##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1033116_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2176424 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.402416532807944 33.0 31.0 34.0 30.0 34.0 2 31.50787714158638 33.0 31.0 34.0 30.0 34.0 3 31.71828053724826 34.0 31.0 34.0 30.0 34.0 4 35.06805383509831 37.0 35.0 37.0 33.0 37.0 5 35.19175721274899 37.0 35.0 37.0 33.0 37.0 6 35.26088666546592 37.0 36.0 37.0 33.0 37.0 7 35.18627666300316 37.0 35.0 37.0 33.0 37.0 8 35.19829500134165 37.0 35.0 37.0 33.0 37.0 9 36.845269120355226 39.0 38.0 39.0 34.0 39.0 10-11 36.83495518336501 39.0 38.0 39.0 34.0 39.0 12-13 36.78131535950716 39.0 38.0 39.0 33.0 39.0 14-15 38.21235177520556 41.0 39.0 41.0 34.0 41.0 16-17 38.21292335500803 41.0 39.0 41.0 33.5 41.0 18-19 38.1945523942026 41.0 39.0 41.0 33.5 41.0 20-21 38.21305912818458 41.0 39.0 41.0 34.0 41.0 22-23 38.17338073831202 40.5 39.0 41.0 34.0 41.0 24-25 38.16143499612208 40.5 39.0 41.0 33.0 41.0 26-27 38.110373254476144 40.0 38.0 41.0 33.0 41.0 28-29 38.077213125751236 40.0 38.0 41.0 33.0 41.0 30-31 38.07139647421642 40.0 38.0 41.0 33.0 41.0 32-33 38.138338623356475 40.5 39.0 41.0 33.0 41.0 34-35 38.10558259787615 41.0 38.0 41.0 33.0 41.0 36-37 38.026093031504885 41.0 38.0 41.0 33.0 41.0 38-39 37.94017158421337 40.0 38.0 41.0 33.0 41.0 40-41 37.8727635332086 40.0 38.0 41.0 33.0 41.0 42-43 37.75365186195337 40.0 38.0 41.0 32.5 41.0 44-45 37.622411120259656 40.0 38.0 41.0 32.0 41.0 46-47 37.49181731133272 40.0 38.0 41.0 32.0 41.0 48-49 37.36138845188254 40.0 37.5 41.0 31.5 41.0 50-51 37.22989913730045 40.0 37.0 41.0 31.0 41.0 52-53 37.072920533866565 40.0 37.0 41.0 31.0 41.0 54-55 36.90856423196951 40.0 36.0 41.0 31.0 41.0 56-57 36.7384245900615 40.0 36.0 41.0 30.5 41.0 58-59 36.52742342484736 39.5 35.0 41.0 30.0 41.0 60-61 36.289609239743726 39.0 35.0 41.0 30.0 41.0 62-63 36.01294210135525 39.0 35.0 41.0 30.0 41.0 64-65 35.73032070037823 38.5 35.0 40.5 30.0 41.0 66-67 35.418487849793976 37.5 35.0 40.0 29.0 41.0 68-69 35.09465802619343 37.0 35.0 40.0 29.0 41.0 70-71 34.73579665543111 36.5 34.0 39.5 29.0 41.0 72-73 34.36080814216348 36.0 34.0 39.0 28.0 41.0 74-75 33.983667704454646 35.5 34.0 39.0 28.0 40.5 76-77 33.661128070633296 35.0 34.0 37.5 28.0 40.0 78-79 33.29903387391427 35.0 34.0 37.0 27.0 39.0 80-81 32.95021006936149 35.0 34.0 37.0 27.0 39.0 82-83 32.748483062123924 35.0 34.0 36.0 27.0 38.5 84-85 32.493836678882424 35.0 33.5 36.0 27.0 37.0 86-87 32.2528466420146 35.0 33.0 35.5 26.0 37.0 88-89 31.91163854101958 35.0 33.0 35.0 25.5 36.5 90-91 31.730954768004764 35.0 33.0 35.0 25.0 36.0 92-93 31.555221317169817 35.0 33.0 35.0 25.0 36.0 94-95 31.384286563647525 35.0 33.0 35.0 25.0 36.0 96-97 31.2065693541332 35.0 33.0 35.0 24.0 35.5 98-99 31.00731635931234 35.0 33.0 35.0 23.0 35.0 100 30.842158972700172 35.0 33.0 35.0 20.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.08471450493695798 1101 2 0.12369584727973404 1101 3 0.12169596954402095 1101 4 0.1101107137640156 1101 5 0.10591929599407734 1101 6 0.11197764080949213 1101 7 0.08053798197254736 1101 8 0.03660632598739255 1101 9 0.14637758483587504 1101 10-11 0.2065644815802088 1101 12-13 0.2327730709700262 1101 14-15 0.18133448727479617 1101 16-17 0.1002908525306907 1101 18-19 0.17300287891495003 1101 20-21 0.12990695637953564 1101 22-23 0.1788756625500767 1101 24-25 0.17935844355718444 1101 26-27 0.16351604390546015 1101 28-29 0.18155150400347964 1101 30-31 0.1666774928878496 1101 32-33 0.18626404112938388 1101 34-35 0.21598218151431325 1101 36-37 0.21394112790570574 1101 38-39 0.23367301497844295 1101 40-41 0.18481299078467828 1101 42-43 0.1008190127663866 1101 44-45 0.10263257393850012 1101 46-47 0.07232105655437948 1101 48-49 0.11588130302694566 1101 50-51 0.132709326116057 1101 52-53 0.10536078269733906 1101 54-55 0.045611178405039254 