##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1033112_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1352036 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.387739675570767 33.0 31.0 34.0 30.0 34.0 2 31.530443715995727 33.0 31.0 34.0 30.0 34.0 3 31.705488611249997 34.0 31.0 34.0 30.0 34.0 4 35.04560603415885 37.0 35.0 37.0 33.0 37.0 5 35.15876722217456 37.0 35.0 37.0 33.0 37.0 6 35.2113183376774 37.0 36.0 37.0 33.0 37.0 7 35.15344857681304 37.0 36.0 37.0 33.0 37.0 8 35.1314883627359 37.0 35.0 37.0 33.0 37.0 9 36.774907620803 39.0 38.0 39.0 33.0 39.0 10-11 36.7633879571254 39.0 38.0 39.0 33.0 39.0 12-13 36.70409108929052 39.0 38.0 39.0 33.0 39.0 14-15 38.139189341112214 41.0 39.0 41.0 33.0 41.0 16-17 38.146833368342264 41.0 39.0 41.0 33.0 41.0 18-19 38.14065010103281 41.0 39.0 41.0 33.5 41.0 20-21 38.167467064486445 41.0 39.0 41.0 34.0 41.0 22-23 38.13194138321761 40.5 39.0 41.0 33.0 41.0 24-25 38.12590234283702 40.5 39.0 41.0 33.0 41.0 26-27 38.0857111053256 40.0 38.5 41.0 33.0 41.0 28-29 38.049960947785415 40.0 38.0 41.0 33.0 41.0 30-31 38.051192054057736 40.0 38.0 41.0 33.0 41.0 32-33 38.1156792422687 41.0 39.0 41.0 33.0 41.0 34-35 38.08745514172699 41.0 38.5 41.0 33.0 41.0 36-37 38.00458826540122 41.0 38.0 41.0 33.0 41.0 38-39 37.92375018120819 40.5 38.0 41.0 33.0 41.0 40-41 37.858486756269805 40.0 38.0 41.0 33.0 41.0 42-43 37.74536698726957 40.0 38.0 41.0 32.0 41.0 44-45 37.62106519353035 40.0 38.0 41.0 32.0 41.0 46-47 37.497248593972344 40.0 38.0 41.0 32.0 41.0 48-49 37.3722578392883 40.0 38.0 41.0 31.0 41.0 50-51 37.247887630211025 40.0 37.0 41.0 31.0 41.0 52-53 37.096508894733574 40.0 37.0 41.0 31.0 41.0 54-55 36.941496750086536 40.0 37.0 41.0 30.5 41.0 56-57 36.78721609483771 40.0 36.0 41.0 30.0 41.0 58-59 36.588683659310846 40.0 36.0 41.0 30.0 41.0 60-61 36.36898536725353 39.0 35.0 41.0 30.0 41.0 62-63 36.10270029792106 39.0 35.0 41.0 30.0 41.0 64-65 35.828852929951566 39.0 35.0 41.0 29.5 41.0 66-67 35.54467151762231 38.0 35.0 40.0 29.0 41.0 68-69 35.241143357129545 37.5 35.0 40.0 29.0 41.0 70-71 34.889734444940814 37.0 34.5 40.0 29.0 41.0 72-73 34.53432711850868 36.5 34.0 39.0 28.5 41.0 74-75 34.17029650098074 36.0 34.0 39.0 28.0 41.0 76-77 33.84367021292333 35.5 34.0 38.5 28.0 40.0 78-79 33.477675890286946 35.0 34.0 37.0 27.5 39.5 80-81 33.117085639731485 35.0 34.0 37.0 27.0 39.0 82-83 32.89648944258881 35.0 34.0 36.5 27.0 39.0 84-85 32.61965029037688 35.0 34.0 36.0 27.0 37.5 86-87 32.35197620477561 35.0 33.5 36.0 26.0 37.0 88-89 31.98763161631791 35.0 33.0 35.5 26.0 37.0 90-91 31.79475065752687 35.0 33.0 35.0 25.0 36.0 92-93 31.60181570609067 35.0 33.0 35.0 25.0 36.0 94-95 31.422985038859913 35.0 33.0 35.0 25.0 36.0 96-97 31.23248160551938 35.0 33.0 35.0 24.0 36.0 98-99 31.033151853944716 35.0 33.0 35.0 23.0 35.5 100 30.868152919005116 35.0 33.0 35.0 20.