FastQCFastQC Report
Wed 25 May 2016
SRR1033112_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033112_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1352036
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG652.7966216E-643.359025
GGTATCA9750.034.78891
GTATCAA16150.033.2540971
ATCAACG15500.032.1277243
TCAACGC15400.031.7215334
CAACGCA15750.031.016615
AACGCAG16700.028.9655636
GTACATG36650.027.2505131
CTGTCGC3500.026.8363329
TATCAAC19400.026.407272
TACATGG37150.026.314992
ATACCGT1501.1706652E-625.0472436
ACATGGG38100.024.5377043
TAACCGT1151.3846482E-424.5027397
ACGCAGA20600.023.0258337
CGCAGAG20600.023.0258338
GTCTCGC4500.021.9846521
CATGGGG25900.021.9445724
CTAACCG1303.176644E-421.6754996
CGCAACG550.003073074421.34866130-31
Produced by FastQC (version 0.11.5)