##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1033115_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2347635 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.77055930755846 34.0 31.0 34.0 31.0 34.0 2 32.866854941249386 34.0 31.0 34.0 31.0 34.0 3 32.95434256176961 34.0 31.0 34.0 31.0 34.0 4 36.37787347692465 37.0 37.0 37.0 35.0 37.0 5 36.31414295663508 37.0 37.0 37.0 35.0 37.0 6 36.39944369546373 37.0 37.0 37.0 35.0 37.0 7 36.37195092082032 37.0 37.0 37.0 35.0 37.0 8 36.370140162333584 37.0 37.0 37.0 35.0 37.0 9 38.19439734030205 39.0 39.0 39.0 37.0 39.0 10-11 38.17453543672674 39.0 39.0 39.0 37.0 39.0 12-13 38.14002112764548 39.0 39.0 39.0 37.0 39.0 14-15 39.681088414510775 41.0 40.0 41.0 37.0 41.0 16-17 39.69484395998526 41.0 40.0 41.0 37.0 41.0 18-19 39.713920392224516 41.0 40.0 41.0 37.0 41.0 20-21 39.70921416659745 41.0 40.0 41.0 37.0 41.0 22-23 39.638785415961166 41.0 40.0 41.0 37.0 41.0 24-25 39.59194465919958 41.0 40.0 41.0 37.0 41.0 26-27 39.49296675164581 41.0 39.5 41.0 36.5 41.0 28-29 39.40490280644138 41.0 39.0 41.0 36.0 41.0 30-31 39.31119935594758 41.0 39.0 41.0 36.0 41.0 32-33 39.3392005145604 41.0 39.0 41.0 36.0 41.0 34-35 39.283488489479836 41.0 39.0 41.0 35.5 41.0 36-37 39.194737043876074 41.0 39.0 41.0 35.0 41.0 38-39 39.10305797110709 41.0 39.0 41.0 35.0 41.0 40-41 38.99010046280618 41.0 39.0 41.0 35.0 41.0 42-43 38.88004843171959 41.0 39.0 41.0 35.0 41.0 44-45 38.73902012024868 41.0 39.0 41.0 35.0 41.0 46-47 38.536049471063436 41.0 39.0 41.0 35.0 41.0 48-49 38.36117411778237 41.0 39.0 41.0 35.0 41.0 50-51 38.23934747096546 41.0 38.0 41.0 34.5 41.0 52-53 38.10937454076124 40.5 38.0 41.0 34.0 41.0 54-55 37.98544599139134 40.0 38.0 41.0 34.0 41.0 56-57 37.84063770560586 40.0 37.5 41.0 34.0 41.0 58-59 37.6650633509894 40.0 37.0 41.0 34.0 41.0 60-61 37.485968432060346 40.0 37.0 41.0 33.0 41.0 62-63 37.26578620611807 40.0 36.0 41.0 33.0 41.0 64-65 37.026571847838355 39.0 36.0 41.0 33.0 41.0 66-67 36.75821113588782 39.0 35.0 41.0 32.5 41.0 68-69 36.446426510083555 38.5 35.0 41.0 32.0 41.0 70-71 36.11804518164024 37.5 35.0 40.0 32.0 41.0 72-73 35.76663578452357 37.0 35.0 40.0 31.5 41.0 74-75 35.42813704004243 36.5 35.0 39.0 31.0 41.0 76-77 35.08221337644055 36.0 35.0 39.0 31.0 41.0 78-79 34.73187633512023 36.0 35.0 38.5 31.0 40.0 80-81 34.380627525147645 35.0 35.0 37.0 31.0 39.0 82-83 34.04241417426474 35.0 34.5 37.0 31.0 39.0 84-85 33.7568393297936 35.0 34.0 36.5 30.0 38.0 86-87 33.50602478664699 35.0 34.0 36.0 30.0 37.0 88-89 33.27535668875272 35.0 34.0 36.0 30.0 37.0 90-91 33.06616871873183 35.0 34.0 35.5 30.0 36.5 92-93 32.89381845985427 35.0 34.0 35.0 30.0 36.0 94-95 32.73974510518032 35.0 34.0 35.0 29.5 36.0 96-97 32.61265209455473 35.0 34.0 35.0 29.5 36.0 98-99 32.50177881144215 35.0 34.0 35.0 29.0 36.0 100 32.40390520672933 35.0 34.0 35.0 29.0 36.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.11519870152586975 1101 2 0.12852133725908033 1101 3 0.08909106670836309 1101 4 0.08257102608144606 1101 5 0.05934456079683059 1101 6 0.1063182152043467 1101 7 0.10912579753274088 1101 8 0.12951652971621286 1101 9 0.18001389780965127 1101 10-11 0.16077764143056328 1101 12-13 0.15481589566953602 1101 14-15 0.16101816066957042 1101 16-17 0.17974316264967882 1101 18-19 0.26459079389782403 1101 20-21 0.27527621723483975 1101 22-23 0.23712657025694028 1101 24-25 0.2181453639005113 1101 26-27 0.1856587539130885 1101 28-29 0.17830894130707264 1101 30-31 0.1874333131847834 1101 32-33 0.16094135983583158 1101 34-35 0.14118744222775348 1101 36-37 0.13125108011774955 1101 38-39 0.031059353584403482 1101 40-41 0.04992841755601063 1101 42-43 -0.0013112491219189337 1101 44-45 0.07588918664368549 1101 46-47 0.14065412802816013 1101 48-49 -0.05968436296121382 1101 50-51 -0.07049741297716139 1101 52-53 -0.0055806224053256415 1101 54-55 0.054702762367575986 1101 56-57 0.01104524396508566 