1101 56-57 0.0537893217942198 1101 58-59 0.12111606777094863 1101 60-61 0.054485041657805766 1101 62-63 0.05270051057111402 1101 64-65 -0.01655817488569511 1101 66-67 0.0761343568596402 1101 68-69 0.09162785121908712 1101 70-71 0.12266778275645152 1101 72-73 0.10826045388159855 1101 74-75 0.08014504483016793 1101 76-77 0.008151000663254138 1101 78-79 0.027278835895081954 1101 80-81 0.10116928820205828 1101 82-83 0.01422999582470652 1101 84-85 -0.06428070466690627 1101 86-87 0.09013890556742155 1101 88-89 0.27149948805170965 1101 90-91 0.3074973495694806 1101 92-93 0.32960076953038353 1101 94-95 0.34144703874726545 1101 96-97 0.3822157368204451 1101 98-99 0.4592131589541495 1101 100 0.42143893888071204 1102 1 0.10920682516320923 1102 2 0.051289992002548246 1102 3 0.1794000890088192 1102 4 0.18017471276260721 1102 5 -0.0021347334605223978 1102 6 0.09826267556711343 1102 7 0.12830957991074143 1102 8 0.14025924413169122 1102 9 0.25546092356168515 1102 10-11 0.1452954916568956 1102 12-13 0.16651002691753547 1102 14-15 0.14292193400859787 1102 16-17 0.21866046398579897 1102 18-19 0.16823493760775676 1102 20-21 0.1347337729465039 1102 22-23 0.21808976800149793 1102 24-25 0.2018552637627451 1102 26-27 0.17937709192752038 1102 28-29 0.23463068008931032 1102 30-31 0.20937404343411714 1102 32-33 0.2766958036425038 1102 34-35 0.2595776140671333 1102 36-37 0.1942720705145362 1102 38-39 0.12086610779662266 1102 40-41 0.12909726387677267 1102 42-43 0.16538209999406206 1102 44-45 0.09052766140803925 1102 46-47 0.20721170238946485 1102 48-49 0.190365561055458 1102 50-51 0.16490910737590525 1102 52-53 0.12493113120705601 1102 54-55 0.16301746331249944 1102 56-57 0.15980785848412182 1102 58-59 0.16089351928903284 1102 60-61 0.14935451361566976 1102 62-63 0.1770437331800636 1102 64-65 0.15297934138456526 1102 66-67 0.04205205242892873 1102 68-69 0.06283206774850214 1102 70-71 0.09367304334047333 1102 72-73 0.144978725685867 1102 74-75 0.054671682437557934 1102 76-77 0.012600644282294127 1102 78-79 -0.019188171151917288 1102 80-81 0.01678900069984479 1102 82-83 0.0348611067849518 1102 84-85 0.06285776676162413 1102 86-87 0.08878732054995453 1102 88-89 0.23109626147531515 1102 90-91 0.2938526633533911 1102 92-93 0.32154355762930464 1102 94-95 0.33366404765068935 1102 96-97 0.24891286657197043 1102 98-99 0.3034384507049275 1102 100 0.2627566414513858 1103 1 0.03500284740441728 1103 2 -0.004932848919285249 1103 3 0.05030609853367807 1103 4 0.028296770838778684 1103 5 0.07144890466129539 1103 6 0.053489672518509224 1103 7 0.10416071725816067 1103 8 0.06033731754455829 1103 9 -0.014806562543711266 1103 10-11 0.12086710716193494 1103 12-13 0.05871807111950744 1103 14-15 0.04554613877562019 1103 16-17 0.00808929026261751 1103 18-19 0.08098599219538727 1103 20-21 0.060395415600119406 1103 22-23 0.06263339298671866 1103 24-25 0.03163148433633722 1103 26-27 0.17140815688011202 1103 28-29 0.24663607659314124 1103 30-31 0.1678870364334628 1103 32-33 0.07897064198211723 1103 34-35 0.08983677089313602 1103 36-37 0.12614607842724723 1103 38-39 0.134906160908848 1103 40-41 0.11634944833946292 1103 42-43 -0.01913250269029021 1103 44-45 -0.10200922840253668 1103 46-47 0.05146494036351612 1103 48-49 0.04305708886835191 1103 50-51 -0.027447225879740245 1103 52-53 -0.07451257215087281 1103 54-55 -0.011621982211281079 1103 56-57 -0.22547215434088486 1103 58-59 -0.18661911556974786 1103 60-61 -0.1016196102673419 1103 62-63 -0.155718512531422 1103 64-65 -0.12035191731654038 1103 66-67 -0.07566139124942595 1103 68-69 -0.14239472419970411 1103 70-71 -0.12889430174323024 1103 72-73 -0.07860487143531003 1103 74-75 -0.09508509153392453 1103 76-77 -0.18284862327207918 1103 78-79 -0.23476255009989444 1103 80-81 -0.1759225518241223 1103 82-83 -0.13611682810726222 1103 84-85 -0.08941147499105284 