##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033114_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3457449
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.72149986883393	33.0	31.0	34.0	30.0	34.0
2	31.884580799311863	34.0	31.0	34.0	30.0	34.0
3	32.00778869044778	34.0	31.0	34.0	30.0	34.0
4	35.36849422796981	37.0	35.0	37.0	33.0	37.0
5	35.43317949158469	37.0	35.0	37.0	35.0	37.0
6	35.54595830625412	37.0	37.0	37.0	35.0	37.0
7	35.4940191453294	37.0	37.0	37.0	35.0	37.0
8	35.4989782929553	37.0	37.0	37.0	35.0	37.0
9	37.18592320523022	39.0	38.0	39.0	35.0	39.0
10-11	37.17204693402563	39.0	38.0	39.0	35.0	39.0
12-13	37.12367687853096	39.0	38.0	39.0	35.0	39.0
14-15	38.59613041291426	41.0	39.0	41.0	35.0	41.0
16-17	38.597592473526	41.0	39.0	41.0	35.0	41.0
18-19	38.58404071903881	41.0	39.0	41.0	35.0	41.0
20-21	38.605128521057	41.0	39.0	41.0	35.0	41.0
22-23	38.56747908645941	41.0	39.0	41.0	34.5	41.0
24-25	38.549956051412465	41.0	39.0	41.0	34.0	41.0
26-27	38.49995140926157	41.0	39.0	41.0	34.0	41.0
28-29	38.46543289575638	40.5	39.0	41.0	34.0	41.0
30-31	38.460153859102476	41.0	39.0	41.0	34.0	41.0
32-33	38.52711666896605	41.0	39.0	41.0	34.0	41.0
34-35	38.49688382966748	41.0	39.0	41.0	34.0	41.0
36-37	38.41422013744816	41.0	39.0	41.0	34.0	41.0
38-39	38.334997132278744	41.0	39.0	41.0	34.0	41.0
40-41	38.271687593945714	41.0	38.5	41.0	34.0	41.0
42-43	38.15588270427127	40.5	38.0	41.0	33.0	41.0
44-45	38.025879774365436	40.0	38.0	41.0	33.0	41.0
46-47	37.895400337069326	40.0	38.0	41.0	33.0	41.0
48-49	37.76955943529464	40.0	38.0	41.0	33.0	41.0
50-51	37.63591755077226	40.0	37.5	41.0	33.0	41.0
52-53	37.479872298911715	40.0	37.0	41.0	32.0	41.0
54-55	37.31518122176206	40.0	37.0	41.0	32.0	41.0
56-57	37.143610650511405	40.0	36.0	41.0	32.0	41.0
58-59	36.93292872866671	40.0	36.0	41.0	31.0	41.0
60-61	36.69807681906516	39.0	35.0	41.0	31.0	41.0
62-63	36.42061169955074	39.0	35.0	41.0	31.0	41.0
64-65	36.13564639709797	38.5	35.0	41.0	31.0	41.0
66-67	35.82889740383734	38.0	35.0	40.0	30.0	41.0
68-69	35.50754718290855	37.0	35.0	40.0	30.0	41.0
70-71	35.148467844355764	37.0	35.0	39.5	30.0	41.0
72-73	34.77561360991876	36.0	34.5	39.0	29.0	41.0
74-75	34.40449042053838	36.0	34.0	39.0	29.0	40.5
76-77	34.074888017147906	35.0	34.0	37.5	29.0	40.0
78-79	33.714609817816545	35.0	34.0	37.0	29.0	39.0
80-81	33.368753667805365	35.0	34.0	37.0	28.5	39.0
82-83	33.16505507384201	35.0	34.0	36.0	29.0	38.5
84-85	32.91115183477761	35.0	34.0	36.0	28.5	37.0
86-87	32.67568082710692	35.0	34.0	36.0	28.0	37.0
88-89	32.33410167438478	35.0	34.0	35.0	27.0	36.5
90-91	32.15811310593446	35.0	34.0	35.0	27.0	36.0
92-93	31.992891579890262	35.0	34.0	35.0	27.0	36.0
94-95	31.83101023326736	35.0	33.0	35.0	26.5	36.0
96-97	31.661128913253673	35.0	33.0	35.0	25.5	35.5
98-99	31.472830691067315	35.0	33.0	35.0	25.0	35.0
100	31.31173243625575	35.0	33.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.19161178954339775
1101	2	0.2635392050839549
1101	3	0.2573309865087623
1101	4	0.20450306471736468
1101	5	0.10782873514181546
1101	6	0.15803718101400221
1101	7	0.20069040079710732
1101	8	0.15394360151808684
1101	9	0.25120790016087824
1101	10-11	0.21251390714679275
