##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033115_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2347635
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.74618158274178	33.0	31.0	34.0	30.0	34.0
2	31.89164456996083	34.0	31.0	34.0	30.0	34.0
3	31.973794478272815	34.0	31.0	34.0	30.0	34.0
4	35.32595228815382	37.0	35.0	37.0	33.0	37.0
5	35.423540286288116	37.0	35.0	37.0	35.0	37.0
6	35.46635188178742	37.0	37.0	37.0	35.0	37.0
7	35.432871805029315	37.0	37.0	37.0	35.0	37.0
8	35.4346029088849	37.0	37.0	37.0	35.0	37.0
9	37.102122348661524	39.0	38.0	39.0	35.0	39.0
10-11	37.10249272139834	39.0	38.0	39.0	35.0	39.0
12-13	37.06441567790564	39.0	38.0	39.0	35.0	39.0
14-15	38.54218841514971	41.0	39.0	41.0	35.0	41.0
16-17	38.55746975147329	41.0	39.0	41.0	35.0	41.0
18-19	38.54786987755763	41.0	39.0	41.0	35.0	41.0
20-21	38.58631069139794	41.0	39.0	41.0	35.0	41.0
22-23	38.5503608951136	41.0	39.0	41.0	34.0	41.0
24-25	38.55033405959615	41.0	39.0	41.0	34.0	41.0
26-27	38.51279010578732	41.0	39.0	41.0	34.0	41.0
28-29	38.48792444310977	40.5	39.0	41.0	34.0	41.0
30-31	38.486203136347854	41.0	39.0	41.0	34.0	41.0
32-33	38.54845472145372	41.0	39.0	41.0	34.0	41.0
34-35	38.51827733016419	41.0	39.0	41.0	34.0	41.0
36-37	38.43924225869865	41.0	39.0	41.0	34.0	41.0
38-39	38.36511318837894	41.0	39.0	41.0	34.0	41.0
40-41	38.30772394345799	41.0	39.0	41.0	34.0	41.0
42-43	38.19767276429258	41.0	38.5	41.0	33.0	41.0
44-45	38.07797847621117	40.0	38.0	41.0	33.0	41.0
46-47	37.96051579568375	40.0	38.0	41.0	33.0	41.0
48-49	37.8503144654088	40.0	38.0	41.0	33.0	41.0
50-51	37.74223612273629	40.0	38.0	41.0	33.0	41.0
52-53	37.603342725764435	40.0	38.0	41.0	32.5	41.0
54-55	37.46376864376276	40.0	37.0	41.0	32.0	41.0
56-57	37.32138556462142	40.0	37.0	41.0	32.0	41.0
58-59	37.136142969413896	40.0	36.5	41.0	31.5	41.0
60-61	36.931083409473786	40.0	36.0	41.0	31.0	41.0
62-63	36.680387070392115	39.0	35.5	41.0	31.0	41.0
64-65	36.42087292104607	39.0	35.0	41.0	31.0	41.0
66-67	36.14108517721026	39.0	35.0	41.0	30.5	41.0
68-69	35.83793093900883	38.0	35.0	40.0	30.0	41.0
70-71	35.48988556568632	37.0	35.0	40.0	30.0	41.0
72-73	35.12590330268547	37.0	35.0	39.5	29.5	41.0
74-75	34.75211670468365	36.0	34.5	39.0	29.0	41.0
76-77	34.409954912071086	36.0	34.0	39.0	29.0	40.5
78-79	34.033092239636915	35.5	34.0	37.5	29.0	39.5
80-81	33.659654290381596	35.0	34.0	37.0	29.0	39.0
82-83	33.41713511683034	35.0	34.0	37.0	29.0	39.0
84-85	33.13223052135447	35.0	34.0	36.0	29.0	38.0
86-87	32.862199830893644	35.0	34.0	36.0	28.5	37.0
88-89	32.4832867971384	35.0	34.0	36.0	27.0	37.0
90-91	32.282912590756226	35.0	34.0	35.0	27.0	36.5
92-93	32.09770364643567	35.0	34.0	35.0	27.0	36.0
94-95	31.923640387027795	35.0	34.0	35.0	27.0	36.0
96-97	31.744426412112617	35.0	33.5	35.0	26.0	36.0
98-99	31.551867730716232	35.0	33.0	35.0	25.5	35.5
100	31.3897352015965	35.0	33.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.24679203967635743
1101	2	0.2313294441378133
1101	3	0.1770315164162959
1101	4	0.22562452585430748
1101	5	0.14835489993429718
1101	6	0.18515442156807893
1101	7	0.18372772727995113
1101	8	0.17204370895959897
1101	9	0.1653665101775772
1101	10-11	0.17895309423930428
1101	12-13	0.22424093877714313
1101	14-15	0.2535193192511258
