FastQCFastQC Report
Wed 25 May 2016
SRR1033115_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033115_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2347635
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41020.17472903581689658No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32850.1399280552556083No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29150.050.8494261
GTATCAA57350.045.0456661
TCAACGC67250.037.680944
ATCAACG68400.037.0474133
CAACGCA68950.036.7526745
AACGCAG72150.035.2379266
TATCAAC72400.035.1954042
ACGCAGA83400.030.5409587
CGCAGAG84050.030.136398
TAGACCG650.00614600428.9321775
GCAGAGT95450.026.1924349
GTACATG53100.024.901521
TGGTATC14950.023.8999842
GTGGTAT17550.023.3268551
TACATGG56450.022.4866662
AGAGTAC88600.021.69350810-11
ACATGGG56500.021.6346633
GAGTACT69600.021.43706912-13
GTAACGG1354.0707318E-420.8950163
GGACCGT1805.6743374E-620.8865596
Produced by FastQC (version 0.11.5)