1101	12-13	0.21943082259411995
1101	14-15	0.24526721748022595
1101	16-17	0.20948467423291817
1101	18-19	0.22509846916445042
1101	20-21	0.1604370025438726
1101	22-23	0.24081454997981666
1101	24-25	0.2552079451382383
1101	26-27	0.2588188243864735
1101	28-29	0.3021257073594015
1101	30-31	0.29101260236242155
1101	32-33	0.2616021118553675
1101	34-35	0.303723523182029
1101	36-37	0.2750350560157315
1101	38-39	0.2648804811200165
1101	40-41	0.2571566670939589
1101	42-43	0.19987487784095492
1101	44-45	0.18591032019048015
1101	46-47	0.18477748137330252
1101	48-49	0.24830971437232563
1101	50-51	0.23966427805249424
1101	52-53	0.17966542766775717
1101	54-55	0.11205382811304077
1101	56-57	0.15185447013021047
1101	58-59	0.2014380245281302
1101	60-61	0.08785780536086207
1101	62-63	0.12046467307581565
1101	64-65	0.09069600022220214
1101	66-67	0.083684177212632
1101	68-69	0.15715462888833542
1101	70-71	0.15432965108129082
1101	72-73	0.17926913456346938
1101	74-75	0.09790647627707472
1101	76-77	0.05584242947307416
1101	78-79	0.08148623963258217
1101	80-81	0.09460682736546744
1101	82-83	0.038736326014884526
1101	84-85	-0.022185036841641192
1101	86-87	0.09354327180253819
1101	88-89	0.268356798093194
1101	90-91	0.2170560524530316
1101	92-93	0.22667532696686976
1101	94-95	0.20254986526943597
1101	96-97	0.22681453555060926
1101	98-99	0.2820745208555806
1101	100	0.33269236780890665
1102	1	0.21056762293441622
1102	2	0.19353985576156063
1102	3	0.20521546486167352
1102	4	0.19654089996376456
1102	5	0.06441533076785788
1102	6	0.10704252112027746
1102	7	0.10280432556737651
1102	8	0.13927923653326957
1102	9	0.1847957148466648
1102	10-11	0.16815132184254367
1102	12-13	0.19959836346433946
1102	14-15	0.19782265991692327
1102	16-17	0.19900350808082834
1102	18-19	0.1393540469359138
1102	20-21	0.146117081459181
1102	22-23	0.1739272551602653
1102	24-25	0.19542474225921325
1102	26-27	0.22757055129942927
1102	28-29	0.24748692155827712
1102	30-31	0.21722774942152512
1102	32-33	0.25493949119895376
1102	34-35	0.26023924221458117
1102	36-37	0.25705197018800163
1102	38-39	0.24366454799111636
1102	40-41	0.2913799459887372
1102	42-43	0.24216934201457008
1102	44-45	0.17237666767909587
1102	46-47	0.216370375055007
1102	48-49	0.2005790221039092
1102	50-51	0.16437937689608617
1102	52-53	0.19156041510455424
1102	54-55	0.20364107463755943
1102	56-57	0.14637598995584966
1102	58-59	0.1795050429082039
1102	60-61	0.15820981487904362
1102	62-63	0.2308754750485562
1102	64-65	0.17382437158144626
1102	66-67	0.139179335141975
1102	68-69	0.12823384241568192
1102	70-71	0.16846287230782764
1102	72-73	0.12386459884137935
1102	74-75	0.17725513076369026
1102	76-77	0.0828330932433019
1102	78-79	0.17498176499820772
1102	80-81	0.20909242607655187
1102	82-83	0.08975680539685982
1102	84-85	0.12843247299439753
1102	86-87	0.17247910160577362
1102	88-89	0.23029460965811666
1102	90-91	0.23304218087223205
1102	92-93	0.25270815177481154
1102	94-95	0.22234263439033342
1102	96-97	0.28698257911676706
1102	98-99	0.3049708410550451
1102	100	0.3140202616600227
1103	1	0.08647152717521323
1103	2	0.038499009879547685
1103	3	0.05049677111399831
1103	4	0.027923685200775594
1103	5	0.009983773541975438