1101	16-17	0.13840721439392922
1101	18-19	0.21104791852523164
1101	20-21	0.15970621056053602
1101	22-23	0.21010172281291517
1101	24-25	0.15691501381134998
1101	26-27	0.2272587629975149
1101	28-29	0.27292523150527614
1101	30-31	0.3012236888342912
1101	32-33	0.26147073538880505
1101	34-35	0.2990389310535022
1101	36-37	0.30771240773345454
1101	38-39	0.28681788214654347
1101	40-41	0.23138714389873627
1101	42-43	0.25502292778146796
1101	44-45	0.24570291422408985
1101	46-47	0.24727124329285743
1101	48-49	0.21981887788784604
1101	50-51	0.2045044071963389
1101	52-53	0.23967828342114927
1101	54-55	0.21639558276624626
1101	56-57	0.23913007134085262
1101	58-59	0.22723956310390037
1101	60-61	0.316349712539278
1101	62-63	0.28472954790213834
1101	64-65	0.2656902896625084
1101	66-67	0.13122861563786614
1101	68-69	0.2286599774347593
1101	70-71	0.25929146922378266
1101	72-73	0.25111844677895334
1101	74-75	0.18508032368751515
1101	76-77	0.17186493709606765
1101	78-79	0.1926518860529569
1101	80-81	0.1653965627013747
1101	82-83	0.20441805408679414
1101	84-85	0.13288371996397075
1101	86-87	0.2267093680888408
1101	88-89	0.3669209397791562
1101	90-91	0.3743750236888346
1101	92-93	0.3515437496673499
1101	94-95	0.36313459524350833
1101	96-97	0.38543233379202135
1101	98-99	0.4652919121852719
1101	100	0.42809825039784855
1102	1	0.13805002189333493
1102	2	0.16947138134216289
1102	3	0.2015300083039655
1102	4	0.12926979831263452
1102	5	0.07635379942248477
1102	6	0.03531410913646482
1102	7	0.08023725272073534
1102	8	0.11302146221324705
1102	9	0.21320985862309527
1102	10-11	0.1447000964109293
1102	12-13	0.1570326267620672
1102	14-15	0.18919399082193422
1102	16-17	0.12475591102328565
1102	18-19	0.14965562314539937
1102	20-21	0.1288908231459871
1102	22-23	0.09923562885556692
1102	24-25	0.11291202506105691
1102	26-27	0.13431018483456825
1102	28-29	0.10933990504732805
1102	30-31	0.15440253103361812
1102	32-33	0.23673923971294641
1102	34-35	0.2501053496695107
1102	36-37	0.20365582667090365
1102	38-39	0.08590776003161693
1102	40-41	0.15447778568656645
1102	42-43	0.2050610268548212
1102	44-45	0.17773122781078143
1102	46-47	0.17717115781491088
1102	48-49	0.1085371620423814
1102	50-51	0.14323987724149845
1102	52-53	0.1364365191516228
1102	54-55	0.1559588382753816
1102	56-57	0.13478496136556828
1102	58-59	0.10619959481277874
1102	60-61	0.06386630811520888
1102	62-63	0.08056789178557011
1102	64-65	0.15162328755236132
1102	66-67	0.08938063574372279
1102	68-69	0.02500164984766684
1102	70-71	0.11592285982459316
1102	72-73	0.13116530114615443
1102	74-75	0.09334787063554018
1102	76-77	0.019724510789913552
1102	78-79	-0.004726914992453146
1102	80-81	-0.0390229298066771
1102	82-83	-0.004075378290046672
1102	84-85	-0.04034458186445988
1102	86-87	0.041324138040955916
1102	88-89	0.2236648696245993
1102	90-91	0.2406648560010396
1102	92-93	0.2784861347615646
1102	94-95	0.209016638744842
1102	96-97	0.12121746962012381
1102	98-99	0.11445732537385567
1102	100	0.09869679394885367
1103	1	-0.07380257087731579
1103	2	-0.056394714308289906
1103	3	-0.03119474804727318
1103	4	-0.05338421422946027
1103	5	-0.09407411933958798
1103	6	-0.040569814026952145
1103	7	-0.02456696976955186
1103	8	-0.049339415601799885
