##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033113_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3371426
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.55167071737597	33.0	31.0	34.0	30.0	34.0
2	31.751686971625656	34.0	31.0	34.0	30.0	34.0
3	31.83596080708875	34.0	31.0	34.0	30.0	34.0
4	35.235425010069925	37.0	35.0	37.0	33.0	37.0
5	35.31845456492297	37.0	35.0	37.0	33.0	37.0
6	35.399381448680764	37.0	36.0	37.0	35.0	37.0
7	35.316724139874346	37.0	36.0	37.0	33.0	37.0
8	35.3065127337809	37.0	36.0	37.0	33.0	37.0
9	36.98510867508289	39.0	38.0	39.0	34.0	39.0
10-11	36.971234723823095	39.0	38.0	39.0	34.0	39.0
12-13	36.92309767439653	39.0	38.0	39.0	34.0	39.0
14-15	38.38168567247212	41.0	39.0	41.0	34.0	41.0
16-17	38.393933012321796	41.0	39.0	41.0	34.0	41.0
18-19	38.38688258321553	41.0	39.0	41.0	34.0	41.0
20-21	38.41116533478712	41.0	39.0	41.0	34.0	41.0
22-23	38.377799631372596	41.0	39.0	41.0	34.0	41.0
24-25	38.37537365494601	41.0	39.0	41.0	34.0	41.0
26-27	38.3374469734765	40.0	39.0	41.0	34.0	41.0
28-29	38.31008199497779	40.0	39.0	41.0	34.0	41.0
30-31	38.30968928281386	40.0	39.0	41.0	34.0	41.0
32-33	38.38051346818824	41.0	39.0	41.0	34.0	41.0
34-35	38.35223656102789	41.0	39.0	41.0	34.0	41.0
36-37	38.27857737349122	41.0	39.0	41.0	33.5	41.0
38-39	38.201412844297934	41.0	39.0	41.0	33.0	41.0
40-41	38.14137860359385	41.0	38.0	41.0	33.0	41.0
42-43	38.0292917596293	40.0	38.0	41.0	33.0	41.0
44-45	37.909129252725705	40.0	38.0	41.0	33.0	41.0
46-47	37.78719820633762	40.0	38.0	41.0	33.0	41.0
48-49	37.67278712331221	40.0	38.0	41.0	32.5	41.0
50-51	37.55627648360071	40.0	38.0	41.0	32.0	41.0
52-53	37.41565824075629	40.0	37.0	41.0	32.0	41.0
54-55	37.27249181800224	40.0	37.0	41.0	31.0	41.0
56-57	37.12172742335142	40.0	37.0	41.0	31.0	41.0
58-59	36.93248598664185	40.0	36.0	41.0	31.0	41.0
60-61	36.719868981256	39.5	36.0	41.0	31.0	41.0
62-63	36.464050820038764	39.0	35.0	41.0	31.0	41.0
64-65	36.20018754675321	39.0	35.0	41.0	30.0	41.0
66-67	35.91751591166468	38.5	35.0	40.5	30.0	41.0
68-69	35.617108754574474	37.5	35.0	40.0	30.0	41.0
70-71	35.27538554902287	37.0	35.0	40.0	29.5	41.0
72-73	34.91215927622318	37.0	34.5	39.0	29.0	41.0
74-75	34.53786958396833	36.0	34.0	39.0	29.0	41.0
76-77	34.20718161988428	36.0	34.0	39.0	29.0	40.0
78-79	33.83257930027234	35.0	34.0	37.5	29.0	39.5
80-81	33.46611923263332	35.0	34.0	37.0	28.0	39.0
82-83	33.23016892555257	35.0	34.0	37.0	28.0	39.0
84-85	32.950093817868165	35.0	34.0	36.0	28.0	38.0
86-87	32.6846936578172	35.0	34.0	36.0	28.0	37.0
88-89	32.30971241842472	35.0	34.0	35.5	27.0	37.0
90-91	32.108575125184416	35.0	34.0	35.0	26.5	36.5
92-93	31.926011278313688	35.0	33.0	35.0	26.0	36.0
94-95	31.750539979225408	35.0	33.0	35.0	26.0	36.0
96-97	31.56404975224134	35.0	33.0	35.0	25.0	36.0
98-99	31.364504515300055	35.0	33.0	35.0	25.0	35.5
100	31.19600637830995	35.0	33.0	35.0	24.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.019830983372777666
1101	2	0.03280345381340055
1101	3	0.032715579475716794
1101	4	-0.06967436728345433
1101	5	0.043535634475588836
1101	6	0.008903801339755546
1101	7	-0.05643947535934046
1101	8	-0.13557616729892885
1101	9	0.009543016631866408
1101	10-11	-0.0023982820847479047
1101	12-13	-0.029110253959771626