1101 58-59 0.0431874391999969 1101 60-61 0.059990798026156256 1101 62-63 0.04800574441967598 1101 64-65 0.10429485197045096 1101 66-67 0.06694318461877913 1101 68-69 0.10132335207097043 1101 70-71 0.09312482241843867 1101 72-73 0.11925795704181041 1101 74-75 0.0456998820389245 1101 76-77 0.05639063416088419 1101 78-79 0.10427298829274179 1101 80-81 -0.023617453378030007 1101 82-83 -0.04561416130645313 1101 84-85 -0.0180326859124591 1101 86-87 -0.013498150643975748 1101 88-89 0.02597644182851866 1101 90-91 0.07197590964406686 1101 92-93 0.11911058566253985 1101 94-95 0.07677988771014554 1101 96-97 0.06304000108288932 1101 98-99 0.1450685054983154 1101 100 0.1345317396725889 1102 1 0.15124065546461196 1102 2 0.1844382117286827 1102 3 0.1036660276438397 1102 4 0.08382014925518888 1102 5 0.08707350058780605 1102 6 0.10838513316222986 1102 7 0.1084415320273493 1102 8 0.1304471999697867 1102 9 0.1701500227438757 1102 10-11 0.16536288315986525 1102 12-13 0.18341402460732326 1102 14-15 0.25868781538207486 1102 16-17 0.2125804138985572 1102 18-19 0.2618168912443224 1102 20-21 0.24666492749762625 1102 22-23 0.27916350849014293 1102 24-25 0.2677865063654963 1102 26-27 0.2586078642718661 1102 28-29 0.32496976790903176 1102 30-31 0.256482744186755 1102 32-33 0.2739160636265865 1102 34-35 0.22318791640078928 1102 36-37 0.19861602485872254 1102 38-39 0.20867159454961381 1102 40-41 0.1470565101757586 1102 42-43 0.16667679702964477 1102 44-45 0.20086514657030108 1102 46-47 0.18840222879030932 1102 48-49 0.22384714447291287 1102 50-51 0.1973603508666102 1102 52-53 0.25978792512849935 1102 54-55 0.21759791537003537 1102 56-57 0.18770793805565944 1102 58-59 0.07973267363610148 1102 60-61 0.16769416936185877 1102 62-63 0.16935724900644544 1102 64-65 0.12411266231699614 1102 66-67 0.13576138902079293 1102 68-69 0.17254969457337666 1102 70-71 0.19534686489381414 1102 72-73 0.15431674162564946 1102 74-75 0.13658476039567802 1102 76-77 0.11597562516254811 1102 78-79 0.13507074792121188 1102 80-81 0.14495509557677622 1102 82-83 0.137106727341461 1102 84-85 0.2278132086193878 1102 86-87 0.15777101729185716 1102 88-89 0.18912818238747064 1102 90-91 0.14671791474661688 1102 92-93 0.07709454139921945 1102 94-95 0.15051883816232703 1102 96-97 0.11163005678285742 1102 98-99 0.15446565250598354 1102 100 0.14589575374331076 1103 1 0.004902620246504341 1103 2 0.1054436067432647 1103 3 0.11763468775281183 1103 4 0.028915427813736017 1103 5 -0.024939905117435046 1103 6 0.020319411012785338 1103 7 0.032502628521690724 1103 8 0.06312486684517182 1103 9 0.08274671325605709 1103 10-11 0.01695098395253325 1103 12-13 -0.00845887618475416 1103 14-15 0.07307153141024969 1103 16-17 0.07136810831161 1103 18-19 0.12508160204772167 1103 20-21 0.03238671527569892 1103 22-23 0.06422923100490863 1103 24-25 0.08144543170255503 1103 26-27 0.11480070572171286 1103 28-29 0.08672889409786677 1103 30-31 0.12648834633657202 1103 32-33 0.11182122856395438 1103 34-35 0.07822612499876413 1103 36-37 0.11020407268028976 1103 38-39 0.11844414945871762 1103 40-41 0.12490920450478171 1103 42-43 0.036747654087328385 1103 44-45 0.09902273886238078 1103 46-47 0.07500641452961787 1103 48-49 0.0264735609016995 1103 50-51 -0.023686927843911576 1103 52-53 -0.03230039448957456 1103 54-55 0.05600539240433022 1103 56-57 0.03591223623980255 1103 58-59 0.06854143410519953 1103 60-61 -0.061465358384403146 1103 62-63 -0.04048751098334691 1103 64-65 0.026103946197494565 1103 66-67 0.0197877547434544 1103 68-69 0.03135168204882177 1103 70-71 -1.2217952956916633E-4 1103 72-73 0.0057377551489921075 1103 74-75 -0.04884251723350275 1103 76-77 -0.09737655187634431 1103 78-79 -0.08853202075000155 1103 80-81 -0.15769524917371314 1103 82-83 -0.1284844465052828 1103 84-85 -0.1392354192100882 1103 86-87 -0.11248412437476674 1103 88-89 -0.14647831106493925 1103 90-91 -0.15722679694815156 