1103 86-87 -0.02570511308999812 1103 88-89 0.12038946789527216 1103 90-91 0.11120738220895277 1103 92-93 0.1265313056139732 1103 94-95 0.19487381654444746 1103 96-97 0.16166561243587907 1103 98-99 0.18358262124461788 1103 100 0.1479101673114087 1104 1 -0.04004238106899649 1104 2 -0.11581409712593782 1104 3 -0.03239847625875214 1104 4 -0.10736055504424513 1104 5 -0.07541865825459126 1104 6 -0.090791146866124 1104 7 -0.16476542317372633 1104 8 -0.1511384209661486 1104 9 -0.10340427210721259 1104 10-11 -0.09921449220450285 1104 12-13 -0.028029242063098536 1104 14-15 -0.15562565123066463 1104 16-17 -0.03370474648460231 1104 18-19 -0.08086460457881373 1104 20-21 -0.11139454157170547 1104 22-23 -0.0986682341970706 1104 24-25 -0.06436186771412622 1104 26-27 -0.03293927756008941 1104 28-29 0.007370207629335823 1104 30-31 -0.06917263980758293 1104 32-33 -0.057327107493058804 1104 34-35 -0.044570650888893226 1104 36-37 -0.03877156729331688 1104 38-39 -0.14008973544891745 1104 40-41 -0.10712189212767953 1104 42-43 -0.16525350232673475 1104 44-45 -0.1328370855468748 1104 46-47 -0.17778479988648854 1104 48-49 -0.21291779949343237 1104 50-51 -0.13925907503741541 1104 52-53 -0.19374133102576963 1104 54-55 -0.2764257180925256 1104 56-57 -0.2618627920051395 1104 58-59 -0.27589865351780674 1104 60-61 -0.31808774512928295 1104 62-63 -0.31517946830334154 1104 64-65 -0.33288939651222904 1104 66-67 -0.3336443383688703 1104 68-69 -0.44878994242260717 1104 70-71 -0.3779965839577102 1104 72-73 -0.3809592176762351 1104 74-75 -0.31229718652702587 1104 76-77 -0.35432605530264993 1104 78-79 -0.3002837922318733 1104 80-81 -0.31006288076972055 1104 82-83 -0.29425361637828473 1104 84-85 -0.24776054263665515 1104 86-87 -0.24897262213545446 1104 88-89 -0.2146308528695684 1104 90-91 -0.16683400116887626 1104 92-93 -0.12575683716350738 1104 94-95 -0.16203318554131485 1104 96-97 -0.11864026436291297 1104 98-99 -0.17933200502040236 1104 100 -0.20693018062244306 1105 1 -0.013992668435840017 1105 2 0.05165242983101592 1105 3 0.0786181638716279 1105 4 -0.10239414099923749 1105 5 -0.12426070466591455 1105 6 -0.006873563552559858 1105 7 -0.05353826763717251 1105 8 0.0028801270195231155 1105 9 0.009381115803549278 1105 10-11 -0.04096828331217495 1105 12-13 0.02927154917625785 1105 14-15 0.014243309109431834 1105 16-17 0.05250449765193821 1105 18-19 0.020061463532520918 1105 20-21 0.05302959087234882 1105 22-23 0.12664272529773513 1105 24-25 0.12302161310201853 1105 26-27 0.0863660501246315 1105 28-29 0.17258396301404844 1105 30-31 0.1332729140528386 1105 32-33 0.14501974944251828 1105 34-35 0.10310671475897237 1105 36-37 0.09990940250380476 1105 38-39 0.030101178443722176 1105 40-41 0.0660315934759268 1105 42-43 0.04017064485529431 1105 44-45 0.03758103094877896 1105 46-47 0.05479364776682161 1105 48-49 0.08212667355009273 1105 50-51 0.08883089700321278 1105 52-53 0.06958073329933256 1105 54-55 0.07917060197647174 1105 56-57 0.15156336524721326 1105 58-59 0.08987728449519494 1105 60-61 0.10026755334096293 1105 62-63 0.24095160273419225 1105 64-65 0.16754104759064603 1105 66-67 0.2080034343657715 1105 68-69 0.11296799433624471 1105 70-71 0.11262369008314721 1105 72-73 0.06390124455436563 1105 74-75 0.14218955380734855 1105 76-77 0.054529283815895724 1105 78-79 0.05918243616212493 1105 80-81 0.033045473335448605 1105 82-83 0.12966370070080302 1105 84-85 0.05868784662096971 1105 86-87 0.1782719603494627 1105 88-89 0.16892170395604467 1105 90-91 0.22490925941429651 1105 92-93 0.21730711283385773 1105 94-95 0.2495286647693007 1105 96-97 0.2708889712731448 1105 98-99 0.2511960092649801 1105 100 0.35619396689237703 1106 1 0.19541621277830146 1106 2 0.154985221471577 1106 3 0.24519292691173078 1106 4 0.3184160687279345 1106 5 0.22662804744427234 1106 6 0.24885115462525675 1106 7 0.18157493310879858 