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.17439846554746552 1101 2 0.14706341536943057 1101 3 0.12554381360916267 1101 4 0.10651472839604281 1101 5 0.19579349830253534 1101 6 0.17848501797104177 1101 7 0.27251357176725577 1101 8 0.08033600897815774 1101 9 0.19774198590044278 1101 10-11 0.20858449797090373 1101 12-13 0.19384031720540662 1101 14-15 0.24980921789755683 1101 16-17 0.142738743691055 1101 18-19 0.1919889082508206 1101 20-21 0.08174325666480797 1101 22-23 0.08016005884053357 1101 24-25 0.08484444030711558 1101 26-27 0.049498473019674805 1101 28-29 0.15538070351791333 1101 30-31 0.16761058268205886 1101 32-33 0.23410312993114246 1101 34-35 0.20735864317602193 1101 36-37 0.24649220246943315 1101 38-39 0.217670253331967 1101 40-41 0.3461364721468243 1101 42-43 0.2878925032127242 1101 44-45 0.25984354869496684 1101 46-47 0.3005890351784757 1101 48-49 0.1832035939105694 1101 50-51 0.1389785590762358 1101 52-53 0.14407898296748556 1101 54-55 0.15317709350517106 1101 56-57 0.16347309969884805 1101 58-59 0.20374913176536324 1101 60-61 0.20227562940704047 1101 62-63 0.18455230646905108 1101 64-65 0.1778646138842106 1101 66-67 0.18305303301579556 1101 68-69 0.12198117880875259 1101 70-71 0.13352714427149692 1101 72-73 0.2081824548245308 1101 74-75 0.2341894207424744 1101 76-77 0.13375537122765735 1101 78-79 0.06931757267084748 1101 80-81 0.08676618854819651 1101 82-83 0.11038080442391873 1101 84-85 0.020840144285209306 1101 86-87 0.03895195227814696 1101 88-89 0.08454399314772942 1101 90-91 0.1167996301892984 1101 92-93 0.20152555035719644 1101 94-95 0.24706480136461195 1101 96-97 0.2819919351533322 1101 98-99 0.29083460216726564 1101 100 0.25395782415667867 1102 1 0.30835321116140335 1102 2 0.2912898268604138 1102 3 0.20426117752831985 1102 4 0.2329398081843479 1102 5 0.14098826502196715 1102 6 0.15902413842047736 1102 7 0.14879817271553009 1102 8 0.11494705704212294 1102 9 0.26963948470376664 1102 10-11 0.2079695740375911 1102 12-13 0.2689020483209461 1102 14-15 0.3059965915737237 1102 16-17 0.2792424304189609 1102 18-19 0.25867038793677466 1102 20-21 0.27234776251594894 1102 22-23 0.23366945898670366 1102 24-25 0.2912239547583866 1102 26-27 0.3897558522905129 1102 28-29 0.30280697202042006 1102 30-31 0.27702994905209266 1102 32-33 0.24471243471084847 1102 34-35 0.2526664050757148 1102 36-37 0.3383834822530858 1102 38-39 0.19270737769645052 1102 40-41 0.2848226919989685 1102 42-43 0.2715083244967147 1102 44-45 0.11260250414451178 1102 46-47 0.15406660569639996 1102 48-49 0.15037010816228502 1102 50-51 0.1463789476425461 1102 52-53 0.04407261704184151 1102 54-55 0.09763414737926723 1102 56-57 0.1009919067243743 1102 58-59 0.0491471184189578 1102 60-61 -0.0679351439427478 1102 62-63 0.10635278611706411 1102 64-65 0.1306398514128091 1102 66-67 0.10482253998710434 1102 68-69 0.1132818965668747 1102 70-71 0.14469909894121713 1102 72-73 0.12902633172221556 1102 74-75 0.11440853100642556 1102 76-77 0.17120109252988414 1102 78-79 0.16292936628263988 1102 80-81 0.14440438116396592 1102 82-83 0.11629695294096365 1102 84-85 0.19242828358634512 1102 86-87 0.18153203745913515 1102 88-89 0.22496235688448607 1102 90-91 0.29203507762206016 1102 92-93 0.31014281009553457 1102 94-95 0.35549206270667355 1102 96-97 0.26359636877570125 1102 98-99 0.2805787286826913 1102 100 0.33277410476668123 1103 1 -0.05853202808906133 1103 2 -0.02018075891808735 1103 3 0.0802005722632444 1103 4 0.09489851288224571 1103 5 0.1284326125440245 1103 6 0.04847477940099054 1103 7 0.16137148601735163 1103 8 0.08357164878473355 1103 9 0.17658388771422295 