1101	14-15	-0.012434469652127689
1101	16-17	-0.1368703663549553
1101	18-19	-0.01829688065421209
1101	20-21	-0.07773868578795629
1101	22-23	-0.07676183616913335
1101	24-25	-0.012786317821252169
1101	26-27	-0.05586328020665832
1101	28-29	-0.019975305948761957
1101	30-31	0.011822517395870591
1101	32-33	0.04177665894162885
1101	34-35	0.0032609772114895463
1101	36-37	-0.03645286855526564
1101	38-39	-0.03650533756312768
1101	40-41	0.04311817562917497
1101	42-43	0.03378494534477028
1101	44-45	-0.10790169481144574
1101	46-47	-0.09921025436821651
1101	48-49	-0.05384784243476304
1101	50-51	-0.0832780653767955
1101	52-53	-0.0812774127928435
1101	54-55	-0.11304769194256892
1101	56-57	-0.05909420957961942
1101	58-59	-0.10984941548900196
1101	60-61	-0.13190736165125827
1101	62-63	-0.09434593569253735
1101	64-65	-0.0979177794267585
1101	66-67	-0.09495778134248667
1101	68-69	-0.011279177370866478
1101	70-71	-0.022889826178285944
1101	72-73	0.017056741598374003
1101	74-75	-0.06831541955033771
1101	76-77	-0.11030975975236856
1101	78-79	-0.128360019223571
1101	80-81	-0.13924334191033694
1101	82-83	-0.1409152667424749
1101	84-85	-0.13163054698915744
1101	86-87	-0.0766211074217722
1101	88-89	0.09492310188962705
1101	90-91	0.040115563574296687
1101	92-93	0.10774225453889841
1101	94-95	0.09083797697125817
1101	96-97	0.05692873143080135
1101	98-99	0.06345429793036317
1101	100	0.09746851249763466
1102	1	0.18131693287457296
1102	2	0.21254273273149948
1102	3	0.2538507531692993
1102	4	0.2559320001335337
1102	5	0.15577434802448664
1102	6	0.164582281956541
1102	7	0.14507219844124108
1102	8	0.19820076914427176
1102	9	0.28209744594175135
1102	10-11	0.2897847909044131
1102	12-13	0.26410423763376656
1102	14-15	0.3299578615082268
1102	16-17	0.29294237924965216
1102	18-19	0.3310371018994971
1102	20-21	0.282849118375303
1102	22-23	0.2892916434636206
1102	24-25	0.2984696686513928
1102	26-27	0.3703385945289952
1102	28-29	0.35554672126481535
1102	30-31	0.3447895311443574
1102	32-33	0.3227391660176906
1102	34-35	0.311351723620497
1102	36-37	0.30254002371614064
1102	38-39	0.2155713431399633
1102	40-41	0.2733268988465909
1102	42-43	0.23475216055076942
1102	44-45	0.23625007962484545
1102	46-47	0.231766024636471
1102	48-49	0.23075031514150623
1102	50-51	0.23916297251940222
1102	52-53	0.15500348302392553
1102	54-55	0.23314813370043908
1102	56-57	0.16478691850370097
1102	58-59	0.17598499520664035
1102	60-61	0.18315979025485518
1102	62-63	0.20214474519805492
1102	64-65	0.23697005830083384
1102	66-67	0.20338768178434918
1102	68-69	0.14562128794945295
1102	70-71	0.2454091487500989
1102	72-73	0.22665686275913544
1102	74-75	0.19828373529426813
1102	76-77	0.18992166142837164
1102	78-79	0.1441870298712331
1102	80-81	0.0764883961276368
1102	82-83	0.11391720863712607
1102	84-85	0.17134027584488365
1102	86-87	0.2110066508106101
1102	88-89	0.2622444532723094
1102	90-91	0.2603869837316424
1102	92-93	0.30952578862601143
1102	94-95	0.24931346964757495
1102	96-97	0.21869141663394842
1102	98-99	0.2906681745954245
1102	100	0.30834957062545953
1103	1	0.009893204374129994
1103	2	0.019659868832714977
1103	3	0.029902145528403423
1103	4	-0.005176325982169772
1103	5	-0.04998125192409475