1106 8 0.28550334409454337 1106 9 0.2626541294215272 1106 10-11 0.3086842583701639 1106 12-13 0.35826842469847975 1106 14-15 0.41853227289411876 1106 16-17 0.37191175378776364 1106 18-19 0.35202601748995477 1106 20-21 0.2504943766221075 1106 22-23 0.33106068255126786 1106 24-25 0.32548694438090564 1106 26-27 0.3684988434225005 1106 28-29 0.41875170148048113 1106 30-31 0.4225510800437675 1106 32-33 0.4315620753669336 1106 34-35 0.35232085358484966 1106 36-37 0.39864202555975936 1106 38-39 0.4175416248476722 1106 40-41 0.3442976210985762 1106 42-43 0.35818120832473 1106 44-45 0.36064360843348453 1106 46-47 0.3495537599662768 1106 48-49 0.3991562513565867 1106 50-51 0.3830824011213849 1106 52-53 0.34546470915241656 1106 54-55 0.2847832910299317 1106 56-57 0.3478769234183403 1106 58-59 0.357351226847733 1106 60-61 0.38617517799666956 1106 62-63 0.3715104409274943 1106 64-65 0.32262747367514066 1106 66-67 0.3408839248171631 1106 68-69 0.33182534790771 1106 70-71 0.3705585147749417 1106 72-73 0.38298261033831693 1106 74-75 0.4465020420968884 1106 76-77 0.4372762001952566 1106 78-79 0.41174179640608344 1106 80-81 0.35956518627203593 1106 82-83 0.33286317146848887 1106 84-85 0.28047761488848266 1106 86-87 0.25941690858864774 1106 88-89 0.39558523402206447 1106 90-91 0.3193423763927754 1106 92-93 0.32761236515143466 1106 94-95 0.38158271312481773 1106 96-97 0.3617430028714814 1106 98-99 0.3614299514886099 1106 100 0.4630588409396026 1107 1 0.1721283099463804 1107 2 0.2015855374216926 1107 3 0.19571909298412749 1107 4 0.21415780902982817 1107 5 0.10027358576708423 1107 6 0.16519262763978304 1107 7 0.14580941604015862 1107 8 0.16766611744237991 1107 9 0.2120051435569792 1107 10-11 0.15137576510870332 1107 12-13 0.10184842931428761 1107 14-15 0.14592770610895656 1107 16-17 0.13857449940498157 1107 18-19 0.2458572525400271 1107 20-21 0.2206469755175391 1107 22-23 0.1327518475770617 1107 24-25 0.2444989204288106 1107 26-27 0.21877726775101536 1107 28-29 0.18942447305713728 1107 30-31 0.1975673605988888 1107 32-33 0.16452465334810995 1107 34-35 0.21273414710537253 1107 36-37 0.1874226656539335 1107 38-39 0.24585203420523527 1107 40-41 0.2564576750569927 1107 42-43 0.21667272543989213 1107 44-45 0.2525425562802468 1107 46-47 0.22318744568715232 1107 48-49 0.14550377143444848 1107 50-51 0.20067685070443986 1107 52-53 0.25263065810303686 1107 54-55 0.20288534506348554 1107 56-57 0.1401372190803798 1107 58-59 0.14800471222832812 1107 60-61 0.09794754005474715 1107 62-63 0.24240939593054378 1107 64-65 0.17454191488533866 1107 66-67 0.17212326865712413 1107 68-69 0.1574260735553139 1107 70-71 0.19428755803291864 1107 72-73 0.2924799884533158 1107 74-75 0.29785534087728394 1107 76-77 0.17073546928779137 1107 78-79 0.18241255527674838 1107 80-81 0.2464696265698052 1107 82-83 0.18576567241223074 1107 84-85 0.20445332644714398 1107 86-87 0.11337455946555508 1107 88-89 0.23564434496062603 1107 90-91 0.24626339244254325 1107 92-93 0.2898127179564298 1107 94-95 0.19326638193896883 1107 96-97 0.22332939089614712 1107 98-99 0.20493275764802732 1107 100 0.2637620356854562 1108 1 -0.11988987718498834 1108 2 -0.10720802186458656 1108 3 -0.035193929368510624 1108 4 -0.034753349306114956 1108 5 -0.09890420649706755 1108 6 -0.17833172994017588 1108 7 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44-45 -0.15389257567559866 2214 46-47 -0.17568340307038 2214 48-49 -0.07298915392460259 2214 50-51 -0.03759001179697208 2214 52-53 -0.02404961066020661 2214 54-55 -0.058345024386298405 2214 56-57 -0.050802820928396386 2214 58-59 -0.07180046099902171 2214 60-61 -0.04222422518281377 2214 62-63 -0.038836990320263 2214 64-65 -0.10633312748275614 2214 66-67 -0.07754908474926481 2214 68-69 -0.10251946267523238 2214 70-71 -0.008941887626264133 2214 72-73 0.009593541591208066 2214 74-75 