 34 81142.5 35 87264.5 36 92013.5 37 96210.0 38 99541.0 39 100801.5 40 101455.0 41 102204.0 42 103143.5 43 103435.5 44 101500.0 45 100496.0 46 98642.0 47 94136.5 48 92162.0 49 90664.5 50 86862.0 51 82182.0 52 76612.5 53 73575.0 54 72116.5 55 68501.5 56 62671.5 57 57882.0 58 52116.0 59 43734.0 60 37403.0 61 32599.0 62 28107.0 63 22949.5 64 18154.0 65 14909.0 66 12084.0 67 9513.5 68 7618.5 69 6107.5 70 4817.5 71 3580.5 72 2618.5 73 2015.5 74 1560.0 75 1039.0 76 619.0 77 409.0 78 296.5 79 194.5 80 118.0 81 51.0 82 15.5 83 11.0 84 7.0 85 3.0 86 1.0 87 0.5 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.5 94 0.5 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09132959946496455 2 8.744695467729275E-4 3 0.0 4 2.798302549673368E-4 5 0.03756721172936497 6 2.0987269122550262E-4 7 0.004442305297606472 8 2.448514730964197E-4 9 1.399151274836684E-4 10-11 4.897029461928394E-4 12-13 0.0 14-15 1.0493634561275131E-4 16-17 0.0 18-19 1.0493634561275131E-4 20-21 0.0 22-23 2.0987269122550262E-4 24-25 6.99575637418342E-5 26-27 0.0014516194476430597 28-29 0.009216909022986657 30-31 0.035695846899270904 32-33 0.0253596168564149 34-35 0.02833281331544285 36-37 0.02555200015670494 38-39 0.024922382083028435 40-41 0.030204178145536918 42-43 0.022893612734515244 44-45 0.021704334150904062 46-47 0.01582789879658999 48-49 0.010895890552790678 50-51 0.007870225920956347 52-53 0.005281796062508482 54-55 0.0037951978329945056 56-57 0.0023785571672223626 58-59 0.0011368104108048058 60-61 6.99575637418342E-4 62-63 0.0014166406657721425 64-65 0.0031655797593179978 66-67 6.820862464828835E-4 68-69 0.0013117043201593913 70-71 4.3723477338646375E-4 72-73 5.596605099346736E-4 74-75 5.42171118999215E-4 76-77 4.7221355525738085E-4 78-79 3.49787818709171E-5 80-81 5.946392918055906E-4 82-83 5.07192337128298E-4 84-85 4.1974538245100523E-4 86-87 1.5740451841912698E-4 88-89 0.0 90-91 3.49787818709171E-5 92-93 1.748939093545855E-5 94-95 0.0 96-97 0.0 98-99 0.0 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2858876.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.031084082671626 #Duplication Level Percentage of deduplicated Percentage of total 1 76.45737887174022 48.191914764053394 2 14.375439798621992 18.12199109344654 3 4.384368708261932 8.290545380996768 4 1.8089613766319315 4.560831865311708 5 0.9298304031319865 2.9304109161218355 6 0.5110377318123541 1.9326757345969372 7 0.33968970804899234 1.498770738503795 8 0.23101957716586924 1.164913151446812 9 0.1627584652173149 0.9232958255651245 >10 0.7450738478739692 8.092918703816865 >50 0.0366511251314049 1.5994915483126186 >100 0.016736645953209162 1.909551866039346 >500 8.319003228895024E-4 0.335343007463879 >1k 1.66380064443583E-4 0.2655687299094868 >5k 5.5460021481194333E-5 0.18177667441481263 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5200 