1103	6	-0.008872109616731905
1103	7	0.05287381035073224
1103	8	-0.04371553348987334
1103	9	-0.011102444147091717
1103	10-11	-0.01090383290202368
1103	12-13	0.01898601278015377
1103	14-15	-0.0027883351313917615
1103	16-17	-0.05667151593124942
1103	18-19	-0.03957515989382898
1103	20-21	-0.0523717195790212
1103	22-23	-0.034539162756153985
1103	24-25	-0.07958699809670122
1103	26-27	0.03253652336143631
1103	28-29	0.03713546239787746
1103	30-31	0.017136005790504782
1103	32-33	0.037753089220245784
1103	34-35	0.05241347820558673
1103	36-37	0.006857917144266423
1103	38-39	-0.022499860161929064
1103	40-41	-0.02012072730283876
1103	42-43	-0.03787577368312611
1103	44-45	-0.0839875501684304
1103	46-47	-0.062381862159170964
1103	48-49	-0.09334292426031254
1103	50-51	-0.12141898946386931
1103	52-53	-0.16092709165899066
1103	54-55	-0.0685269021963677
1103	56-57	-0.14391256451155954
1103	58-59	-0.18873283603173263
1103	60-61	-0.12038534104417664
1103	62-63	-0.03947554316594193
1103	64-65	-0.06889827569131768
1103	66-67	-0.06991034191250378
1103	68-69	-0.09810448715860787
1103	70-71	-0.08277824252681398
1103	72-73	-0.10309226493109236
1103	74-75	-0.04842857690522351
1103	76-77	-0.1163005904710559
1103	78-79	-0.18795226845323043
1103	80-81	-0.16680334989126067
1103	82-83	-0.22141546556304093
1103	84-85	-0.22761569762208467
1103	86-87	-0.2327503572991816
1103	88-89	-0.20506714150047145
1103	90-91	-0.20046469740755413
1103	92-93	-0.23871830030396524
1103	94-95	-0.2952571590206041
1103	96-97	-0.24720912498843006
1103	98-99	-0.1577651079403921
1103	100	-0.0966275178355005
1104	1	-0.08631912914307094
1104	2	-0.09332798230986228
1104	3	-0.05737213025747323
1104	4	-0.13285364173337655
1104	5	-0.14510431515198263
1104	6	-0.09949585419870033
1104	7	-0.14278829478567445
1104	8	-0.20704338119186616
1104	9	-0.0993590216558573
1104	10-11	-0.1610295479379289
1104	12-13	-0.16594473440898128
1104	14-15	-0.2667546001168475
1104	16-17	-0.16820958616021642
1104	18-19	-0.22826341656262628
1104	20-21	-0.2439646209491073
1104	22-23	-0.29496363958732985
1104	24-25	-0.2509994785981746
1104	26-27	-0.17982153686111957
1104	28-29	-0.15068988822292795
1104	30-31	-0.13073243598452677
1104	32-33	-0.16078235426238052
1104	34-35	-0.23713325307485178
1104	36-37	-0.19462515763191135
1104	38-39	-0.23561777894033753
1104	40-41	-0.22604610954948612
1104	42-43	-0.21615681454620272
1104	44-45	-0.26210714235346444
1104	46-47	-0.31733567273386143
1104	48-49	-0.4048850151464194
1104	50-51	-0.30477134865186883
1104	52-53	-0.34256025686052993
1104	54-55	-0.37501161635503166
1104	56-57	-0.44097877523266504
1104	58-59	-0.3951665056867384
1104	60-61	-0.3328187107888638
1104	62-63	-0.3661382923543641
1104	64-65	-0.40910960209406966
1104	66-67	-0.4226570523801243
1104	68-69	-0.46326013223175266
1104	70-71	-0.34023459059672234
1104	72-73	-0.28607023413659505
1104	74-75	-0.33654513264352204
1104	76-77	-0.39407399536351306
1104	78-79	-0.38415375988881806
1104	80-81	-0.4132879719357945
1104	82-83	-0.33232722197279685
1104	84-85	-0.3541441178590077
1104	86-87	-0.32035626468597656
1104	88-89	-0.2048898633002949
1104	90-91	-0.20721979557175274
1104	92-93	-0.29648989444845597