0.06959366223677677 2214 76-77 0.06264216643732823 2214 78-79 -0.012801013146891194 2214 80-81 0.06516874331965994 2214 82-83 0.02764877634864149 2214 84-85 0.026641746499379337 2214 86-87 0.04381645037851456 2214 88-89 -0.16971679734964695 2214 90-91 -0.35332140023625414 2214 92-93 -0.30319621423468135 2214 94-95 -0.2791524873147573 2214 96-97 -0.28383031854180274 2214 98-99 -0.28945155917891796 2214 100 -0.32307594207711077 2215 1 -0.11635802906391035 2215 2 -0.07325860546137264 2215 3 -0.10921843455819058 2215 4 -0.2628809705538657 2215 5 -0.17553097394466022 2215 6 -0.16389627248204874 2215 7 -0.04645410885809298 2215 8 -0.053699592695785725 2215 9 -0.2112366844752671 2215 10-11 -0.12389852397546264 2215 12-13 -0.1661389725054505 2215 14-15 -0.23484824199483256 2215 16-17 -0.143939206948545 2215 18-19 -0.13668059384512077 2215 20-21 -0.22718722620887633 2215 22-23 -0.18988178126914335 2215 24-25 -0.10010829371433516 2215 26-27 -0.2500095093441459 2215 28-29 -0.22041121271417552 2215 30-31 -0.23619109651286152 2215 32-33 -0.1919946133133763 2215 34-35 -0.2154690244735562 2215 36-37 -0.34809347738465846 2215 38-39 -0.26264590498133344 2215 40-41 -0.2678502026940066 2215 42-43 -0.3172679970113137 2215 44-45 -0.21379700485721997 2215 46-47 -0.2027940329140776 2215 48-49 -0.14867345852123037 2215 50-51 -0.19488397569470806 2215 52-53 -0.298148465467996 2215 54-55 -0.16741755562863858 2215 56-57 -0.23014705004371905 2215 58-59 -0.249990369037711 2215 60-61 -0.28314190443290244 2215 62-63 -0.11867492892623943 2215 64-65 -0.1680801461873216 2215 66-67 -0.22613654288861085 2215 68-69 -0.2606778160680463 2215 70-71 -0.24298108649620787 2215 72-73 -0.1923194062637421 2215 74-75 -0.12724510757646357 2215 76-77 -0.27784561529629315 2215 78-79 -0.2214553582468497 2215 80-81 -0.30540195494249645 2215 82-83 -0.2675476684385387 2215 84-85 -0.3334690430994769 2215 86-87 -0.40072824142616525 2215 88-89 -0.3664665306906656 2215 90-91 -0.28908571453830234 2215 92-93 -0.3466479645746112 2215 94-95 -0.3605394803624016 2215 96-97 -0.46396776803274875 2215 98-99 -0.509800762325014 2215 100 -0.4336126953719095 2216 1 -0.02865663228716997 2216 2 -0.032039835646713755 2216 3 -0.14032830642148753 2216 4 -0.18478497978664166 2216 5 -0.15980383578601476 2216 6 -0.12935347509678508 2216 7 -0.07188301784864848 2216 8 -0.13193699456332553 2216 9 -0.3109474759668771 2216 10-11 -0.16757816054893482 2216 12-13 -0.23559691182639853 2216 14-15 -0.2484041780678936 2216 16-17 -0.3351277273322637 2216 18-19 -0.276114504683143 2216 20-21 -0.35864480972126245 2216 22-23 -0.26378248102600566 2216 24-25 -0.28436553190992697 2216 26-27 -0.3404068403236664 2216 28-29 -0.2842299567555244 2216 30-31 -0.3223087459866818 2216 32-33 -0.2686923162953221 2216 34-35 -0.2592213623183497 2216 36-37 -0.2457684337068784 2216 38-39 -0.2517150550093845 2216 40-41 -0.3052186561583454 2216 42-43 -0.27321230954809295 2216 44-45 -0.20213586348717882 2216 46-47 -0.10494695989577707 2216 48-49 -0.02484352133512857 2216 50-51 -0.06794470491982452 2216 52-53 -0.0930791341144257 2216 54-55 -0.059975340309705416 2216 56-57 -0.06378102451672873 2216 58-59 -0.11880434615648028 2216 60-61 -0.12124016462020393 2216 62-63 0.037801015220935597 2216 64-65 0.020523345070273535 2216 66-67 0.018048068162435982 2216 68-69 -0.0312780776785786 2216 70-71 -0.11164068032702801 2216 72-73 -0.02676592435752667 2216 74-75 -0.028000744633466468 2216 76-77 -0.04319299622586925 2216 78-79 -0.1453713395258447 2216 80-81 -0.0878837425612673 2216 82-83 -0.03213624766752332 2216 84-85 -0.0057039143925692315 2216 86-87 -0.04103585048378022 2216 88-89 -0.015469496902539959 2216 90-91 0.03425390246570004 2216 92-93 0.11716975309584754 2216 94-95 0.13558953331797952 2216 96-97 0.10441789881182473 2216 98-99 0.1501391835738346 2216 100 0.15414144546442188 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 27113.0 3 4600.0 4 3170.0 5 2503.0 6 2211.0 7 2430.0 8 2730.0 9 3026.0 10 3152.0 11 3507.0 12 3910.0 13 4355.0 14 4804.0 15 5081.0 16 5128.0 17 5241.0 18 5046.0 19 4844.0 20 4903.0 21 5146.0 22 5750.0 23 8024.0 24 12049.0 25 16079.0 26 15999.0 27 14174.0 28 16181.0 29 20236.0 30 24440.0 31 30125.0 32 39355.0 33 53840.0 34 75818.0 35 117235.0 36 218635.0 37 455057.0 38 687234.0 39 262718.0 40 575.