1103 10-11 0.15264417932414887 1103 12-13 0.12999227007168912 1103 14-15 0.12557671741902965 1103 16-17 -0.014292753402770586 1103 18-19 0.054616990197125403 1103 20-21 -0.037874990591689084 1103 22-23 -0.07569984674005781 1103 24-25 -0.031351351352618906 1103 26-27 0.022197353794666697 1103 28-29 0.04476264573908395 1103 30-31 -0.06227290185833567 1103 32-33 -0.013578188465643848 1103 34-35 0.016011723107773435 1103 36-37 0.09128397425022428 1103 38-39 -0.014300389486159304 1103 40-41 -0.018989182041167396 1103 42-43 -0.09221180872317802 1103 44-45 -0.1932031337155209 1103 46-47 -0.1446514332838973 1103 48-49 -0.06304006566556808 1103 50-51 -0.16017655925293184 1103 52-53 -0.21637119468901034 1103 54-55 -0.19372422056967764 1103 56-57 -0.18821911983486928 1103 58-59 -0.1695941182921672 1103 60-61 -0.15840056631685684 1103 62-63 -0.08754706985175886 1103 64-65 -0.054899633058191455 1103 66-67 -0.06128108847689617 1103 68-69 -0.09342432398101153 1103 70-71 -0.08137925545184999 1103 72-73 0.06087965619332181 1103 74-75 0.20114262701687124 1103 76-77 0.07846685093696948 1103 78-79 -0.1288368712339647 1103 80-81 -0.0556872641923718 1103 82-83 0.001799087156065582 1103 84-85 -0.07082264450188092 1103 86-87 0.01039829609712939 1103 88-89 0.1227572598275124 1103 90-91 0.028292987702212002 1103 92-93 0.079931822915146 1103 94-95 0.05053688898025044 1103 96-97 0.14651322090090702 1103 98-99 0.10698535960607813 1103 100 0.23654886721070056 1104 1 0.21783517751359938 1104 2 0.13475368454183112 1104 3 0.1963912673683339 1104 4 0.2401788220073371 1104 5 0.19565633524347703 1104 6 0.17576457767999898 1104 7 0.21555619548500715 1104 8 0.22218067858872104 1104 9 0.27034141842096915 1104 10-11 0.2863368161929998 1104 12-13 0.30564009622312227 1104 14-15 0.26966636387899 1104 16-17 0.30327182066972114 1104 18-19 0.2676179328098982 1104 20-21 0.22938562098511284 1104 22-23 0.2758534715905654 1104 24-25 0.21841392231094403 1104 26-27 0.27908910903789064 1104 28-29 0.3367484212767593 1104 30-31 0.34388151609450546 1104 32-33 0.3448328231423048 1104 34-35 0.2828411148305747 1104 36-37 0.33920943832713135 1104 38-39 0.276120852760684 1104 40-41 0.35340635199484893 1104 42-43 0.2878512592079687 1104 44-45 0.25759331382997885 1104 46-47 0.1727790942085079 1104 48-49 0.16717584085542114 1104 50-51 0.15133749744842362 1104 52-53 0.1008477778723389 1104 54-55 0.16083350218167425 1104 56-57 0.1663223829327194 1104 58-59 0.2070335570783044 1104 60-61 0.1250839681603395 1104 62-63 0.17707026365706469 1104 64-65 0.1226469739065763 1104 66-67 0.17464900246623216 1104 68-69 0.2060261197977411 1104 70-71 0.2588408126535384 1104 72-73 0.28960691577608344 1104 74-75 0.23939294029427316 1104 76-77 0.23795864640575815 1104 78-79 0.2763786413731353 1104 80-81 0.26383204963743623 1104 82-83 0.16181386124413422 1104 84-85 0.17233935791536226 1104 86-87 0.1993491736445847 1104 88-89 0.2956752627118924 1104 90-91 0.2710776437829381 1104 92-93 0.23409975628530333 1104 94-95 0.22834382668438735 1104 96-97 0.24468084933537426 1104 98-99 0.22494391708413985 1104 100 0.23234881827269405 1105 1 -0.06135016542227589 1105 2 0.00852473806328291 1105 3 0.04039555541575979 1105 4 -0.09626517436652904 1105 