1103	9	-0.10897768681331144
1103	10-11	-0.1046051146295639
1103	12-13	-0.12225271646504865
1103	14-15	-0.1022158878121644
1103	16-17	-0.08720445052452419
1103	18-19	-0.1012208201342446
1103	20-21	-0.15245153378273812
1103	22-23	-0.09562178209271366
1103	24-25	-0.11079316961319563
1103	26-27	-0.0011071744139314887
1103	28-29	-0.03526917193318724
1103	30-31	-0.08879392560205446
1103	32-33	-0.005975864939671283
1103	34-35	-0.043320672727396925
1103	36-37	-0.048644534409291396
1103	38-39	-0.047216498829719455
1103	40-41	-0.0615643182960639
1103	42-43	-0.16393666324017886
1103	44-45	-0.1637995345126697
1103	46-47	-0.15355788517643276
1103	48-49	-0.18519020194794678
1103	50-51	-0.24320502989834836
1103	52-53	-0.34067928333122666
1103	54-55	-0.2541201726775739
1103	56-57	-0.2526170329778523
1103	58-59	-0.2906518490331109
1103	60-61	-0.32231557014479506
1103	62-63	-0.30680181758943803
1103	64-65	-0.3044965999151117
1103	66-67	-0.26282485228078656
1103	68-69	-0.33649693162296757
1103	70-71	-0.24246978895659765
1103	72-73	-0.1875215399573662
1103	74-75	-0.20437072828267588
1103	76-77	-0.20729192522721718
1103	78-79	-0.19183492889791864
1103	80-81	-0.2177730640609994
1103	82-83	-0.19862994070862072
1103	84-85	-0.2220378996888357
1103	86-87	-0.170308213707564
1103	88-89	-0.1251589620339928
1103	90-91	-0.12162473002803864
1103	92-93	-0.1251014512981925
1103	94-95	-0.22158364795663488
1103	96-97	-0.24525916172517626
1103	98-99	-0.3024288821223209
1103	100	-0.32216694905439525
1104	1	-0.030395238802448432
1104	2	-0.025896703009959054
1104	3	-0.09026110311585
1104	4	-0.06174059235443963
1104	5	-0.1461972586322915
1104	6	-0.2071145232446412
1104	7	-0.07829326033592565
1104	8	-0.1323809449865223
1104	9	-0.08929232658316977
1104	10-11	-0.12066179088564866
1104	12-13	-0.10531078933999538
1104	14-15	-0.15159350429574658
1104	16-17	-0.06575294210649929
1104	18-19	-0.09871604864338224
1104	20-21	-0.14618050613595557
1104	22-23	-0.09726280925190167
1104	24-25	-0.11553200885226289
1104	26-27	-0.07433362519095255
1104	28-29	-0.0321391435828744
1104	30-31	-0.036792598639998175
1104	32-33	-0.09578664223991495
1104	34-35	-0.08956469094961506
1104	36-37	-0.09376220216029196
1104	38-39	-0.2713575434031057
1104	40-41	-0.21087584788975278
1104	42-43	-0.16112487939915354
1104	44-45	-0.1659345154615366
1104	46-47	-0.24513846764659775
1104	48-49	-0.22314950147915624
1104	50-51	-0.25534995435555174
1104	52-53	-0.338509561052895
1104	54-55	-0.32263912977595055
1104	56-57	-0.3019794991072331
1104	58-59	-0.3571891572156929
1104	60-61	-0.31922272111198424
1104	62-63	-0.27933918846314754
1104	64-65	-0.385659398307439
1104	66-67	-0.3372460913359703
1104	68-69	-0.31921177061127537
1104	70-71	-0.2741216651625322
1104	72-73	-0.38139862008358705
1104	74-75	-0.4974932511126866
1104	76-77	-0.5515390571084353
1104	78-79	-0.4917524726597975
1104	80-81	-0.37831997443359455
1104	82-83	-0.3869783605440986
1104	84-85	-0.4574021351009563
1104	86-87	-0.42912267236973634
1104	88-89	-0.2573408394240886
1104	90-91	-0.28127393837849723
1104	92-93	-0.30840384540244514
1104	94-95	-0.23366231138193783
1104	96-97	-0.19548336955320522
1104	98-99	-0.15578747185072928
1104	100	-0.07330757916981767
1105	1	0.06322403586579384