1104	94-95	-0.28363822752621104
1104	96-97	-0.28322738731933583
1104	98-99	-0.21639108135227403
1104	100	-0.154407597267749
1105	1	-0.010652822601812773
1105	2	0.020280669133359908
1105	3	-0.048994542601491275
1105	4	0.0011275047112633274
1105	5	-0.030296532354242345
1105	6	-0.008281729509484137
1105	7	-0.055418897480358
1105	8	-0.07371914150423464
1105	9	-0.044020926510555114
1105	10-11	-0.07337594092845023
1105	12-13	-0.04877488645707473
1105	14-15	-0.09246041849545605
1105	16-17	-0.05330417178866753
1105	18-19	0.012451281697110517
1105	20-21	0.027962791632894834
1105	22-23	0.12588963800643427
1105	24-25	0.09141759086694634
1105	26-27	0.07774892318683868
1105	28-29	0.12655317422517953
1105	30-31	0.1018204823250386
1105	32-33	0.10130315678080137
1105	34-35	0.06280911035514691
1105	36-37	0.14085132517806898
1105	38-39	0.13106702576935447
1105	40-41	0.0494488685538812
1105	42-43	0.05470645381228678
1105	44-45	0.0806854792391718
1105	46-47	0.08466971972350734
1105	48-49	0.05681082412732508
1105	50-51	0.12236668698010078
1105	52-53	0.08515689971831364
1105	54-55	-0.041142081367226524
1105	56-57	0.03170223330588584
1105	58-59	0.06255708619044498
1105	60-61	0.008614955165320737
1105	62-63	0.09284728245083329
1105	64-65	-0.008725053486564605
1105	66-67	-4.1251487666471576E-4
1105	68-69	-0.11292927500018379
1105	70-71	-0.0962475375416858
1105	72-73	-0.10125592100445857
1105	74-75	-0.1120080859934447
1105	76-77	-0.06989450388785201
1105	78-79	-0.08960814641753245
1105	80-81	-0.1348084425415479
1105	82-83	-0.11502287804260192
1105	84-85	-0.1602400256864911
1105	86-87	-0.09120617608682124
1105	88-89	-0.07630596595328853
1105	90-91	-0.03485414699941458
1105	92-93	-0.01294961545455564
1105	94-95	-0.028025709792807163
1105	96-97	0.0054749065794119645
1105	98-99	0.03824781599313809
1105	100	0.0462418583433859
1106	1	0.03661603172701078
1106	2	0.05108019690313981
1106	3	0.024238425527855156
1106	4	0.09274785304668853
1106	5	0.020335489238661353
1106	6	0.05181432085930027
1106	7	0.02039724749322147
1106	8	0.09114684470070955
1106	9	0.04249290594541577
1106	10-11	0.06353206927816757
1106	12-13	0.021285552019726595
1106	14-15	0.0537570649216903
1106	16-17	0.09295481265991867
1106	18-19	0.12047321927589394
1106	20-21	0.08011773889166562
1106	22-23	0.10159802531336481
1106	24-25	0.11067294367951774
1106	26-27	0.1346077404559125
1106	28-29	0.12524366880604276
1106	30-31	0.11664806624222024
1106	32-33	0.12168544863605746
1106	34-35	0.09125691301004935
1106	36-37	0.10203355008213322
1106	38-39	0.051382545804187885
1106	40-41	0.09072382908688326
1106	42-43	0.12018701018789812
1106	44-45	0.10642625070727973
1106	46-47	0.04112811596031918
1106	48-49	0.08449832788627987
1106	50-51	0.07091168562306649
1106	52-53	0.06646825064538575
1106	54-55	0.061759773788025996
1106	56-57	0.07021225201741998
1106	58-59	0.03126872397263725
1106	60-61	0.03655322055882948
1106	62-63	0.048437801485818
1106	64-65	0.08760105387161587
1106	66-67	0.13614896648767427
1106	68-69	0.034342124725235124
1106	70-71	0.04509574036738684
1106	72-73	0.11961491526860613
1106	74-75	0.14235289963297504
1106	76-77	0.08412513527795795
1106	78-79	0.09652176619789543
1106	80-81	0.13248836688205756