1103 92-93 -0.14431184479585824 1103 94-95 -0.1695486762366727 1103 96-97 -0.16819454131266554 1103 98-99 -0.16399688341389407 1103 100 -0.1890008357219486 1104 1 0.04861696467152399 1104 2 0.07048338077660077 1104 3 0.058854099710096364 1104 4 -0.02516332616541206 1104 5 -0.002541064206603494 1104 6 0.0059331919142806555 1104 7 0.026770744514195144 1104 8 0.0292192448447679 1104 9 0.058917295795879454 1104 10-11 0.030114987407507954 1104 12-13 0.05868975749709193 1104 14-15 0.08222530576401255 1104 16-17 0.10626068011035983 1104 18-19 0.05903250096873336 1104 20-21 0.09827769541654163 1104 22-23 0.09916028818617661 1104 24-25 0.05017144364173731 1104 26-27 -7.511328695528618E-4 1104 28-29 0.04356553496829463 1104 30-31 0.04616978837167096 1104 32-33 0.02426889889224526 1104 34-35 0.014234163393595622 1104 36-37 -0.002187698065242216 1104 38-39 0.029978009629658686 1104 40-41 0.018040173221912426 1104 42-43 -0.025314117563752347 1104 44-45 0.011254821391759151 1104 46-47 0.028197653793760935 1104 48-49 -0.05566991817688205 1104 50-51 0.01171614123474285 1104 52-53 0.045501637399304684 1104 54-55 0.003661441052635439 1104 56-57 0.06874305380167556 1104 58-59 0.010451667385822816 1104 60-61 0.07645127635623084 1104 62-63 0.05384007450295769 1104 64-65 0.05934878636996643 1104 66-67 0.007208433061379083 1104 68-69 0.038826030931872424 1104 70-71 0.05762217652813462 1104 72-73 0.05679479710327939 1104 74-75 0.045547759373661734 1104 76-77 -0.0077044786809779 1104 78-79 -0.06501187948600773 1104 80-81 -0.01948431444910881 1104 82-83 -0.002949569345055636 1104 84-85 0.0039193198903646476 1104 86-87 -0.0030841132296757223 1104 88-89 -0.05179295083478763 1104 90-91 -0.02154170489669127 1104 92-93 -0.04442155425734029 1104 94-95 -0.08424190768532469 1104 96-97 0.037095805647858526 1104 98-99 -0.00617677558517471 1104 100 -0.03492521629884493 1105 1 0.009246935767961872 1105 2 0.013933242588031192 1105 3 0.050650973061856064 1105 4 0.01874266341242503 1105 5 0.004369208162010807 1105 6 -0.02745105669855974 1105 7 -0.04482106122010521 1105 8 -0.051819595637098814 1105 9 -0.020916455971033088 1105 10-11 -0.0519517228606432 1105 12-13 -0.04870195962774204 1105 14-15 -0.0123245792546669 1105 16-17 -0.025564041930188353 1105 18-19 -3.917484880062716E-4 1105 20-21 0.008345207389375275 1105 22-23 0.09454985336687827 1105 24-25 -0.02142500354069199 1105 26-27 0.045931919515524555 1105 28-29 0.011196075697007757 1105 30-31 0.06746243167637545 1105 32-33 0.07906878918529259 1105 34-35 0.09893700522570015 1105 36-37 -0.002248036890954097 1105 38-39 -0.0059898377932867675 1105 40-41 0.01696947552786554 1105 42-43 -0.0010836485665066675 1105 44-45 0.06838978204493884 1105 46-47 0.0960676510510865 1105 48-49 0.059685680042484535 1105 50-51 0.002017202438565846 1105 52-53 -0.050130970883564885 1105 54-55 -0.10158445021088625 1105 56-57 -0.08176518476876282 1105 58-59 -0.08678034172829285 1105 60-61 -0.12158771180807548 1105 62-63 -0.043832450233850295 1105 64-65 -0.14389917244773187 1105 66-67 -0.15260907363707332 1105 68-69 -0.2198804818637612 1105 70-71 -0.03552345945575297 1105 72-73 -0.05716267035383282 1105 74-75 -0.11858777091179462 1105 76-77 -0.0992631613536048 1105 78-79 -0.06373041785180078 1105 80-81 0.0031716487568687057 1105 82-83 0.012330288879802254 1105 84-85 -0.05603658635238418 1105 86-87 -0.03577784094544967 1105 88-89 -0.03571539645600552 1105 90-91 -0.03748085973118265 1105 92-93 -0.05731992764074789 1105 94-95 -0.07737066567599982 1105 96-97 -0.11238920458442436 1105 98-99 -0.1468141308036408 1105 100 -0.16064474624673153 1106 1 0.08065562843945884 1106 2 0.08416041422565002 1106 3 0.07142148767682244 1106 4 0.051465254483076706 1106 5 0.008107440491635032 1106 6 0.03225113503642518 1106 7 0.018887953417305425 1106 8 0.10395306133658977 1106 9 0.09008800421577945 1106 10-11 0.039386373990296875 1106 12-13 