1105	2	0.04037684706493749
1105	3	-0.008450938344441994
1105	4	-0.007868569737929931
1105	5	-0.009916324435366164
1105	6	-0.08321570681390966
1105	7	-0.041943548316687895
1105	8	-0.02998595775050461
1105	9	-0.06443779378712122
1105	10-11	-0.014444782087991825
1105	12-13	0.05421936952411954
1105	14-15	0.09990188590236215
1105	16-17	-0.008533591487285719
1105	18-19	-0.002040169405425729
1105	20-21	0.0505776533188822
1105	22-23	0.09496909437475409
1105	24-25	0.1065925085426116
1105	26-27	0.1088679191454176
1105	28-29	0.0833540102811341
1105	30-31	0.15385235167541111
1105	32-33	0.12617036107239699
1105	34-35	0.14969710782251866
1105	36-37	0.16488692545843975
1105	38-39	0.12767323783700846
1105	40-41	0.07686461076971796
1105	42-43	0.06270558115907932
1105	44-45	0.10905082178186376
1105	46-47	0.07257772889762748
1105	48-49	-0.0025366193508347123
1105	50-51	0.06431248578032012
1105	52-53	0.0811615290544978
1105	54-55	-4.888734414336682E-4
1105	56-57	-0.0019105103099974485
1105	58-59	-0.022669441609885155
1105	60-61	0.041010828119013354
1105	62-63	0.04220324914102491
1105	64-65	-0.021254875005254803
1105	66-67	0.03148088602642929
1105	68-69	0.032498181075474974
1105	70-71	0.07258797006183926
1105	72-73	-0.04184103448051246
1105	74-75	0.06511460981264605
1105	76-77	0.08200954096325574
1105	78-79	0.05232461757417184
1105	80-81	-0.0016185672309063648
1105	82-83	-0.013862472023241423
1105	84-85	0.022238574618661744
1105	86-87	-0.031206682580922518
1105	88-89	0.016446371276558125
1105	90-91	-0.016641562168025814
1105	92-93	-7.646547022162054E-4
1105	94-95	0.13109095345487276
1105	96-97	0.10582129932166495
1105	98-99	0.04837654004825964
1105	100	0.11231279256798388
1106	1	0.10599783951147401
1106	2	0.14541036987800027
1106	3	0.09329698152726351
1106	4	0.16017053953052596
1106	5	0.13098256847526102
1106	6	0.15592318740253575
1106	7	0.14035314184111058
1106	8	0.2440247418010273
1106	9	0.19184935573319706
1106	10-11	0.14590121904643638
1106	12-13	0.05465739006628212
1106	14-15	0.1584823794201924
1106	16-17	0.10070336154812765
1106	18-19	0.21923214683121017
1106	20-21	0.16737468421374047
1106	22-23	0.10429345564580927
1106	24-25	0.14049527402166717
1106	26-27	0.1598674417054795
1106	28-29	0.24267559668756178
1106	30-31	0.17528026436510658
1106	32-33	0.14398327806721056
1106	34-35	0.1319193663174758
1106	36-37	0.15026224630216234
1106	38-39	0.10571740715271716
1106	40-41	0.09194791791865953
1106	42-43	0.0826730989202531
1106	44-45	0.10274906341544465
1106	46-47	0.07286362156749249
1106	48-49	0.10887219574115647
1106	50-51	0.09706694698730445
1106	52-53	0.08958030509435133
1106	54-55	0.10249839074608502
1106	56-57	0.06809907929568482
1106	58-59	0.1225171884394527
1106	60-61	0.08628425430148923
1106	62-63	0.17496840173817674
1106	64-65	0.11057718855546739
1106	66-67	0.15425591387709403
1106	68-69	0.12032652305305191
1106	70-71	0.1570561958215606
1106	72-73	0.11035347536821405
1106	74-75	0.1476296981390277
1106	76-77	0.1349718721759885
1106	78-79	0.19908035195433627
1106	80-81	0.17556037481114117
1106	82-83	0.039597906582550024
1106	84-85	0.05296991467423595
1106	86-87	0.09335184869954816
1106	88-89	0.2349215831660345
1106	90-91	0.1911956756990847