1103	6	0.08480671476681323
1103	7	0.03572958253268155
1103	8	0.06167890235514051
1103	9	0.08399061072621095
1103	10-11	0.07996785873046974
1103	12-13	0.08227810744241992
1103	14-15	0.13722897105613896
1103	16-17	0.06642949085518524
1103	18-19	0.08540899000154667
1103	20-21	0.10986164426546452
1103	22-23	0.09194909827332509
1103	24-25	0.08264757073880702
1103	26-27	0.15135698835886302
1103	28-29	0.14582661209504977
1103	30-31	0.15279422044270774
1103	32-33	0.1266745190499705
1103	34-35	0.13174078307150694
1103	36-37	0.1306876998810722
1103	38-39	0.12936838305058984
1103	40-41	0.19119942860756112
1103	42-43	0.09604787353457311
1103	44-45	0.0830900640405261
1103	46-47	0.08024637389236489
1103	48-49	0.14552177327719562
1103	50-51	0.10427242204752218
1103	52-53	0.06583260879867936
1103	54-55	0.11603373308745546
1103	56-57	0.09520175583737966
1103	58-59	0.08717046618066604
1103	60-61	0.10287331229281449
1103	62-63	0.18207743694273404
1103	64-65	0.22344706135841363
1103	66-67	0.15814366766111476
1103	68-69	0.07699463393850436
1103	70-71	0.1437932684091976
1103	72-73	0.19778750755091323
1103	74-75	0.2154312847915847
1103	76-77	0.17508639890409938
1103	78-79	0.15823567578318887
1103	80-81	0.204652858604355
1103	82-83	0.1920586357275198
1103	84-85	0.15153636057727482
1103	86-87	0.1959471898043219
1103	88-89	0.245742486539136
1103	90-91	0.27476470678973186
1103	92-93	0.3161420147617804
1103	94-95	0.24145461465920803
1103	96-97	0.23118877008103667
1103	98-99	0.3272348851203226
1103	100	0.3264780431999945
1104	1	0.0698048605085475
1104	2	0.11779729056727106
1104	3	0.15289581149784937
1104	4	0.11484558310827708
1104	5	0.01691766665140193
1104	6	0.028852429814122615
1104	7	0.08387365823573134
1104	8	0.07011885970553067
1104	9	0.11749054408619486
1104	10-11	0.10897292337127595
1104	12-13	0.11765562310282007
1104	14-15	0.024565703030013708
1104	16-17	0.10836771556530067
1104	18-19	0.12409851419186424
1104	20-21	0.08091322363371489
1104	22-23	0.029828946334106377
1104	24-25	0.03570301522767494
1104	26-27	0.10076056026343139
1104	28-29	0.11733467553634114
1104	30-31	0.13010662881269042
1104	32-33	0.1424765315782892
1104	34-35	0.09596842534793382
1104	36-37	0.11524887541086315
1104	38-39	0.09328115127662784
1104	40-41	0.05501390281788332
1104	42-43	0.008786111572689492
1104	44-45	0.023252332466491055
1104	46-47	-0.008308204276374909
1104	48-49	-0.08928763629231184
1104	50-51	-0.029242505314861944
1104	52-53	-0.10561881063353695
1104	54-55	-0.08631032928759197
1104	56-57	-0.05965796694017911
1104	58-59	-0.0813171387773508
1104	60-61	-0.08511982378608707
1104	62-63	-0.1033717167291357
1104	64-65	-0.13485295196957736
1104	66-67	-0.03777296568554789
1104	68-69	-0.00851616174322345
1104	70-71	-0.03174318367663176
1104	72-73	-0.0031437865980876722
1104	74-75	-0.13501187127931757
1104	76-77	-0.14380604411869058
1104	78-79	-0.1122727874981706
1104	80-81	-0.07971839289504601
1104	82-83	0.001586491251984512
1104	84-85	0.030708025244742032
1104	86-87	-0.022220543102520196
1104	88-89	0.12094240657112465
1104	90-91	0.06329429495447414
1104	92-93	0.12352072994237773
1104	94-95	0.123235235744108
1104	96-97	0.15915091855498176