0.18188966572876894 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4275 0.1495342924981706 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 3.49787818709171E-5 0.0 10-11 0.0 0.0 0.0 3.49787818709171E-5 0.0 12-13 0.0 0.0 0.0 3.49787818709171E-5 0.0 14-15 0.0 0.0 0.0 6.99575637418342E-5 0.0 16-17 0.0 0.0 0.0 6.99575637418342E-5 0.0 18-19 0.0 0.0 0.0 6.99575637418342E-5 0.0 20-21 0.0 0.0 0.0 8.744695467729276E-5 0.0 22-23 0.0 0.0 0.0 1.2242573654820985E-4 0.0 24-25 0.0 0.0 0.0 2.2736208216096116E-4 0.0 26-27 0.0 0.0 0.0 3.4978781870917105E-4 0.0 28-29 0.0 0.0 0.0 0.00120676797454664 0.0 30-31 0.0 0.0 0.0 0.005159370325960273 0.0 32-33 0.0 0.0 0.0 0.013886576402754088 0.0 34-35 0.0 0.0 0.0 0.027003619604348002 0.0 36-37 0.0 0.0 0.0 0.05047438223973338 0.0 38-39 0.0 0.0 0.0 0.09003538453574061 0.0 40-41 0.0 0.0 0.0 0.13636478112377032 0.0 42-43 0.0 0.0 0.0 0.18043804628112586 0.0 44-45 0.0 0.0 0.0 0.23183936624043855 0.0 46-47 0.0 0.0 0.0 0.2974070928574727 0.0 48-49 0.0 0.0 0.0 0.36921853203846544 0.0 50-51 0.0 3.49787818709171E-5 0.0 0.44680147022815964 0.0 52-53 0.0 6.99575637418342E-5 0.0 0.524926579536853 0.0 54-55 0.0 6.99575637418342E-5 0.0 0.6079312289165393 0.0 56-57 0.0 6.99575637418342E-5 0.0 0.7105239961439391 0.0 58-59 0.0 6.99575637418342E-5 0.0 0.8277378941933823 0.0 60-61 0.0 6.99575637418342E-5 0.0 0.9496564384044639 0.0 62-63 0.0 1.0493634561275131E-4 0.0 1.0749329456751535 0.0 64-65 0.0 1.0493634561275131E-4 0.0 1.2050365248440296 0.0 66-67 0.0 1.0493634561275131E-4 0.0 1.3553053717614896 0.0 68-69 0.0 1.0493634561275131E-4 0.0 1.5286077465409482 0.0 70-71 0.0 1.0493634561275131E-4 0.0 1.7137154602018416 0.0 72-73 0.0 1.0493634561275131E-4 0.0 1.9097540431973965 0.0 74-75 0.0 1.0493634561275131E-4 0.0 2.109675270980623 0.0 76-77 0.0 1.0493634561275131E-4 0.0 2.323867841767184 0.0 78-79 0.0 1.0493634561275131E-4 0.0 2.570485743348085 0.0 80-81 0.0 1.0493634561275131E-4 0.0 2.8386855533433417 0.0 82-83 0.0 1.0493634561275131E-4 0.0 3.116959252517423 0.0 84-85 0.0 1.0493634561275131E-4 0.0 3.3972617210400173 0.0 86-87 0.0 1.0493634561275131E-4 0.0 3.691065999364785 0.0 88 0.0 1.0493634561275131E-4 0.0 3.933364021384628 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4250 0.0 45.400326 1 GTATCAA 6970 0.0 43.34772 1 ATCAACG 8135 0.0 36.465923 3 TCAACGC 8165 0.0 36.216785 4 CAACGCA 8325 0.0 35.464256 5 AACGCAG 8585 0.0 34.43292 6 TATCAAC 8680 0.0 34.23047 2 ACGCAGA 9890 0.0 29.461777 7 CGCAGAG 9875 0.0 29.361187 8 GCAGAGT 11135 0.0 26.123173 9 GTACATG 7565 0.0 25.381384 1 ACATGGG 7135 0.0 24.11585 3 TACATGG 7650 0.0 23.967274 2 ACCGTCC 270 4.7293724E-11 22.625862 8 GAGTACT 7715 0.0 21.836287 12-13 GTGGTAT 2135 0.0 21.822342 1 AGAGTAC 10560 0.0 20.603577 10-11 CATGGGG 5200 0.0 19.890001 4 CAGAGTA 11655 0.0 19.857267 10-11 AGTACTT 8195 0.0 19.525118 12-13 >>END_MODULE