FastQCFastQC Report
Wed 25 May 2016
SRR1033114_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033114_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3457449
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28000.035.651871
GTATCAA43100.033.867951
TCAACGC44250.031.4168784
CAACGCA46000.030.2207975
ATCAACG47100.029.6198563
AACGCAG48900.028.5200656
GTACATG104150.026.85541
TACATGG104200.024.8997732
TATCAAC57300.024.608812
ACATGGG109750.022.8979553
ACGCAGA60200.022.6986167
CGCAGAG61550.021.8196188
GTACTAG7000.019.5076271
CATGGGG74900.019.0623464
GAGTACA75150.018.2960911
CTAGTAC7100.017.2013453
ACCGTCC3602.226443E-916.957048
GCAGAGT77000.016.7706999
TATACCG2003.0116687E-416.4376645
AGTACAT78700.016.4241162
Produced by FastQC (version 0.11.5)