1104	98-99	0.15807421605461514
1104	100	0.152324371335407
1105	1	0.05583412115388953
1105	2	0.07330385925079241
1105	3	0.09131350732369015
1105	4	0.046514733986043666
1105	5	-0.016330135540677304
1105	6	0.0287573081528123
1105	7	0.016014852316594386
1105	8	-0.006204050283912466
1105	9	0.07609401235987434
1105	10-11	0.04542039023991151
1105	12-13	0.0692928596446194
1105	14-15	0.0871911525562652
1105	16-17	0.10976441340194043
1105	18-19	0.07817406602091381
1105	20-21	0.03667800766719154
1105	22-23	0.18035554737308956
1105	24-25	0.1587590642170582
1105	26-27	0.15264449021095317
1105	28-29	0.18086446644184662
1105	30-31	0.14894796548986022
1105	32-33	0.17529638423687288
1105	34-35	0.13774442511851248
1105	36-37	0.1730368321466429
1105	38-39	0.0840219703712961
1105	40-41	0.09562532824575243
1105	42-43	0.08805413001688578
1105	44-45	0.0973716559305231
1105	46-47	0.14515474916658633
1105	48-49	0.08550554657171006
1105	50-51	0.15968214038633022
1105	52-53	0.12285836600511146
1105	54-55	0.11126161195354456
1105	56-57	0.10292121067742954
1105	58-59	0.11172602412601407
1105	60-61	0.0690361505084951
1105	62-63	0.18385914832245476
1105	64-65	0.11279323744804515
1105	66-67	0.06064022635685973
1105	68-69	0.06318478750670664
1105	70-71	0.06372296065144667
1105	72-73	0.03231651487803333
1105	74-75	0.10241013032960211
1105	76-77	0.08453397397950368
1105	78-79	0.08283825293770519
1105	80-81	0.03693999959745753
1105	82-83	0.09713904036755139
1105	84-85	0.05267019863523359
1105	86-87	0.08850124695864281
1105	88-89	0.06747874402516629
1105	90-91	0.08378468786256121
1105	92-93	0.11160791717212959
1105	94-95	0.18874410960001242
1105	96-97	0.2707494302379061
1105	98-99	0.2566966914226896
1105	100	0.32809031814108636
1106	1	0.15496117598922865
1106	2	0.08372829121016423
1106	3	0.1633996664659776
1106	4	0.14288820340265573
1106	5	0.19064130434409066
1106	6	0.11705487315904861
1106	7	0.11718335841064942
1106	8	0.10856167272923756
1106	9	0.12525147178842388
1106	10-11	0.1050623837306972
1106	12-13	0.09746205868056279
1106	14-15	0.1461710127961453
1106	16-17	0.1123240559004941
1106	18-19	0.12386378563197553
1106	20-21	0.12095226719917918
1106	22-23	0.14271193365081558
1106	24-25	0.17644221401170057
1106	26-27	0.1833127245828905
1106	28-29	0.19925116438180623
1106	30-31	0.1957432607578511
1106	32-33	0.1952103826732099
1106	34-35	0.17094351358679205
1106	36-37	0.12340613179048177
1106	38-39	0.14586450892250014
1106	40-41	0.187018909139141
1106	42-43	0.19940211412165354
1106	44-45	0.2085357983088727
1106	46-47	0.21076048745273823
1106	48-49	0.17984110014743493
1106	50-51	0.16122105725552416
1106	52-53	0.11819666508529991
1106	54-55	0.16510349998787177
1106	56-57	0.17531646634874676
1106	58-59	0.13169860948615053
1106	60-61	0.15180129169289103
1106	62-63	0.17939139032281304
1106	64-65	0.19317601208435065
1106	66-67	0.2056155895452747
1106	68-69	0.15138639117222397
1106	70-71	0.13377827478847593
1106	72-73	0.151042915964112
1106	74-75	0.1265812768756973
1106	76-77	0.1114501199296285
1106	78-79	0.1292926329163322
1106	80-81	0.1403312733781945
1106	82-83	0.12374255437083548
1106	84-85	0.09648160126679528
1106	86-87	0.04001248655694667
