FastQCFastQC Report
Wed 25 May 2016
SRR1033116_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033116_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2176424
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA28100.034.8743061
TCAACGC28600.032.52584
CAACGCA30000.031.007935
ATCAACG30450.030.559523
GTACATG73300.029.8237721
GGTATCA19750.029.3417911
AACGCAG32400.028.700496
TACATGG74700.028.3847052
TATCAAC34650.027.8152
ACATGGG76100.026.3083483
CATGGGG50750.022.1253784
ACGCAGA41200.022.0003347
TATACCG1503.3124088E-521.9167396
CCGATCG1303.1771138E-421.6758949
TAACCGT1754.4704684E-621.4694587
TACGCGA805.459578E-520.5417580-81
TAGACCG1150.003968689620.4268325
ACCGTCC2301.01777914E-720.4193218
CGCAGAG43750.020.181298
ATACCGC2251.8603077E-618.7857747
Produced by FastQC (version 0.11.5)