1106	92-93	0.31474226817694984
1106	94-95	0.2730768179680325
1106	96-97	0.30390758128871553
1106	98-99	0.3100313387277467
1106	100	0.3881242976463959
1107	1	0.10700815701283162
1107	2	0.01977170628084224
1107	3	0.04204049335184479
1107	4	0.061392965126714216
1107	5	0.051808590317492076
1107	6	0.019893054868560966
1107	7	-0.0818537539995674
1107	8	-0.03075693667103252
1107	9	0.01482673290053782
1107	10-11	-0.011062793655057135
1107	12-13	-0.054925543683296496
1107	14-15	-0.10165738884803943
1107	16-17	-0.10760184666007433
1107	18-19	-0.10052670322986756
1107	20-21	-0.08961271380356095
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2216	7	0.04002929788054388
2216	8	0.0152829666892913
2216	9	-0.06021086557824873
2216	10-11	0.008839156726537567
2216	12-13	-0.07201070186809488
2216	14-15	-0.12174140749351636
2216	16-17	-0.10347898125577615
2216	18-19	-0.12016670832724685
2216	20-21	-0.12654495834322432
2216	22-23	-0.07122758499303217
2216	24-25	-0.08759882672568864
2216	26-27	-0.18635365120823622
2216	28-29	-0.17231909177260007
2216	30-31	-0.20521923999962155
2216	32-33	-0.1909030050912719
2216	34-35	-0.11644365451981287
2216	36-37	-0.16631284894765486
2216	38-39	-0.13611471077160076
2216	40-41	-0.15719102426336917
2216	42-43	-0.20900655964452852
2216	44-45	-0.10114994303648928
2216	46-47	-0.022067545322769888
2216	48-49	-0.03849205767704689
2216	50-51	0.04236140801920385
2216	52-53	0.007235576126589649
2216	54-55	0.01992204447815027
2216	56-57	0.026598437789644436
2216	58-59	-0.009456207226072877
2216	60-61	0.11174751601535604
2216	62-63	0.12600577881899966
2216	64-65	0.07661309066045163
2216	66-67	0.0363217666679958
2216	68-69	0.03516266516697897
2216	70-71	0.02245529608629937
2216	72-73	0.1089843778473707
2216	74-75	0.12117211562443941
2216	76-77	0.07174556858475256
2216	78-79	0.042535458461685494
2216	80-81	0.1455417695092649
2216	82-83	0.11549314100067676
2216	84-85	0.11641068626013151
2216	86-87	0.1269195468370654
2216	88-89	0.08840401739675485
2216	90-91	0.12670506836693818
2216	92-93	0.16615031427861737
2216	94-95	0.11279900193005687
2216	96-97	0.07147538408444731
2216	98-99	0.08602100955869219
2216	100	0.11992610535943271
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	21950.0
3	3731.0
4	2369.0
5	2015.0
6	1697.0
7	2068.0
8	2347.0
9	2575.0
10	2828.0
11	3175.0
12	3482.0
13	4052.0
14	4787.0
15	4841.0
16	4881.0
17	4880.0
18	4844.0
19	4535.0
20	4469.0
21	4635.0
22	5399.0
23	7419.0
24	11381.0
25	15955.0
26	16496.0
27	13816.0
28	15119.0
29	19115.0
30	23157.0
31	28192.0
32	37618.0
33	51396.0
34	71950.0
35	109044.0
36	203162.0
37	446636.0
38	793661.0
39	387015.0
40	943.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.16519029565457	20.4608055442657	13.750443937006459	23.62356022307327
2	14.97883403374784	20.485183823623487	41.20057898278831	23.33540315984036
3	17.788991674257286	25.976675303706536	30.324918364415453	25.909414657620722
4	11.926641471227299	17.193437140588934	40.1670671545531	30.712854233630672
5	12.436637886399694	38.30431797756017	36.45830980669735	12.800734329342788
6	29.865225872440636	39.180475153669434	18.269973404719842	12.684325569170094
7	26.322726416870644	32.95256749630026	23.34099550919248	17.383710577636613