5 -0.09314878523195347 1105 6 -0.04514720797518379 1105 7 -0.15976891549340166 1105 8 -0.13024951473315127 1105 9 -0.16871129002777963 1105 10-11 -0.08777351289305102 1105 12-13 -0.02465602007217882 1105 14-15 -0.02482640004097192 1105 16-17 -0.04066681564485464 1105 18-19 0.019907874244950108 1105 20-21 0.006960697788642278 1105 22-23 0.020059031662796656 1105 24-25 0.0506022474543002 1105 26-27 0.13968012752109615 1105 28-29 0.059016634686251734 1105 30-31 0.09034801392529346 1105 32-33 0.07757698463478135 1105 34-35 0.09377221828331983 1105 36-37 0.10549241708115886 1105 38-39 0.06448866974164247 1105 40-41 0.048936098525196314 1105 42-43 0.05890860284892341 1105 44-45 0.09602260061833334 1105 46-47 0.14153404057194052 1105 48-49 0.06734496151791092 1105 50-51 0.07732641039962118 1105 52-53 0.04243145055618669 1105 54-55 0.023659152763364943 1105 56-57 0.16755177908066798 1105 58-59 0.04907301466907654 1105 60-61 -0.046598011248406124 1105 62-63 -0.03472604869409679 1105 64-65 0.025005534892535763 1105 66-67 0.10321946746037014 1105 68-69 -0.05042776030960283 1105 70-71 0.055492889046973914 1105 72-73 0.03806814707883177 1105 74-75 0.034088055445614884 1105 76-77 0.06918917572060224 1105 78-79 0.0702600797396471 1105 80-81 0.12056647848534396 1105 82-83 0.11757628471040249 1105 84-85 0.038241421718055335 1105 86-87 0.15608040983973837 1105 88-89 0.08801793238114541 1105 90-91 0.07850986961051376 1105 92-93 0.13971819560871523 1105 94-95 0.19272236558258982 1105 96-97 0.34717601988598545 1105 98-99 0.2835868777794346 1105 100 0.27576979517047917 1106 1 0.10022452263963899 1106 2 0.07926304702830222 1106 3 0.08394141078833783 1106 4 0.08344759252461387 1106 5 0.018329689485526046 1106 6 0.1133340104654934 1106 7 0.028899212198886914 1106 8 0.03969747641116328 1106 9 -0.017939488322092245 1106 10-11 0.0297343387597806 1106 12-13 0.02238134545958559 1106 14-15 0.029646936094444243 1106 16-17 0.007118888150358771 1106 18-19 0.04277414541442681 1106 20-21 0.028989429920372345 1106 22-23 0.04738567232855928 1106 24-25 0.010619902371452383 1106 26-27 0.02573501433425207 1106 28-29 0.1592850308850018 1106 30-31 0.11656929270935734 1106 32-33 0.14955021161226512 1106 34-35 0.12888027581462325 1106 36-37 0.004244025231081139 1106 38-39 0.06543867452695196 1106 40-41 0.1025780702129353 1106 42-43 0.11471190157956102 1106 44-45 0.11428538411470868 1106 46-47 0.08597728440165042 1106 48-49 0.1011777456802676 1106 50-51 0.06871605481821064 1106 52-53 0.035086334634868876 1106 54-55 0.03856571577924228 1106 56-57 0.05650740009667743 1106 58-59 0.041589852555659945 1106 60-61 0.004998737741722437 1106 62-63 0.09113558227094387 1106 64-65 0.07788615757348083 1106 66-67 -0.0232922596298053 1106 68-69 -0.10078429878149109 1106 70-71 -0.09213064355066791 1106 72-73 -0.025903252220004447 1106 74-75 -0.07634327495361504 1106 76-77 -0.08271522221937033 1106 78-79 0.059958658304914536 1106 80-81 0.033406736988965235 1106 82-83 0.020921567345645542 1106 84-85 0.11322002014785681 1106 86-87 0.05405258625483356 1106 88-89 0.028719374633073613 1106 90-91 0.02387125293039105 1106 92-93 0.01310546986680805 1106 94-95 -0.06569955474137856 1106 96-97 -0.21256898277570713 1106 98-99 -0.18944802585506082 1106 100 -0.18128438729836205 1107 1 0.22694790707568657 1107 2 0.2581658875509092 1107 3 0.2958997701978383 1107 4 0.27104361020568035 1107 5 0.2603580565567114 1107 6 0.2178047559639822 1107 7 0.1481725236362763 1107 8 0.1237998648639973 1107 9 0.14757712243522292 1107 10-11 0.13813624492591714 1107 12-13 0.19489181503237063 1107 14-15 0.2224686400045357 1107 16-17 0.15890883534309808 1107 18-19 0.20865772297565854 1107 20-21 0.09321201946268332 1107 22-23 0.1068995306703826 1107 24-25 0.007746663033664447 1107 26-27 0.12590291781902607 1107 28-29 0.111274765024298 1107 30-31 0.07367880034011876 1107 32-33 0.10936959421336923 1107 34-35 0.05957387486978405 1107 36-37 0.022505420498738715 1107 38-39 0.06562462484050258 1107 40-41 0.08188188802724028 1107 42-43 0.10839375725843325 1107 44-45 0.06515196081767982 1107 46-47 0.07926607883402426 1107 48-49 0.07204998860505185 1107 50-51 0.0496693045411547 1107 52-53 0.03523500717329853 1107 54-55 0.0511588060585666 1107 56-57 0.07334303010642884 1107 58-59 -0.005375539196478485 1107 60-61 0.03545906563702772 1107 62-63 0.0020935596928879363 1107 64-65 0.032036796624190345 1107 66-67 0.030827504651860238 1107 68-69 -0.07718012121473095 1107 70-71 -0.08540551363762461 1107 72-73 -0.1009035043931874 1107 74-75 -0.10766143023879238 1107 76-77 -0.10934679368811118 1107 78-79 -0.10687736641069279 1107 80-81 -0.08618529942570774 1107 82-83 -0.03642601369953269 1107 84-85 -0.09925463644708543 1107 86-87 -0.1113173254933244 1107 88-89 -0.03526079973043039 1107 90-91 -0.18775544392104138 1107 92-93 -0.19133012488255474 1107 94-95 -0.2726000875504262 1107 96-97 -0.27866280698862766 1107 98-99 -0.3075567357414357 1107 100 -0.25018139055994837 1108 1 -0.13550968332620172 1108 2 -0.16378614010819348 1108 3 -0.16481060764712296 1108 4 -0.23938406589796557 1108 5 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0.06530189986888502 2214 36-37 0.0661720780914763 2214 38-39 0.0698649577585897 2214 40-41 0.13266887843436592 2214 42-43 0.10533022636840172 2214 44-45 0.21021641379162048 2214 46-47 0.20681558251176568 2214 48-49 0.15128936011888072 2214 50-51 0.14934683442785968 2214 52-53 0.20088431368755977 2214 54-55 0.03711236569565557 2214 56-57 0.024284947029869386 2214 58-59 0.14696447557241754 2214 60-61 0.25663929847402045 2214 62-63 0.2757062965494157 2214 64-65 0.24762046839342844 2214 66-67 0.28288102095014267 2214 68-69 0.24870362534959867 2214 70-71 0.2955678854946484 2214 72-73 0.2535061869074724 2214 74-75 0.3068831255557072 2214 76-77 0.3215596744237317 2214 78-79 0.2136237045288496 2214 80-81 0.24106018445877453 2214 82-83 0.16940037699874466 2214 84-85 0.1938208115348985 2214 86-87 0.1154911191360597 2214 88-89 0.03188183766288333 2214 90-91 -0.07847024475048414 2214 92-93 -0.08601887403295194 2214 94-95 -0.014704690062696812 2214 96-97 0.08778679836031955 2214 98-99 -0.00904114392473332 2214 100 0.09716014301164222 2215 1 -0.17298610589219976 2215 2 -0.20239968825767107 2215 3 -0.15710120526263438 2215 4 -0.28486844533819067 2215 5 -7.306338397867762E-4 2215 6 0.09340963709168904 2215 7 0.112223936141298 2215 8 0.07958377167883413 2215 9 0.05997694646980278 2215 10-11 -0.06523530988157233 