0.10706620859433968 1106 14-15 0.1739066240980236 1106 16-17 0.164299663248606 1106 18-19 0.13560253039405268 1106 20-21 0.11387485967434685 1106 22-23 0.1991058954305558 1106 24-25 0.12292100575509579 1106 26-27 0.11467902733124902 1106 28-29 0.12751060660205837 1106 30-31 0.13583366775614536 1106 32-33 0.17845248359108723 1106 34-35 0.20312423513547628 1106 36-37 0.1334974427321498 1106 38-39 0.19054941633942235 1106 40-41 0.19672336951988 1106 42-43 0.23425568065077584 1106 44-45 0.23174700097767698 1106 46-47 0.25378295810210716 1106 48-49 0.24021053752462507 1106 50-51 0.26001823893211906 1106 52-53 0.2277216103526385 1106 54-55 0.20610212882990453 1106 56-57 0.19809917331935623 1106 58-59 0.22290760842207646 1106 60-61 0.21200467690149338 1106 62-63 0.1376775249963984 1106 64-65 0.14332604454259723 1106 66-67 0.23217271182508625 1106 68-69 0.25170139500570343 1106 70-71 0.24975967875291616 1106 72-73 0.21925714217957193 1106 74-75 0.2122019229145451 1106 76-77 0.18429645946930862 1106 78-79 0.20469909038506273 1106 80-81 0.19486456101810035 1106 82-83 0.32275317008703297 1106 84-85 0.3112812213413392 1106 86-87 0.28452947531158657 1106 88-89 0.24318877685877283 1106 90-91 0.2300352691694343 1106 92-93 0.17590728072302397 1106 94-95 0.200968169778605 1106 96-97 0.16186605031545298 1106 98-99 0.20843591306370257 1106 100 0.22985272985066274 1107 1 -0.02765868707609087 1107 2 -0.02154082266540769 1107 3 -0.010305895153294387 1107 4 -0.014050886930441209 1107 5 -0.10949648474221618 1107 6 -0.09812489053152262 1107 7 -0.0930467120406746 1107 8 -0.04251688853260305 1107 9 -0.09812918647514834 1107 10-11 -0.08382557492193143 1107 12-13 -0.03977709401210916 1107 14-15 -0.0518718827503335 1107 16-17 -0.013763856080522885 1107 18-19 -0.06349504167278752 1107 20-21 -0.10082788046917557 1107 22-23 -0.04431872238668433 1107 24-25 -0.02730363855612694 1107 26-27 0.0163406500909673 1107 28-29 -0.011776013170042177 1107 30-31 -0.03221837449113707 1107 32-33 0.015169671079611646 1107 34-35 -0.021716242487343607 1107 36-37 -0.021304443652084615 1107 38-39 -0.05098043633620364 1107 40-41 -0.04325156273377928 1107 42-43 0.02086682190261513 1107 44-45 0.023771913134339684 1107 46-47 0.02276774952367333 1107 48-49 0.02079011844164569 1107 50-51 -0.006431190595840519 1107 52-53 -0.026213941466615154 1107 54-55 -0.02613206081422703 1107 56-57 -0.028738311857829046 1107 58-59 -0.07905430553714154 1107 60-61 -0.1046367559107182 1107 62-63 -0.07460963074777283 1107 64-65 -0.05810498213212156 1107 66-67 0.004469837180842262 1107 68-69 -0.0010300551822481907 1107 70-71 -0.045466694072125335 1107 72-73 -0.0588778765419562 1107 74-75 -0.09487121344996297 1107 76-77 -0.11055929437283396 1107 78-79 -0.061007343763563426 1107 80-81 -0.07200973298983371 1107 82-83 -0.044279361433545716 1107 84-85 -0.07646598230133606 1107 86-87 -0.07041443351661059 1107 88-89 -0.05417347824175067 1107 90-91 -0.04322198976849023 1107 92-93 0.014709736977877697 1107 94-95 0.017708994088337704 1107 96-97 0.021462262300453006 1107 98-99 0.032950089767034285 1107 100 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-0.09913481239719601 2214 3 -0.1325303048979265 2214 4 -0.1074748136996746 2214 5 -0.04454999090166467 2214 6 -0.04627012967753785 2214 7 -0.04529797614278408 2214 8 -0.013054031774380803 2214 9 -0.07057125162156552 2214 10-11 -0.05794362232573036 2214 12-13 -0.054683620364023966 2214 14-15 -0.10913657195368387 2214 16-17 -0.1118068445199043 2214 18-19 -0.1223530981912404 2214 20-21 -0.12835517231189186 2214 22-23 -0.14945793964437826 2214 24-25 -0.10684783952385857 2214 26-27 -0.16152835114147024 2214 28-29 -0.045962152460226946 2214 30-31 -0.11272490449944428 2214 32-33 -0.06381994332882357 2214 34-35 -0.11861384019301369 2214 36-37 -0.14041476174592304 2214 38-39 -0.16781641977637207 2214 40-41 -0.08036845498264 2214 42-43 -0.03268767387028504 2214 44-45 -0.1240561344931379 2214 46-47 -0.05598658888610686 2214 48-49 -0.1173746470541488 2214 50-51 -0.060648267900774044 2214 52-53 -0.10913683734955981 2214 54-55 -0.1368275657511404 2214 56-57 -0.05653941718066591 2214 58-59 -0.1656171063623475 2214 60-61 -0.15562225937745922 2214 62-63 -0.21957288579589118 2214 64-65 -0.22828601550032346 2214 66-67 -0.2224135428602807 2214 68-69 -0.16783886848612184 2214 70-71 -0.08305663130901308 2214 72-73 -0.1847914277954601 2214 74-75 -0.11538080568185904 2214 76-77 -0.10936155083413013 2214 78-79 -0.27524225841706595 2214 80-81 -0.20368728652386636 2214 82-83 -0.2330678004938065 2214 84-85 -0.27066964731623244 2214 86-87 -0.21192250730941709 2214 88-89 -0.24021287566650074 2214 90-91 -0.13303786647680482 2214 92-93 -0.14146411643856283 2214 94-95 -0.18055114769219927 2214 96-97 -0.30540067521682346 2214 98-99 -0.3603973783619878 2214 100 -0.5629588436738473 2215 1 0.020070779146642792 2215 2 -0.011277819180804727 2215 3 -0.03423454737361453 2215 4 -0.011432568335443705 2215 5 0.030571296540379933 2215 6 -0.02737184234678125 2215 7 0.013177117273407646 2215 8 -0.013138422812843942 2215 9 -0.039098267734573255 2215 10-11 -0.031050147751741974 2215 12-13 -0.014109821758061969 2215 14-15 0.002722299826956487 2215 16-17 -0.018458295712640904 2215 18-19 -0.09308877830282114 2215 20-21 -0.07844629365904865 2215 22-23 -0.08856206338040096 2215 24-25 -0.0952869149928901 2215 26-27 -0.06008184062454802 2215 28-29 -0.054746377406267754 2215 30-31 -0.07048632609856043 2215 32-33 -0.11305983176485768 2215 34-35 -0.13273299145605222 2215 36-37 -0.06802390687210647 2215 38-39 -0.06959052544728195 2215 40-41 -0.05832228321430222 2215 42-43 -0.05759959380835511 2215 44-45 -0.023087685637442235 2215 46-47 -0.10831088341279838 2215 48-49 -0.07961409897630034 2215 50-51 -0.03821493373669682 2215 52-53 -0.05760596046992106 2215 54-55 -0.10995420190707961 2215 56-57 -0.24876937140962951 2215 58-59 -0.22203266979714442 2215 60-61 -0.1726599648986351 2215 62-63 -0.11415799156721818 2215 64-65 -0.09994544436052877 2215 66-67 -0.17487674330898528 2215 68-69 -0.1205644646088686 2215 70-71 -0.06959946852091292 2215 72-73 -0.1015862129721512 2215 74-75 -0.07247354551095242 2215 76-77 -0.12600234704136426 2215 78-79 -0.12203705223026162 2215 80-81 -0.06948496197024667 2215 82-83 -0.044623382726577177 2215 84-85 -0.09740155129897232 2215 86-87 -0.08490538875999931 2215 88-89 -0.09513262028244185 2215 90-91 -0.07351491766638674 2215 92-93 -0.028832554634341534 2215 94-95 -0.011857749218194158 2215 96-97 0.033709629792618045 2215 98-99 0.01398415500343475 2215 100 0.05416290125646128 2216 1 -0.049318754700699685 2216 2 -0.10625245971054653 2216 3 -0.12683695628873437 2216 4 -0.06721824281478916 2216 5 -0.08401917100081135 2216 6 -0.09770684442019473 2216 7 -0.06402900652734189 2216 8 -0.06348231899569612 2216 9 -0.0853129235006449 2216 10-11 -0.08505535849896972 2216 12-13 -0.13442000851559754 2216 14-15 -0.2729522874131831 2216 16-17 -0.19924514293848716 2216 18-19 -0.1937838416968063 2216 20-21 -0.19420434761290295 2216 22-23 -0.19798986629142945 2216 24-25 -0.251374313691052 2216 26-27 -0.2910931984398246 2216 28-29 -0.2724093000406498 2216 30-31 -0.2532758725177473 2216 32-33 -0.3035457616214785 2216 34-35 -0.2211938461161722 2216 36-37 -0.29277010107517754 2216 38-39 -0.24856817665182973 2216 40-41 -0.32563647795026185 2216 42-43 -0.3388419123187987 2216 44-45 -0.2182658915041742 2216 46-47 -0.18399149856993802 2216 48-49 -0.1972139513040574 2216 50-51 -0.17656430711478777 2216 52-53 -0.1008277671750264 2216 54-55 -0.10954127786539658 2216 56-57 -0.10909186262341564 2216 58-59 -0.11415148496666205 2216 60-61 -0.11849490880382518 2216 62-63 -0.10087534746482163 2216 64-65 -0.08482452012370345 2216 66-67 -0.08927692100353823 2216 68-69 -0.08605804077264878 2216 70-71 -0.07035495181444418 2216 72-73 -0.07667778101865252 2216 74-75 -0.09737998379539903 2216 76-77 -0.110459703164139 2216 78-79 -0.02054510759140271 2216 80-81 0.02533886335995561 2216 82-83 0.06169769743862474 2216 84-85 0.0045245440931651615 2216 86-87 -0.010775261746857723 2216 88-89 -0.004738258472315238 2216 90-91 -0.027350455450402933 2216 92-93 0.02902266558868405 2216 94-95 0.016751753405941372 2216 96-97 0.08381827302812894 2216 98-99 0.08524011490113992 2216 100 0.11727167396318094 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 10.0 3 6.0 4 7.0 5 36.0 6 53.0 7 102.0 8 246.0 9 474.0 10 1018.0 11 1613.0 12 2402.0 13 3630.0 14 4625.0 15 6015.0 16 7522.0 17 10096.0 18 12130.0 19 6660.0 20 4297.0 21 4116.0 22 4306.0 23 4567.0 24 5162.0 25 6265.0 26 7680.0 27 10075.0 28 12906.0 29 16628.0 30 21301.0 31 27022.0 32 35077.0 33 45670.0 34 63436.0 35 97086.0 36 180754.0 37 423354.0 38 846035.0 39 473204.0 40 2049.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.12507983556219 20.501985549869833 13.961395828341391 23.411538786226586 2 15.30437990312697 20.62259941923889 40.685480418587595 23.387540259046542 3 17.837525850483573 26.012178213393476 30.599134874032803 25.551161062090145 4 11.996716694339918 17.265553318788193 39.9476238630042 30.790106123867687 5 12.202495095206684 38.06967618989178 36.416492378181296 13.31133633672024 6 29.858938709303672 39.118950499907996 17.907917424877493 13.114193365910843 7 26.13611240679092 33.09619197928048 23.27631632194484 17.49137929198375 8 24.561088894833425 36.458501943238026 20.81824718396611 18.162161977962437 9 24.606411907828825 16.566054645702565 22.02052962304143 36.80700382342718 10-11 23.282828390394545 26.984441723017945 29.52106916564613 20.211660720941378 12-13 24.323500033011946 24.420981115037048 29.67269187927425 21.58282697267676 14-15 21.460611292179976 26.32546775295207 27.273997410583895 24.939923544284063 16-17 20.54923359040055 29.833662387892495 27.73154685460048 21.88555716710647 18-19 20.287506424019583 28.369173310410968 30.581953877402977 20.761366388166472 20-21 21.503300129705 27.623054691210513 30.237600819548184 20.6360443595363 22-23 21.05811302623239 27.40461452220791 30.340083113537386 21.197189338022312 24-25 20.935214320125848 27.619691834285852 30.24299368768457 21.20210015790373 26-27 20.739889755800217 27.533426066080498 30.388844978917888 21.3378391992014 28-29 20.737287337754314 27.581883777369676 30.455499877952306 21.225329006923705 30-31 21.050535790681145 28.030259773684467 29.98069295708391 20.938511478550474 32-33 20.7056502723626 27.69278947446907 30.46779900769262 21.133761245475714 34-35 20.911684359137304 27.478375021010898 30.45396531268794 21.155975307163857 36-37 21.300086512801276 27.89474368159369 29.739888536744935 21.0652812688601 38-39 21.098616104923966 28.015918564031512 29.84434642156117 21.04111890948335 40-41 20.94073860240974 27.784352553812262 30.02438289128469 21.25052595249331 42-43 20.9794793586791 27.695889161397275 29.888465652333313 21.436165827590315 44-45 21.145551719458737 27.92565106127712 29.67662676647856 21.25217045278558 46-47 21.257343574931088 28.150569380008434 29.330270226516536 21.261816818543938 48-49 21.272361570570514 28.104601892848493 29.06212345540168 21.560913081179315 50-51 21.291983940191056 28.052673617397417 28.99193814630614 21.66340429610539 52-53 21.30139781437618 28.03219735533895 28.9949146376477 21.671490192637172 54-55 21.438496678236184 28.428854276888053 28.749912674842005 21.382736370033754 56-57 21.2336552741765 28.122118713937667 28.817833820252265 21.82639219163357 58-59 21.10487049726435 27.787313462240075 29.24913779498295 21.858678245512625 60-61 21.405545098169508 28.13946276723307 28.844984906009184 21.610007228588234 62-63 21.173240268451803 28.092640620171284 28.91954560585992 21.814573505516996 64-65 21.2234250329172 27.843690270353395 