1106	82-83	0.008838047440711705
1106	84-85	0.10470687131096668
1106	86-87	0.11117181965797585
1106	88-89	0.1529654614013296
1106	90-91	0.18196155193825092
1106	92-93	0.20105250436088795
1106	94-95	0.12790265244331067
1106	96-97	0.10649091670421384
1106	98-99	0.15194967059886366
1106	100	0.2443108830878451
1107	1	0.08264934769054477
1107	2	0.0946895523697826
1107	3	0.1036173646513241
1107	4	0.09583424850157485
1107	5	0.05983226276554632
1107	6	0.03676963972939973
1107	7	0.08603978798163325
1107	8	0.004795696171925101
1107	9	0.08400503283325378
1107	10-11	0.12356654182070059
1107	12-13	0.07488295172725401
1107	14-15	0.05578931694679312
1107	16-17	0.05310833015805372
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2215	100	0.01009355815954649
2216	1	0.17762315089543534
2216	2	0.1770056829356932
2216	3	0.2130829258035014
2216	4	0.15944219850938168
2216	5	0.17268843175401116
2216	6	0.19363756101930818
2216	7	0.23310103547405703
2216	8	0.30381911209898504
2216	9	0.22186005679444065
2216	10-11	0.27596324215490853
2216	12-13	0.24981731086892722
2216	14-15	0.24688200168223062
2216	16-17	0.2981175254247148
2216	18-19	0.25358750180363643
2216	20-21	0.23408392137597644
2216	22-23	0.23666220418528638
2216	24-25	0.28317638421663105
2216	26-27	0.20742490735145935
2216	28-29	0.27021636349789446
2216	30-31	0.23996536201350693
2216	32-33	0.2769299217915915
2216	34-35	0.27146615312054223
2216	36-37	0.3152400292792734
2216	38-39	0.29630533520607116
2216	40-41	0.27732059110227425
2216	42-43	0.2871223432642438
2216	44-45	0.3109680355847573
2216	46-47	0.35253072808547614
2216	48-49	0.3634415337971362
2216	50-51	0.3559753506474195
2216	52-53	0.35585064931757415
2216	54-55	0.3049629153900071
2216	56-57	0.32735540574343247
2216	58-59	0.32756485550051906
2216	60-61	0.3556599079366549
2216	62-63	0.42303597440938745
2216	64-65	0.4187816590157709
2216	66-67	0.4838590651374446
2216	68-69	0.4709315756208241
2216	70-71	0.3949349267694231
2216	72-73	0.5126674926349111
2216	74-75	0.4888411618715267
2216	76-77	0.42544359089632167
2216	78-79	0.4534851043117385
2216	80-81	0.4797778519812681
2216	82-83	0.46858660837437327
2216	84-85	0.4368140057016916
2216	86-87	0.4289112586591983
2216	88-89	0.4931785757155822
2216	90-91	0.5068501108151438
2216	92-93	0.5416256740823897
2216	94-95	0.5693758623966687
2216	96-97	0.5753902924518357
2216	98-99	0.5830535917524386
2216	100	0.6539660306714197
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	35619.0
3	6336.0
4	4117.0
5	3272.0
6	2789.0
7	3063.0
8	3689.0
9	4102.0
10	4275.0
11	4877.0
12	5316.0
13	5890.0
14	6553.0
15	6897.0
16	6789.0
17	6810.0
18	6958.0
19	6840.0
20	6786.0
21	7298.0
22	8313.0
23	11322.0
24	17273.0
25	24424.0
26	25802.0
27	22068.0
28	22890.0
29	28772.0
30	35007.0
31	43054.0
32	56707.0
33	76766.0
34	108222.0
35	164246.0
36	305605.0
37	655982.0
38	1110164.0
39	515378.0
40	1155.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.69795438799488	20.036230331340576	13.290377147809277	23.975438132855263
2	15.018174367015305	20.158847531315146	41.102103118745134	23.72087498292441
3	17.884093750992438	25.657844553477165	30.18461526589365	26.273446429636742
4	11.777207061647728	16.712704764193845	39.83593642202529	31.674151752133138
