FastQCFastQC Report
Wed 25 May 2016
SRR1033115_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033115_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2347635
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA27900.035.62781
TCAACGC27300.034.4162864
ATCAACG27600.034.221893
CAACGCA28800.032.6237755
GGTATCA16450.032.36111
AACGCAG30850.030.2920076
TATCAAC34050.028.1677592
GTACATG59550.024.6030751
ACGCAGA37300.024.550257
CGCAGAG37900.023.913788
TACATGG60300.023.4686682
CCGATCG1050.002347735722.3620079
ACATGGG62550.021.5611323
GTATAGG9000.018.3204561
GCAGAGT47350.018.2485339
TATACTG15250.017.5590765
TATACGG1650.001568536517.0964722
CATGGGG39400.016.8120084
GAGTACA47000.015.9371261
GTATTAC8800.015.5248011
Produced by FastQC (version 0.11.5)