8	24.2490276948796	37.06828864500138	21.070849932495676	17.61183372762335
9	24.315353298148086	17.53534856058037	21.82945097043451	36.319847170837036
10-11	23.131542962779445	27.44789861492576	29.628838277207	19.791720145087798
12-13	23.804632572002372	24.794024835030285	29.54345704269125	21.85788555027609
14-15	21.225515079875485	26.950416552256545	26.51632200195596	25.30774636591201
16-17	20.93607633739342	30.254064594686596	27.068703057611348	21.741156010308632
18-19	20.82835925817048	29.27732119957225	28.96628706058786	20.928032481669415
20-21	22.07465188274322	28.538648876421252	28.414322806377694	20.972376434457836
22-23	21.59529306811363	28.319085879438653	28.49233031769149	21.593290734756227
24-25	21.57708993207609	28.496172622684423	28.334841617391792	21.591895827847697
26-27	21.29080071285816	28.42076695732863	28.496326002664485	21.792106327148726
28-29	21.256048732397005	28.432684925694	28.612943328734918	21.698323013174072
30-31	21.554804341495597	28.901736074201906	28.182165409439946	21.361294174862554
32-33	21.167967025756653	28.563972383985988	28.728562395932926	21.539498194324437
34-35	21.33926365208797	28.333035113281017	28.73258607908796	21.59511515554306
36-37	21.714112026827955	28.65949249425535	28.172102605348382	21.454292873568313
38-39	21.47111261638347	28.740640862606266	28.371302408506775	21.41694411250349
40-41	21.230270936679343	28.486830179942135	28.638040447841405	21.644858435537117
42-43	21.252405826637347	28.320296358649856	28.650849909645714	21.77644790506708
44-45	21.367484775348665	28.520296588625882	28.580767065550294	21.531451570475156
46-47	21.404329152598116	28.680167365727854	28.4286799969669	21.48682348470713
48-49	21.35251433260853	28.574227864461033	28.35269729074988	21.720560512180562
50-51	21.373888087910572	28.383926314065338	28.45385246668071	21.788333131343382
52-53	21.401508616374628	28.369221226957432	28.483387711511515	21.745882445156422
54-55	21.561837741820746	28.76724932962167	28.224897819220423	21.44601510933716
56-57	21.4055825185336	28.503312268203523	28.271028584779817	21.820076628483058
58-59	21.243765327103876	28.177271095014945	28.662541527213182	21.91642205066799
60-61	21.5370299255753	28.56970944697227	28.299639558934125	21.593621068518306
62-63	21.357716886341002	28.575767411864035	28.30433734344423	21.762178358350734
64-65	21.385638204408153	28.28703570588041	28.44343063555807	21.883895454153368
66-67	21.237343118134305	28.405108821336295	28.326091498651913	22.031456561877487
68-69	21.679180326192892	28.539748369376305	28.196073553566926	21.584997750863874
70-71	21.55628183461071	28.37286868512608	28.296279257491484	21.774570222771725
72-73	21.359314905049313	28.450830576247014	28.41257853276577	21.7772759859379
74-75	21.497685065874137	28.49899236789498	28.249544318560833	21.75377824767005
76-77	21.450536624431056	28.573275485666944	28.119652658415497	21.85653523148651
78-79	21.627401650740858	28.60999458158967	28.225338565450308	21.537265202219164
80-81	21.535623879815	28.372695334173216	28.336700322357693	21.75498046365409
82-83	21.395239174365386	28.422083033733518	28.47083544899079	21.711842342910302
84-85	21.427222007460912	28.332135284714923	28.361399618967294	21.87924308885687