5	12.397412283552049	38.48253416029634	36.40551217669494	12.714541379456671
6	30.51965445955242	38.926221282460425	17.95413736956281	12.599986888424347
7	26.77776980162923	32.97447586321868	23.057835844139202	17.18991849101289
8	24.44663707407749	37.53179057512722	20.536689282856837	17.48488306793846
9	24.58216954110368	17.11169883751804	21.34677028187629	36.95936133950199
10-11	23.38159670680316	27.360688330473153	29.432197671319056	19.825517291404633
12-13	23.91576856379634	24.695170113343632	29.450402130853725	21.938659192006302
14-15	21.213699839562548	26.766310152178246	26.30927997759572	25.710710030663492
16-17	21.13696307781811	30.188950446213553	26.91976848486161	21.75431799110673
18-19	21.005278620563214	29.26725579074436	28.717959443651846	21.00950614504058
20-21	22.141213531255026	28.51545973952613	28.189051529300492	21.154275199918356
22-23	21.71912592298131	28.32119007852572	28.279628107877198	21.680055890615776
24-25	21.63157295123649	28.356544870132684	28.263504889642597	21.748377288988234
26-27	21.393675763273144	28.450877212500135	28.228420127493408	21.927026896733306
28-29	21.443708890221586	28.419089785395148	28.35929644104259	21.77790488334068
30-31	21.660187623517036	28.843774489247835	27.985995357991918	21.51004252924321
32-33	21.333383769494873	28.441352015480938	28.55990666343282	21.66535755159137
34-35	21.471579198745737	28.297510829814865	28.53139478225787	21.699515189181533
36-37	21.790814105516237	28.5866080506542	28.00724097603373	21.615336867795826
38-39	21.56128182685349	28.725691788483054	28.21998932349873	21.493037061164724
40-41	21.38954413476496	28.483389912562252	28.438508206855715	21.68855774581707
42-43	21.3780858457046	28.356290109097337	28.455470855662725	21.81015318953534
44-45	21.454298382405597	28.545419811890245	28.39790194170063	21.602379864003534
46-47	21.483265915256567	28.746901816409547	28.21369757931209	21.556134689021793
48-49	21.491444098432353	28.5269540153939	28.261534712665366	21.72006717350838
50-51	21.48440153452913	28.446958157592096	28.267644993751478	21.8009953141273
52-53	21.43947906420453	28.47212416838813	28.366255690912695	21.722141076494648
54-55	21.60735059700687	28.792240898070133	28.156777260406763	21.44363124451624
56-57	21.466178471457397	28.533168966897033	28.200851948890186	21.799800612755384
58-59	21.28849961973487	28.240410615151518	28.54509730871952	21.925992456394088
60-61	21.612076850256553	28.584530729083163	28.162573029856798	21.640819390803482
62-63	21.43301858193987	28.617895921441278	28.139376710374336	21.80970878624451
64-65	21.402064454891956	28.372165453008442	28.29783321221469	21.927936879884914
66-67	21.259259215316263	28.377906198991447	28.25353543113469	22.109299154557597
68-69	21.60076717986503	28.499883650517297	28.23790821872279	21.661440950894885
70-71	21.581116184083392	28.291954045149538	28.284701762868163	21.842228007898907
72-73	21.44817870548243	28.427428594140768	28.286254929176618	21.838137771200188
74-75	21.438289872516982	28.445732882752345	28.21330380610028	21.902673438630387
76-77	21.469121451205623	28.4333203512807	28.130026775854333	21.967531421659345