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.95159766074107 19.15823742390171 13.132285405816063 23.757879509541155 2 15.698037348569011 19.666167551044346 40.034363075564265 24.601432024822376 3 18.648233854200672 25.07859907437463 29.231591172627386 27.04157589879731 4 12.522092063864163 16.340979658064263 38.193656331372914 32.94327194669866 5 13.409010988505853 37.94300502653468 35.08548786345845 13.562496121501018 6 31.742386993902244 37.3823298303013 17.56668703847515 13.308596137321308 7 28.2101508810431 32.164000654326365 21.868377472793075 17.75747099183746 8 25.624528108532623 36.379862349000476 19.843958157846 18.151651384620898 9 25.188266213734025 17.25169700387079 20.552232241947646 37.00780454044754 10-11 24.405885924031157 26.917500279185706 28.20577572035657 20.47083807642657 12-13 24.941333580261155 24.081551435853875 28.292735381991747 22.684379601893227 14-15 22.181827456446378 25.930337640959465 25.32820747192874 26.559627430665415 16-17 22.25340332422257 29.316233129863427 25.84332013249096 22.58704341342304 18-19 22.24001474259286 28.33663754912666 27.589351693559948 21.833996014720533 20-21 23.285633662483583 27.548616530309673 27.194304349544513 21.971445457662234 22-23 22.830523016292073 27.32312419207119 27.2086068170925 22.63774597454424 24-25 22.67183331069882 27.50550868947077 27.252898309443 22.569759690387407 26-27 22.48109663527346 27.51234316704285 27.180817660281942 22.825742537401744 28-29 22.571855039468975 27.48731280557607 27.286325954854128 22.654506200100826 30-31 22.73940408315014 27.96288496531351 26.902448837690773 22.395262113845575 32-33 22.37816150458492 27.57754612209069 27.40979809341727 22.63449427990712 34-35 22.491499666144477 27.408228791902545 27.492898964243683 22.607372577709295 36-37 22.868393022403044 27.600883549564337 26.91696894893995 22.613754479092666 38-39 22.662839399771936 27.791600676335314 27.129051239591945 22.4165086843008 40-41 22.43898426699672 27.597971989524044 27.34004787656036 22.622995866918878 42-43 22.42732278628898 27.538386568268024 27.28244165018763 22.75184899525537 44-45 22.47449677644217 27.55271753702307 27.35862169898649 22.614163987548274 46-47 22.561877252734526 27.660845934555134 27.187346639439326 22.58993017327101 48-49 22.505521307202937 27.52853814532324 27.269157896405506 22.696782651068318 50-51 22.53836691726145 27.541447849986834 27.21150545722558 22.708679775526136 52-53 22.629318811907577 27.4399623026189 27.22020642119104 22.71051246428249 54-55 22.627927391658048 27.747228573929466 27.096325679350603 22.528518355061877 56-57 22.419661394346953 27.58149879627351 27.1612800509473 22.83755975843223 58-59 22.382208515383663 27.238811285045216 27.50083051250088 22.87814968707024 60-61 22.57086701457373 27.639680703564114 27.188328150720416 22.60112413114174 62-63 22.463230555713185 27.639498053061402 27.130989716571047 22.766281674654373 64-65 22.41760044330555 27.456846860631224 27.252585374684624 22.872967321378603 66-67 22.40869330484576 27.42124277041657 27.181786331707936 22.988277593029736 68-69 22.57912590187963 27.599486757115958 27.173706525697177 22.64768081530723 70-71 22.53951450214794 27.474327261566145 27.20410846520916 22.782049771076757 72-73 22.497563055577956 27.509983833346315 27.306803663951396 22.685649447124337 74-75 22.464614988050112 27.587110801626828 27.20434225540984 