FastQCFastQC Report
Wed 25 May 2016
SRR1033114_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033114_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3457449
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53700.15531682462995117No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43480.12575745875065691No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA45850.052.34641
GTATCAA82700.041.0285071
TCAACGC98650.033.7420044
ATCAACG98950.033.687223
CAACGCA100650.033.1182375
AACGCAG105000.031.8676156
TATCAAC107450.031.0660952
GTACATG102850.026.904751
ACGCAGA123800.026.8764217
CGCAGAG125200.026.573588
TACATGG102100.025.5565362
ACATGGG106550.023.3419423
GCAGAGT140850.023.3206949
GAGTACT88850.020.67922212-13
CATGGGG73050.020.402054
CAGAGTA137250.020.0289610-11
AGAGTAC128500.019.94832810-11
TATACTG17200.018.8605945
GTACTTT97850.018.56109214-15
GTGGTAT24100.018.5507951
Produced by FastQC (version 0.11.5)