78-79	21.616254017520998	28.508555905220096	28.169119781189984	21.706070296068923
80-81	21.601181732094748	28.251316805200332	28.32818574906435	21.819315713640567
82-83	21.385064097411334	28.35655299222597	28.41547592316794	21.842906987194755
84-85	21.338203414168312	28.313618335296702	28.303029053637086	22.045149196897903
86-87	21.582556483485973	28.40751429355766	28.148197694017686	21.86173152893868
88-89	21.40633881933783	28.297742189120196	28.24656733074624	22.049351660795732
90-91	21.357810540389107	28.258252141792923	28.310372895417537	22.073564422400434
92-93	21.48792342612867	28.36202491038156	28.18958449125451	21.96046717223526
94-95	21.34576916696206	28.100320339531258	28.350346895248734	22.203563598257944
96-97	21.37928976736588	28.113557854189498	28.308649167576082	22.19850321086854
98-99	21.364319686287566	28.22009577800722	28.23917619158227	22.17640834412294
100	21.51270082096221	28.100627917489046	28.123124937529	22.263546324019746
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	624.0
1	554.0
2	470.0
3	395.5
4	324.5
5	282.0
6	260.0
7	259.5
8	242.0
9	242.5
10	252.5
11	258.0
12	284.5
13	306.0
14	322.5
15	405.5
16	536.5
17	773.0
18	1114.5
19	1574.5
20	2438.5
21	3723.5
22	5819.0
23	9284.5
24	13563.0
25	18692.5
26	25997.0
27	35326.0
28	46003.5
29	56057.5
30	64810.0
31	73682.0
32	82212.5
33	90853.0
34	99373.0
35	106207.0
36	111373.0
37	115283.0
38	118530.0
39	120480.0
40	121584.0
41	122181.5
42	120684.5
43	118300.0
44	116278.0
45	113806.0
46	111473.5
47	108310.5
48	105139.5
49	103068.0
50	100107.5
51	96106.0
52	89792.5
53	84662.5
54	81938.5
55	77380.5
56	69967.0
57	63165.0
58	57711.5
59	51420.5
60	44914.5
61	38614.5
62	33168.5
63	28060.5
64	23612.0
65	19475.5
66	15306.0
67	11552.5
68	8645.0
69	6794.0
70	5128.5
71	3729.0
72	2790.0
73	2164.5
74	1672.5
75	1226.5
76	796.0
77	547.0
78	414.0
79	274.5
80	198.5
81	118.0
82	68.0
83	46.5
84	24.0
85	17.5
86	16.0
87	15.5
88	12.5
89	9.0
90	6.5
91	7.0
92	7.0
93	7.0
94	5.0
95	3.0
96	4.5
97	4.5
98	3.5
99	3.0
100	5.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.33392398350134334
2	0.12000856610822838
3	0.06534327017707048
4	0.04125850604462325
5	0.0446398645558289
6	0.010648313206340581
7	0.007830514447002543
8	0.015927978250152904
9	0.018419505574199167
10-11	0.021341117972039133
12-13	0.016921623075814213
14-15	0.019042387405210734
16-17	0.02000637119130006
18-19	0.019546625077934383
20-21	0.02372883165758347
22-23	0.01649153800202051
24-25	0.024677984923886807
26-27	0.01968009975600829
28-29	0.01926484520200058
30-31	0.020362303666163813
32-33	0.024574171285384878
34-35	0.015527554215931182
36-37	0.009891956697255108
38-39	0.013985180158188257
40-41	0.010144075533616933
42-43	0.009135600188169635
44-45	0.009165261227741615
46-47	0.008824159272663851
48-49	0.004641952693014766
50-51	0.0067923780619832674
52-53	0.007771192367858586
54-55	0.003203392273773768
56-57	0.0028771208384819956
58-59	0.0027881377197660575
60-61	0.003455511110135592
62-63	0.0026101714823341814
64-65	0.002254239007470429
66-67	0.0016313571764588635
68-69	0.0034851721497075718
70-71	0.0021800864085404813
72-73	0.001379238340097039
74-75	0.00222457796789845
76-77	0.00311440915505783
78-79	0.002951273437411944
80-81	0.003069917595699861