29.029369680906576 21.903515015822833 66-67 21.107243369144022 27.956277477261043 28.834101557192106 22.10237759640283 68-69 21.577967762594753 28.036672971648454 28.76594517541104 21.619414090345753 70-71 21.47809713342391 27.89327859435903 28.777022239270174 21.851602032946893 72-73 21.35835441851748 28.042570773804957 28.770648571745 21.82842623593256 74-75 21.39981896597625 28.025003993397586 28.682072307118894 21.89310473350727 76-77 21.355091470005355 28.13634190262297 28.54805543042923 21.960511196942445 78-79 21.518894870560132 28.199385381327456 28.605134231925796 21.676585516186613 80-81 21.41152220690407 27.98915665242313 28.72515909234672 21.874162048326077 82-83 21.29339817057431 28.021333928545566 28.8748358977723 21.810432003107827 84-85 21.229525619252446 27.977099447996256 28.717825516723465 22.075549416027833 86-87 21.383045291189287 28.10420376967249 28.53402003977627 21.978730899361953 88-89 21.123045107097145 27.980882888523983 28.688169157471243 22.207902846907633 90-91 21.0407708182916 27.903464550494434 28.756408044691785 22.299356586522183 92-93 21.1937332677354 28.038856125419837 28.577760171406542 22.189650435438217 94-95 20.952490485105223 27.784962313136415 28.742521729314824 22.520025472443546 96-97 20.868980910575964 27.82244684544233 28.756173766364874 22.552398477616837 98-99 20.884102511676645 27.845214439212228 28.688680310184505 22.582002738926622 100 21.009100648099043 27.79320465063777 28.58987875031681 22.607815950946378 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 44.0 1 38.0 2 91.0 3 150.0 4 100.5 5 71.0 6 180.5 7 438.0 8 802.5 9 984.0 10 816.0 11 655.0 12 740.5 13 887.5 14 1031.0 15 1194.5 16 1372.5 17 1625.5 18 1917.0 19 2292.5 20 2899.5 21 3670.5 22 4850.5 23 6788.0 24 9238.0 25 12187.0 26 16266.0 27 21787.0 28 28437.0 29 35598.5 30 42519.5 31 49664.5 32 57351.0 33 64285.0 34 71281.0 35 77153.5 36 81365.5 37 85693.5 38 88542.5 39 89209.0 40 89317.0 41 88935.0 42 87342.0 43 85724.5 44 83916.5 45 80955.0 46 78058.0 47 74855.0 48 72523.0 49 70926.5 50 67803.0 51 64238.0 52 59886.5 53 55898.5 54 53181.0 55 50549.5 56 46533.5 57 41824.0 58 37920.5 59 34183.0 60 30513.0 61 26315.0 62 22005.5 63 18056.0 64 14414.5 65 11550.5 66 9053.5 67 6826.0 68 5158.5 69 3998.5 70 2946.5 71 2102.5 72 1601.0 73 1226.5 74 959.5 75 702.0 76 466.5 77 343.5 78 255.0 79 177.0 80 105.0 81 46.0 82 26.5 83 8.5 84 5.0 85 1.5 86 0.0 87 0.5 88 0.5 89 0.0 90 0.0 91 1.0 92 1.5 93 1.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.093242774110967 2 0.0011074975454020749 3 0.0 4 4.259605943854134E-5 5 0.04050885252605282 6 1.2778817831562403E-4 7 0.0038336453494687206 8 2.981724160697894E-4 9 1.2778817831562403E-4 10-11 4.259605943854134E-4 12-13 0.0 14-15 6.389408915781201E-5 16-17 0.0 18-19 2.129802971927067E-5 20-21 0.0 22-23 1.2778817831562403E-4 24-25 8.519211887708268E-5 26-27 0.0012139876939984283 28-29 0.008178443412199938 30-31 0.03505655691791952 32-33 0.024705714474353976 34-35 0.027197583951508644 36-37 0.02485480068238887 38-39 0.024024177523337315 40-41 0.029923731755575292 42-43 0.021745288343375354 44-45 0.020722982916850364 46-47 0.014695640506296763 48-49 0.009392431106198366 50-51 0.006815369510166615 52-53 0.004813354716555172 54-55 0.0037058571711530964 56-57 0.002151101001646338 58-59 8.732192184900974E-4 60-61 5.111527132624961E-4 62-63 0.0010861995156828041 64-65 0.0033224926362062247 66-67 5.111527132624961E-4 68-69 8.732192184900974E-4 70-71 3.6206650522760137E-4 72-73 6.389408915781201E-4 74-75 4.259605943854134E-4 76-77 3.1947044578906005E-4 78-79 2.129802971927067E-5 80-81 8.732192184900974E-4 82-83 5.537487727010374E-4 84-85 2.5557635663124806E-4 86-87 1.7038423775416535E-4 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2347635.