FastQCFastQC Report
Wed 25 May 2016
SRR1033113_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033113_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3371426
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64680.19184760395156233No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52750.156461983742191No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA45950.050.893931
GTATCAA84900.042.7862971
ATCAACG97700.036.5199173
TCAACGC98150.036.3524784
CAACGCA99250.035.9022145
AACGCAG104250.034.3043676
TATCAAC106100.033.8949552
ACGCAGA121000.029.3225967
CGCAGAG121800.029.0909888
GTACATG88300.026.2712861
GCAGAGT137800.025.3721989
TACATGG90000.024.6018282
ACATGGG90850.023.1294423
GAGTACT93950.021.68336712-13
AGAGTAC125500.021.24988210-11
GTGGTAT21150.020.4678651
CAGAGTA138850.020.23902910-11
GAGTACA64400.019.4352841
AGTACTT102750.019.11740112-13
GTACTTT107100.018.99909214-15
Produced by FastQC (version 0.11.5)