1106	88-89	0.16477344231260815
1106	90-91	0.14606763846165904
1106	92-93	0.20509295023333607
1106	94-95	0.19556342550676575
1106	96-97	0.13923643303189337
1106	98-99	0.13034338398881573
1106	100	0.23371991544689408
1107	1	0.18395211632037345
1107	2	0.20599563821977895
1107	3	0.16215699924393334
1107	4	0.20953404324437486
1107	5	0.16821081387236347
1107	6	0.1868420924308012
1107	7	0.10328145275616407
1107	8	0.11270621089439459
1107	9	0.2006549915218443
1107	10-11	0.20782367360538956
1107	12-13	0.22130183097394251
1107	14-15	0.13283143141532605
1107	16-17	0.13409155911129034
1107	18-19	0.1836680134774369
1107	20-21	0.21124002449725054
1107	22-23	0.18313506531106327
1107	24-25	0.21510027225738781
1107	26-27	0.23621633109474516
1107	28-29	0.27407673972284385
1107	30-31	0.2655183310483906
1107	32-33	0.26084438593608184
1107	34-35	0.26389536890977183
1107	36-37	0.2668630376185135
1107	38-39	0.20884135643542834
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2216	32-33	-0.32031708502964307
2216	34-35	-0.30960946944280465
2216	36-37	-0.3203278546592614
2216	38-39	-0.29512171460753933
2216	40-41	-0.2797484944999553
2216	42-43	-0.3043723163204817
2216	44-45	-0.23223252348616796
2216	46-47	-0.16429772191998637
2216	48-49	-0.17546556621835663
2216	50-51	-0.23628604023417665
2216	52-53	-0.20890283208067473
2216	54-55	-0.20930826397452762
2216	56-57	-0.2912335750305388
2216	58-59	-0.27817430742288707
2216	60-61	-0.22906211769041818
2216	62-63	-0.1771826573940416
2216	64-65	-0.15692105029137338
2216	66-67	-0.28741972413056516
2216	68-69	-0.20525149957966704
2216	70-71	-0.23100919017569765
2216	72-73	-0.19844714434428568
2216	74-75	-0.18473218983685769
2216	76-77	-0.20712975387263555
2216	78-79	-0.2059315455353996
2216	80-81	-0.18946052865820917
2216	82-83	-0.19317004824428352
2216	84-85	-0.1878101544363986
2216	86-87	-0.20547785538832386
2216	88-89	-0.19581279026827048
2216	90-91	-0.22409036364777535
2216	92-93	-0.23642203604827472
2216	94-95	-0.26897776441026267
2216	96-97	-0.3073231086595811
2216	98-99	-0.34143871896191413
2216	100	-0.3388099031963421
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	30143.0
3	5336.0
4	3483.0
5	3028.0
6	2641.0
7	3089.0
8	3724.0
9	4132.0
10	4369.0
11	4727.0
12	5308.0
13	5923.0
14	6509.0
15	7012.0
16	7015.0
17	7087.0
18	6913.0
19	6777.0
20	6753.0
21	7142.0
22	8154.0
23	10948.0
24	16757.0
25	22700.0
26	23316.0
27	20629.0
28	23213.0
29	29182.0
30	36115.0
31	44399.0
32	58673.0
33	81321.0
34	114492.0
35	177827.0
36	343432.0
37	728482.0
38	1129056.0
39	456742.0
40	900.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.2560451576059	19.237135468174706	13.361591659353975	24.14522771486542
2	15.551438469038406	19.79770999771527	40.26030887608268	24.39054265716365
3	18.52916286075155	24.929930642623702	29.47812944626395	27.062777050360793
4	12.475278694292609	16.427012867665674	38.376465230161685	32.72124320788003
5	13.303104835209353	37.845799936340754	35.34509100379062	13.506004224659279
6	31.632207660575922	37.372823430485	17.687526232223917	13.307442676715157
7	27.985098911054244	32.08281159563658	22.204664645954512	17.727424847354666