82-83	0.0021652558887544915
84-85	0.0027436461604080885
86-87	0.0019131370523926671
88-89	0.5982928292063951
90-91	0.6096381768426773
92-93	0.6054856313026001
94-95	0.592686892727291
96-97	0.5908034167144703
98-99	0.5879262958759883
100	0.5893945173348013
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3371426.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.819531952860174
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.3171206647679	43.85795842297788
2	15.045387600559105	18.00016088629623
3	5.311108058316975	9.531239945987563
4	2.383602892524132	5.70344037569109
5	1.2832476428660762	3.838163668792988
6	0.7270150031507506	2.6093818326711036
7	0.4539857698142973	1.9010051384485125
8	0.299913988669307	1.4352571542650683
9	0.22815424109961135	1.2283271924074886
>10	0.8978011432586678	8.99563471482213
>50	0.03926390405373052	1.6071628328470848
>100	0.013250396299133101	1.2298556892961383
>500	1.4869462041486897E-4	0.06241214549681618
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	2.9661039571979336E-5	0.0
18-19	0.0	0.0	0.0	8.8983118715938E-5	0.0
20-21	0.0	0.0	0.0	8.8983118715938E-5	0.0
22-23	0.0	0.0	0.0	1.3347467807390702E-4	0.0
24-25	0.0	0.0	0.0	2.0762727700385534E-4	0.0
26-27	0.0	0.0	0.0	3.262714352917727E-4	0.0
28-29	0.0	0.0	0.0	0.001156780543307194	0.0
30-31	0.0	0.0	0.0	0.004597461133656797	0.0
32-33	0.0	0.0	0.0	0.012650433377449186	0.0
34-35	0.0	0.0	0.0	0.023877136855443362	0.0
36-37	0.0	0.0	0.0	0.04496613599112067	0.0
38-39	0.0	0.0	0.0	0.07977336592883841	0.0
40-41	0.0	0.0	0.0	0.12190687264083505	0.0
42-43	0.0	0.0	0.0	0.16580521120736447	0.0
44-45	0.0	0.0	0.0	0.21446414662519658	0.0
46-47	0.0	0.0	0.0	0.2781019070268782	0.0
48-49	0.0	0.0	0.0	0.34847272341139923	0.0
50-51	0.0	0.0	0.0	0.42240286454455767	0.0
52-53	0.0	0.0	0.0	0.4978902102552451	0.0
54-55	0.0	0.0	0.0	0.5832250211038297	0.0
56-57	0.0	0.0	0.0	0.6825153510710305	0.0
58-59	0.0	0.0	0.0	0.793803571545097	0.0
60-61	0.0	0.0	0.0	0.9137676460939673	0.0
62-63	0.0	0.0	0.0	1.0367423161593936	0.0
64-65	0.0	0.0	0.0	1.16526360062478	0.0
66-67	0.0	0.0	0.0	1.3097128633403194	0.0
68-69	0.0	0.0	0.0	1.476749007689921	0.0
70-71	0.0	0.0	0.0	1.6626644037270877	0.0
72-73	0.0	0.0	0.0	1.8537704816893505	0.0
74-75	0.0	0.0	0.0	2.04947402078527	0.0
76-77	0.0	0.0	0.0	2.257679688060779	0.0
78-79	0.0	0.0	0.0	2.4962137682986367	0.0
80-81	0.0	0.0	0.0	2.75828388343686	0.0
82-83	0.0	0.0	0.0	3.029593412401755	0.0
84-85	0.0	0.0	0.0	3.2972101419399387	0.0
86-87	0.0	0.0	0.0	3.5837209536854733	0.0
88	0.0	0.0	0.0	3.8182063020217556	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCAACGC	3395	0.0	34.45594	4
ATCAACG	3515	0.0	33.14844	3
GTATCAA	3725	0.0	33.13594	1
CAACGCA	3525	0.0	33.05097	5
GGTATCA	2435	0.0	32.31036	1
AACGCAG	3925	0.0	30.031534	6
GTACATG	8495	0.0	27.617876	1
TACATGG	8465	0.0	26.377613	2
TATCAAC	4485	0.0	26.202724	2
ACATGGG	8490	0.0	25.234453	3
ACGCAGA	4635	0.0	24.924637	7
CGCAGAG	4685	0.0	24.357916	8
CATGGGG	5755	0.0	19.83652	4
GAGTACA	5950	0.0	19.161201	1
GCAGAGT	6125	0.0	17.8646	9
CTGTCGC	690	0.0	17.695698	9
TATACCG	240	3.4378609E-6	17.616632	5
GTATTAG	1735	0.0	16.563599	1
CCTACAC	1170	0.0	16.463978	3
AGAGTAC	5035	0.0	15.809338	10-11
>>END_MODULE