22.743931954913215 76-77 22.547527407707264 27.521682523877715 27.091949156144267 22.838840912270754 78-79 22.643574935896506 27.55865460893826 27.16905976414326 22.628710691021972 80-81 22.595068534441364 27.37568526400383 27.294815955698322 22.734430245856483 82-83 22.448244894789717 27.495319635201486 27.37422402664609 22.682211443362704 84-85 22.47275343799818 27.45750905576726 27.213502449370523 22.856235056864037 86-87 22.60534505982057 27.552428145131593 27.19483951242968 22.64738728261816 88-89 22.461302864052605 27.413131071742065 27.208684650987358 22.916881413217972 90-91 22.442288774325252 27.421384403730798 27.287993069475363 22.848333752468584 92-93 22.61334245543154 27.58959341174948 27.110082306904676 22.68698182591431 94-95 22.437346891610815 27.409660272706265 27.217887681996768 22.935105153686155 96-97 22.484069500034316 27.344671703323055 27.259371626620005 22.911887170022627 98-99 22.347264428909817 27.52253740828471 27.195563001906525 22.934635160898953 100 22.58481838300138 27.345389366804035 27.010247905770296 23.059544344424292 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 608.0 1 550.5 2 431.0 3 338.5 4 273.0 5 211.5 6 176.5 7 177.0 8 186.0 9 183.0 10 172.0 11 186.0 12 206.0 13 214.5 14 238.0 15 266.5 16 304.5 17 425.5 18 627.5 19 849.5 20 1174.5 21 1734.5 22 2593.5 23 3889.0 24 5732.0 25 7905.0 26 10612.5 27 14146.0 28 18625.5 29 23732.0 30 28634.0 31 33702.0 32 39164.5 33 44876.5 34 50476.5 35 55460.0 36 60143.0 37 64790.0 38 68626.5 39 71062.5 40 72855.5 41 74589.0 42 77056.5 43 79176.0 44 78862.5 45 78708.0 46 78133.0 47 75792.0 48 74669.0 49 74228.5 50 72604.5 51 70088.5 52 66203.5 53 63371.0 54 62075.0 55 59819.0 56 55353.5 57 50925.5 58 45988.5 59 39978.0 60 35146.5 61 31017.0 62 27602.0 63 23462.0 64 19252.0 65 15981.5 66 13003.0 67 10480.0 68 8619.5 69 7000.5 70 5601.0 71 4133.5 72 3081.5 73 2415.0 74 1816.0 75 1229.5 76 821.5 77 615.0 78 429.5 79 299.5 80 202.5 81 102.0 82 58.0 83 43.5 84 37.0 85 27.0 86 15.5 87 9.5 88 9.0 89 8.5 90 4.5 91 3.5 92 3.0 93 1.5 94 1.5 95 2.0 96 0.5 97 2.0 98 4.0 99 3.0 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.33035842280732064 2 0.1184971310737246 3 0.06717441086847048 4 0.03992788169952178 5 0.0445225746453816 6 0.009832642904140002 7 0.00643257012420374 8 0.015667902945381965 9 0.01759767398264309 10-11 0.020813959044744958 12-13 0.01605845184578005 14-15 0.018355798318709957 16-17 0.019596365414092105 18-19 0.019113922654776825 20-21 0.023800509459553837 22-23 0.015392221368630377 24-25 0.02497215616074809 26-27 0.019412577696257715 28-29 0.018884188007483836 30-31 0.019872046990843696 32-33 0.024880262301830894 34-35 0.015300327509713182 36-37 0.009281279750636823 38-39 0.01307190143097117 40-41 0.009304253215366123 42-43 0.008017739190525376 44-45 0.008247473837818367 46-47 0.008270447302547666 48-49 0.0037906216803343467 50-51 0.006202835476910749 52-53 0.006685278236226029 54-55 0.002871683091162384 56-57 0.0026419484438693934 58-59 0.002618974979140094 60-61 0.0035149401035827576 62-63 0.002687895373327991 64-65 0.0021595056845541125 66-67 0.0016770629252388322 68-69 0.0030554708089967765 70-71 0.0018149037136146265 72-73 0.0014013813484872433 74-75 0.0019986914314490192 76-77 0.0034230462446655616 78-79 0.0027797892322451874 80-81 0.0033541258504776643 82-83 0.0020446383609076173 84-85 0.00222842607874201 86-87 0.001860850643073225 88-89 0.5982749684804064 90-91 0.6083373460318394 92-93 0.6055345833348649 94-95 0.5938181163229224 96-97 0.5920491595387664 98-99 0.5880747501405976 100 0.5908775128375722 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2176424.