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.58041610797368 #Duplication Level Percentage of deduplicated Percentage of total 1 77.75911126481788 52.54993095462615 2 14.03210741669771 18.96591316184429 3 4.209035942839198 8.533452012914706 4 1.6946964466596426 4.581131641678522 5 0.7622983384401592 2.5758219455101448 6 0.43585533691067946 1.7673171018782863 7 0.25452019559823924 1.2040406508488302 8 0.17392645451827335 0.9403217734823576 9 0.1268303640124726 0.77141238975798 >10 0.5239207604512293 6.01842749270834 >50 0.021806596965980134 1.0116777496232967 >100 0.005638182974164479 0.6521210602703948 >500 6.317477861269537E-5 0.03979060133995164 >1k 1.895243358380861E-4 0.38864146351671647 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4102 0.17472903581689658 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3285 0.1399280552556083 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 4.259605943854134E-5 0.0 5 0.0 0.0 0.0 4.259605943854134E-5 0.0 6 0.0 0.0 0.0 4.259605943854134E-5 0.0 7 0.0 0.0 0.0 4.259605943854134E-5 0.0 8 0.0 0.0 0.0 4.259605943854134E-5 0.0 9 0.0 0.0 0.0 4.259605943854134E-5 0.0 10-11 0.0 0.0 0.0 4.259605943854134E-5 0.0 12-13 0.0 0.0 0.0 4.259605943854134E-5 0.0 14-15 0.0 0.0 0.0 8.519211887708268E-5 0.0 16-17 0.0 0.0 0.0 1.2778817831562403E-4 0.0 18-19 0.0 0.0 0.0 1.2778817831562403E-4 0.0 20-21 0.0 0.0 0.0 1.7038423775416535E-4 0.0 22-23 0.0 0.0 0.0 2.5557635663124806E-4 0.0 24-25 0.0 0.0 0.0 3.1947044578906005E-4 0.0 26-27 0.0 0.0 0.0 4.685566538239548E-4 0.0 28-29 0.0 0.0 0.0 0.0013843719317525936 4.259605943854134E-5 30-31 0.0 0.0 0.0 0.006112534529430683 4.259605943854134E-5 32-33 0.0 0.0 0.0 0.013779825228368124 4.259605943854134E-5 34-35 0.0 0.0 0.0 0.026665133208526877 4.259605943854134E-5 36-37 0.0 0.0 0.0 0.047281625976780886 4.259605943854134E-5 38-39 0.0 0.0 0.0 0.08451058192606602 4.259605943854134E-5 40-41 0.0 0.0 0.0 0.12731962166180005 4.259605943854134E-5 42-43 0.0 0.0 0.0 0.16829703084167685 4.259605943854134E-5 44-45 0.0 0.0 0.0 0.21932711004904937 4.259605943854134E-5 46-47 0.0 0.0 0.0 0.28004779277869 4.259605943854134E-5 48-49 0.0 0.0 0.0 0.3497775420795822 4.259605943854134E-5 50-51 0.0 0.0 0.0 0.4238733874729249 4.259605943854134E-5 52-53 0.0 0.0 0.0 0.5000990358381946 4.259605943854134E-5 54-55 0.0 0.0 0.0 0.5787100635320226 4.259605943854134E-5 56-57 0.0 0.0 0.0 0.6752966283089152 4.259605943854134E-5 58-59 0.0 0.0 0.0 0.7854500380169831 4.259605943854134E-5 60-61 0.0 0.0 0.0 0.9074664502786847 4.259605943854134E-5 62-63 0.0 0.0 0.0 1.026778012766039 4.259605943854134E-5 64-65 0.0 0.0 0.0 1.1530327329418755 4.259605943854134E-5 66-67 0.0 0.0 0.0 1.2975824606465656 4.259605943854134E-5 68-69 0.0 0.0 0.0 1.4630255555058602 4.259605943854134E-5 70-71 0.0 0.0 0.0 1.6427383302770662 4.259605943854134E-5 72-73 0.0 0.0 0.0 1.8317157479761548 4.259605943854134E-5 74-75 0.0 0.0 0.0 2.0239943602817303 4.259605943854134E-5 76-77 0.0 0.0 0.0 2.228774916032518 4.259605943854134E-5 78-79 0.0 0.0 0.0 2.4664396296698596 4.259605943854134E-5 80-81 0.0 0.0 0.0 2.726424678452996 4.259605943854134E-5 82-83 0.0 0.0 0.0 2.996057734698963 4.259605943854134E-5 84-85 0.0 0.0 0.0 3.267458527411629 4.259605943854134E-5 86-87 0.0 0.0 0.0 3.5491888645381415 4.259605943854134E-5 88 0.0 0.0 0.0 3.785511802303169 4.259605943854134E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2915 0.0 50.849426 1 GTATCAA 5735 0.0 45.045666 1 TCAACGC 6725 0.0 37.68094 4 ATCAACG 6840 0.0 37.047413 3 CAACGCA 6895 0.0 36.752674 5 AACGCAG 7215 0.0 35.237926 6 TATCAAC 7240 0.0 35.195404 2 ACGCAGA 8340 0.0 30.540958 7 CGCAGAG 8405 0.0 30.13639 8 TAGACCG 65 0.006146004 28.932177 5 GCAGAGT 9545 0.0 26.192434 9 GTACATG 5310 0.0 24.90152 1 TGGTATC 1495 0.0 23.899984 2 GTGGTAT 1755 0.0 23.326855 1 TACATGG 5645 0.0 22.486666 2 AGAGTAC 8860 0.0 21.693508 10-11 ACATGGG 5650 0.0 21.634663 3 GAGTACT 6960 0.0 21.437069 12-13 GTAACGG 135 4.0707318E-4 20.895016 3 GGACCGT 180 5.6743374E-6 20.886559 6 >>END_MODULE