FastQCFastQC Report
Wed 25 May 2016
SRR1033113_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033113_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3371426
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC33950.034.455944
ATCAACG35150.033.148443
GTATCAA37250.033.135941
CAACGCA35250.033.050975
GGTATCA24350.032.310361
AACGCAG39250.030.0315346
GTACATG84950.027.6178761
TACATGG84650.026.3776132
TATCAAC44850.026.2027242
ACATGGG84900.025.2344533
ACGCAGA46350.024.9246377
CGCAGAG46850.024.3579168
CATGGGG57550.019.836524
GAGTACA59500.019.1612011
GCAGAGT61250.017.86469
CTGTCGC6900.017.6956989
TATACCG2403.4378609E-617.6166325
GTATTAG17350.016.5635991
CCTACAC11700.016.4639783
AGAGTAC50350.015.80933810-11
Produced by FastQC (version 0.11.5)