FastQCFastQC Report
Wed 25 May 2016
SRR1033116_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033116_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2176424
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44520.20455572994967894No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35780.16439811360286416No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31450.049.3632281
GTATCAA55550.043.021931
TCAACGC66450.035.6554834
ATCAACG67200.035.3274963
CAACGCA68450.034.4755365
AACGCAG70100.033.5854236
TATCAAC71550.033.1797032
ACGCAGA82200.028.4129057
CGCAGAG84450.027.544618
GTACATG75250.026.5700681
TACATGG74700.026.11672
ACATGGG73150.025.3847873
GCAGAGT96150.024.2905969
GTGGTAT14850.023.1263281
TAGACCG1452.5308811E-522.6939875
TGCACCG3550.022.511345
GAGTACT59150.021.72869512-13
GTACTTT64150.020.14499314-15
AGAGTAC89250.020.06078110-11
CCGGTCG2151.1967622E-619.6713949
Produced by FastQC (version 0.11.5)