8	25.321182604590454	36.47897314134696	20.075952179286023	18.123892074776563
9	25.270206080410645	17.09306220829286	20.5599619996031	37.076769711693395
10-11	24.2949912669914	26.698286305268372	28.502659680259786	20.504062747480447
12-13	24.900116188199718	23.92836165092251	28.49671446153285	22.674807699344917
14-15	22.145538074655597	25.9009832879665	25.44339516616498	26.510083471212926
16-17	22.148016886840608	29.151641219292724	26.0728317684593	22.627510125407372
18-19	22.08023712247731	28.283931370793447	27.7372808617502	21.89855064497904
20-21	23.18212122610416	27.56540006691565	27.299386490820943	21.95309221615925
22-23	22.849456300063352	27.27374763875992	27.26211862175899	22.614677439417736
24-25	22.707985324162767	27.39859444315543	27.25099130177176	22.642428930910043
26-27	22.403786209899167	27.485141411526126	27.332990044130984	22.77808233444372
28-29	22.449895567551305	27.53402009963029	27.360722984974455	22.655361347843947
30-31	22.677373960066497	27.762176354439394	27.07995017894588	22.480499506548234
32-33	22.421914959602105	27.50950640742275	27.48004117321681	22.588537459758328
34-35	22.568716788578982	27.351653181896218	27.466610855979063	22.613019173545734
36-37	22.85561350531303	27.548806251226637	27.06914308714481	22.52643715631552
38-39	22.598816370180675	27.74382244916319	27.215068124233984	22.442293056422155
40-41	22.403757109772265	27.47010486023721	27.463654414628568	22.662483615361953
42-43	22.450696525471773	27.398674056161703	27.442120121718403	22.708509296648117
44-45	22.498583003182837	27.603387255142298	27.371388827542344	22.52664091413252
46-47	22.591594875899332	27.6356585895967	27.282072850116844	22.490673684387126
48-49	22.564926166935543	27.48772341362497	27.191986214646285	22.755364204793203
50-51	22.482649723195347	27.440865446428585	27.29637079949896	22.780114030877108
52-53	22.52801416183132	27.41779427163179	27.333534846319836	22.720656720217054
54-55	22.607508131632734	27.732067347316853	27.162032336210746	22.498392184839666
56-57	22.473615645731186	27.487076814857154	27.27370542542385	22.765602113987814
58-59	22.39283797559776	27.168827307300557	27.557535815400623	22.88079890170106
60-61	22.68912849561454	27.49756170364894	27.191198299729386	22.622111501007137
62-63	22.410517978114203	27.591036677878026	27.21579333806045	22.78265200594732
64-65	22.46123142557576	27.35372934668643	27.30898441736867	22.87605481036914
66-67	22.333664797624564	27.377793512208353	27.255721519089853	23.032820171077223
68-69	22.662469998663713	27.53821862202973	27.148555568346357	22.6507558109602
70-71	22.613077099623137	27.374782149813825	27.231320272999852	22.780820477563186
72-73	22.4444778351703	27.418937545346832	27.296576121719895	22.840008497762973
74-75	22.532544180168955	27.48428939048302	27.20440958564831	22.778756843699718
76-77	22.544542752640083	27.43168886808282	27.133814022953295	22.8899543563238
78-79	22.616430685360058	27.547452720823202	27.172091460014304	22.664025133802443
80-81	22.51551559918978	27.432782609223832	27.283375278068867	22.768326513517522
82-83	22.466508849143505	27.415417245804143	27.36623243949842	22.75184146555393
84-85	22.469703604676656	27.343664583949174	27.25871493187541	22.92791687949876