86-87	21.60952521116954	28.46803336456407	28.174497104572133	21.747944319694255
88-89	21.404783964769944	28.35591494526159	28.28601879953477	21.953282290433698
90-91	21.354150036184357	28.249343549101543	28.406146004794724	21.99036040991938
92-93	21.529357435441927	28.45460282629403	28.229317898612138	21.786721839651904
94-95	21.314814362486914	28.252614943304504	28.36617305313191	22.06639764107667
96-97	21.34427019100649	28.22717148075237	28.367017506571283	22.061540821669855
98-99	21.383399455071466	28.30296775443121	28.294997580023658	22.018635210473665
100	21.520578797976057	28.34135505368382	28.128299532409123	22.009766615931
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	285.0
1	259.0
2	223.5
3	198.0
4	174.0
5	153.5
6	136.5
7	134.5
8	128.0
9	144.0
10	148.5
11	145.0
12	161.0
13	173.5
14	217.5
15	260.5
16	346.0
17	506.0
18	723.5
19	1054.5
20	1582.0
21	2492.5
22	3839.5
23	5833.0
24	8434.0
25	11453.5
26	15750.5
27	21455.5
28	28432.0
29	35903.5
30	42875.5
31	50450.0
32	58288.0
33	65583.0
34	72423.0
35	77936.0
36	82603.0
37	86796.5
38	89358.0
39	90075.5
40	90315.0
41	89920.5
42	87458.0
43	85116.0
44	83351.0
45	80779.0
46	78331.5
47	75071.5
48	72131.5
49	70190.0
50	67964.0
51	64949.0
52	60416.0
53	56565.5
54	53707.5
55	50927.5
56	46862.5
57	41988.5
58	37916.5
59	34076.0
60	30453.5
61	26285.0
62	22102.0
63	18276.0
64	14849.5
65	11981.5
66	9328.0
67	7075.0
68	5469.5
69	4260.5
70	3168.0
71	2352.0
72	1793.5
73	1350.0
74	1036.5
75	816.5
76	582.5
77	417.0
78	302.5
79	228.0
80	159.0
81	84.5
82	56.5
83	40.0
84	31.5
85	26.0
86	16.5
87	9.5
88	7.0
89	7.5
90	5.0
91	3.5
92	5.5
93	4.0
94	3.0
95	2.5
96	0.5
97	1.5
98	3.0
99	4.0
100	4.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3312269581940975
2	0.12067463638938762
3	0.06551273941647659
4	0.038932798326826785
5	0.04276644367629551
6	0.009626709433110343
7	0.007369118282867652
8	0.016101310467768627
9	0.01869967009351965
10-11	0.021489711986744105
12-13	0.016442078943276955
14-15	0.019189524777062872
16-17	0.02027572429274568
18-19	0.01980716763892172
20-21	0.02474831053379252
22-23	0.01590962820029519
24-25	0.02542984748480918
26-27	0.019764571579483182
28-29	0.019125630687905064
30-31	0.020084042025272242
32-33	0.02517427112817793
34-35	0.015973522289453004
36-37	0.009924881849180132
38-39	0.014056699614718643
40-41	0.010286948354407733
42-43	0.008902576422655139
44-45	0.00888127839293587
46-47	0.00856180794714681
48-49	0.004408692151889029
50-51	0.006751475421008802
52-53	0.00792286705556869
54-55	0.0034076847550833073
56-57	0.0031734064281713297
58-59	0.0033224926362062247
60-61	0.004025327616942157
62-63	0.0029391281012593525
64-65	0.00223629312052342
66-67	0.002065908882769255
68-69	0.00285393598238227
70-71	0.00223629312052342
72-73	0.0020446108530499845
74-75	0.0022149950908041498
76-77	0.0038336453494687206
78-79	0.0035354729333989315
80-81	0.003727155200872367
82-83	0.002513167506873939
84-85	0.0028752340121015406
86-87	0.002321485239400503
88-89	0.6037139504224464
90-91	0.6152787805600104
92-93	0.6119988839832428
94-95	0.5975375218038579
96-97	0.5962170439612631
98-99	0.5935547902463544
100	0.5951095464158611
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2347635.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	69.39236243569262