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.41426917450852 #Duplication Level Percentage of deduplicated Percentage of total 1 79.68565861479524 52.92264780591005 2 12.576559844465221 16.705260615992565 3 3.685578435696569 7.3432499487634795 4 1.4437590510951168 3.8354480897025636 5 0.7781123586956705 2.5838881819212993 6 0.4475783715982072 1.7835354268806918 7 0.2818105087313751 1.310136728816351 8 0.20530518368518513 1.0908154985751841 9 0.14348843480549142 0.8576711579340739 >10 0.6991638753010623 8.115744650532474 >50 0.03841971132111118 1.7414302917905904 >100 0.014219312003196878 1.5550195683964687 >500 3.46297806573492E-4 0.15515203478411588 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 4.5946929458598144E-5 0.0 4 0.0 0.0 0.0 4.5946929458598144E-5 0.0 5 0.0 0.0 0.0 4.5946929458598144E-5 0.0 6 0.0 0.0 0.0 4.5946929458598144E-5 0.0 7 0.0 0.0 0.0 9.189385891719629E-5 0.0 8 0.0 0.0 0.0 9.189385891719629E-5 0.0 9 0.0 0.0 0.0 9.189385891719629E-5 0.0 10-11 0.0 0.0 0.0 1.1486732364649534E-4 0.0 12-13 0.0 0.0 0.0 1.378407883757944E-4 4.5946929458598144E-5 14-15 0.0 0.0 0.0 1.6081425310509348E-4 9.189385891719629E-5 16-17 0.0 0.0 0.0 2.297346472929907E-4 9.189385891719629E-5 18-19 0.0 0.0 0.0 2.297346472929907E-4 9.189385891719629E-5 20-21 0.0 0.0 0.0 2.297346472929907E-4 9.189385891719629E-5 22-23 0.0 0.0 0.0 2.986550414808879E-4 9.189385891719629E-5 24-25 0.0 0.0 0.0 3.2162850621018696E-4 9.189385891719629E-5 26-27 0.0 0.0 0.0 5.283896887738786E-4 9.189385891719629E-5 28-29 0.0 0.0 0.0 0.0016540894605095331 1.1486732364649534E-4 30-31 0.0 0.0 0.0 0.005329843817197384 1.378407883757944E-4 32-33 0.0 0.0 0.0 0.01380705230230874 1.378407883757944E-4 34-35 0.0 0.0 0.0 0.02568433356735636 1.378407883757944E-4 36-37 0.0 0.0 0.0 0.04528069898144847 1.378407883757944E-4 38-39 0.0 0.0 0.0 0.07838546165636842 1.378407883757944E-4 40-41 0.0 0.0 0.0 0.11741737823144754 1.8378771783439257E-4 42-43 0.0 0.0 0.0 0.15711552528367634 1.8378771783439257E-4 44-45 0.0 0.0 0.0 0.20460167687913752 1.8378771783439257E-4 46-47 0.0 0.0 0.0 0.2619204713787387 1.8378771783439257E-4 48-49 0.0 0.0 0.0 0.326544827662257 1.8378771783439257E-4 50-51 0.0 0.0 0.0 0.3951206198792147 1.8378771783439257E-4 52-53 0.0 0.0 0.0 0.46580997085126796 1.8378771783439257E-4 54-55 0.0 0.0 0.0 0.5419210594994357 1.8378771783439257E-4 56-57 0.0 0.0 0.0 0.6330567940805651 1.8378771783439257E-4 58-59 0.0 0.0 0.0 0.7369428015864556 1.8378771783439257E-4 60-61 0.0 0.0 0.0 0.8493749379716453 1.8378771783439257E-4 62-63 0.0 0.0 0.0 0.9617611274273763 1.8378771783439257E-4 64-65 0.0 0.0 0.0 1.0823028968620085 1.8378771783439257E-4 66-67 0.0 0.0 0.0 1.2166746920636786 1.8378771783439257E-4 68-69 0.0 0.0 0.0 1.3703671711026895 1.8378771783439257E-4 70-71 0.0 0.0 0.0 1.5446438745391524 1.8378771783439257E-4 72-73 0.0 0.0 0.0 1.7218152345315068 1.8378771783439257E-4 74-75 0.0 0.0 0.0 1.8991014618475077 1.8378771783439257E-4 76-77 0.0 0.0 0.0 2.090929892337155 1.8378771783439257E-4 78-79 0.0 0.0 0.0 2.313175190128394 1.8378771783439257E-4 80-81 0.0 0.0 0.0 2.5601629094330884 1.8378771783439257E-4 82-83 0.0 0.0 0.0 2.8139048273681966 1.8378771783439257E-4 84-85 0.0 0.0 0.0 3.0650966907183523 1.8378771783439257E-4 86-87 0.0 0.0 0.0 3.3258455153958972 1.8378771783439257E-4 88 0.0 0.0 0.0 3.538878453830687 1.8378771783439257E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 2810 0.0 34.874306 1 TCAACGC 2860 0.0 32.5258 4 CAACGCA 3000 0.0 31.00793 5 ATCAACG 3045 0.0 30.55952 3 GTACATG 7330 0.0 29.823772 1 GGTATCA 1975 0.0 29.341791 1 AACGCAG 3240 0.0 28.70049 6 TACATGG 7470 0.0 28.384705 2 TATCAAC 3465 0.0 27.815 2 ACATGGG 7610 0.0 26.308348 3 CATGGGG 5075 0.0 22.125378 4 ACGCAGA 4120 0.0 22.000334 7 TATACCG 150 3.3124088E-5 21.916739 6 CCGATCG 130 3.1771138E-4 21.675894 9 TAACCGT 175 4.4704684E-6 21.469458 7 TACGCGA 80 5.459578E-5 20.54175 80-81 TAGACCG 115 0.0039686896 20.426832 5 ACCGTCC 230 1.01777914E-7 20.419321 8 CGCAGAG 4375 0.0 20.18129 8 ATACCGC 225 1.8603077E-6 18.785774 7 >>END_MODULE