86-87	22.611976107830035	27.490413947955194	27.153770484880546	22.74383945933423
88-89	22.411608125245408	27.44616210949008	27.19514872332183	22.94708104194269
90-91	22.414051736643135	27.28771609374129	27.30306601148465	22.995166158130925
92-93	22.550097530379766	27.45894478050609	27.20773876320101	22.78321892591314
94-95	22.407003830710888	27.205844594432417	27.371784362384403	23.01536721247229
96-97	22.445427488311605	27.229668593639534	27.328107134648562	22.9967967834003
98-99	22.3932376123628	27.38929914124601	27.213693514941824	23.003769731449363
100	22.589232410994516	27.129317899083095	27.181773325063048	23.099676364859345
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	349.0
1	328.0
2	293.5
3	268.5
4	232.0
5	195.0
6	172.5
7	177.0
8	183.0
9	173.0
10	173.0
11	186.5
12	209.5
13	242.5
14	268.0
15	313.5
16	380.0
17	483.5
18	655.5
19	948.0
20	1490.5
21	2230.0
22	3331.5
23	5264.5
24	7752.5
25	11019.0
26	15668.0
27	21288.5
28	28632.0
29	37141.0
30	45327.0
31	54357.0
32	64133.0
33	74040.5
34	83786.0
35	92448.0
36	99241.0
37	105786.0
38	111954.0
39	115844.5
40	118867.5
41	121314.0
42	122341.0
43	124043.5
44	124339.0
45	122817.5
46	122203.0
47	119823.5
48	117444.5
49	116176.0
50	113553.0
51	110265.5
52	104705.5
53	99499.0
54	96730.5
55	92646.0
56	85803.0
57	77743.0
58	70718.0
59	65382.5
60	60228.0
61	53047.0
62	45681.5
63	38811.0
64	32177.5
65	26450.5
66	21218.0
67	16244.5
68	12406.0
69	9668.5
70	7144.0
71	5367.5
72	4297.0
73	3306.5
74	2526.0
75	1791.0
76	1174.5
77	857.5
78	636.0
79	424.0
80	258.0
81	157.0
82	95.0
83	61.0
84	47.0
85	37.0
86	28.0
87	19.5
88	15.0
89	13.5
90	11.0
91	6.0
92	4.5
93	6.5
94	8.0
95	5.0
96	2.5
97	1.0
98	0.5
99	1.5
100	4.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.32928902205065064
2	0.1182953096343576
3	0.06655195781629751
4	0.041128589315417234
5	0.04480181775638628
6	0.010007378272246388
7	0.007288610764757485
8	0.015531682462995114
9	0.018597526673567708
10-11	0.021301832651761457
12-13	0.01612460516409642
14-15	0.01817814232400825
16-17	0.019508603019162393
18-19	0.019378449255506008
20-21	0.02483044579977897
22-23	0.01674645092378803
24-25	0.025539060735241502
26-27	0.02007260266167339
28-29	0.0193495261969157
30-31	0.019595372194933316
32-33	0.02409290780572613
34-35	0.01517014423061627
36-37	0.009703686157048159
38-39	0.01318891471718021
40-41	0.009356609453964469
42-43	0.008358763932598861
44-45	0.009067378868061395
46-47	0.008561225342731014
48-49	0.004526458669383121
50-51	0.00657999582929495
52-53	0.007780302760792712
54-55	0.003398459384361129
56-57	0.0028055366832598254
58-59	0.0029646135055065165
60-61	0.003383997855065975
62-63	0.0026753829196034416
64-65	0.0021403063356827535
66-67	0.0018221526911893712
68-69	0.00297907503480167
70-71	0.0024439984508809816
72-73	0.001981229513436062
74-75	0.002082460218502138
76-77	0.003600920794493281
78-79	0.003268305620704745
80-81	0.003528613148017512
82-83	0.002328306216519752
84-85	0.0030947672691629003
86-87	0.002371690804405213
88-89	0.595251007317823
90-91	0.6058946928790562
92-93	0.602395002789629
94-95	0.5888300883107749
96-97	0.5865307051528453
98-99	0.5842891681120965