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.70123433725584	54.612645772672344
2	13.503949714042001	18.741419457403406
3	4.0417142366291525	8.413922975090065
4	1.5688771582116747	4.354723695188159
5	0.757839104220881	2.6294122894018
6	0.41334762608596903	1.720990096877443
7	0.2410885986257402	1.171079519046541
8	0.16276616025963042	0.9035782708001842
9	0.1210170265747063	0.7557891632065679
>10	0.46657631724879595	5.501640664773785
>50	0.017948761750372084	0.83389622973959
>100	0.003640959095238637	0.36090186580017447
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	4.259605943854134E-5	0.0
4	0.0	0.0	0.0	4.259605943854134E-5	0.0
5	0.0	0.0	0.0	4.259605943854134E-5	0.0
6	0.0	0.0	0.0	4.259605943854134E-5	0.0
7	0.0	0.0	0.0	4.259605943854134E-5	0.0
8	0.0	0.0	0.0	4.259605943854134E-5	0.0
9	0.0	0.0	0.0	8.519211887708268E-5	0.0
10-11	0.0	0.0	0.0	8.519211887708268E-5	0.0
12-13	0.0	0.0	0.0	8.519211887708268E-5	0.0
14-15	0.0	0.0	0.0	1.0649014859635335E-4	0.0
16-17	0.0	0.0	0.0	1.7038423775416535E-4	0.0
18-19	0.0	0.0	0.0	1.7038423775416535E-4	0.0
20-21	0.0	0.0	0.0	1.7038423775416535E-4	0.0
22-23	0.0	0.0	0.0	1.7038423775416535E-4	0.0
24-25	0.0	0.0	0.0	2.342783269119774E-4	0.0
26-27	0.0	0.0	0.0	3.8336453494687203E-4	0.0
28-29	0.0	0.0	0.0	0.0012778817831562402	0.0
30-31	0.0	0.0	0.0	0.005984746351115058	0.0
32-33	0.0	0.0	0.0	0.013737229168929582	0.0
34-35	0.0	0.0	0.0	0.02670772926796542	0.0
36-37	0.0	0.0	0.0	0.0473455200659387	0.0
38-39	0.0	0.0	0.0	0.08453187995578529	0.0
40-41	0.0	0.0	0.0	0.12712793939432662	0.0
42-43	0.0	0.0	0.0	0.1678923682770107	0.0
44-45	0.0	0.0	0.0	0.21847518886027856	0.0
46-47	0.0	0.0	0.0	0.278940295233288	0.0
48-49	0.0	0.0	0.0	0.34854225635586455	0.0
50-51	0.0	0.0	0.0	0.4223825253925759	0.0
52-53	0.0	0.0	0.0	0.4981396171040217	0.0
54-55	0.0	0.0	0.0	0.5762607901143064	0.0
56-57	0.0	0.0	0.0	0.6726769706534448	0.0
58-59	0.0	0.0	0.0	0.7825109099157237	0.0
60-61	0.0	0.0	0.0	0.9044634280882675	0.0
62-63	0.0	0.0	0.0	1.0233703280109556	0.0
64-65	0.0	0.0	0.0	1.149454663949038	0.0
66-67	0.0	0.0	0.0	1.2935571330296236	0.0
68-69	0.0	0.0	0.0	1.4579140283732352	0.0
70-71	0.0	0.0	0.0	1.636753583925951	0.0
72-73	0.0	0.0	0.0	1.8252411469414964	0.0
74-75	0.0	0.0	0.0	2.0163909636719506	0.0
76-77	0.0	0.0	0.0	2.2201066179367746	0.0
78-79	0.0	0.0	0.0	2.4552155680078034	0.0
80-81	0.0	0.0	0.0	2.712708747313786	0.0
82-83	0.0	0.0	0.0	2.9796369537854055	0.0
84-85	0.0	0.0	0.0	3.248418088842601	0.0
86-87	0.0	0.0	0.0	3.526655549095153	0.0
88	0.0	0.0	0.0	3.7605931075316223	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	2790	0.0	35.6278	1
TCAACGC	2730	0.0	34.416286	4
ATCAACG	2760	0.0	34.22189	3
CAACGCA	2880	0.0	32.623775	5
GGTATCA	1645	0.0	32.3611	1
AACGCAG	3085	0.0	30.292007	6
TATCAAC	3405	0.0	28.167759	2
GTACATG	5955	0.0	24.603075	1
ACGCAGA	3730	0.0	24.55025	7
CGCAGAG	3790	0.0	23.91378	8
TACATGG	6030	0.0	23.468668	2
CCGATCG	105	0.0023477357	22.362007	9
ACATGGG	6255	0.0	21.561132	3
GTATAGG	900	0.0	18.320456	1
GCAGAGT	4735	0.0	18.248533	9
TATACTG	1525	0.0	17.559076	5
TATACGG	165	0.0015685365	17.096472	2
CATGGGG	3940	0.0	16.812008	4
GAGTACA	4700	0.0	15.937126	1
GTATTAC	880	0.0	15.524801	1
>>END_MODULE