100	0.5855473211607749
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3457449.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.8817140081341
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.15936028130427	40.323887342497564
2	15.163507923848071	16.947256263211067
3	5.452077885077059	9.140143713718468
4	2.5505253078874532	5.701109033034993
5	1.3479026957422429	3.7661556477130516
6	0.8095898037939447	2.7144759527708775
7	0.551609195190746	2.15774071029344
8	0.3568538317275184	1.5953283013843196
9	0.2633947369672498	1.3247054426206406
>10	1.262755676906881	12.356605827254265
>50	0.06336966631322945	2.3843085701923736
>100	0.01894926128370956	1.5548354784070393
>500	1.0373395768273805E-4	0.033447716901857286
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	2.892305859030748E-5	0.0
12-13	0.0	0.0	0.0	2.892305859030748E-5	0.0
14-15	0.0	0.0	0.0	2.892305859030748E-5	0.0
16-17	0.0	0.0	0.0	4.338458788546122E-5	0.0
18-19	0.0	0.0	0.0	1.590768222466911E-4	0.0
20-21	0.0	0.0	0.0	1.7353835154184486E-4	0.0
22-23	0.0	0.0	0.0	2.6030752731276733E-4	0.0
24-25	0.0	0.0	0.0	4.772304667400734E-4	0.0
26-27	0.0	0.0	0.0	6.65230347577072E-4	0.0
28-29	0.0	0.0	0.0	0.0015184605759911425	0.0
30-31	0.0	0.0	0.0	0.0054086119563874985	0.0
32-33	0.0	0.0	0.0	0.013622760596034823	0.0
34-35	0.0	0.0	0.0	0.025727060616078504	0.0
36-37	0.0	0.0	0.0	0.04765073902753157	0.0
38-39	0.0	0.0	0.0	0.08448425414228815	0.0
40-41	0.0	0.0	0.0	0.12727591932664806	0.0
42-43	0.0	0.0	0.0	0.1707183533292899	0.0
44-45	0.0	0.0	0.0	0.2223315513836936	0.0
46-47	0.0	0.0	0.0	0.28610689557532154	0.0
48-49	0.0	0.0	0.0	0.3583422344046145	0.0
50-51	0.0	0.0	0.0	0.4345544937900747	0.0
52-53	0.0	0.0	0.0	0.5125455212788388	0.0
54-55	0.0	0.0	0.0	0.5994593123427128	0.0
56-57	0.0	0.0	0.0	0.7001549408248683	0.0
58-59	0.0	0.0	0.0	0.8177271739944681	0.0
60-61	0.0	0.0	0.0	0.9383941744332309	0.0
62-63	0.0	0.0	0.0	1.0628934801352095	0.0
64-65	0.0	0.0	0.0	1.1974290871680249	0.0
66-67	0.0	0.0	0.0	1.3521529890968744	0.0
68-69	0.0	0.0	0.0	1.5292054922574418	0.0
70-71	0.0	0.0	0.0	1.7172921422702114	0.0
72-73	0.0	0.0	0.0	1.9148366324420114	0.0
74-75	0.0	0.0	0.0	2.1178620422166747	0.0
76-77	0.0	0.0	0.0	2.336231134573496	0.0
78-79	0.0	0.0	0.0	2.5836389777549864	0.0
80-81	0.0	2.892305859030748E-5	0.0	2.8533320375803086	0.0
82-83	0.0	2.892305859030748E-5	0.0	3.138354318458494	0.0
84-85	0.0	2.892305859030748E-5	0.0	3.416623065155842	0.0
86-87	0.0	2.892305859030748E-5	0.0	3.7149644145148635	0.0
88	0.0	2.892305859030748E-5	0.0	3.9579181066734463	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2800	0.0	35.65187	1
GTATCAA	4310	0.0	33.86795	1
TCAACGC	4425	0.0	31.416878	4
CAACGCA	4600	0.0	30.220797	5
ATCAACG	4710	0.0	29.619856	3
AACGCAG	4890	0.0	28.520065	6
GTACATG	10415	0.0	26.8554	1
TACATGG	10420	0.0	24.899773	2
TATCAAC	5730	0.0	24.60881	2
ACATGGG	10975	0.0	22.897955	3
ACGCAGA	6020	0.0	22.698616	7
CGCAGAG	6155	0.0	21.819618	8
GTACTAG	700	0.0	19.507627	1
CATGGGG	7490	0.0	19.062346	4
GAGTACA	7515	0.0	18.296091	1
CTAGTAC	710	0.0	17.201345	3
ACCGTCC	360	2.226443E-9	16.95704	8
GCAGAGT	7700	0.0	16.770699	9
TATACCG	200	3.0116687E-4	16.437664	5
AGTACAT	7870	0.0	16.424116	2
>>END_MODULE