2215 12-13 -0.030975101556201423 2215 14-15 -0.055719298713590604 2215 16-17 0.08009736698818415 2215 18-19 -0.0027446449068264656 2215 20-21 -0.05588520143129472 2215 22-23 0.0737386561987563 2215 24-25 0.05754296393453018 2215 26-27 -0.008882767776675848 2215 28-29 0.11570498134700102 2215 30-31 0.07506309351381191 2215 32-33 0.024420853704981482 2215 34-35 0.016727729851325535 2215 36-37 -0.10605692290160817 2215 38-39 0.050899475174261966 2215 40-41 0.02120011766210439 2215 42-43 -0.07380456724474982 2215 44-45 0.007090545061124942 2215 46-47 -0.023312675254302917 2215 48-49 0.06500180850827064 2215 50-51 0.052987782822810914 2215 52-53 -0.09432431421223697 2215 54-55 -0.025534382932121957 2215 56-57 -0.05521453162491241 2215 58-59 -0.04781046068147532 2215 60-61 -0.1305987351777631 2215 62-63 0.06860896623039281 2215 64-65 -0.14142302779293203 2215 66-67 -0.049009631702212175 2215 68-69 0.06301363201289689 2215 70-71 -0.04708999999074592 2215 72-73 0.028280455976343433 2215 74-75 0.015002724457765737 2215 76-77 -0.16213641937905976 2215 78-79 -2.557111881529295E-4 2215 80-81 -0.08340236807135426 2215 82-83 -0.1530557021259753 2215 84-85 -0.1259578669426631 2215 86-87 -0.2040506231235284 2215 88-89 -0.15647849955637483 2215 90-91 -0.07683745910762241 2215 92-93 -0.11536614041341409 2215 94-95 -0.19495295559548964 2215 96-97 -0.24281941621151049 2215 98-99 -0.35181108227854807 2215 100 -0.23346467575781205 2216 1 0.028306657430640314 2216 2 -0.03187927525605616 2216 3 0.049633822900965185 2216 4 0.08585588215305506 2216 5 0.08450307169466953 2216 6 0.18767192496299856 2216 7 0.09051117758642846 2216 8 0.12856833382129196 2216 9 0.07957094726680936 2216 10-11 0.0654117051639389 2216 12-13 -0.002536375749052411 2216 14-15 -0.02227617880431154 2216 16-17 0.07867775090691964 2216 18-19 -0.026630702627471692 2216 20-21 0.06272234762737838 2216 22-23 0.06715366089196095 2216 24-25 0.066541856392071 2216 26-27 -0.020300615660694632 2216 28-29 0.06781839492658293 2216 30-31 -0.021219975513616873 2216 32-33 -0.035351361142424764 2216 34-35 0.03457079750641867 2216 36-37 -0.010825535376987716 2216 38-39 0.04079996789430851 2216 40-41 0.03143245966136732 2216 42-43 0.020350947023544563 2216 44-45 0.05836309727352784 2216 46-47 0.15362262104189028 2216 48-49 0.180371401321473 2216 50-51 0.16021575095302865 2216 52-53 0.25389200580859494 2216 54-55 0.29903075930219813 2216 56-57 0.15805040062497966 2216 58-59 0.09046355447223675 2216 60-61 0.1170685200082886 2216 62-63 0.1229138447833904 2216 64-65 0.11738496973687518 2216 66-67 0.13266856783312875 2216 68-69 0.07203011847299479 2216 70-71 0.10509394354658497 2216 72-73 0.12750040388050365 2216 74-75 0.12757289153920937 2216 76-77 0.07600302364760125 2216 78-79 0.12574241856938073 2216 80-81 0.2139246191052564 2216 82-83 0.08301534597514859 2216 84-85 0.06006913017456128 2216 86-87 -0.002006046078953716 2216 88-89 0.1712919400561681 2216 90-91 0.23429983041312852 2216 92-93 0.19456024188139054 2216 94-95 0.21907669997551693 2216 96-97 0.19595983271811335 2216 98-99 0.026968659283149066 2216 100 0.037028598494860177 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 17943.0 3 3072.0 4 1971.0 5 1650.0 6 1416.0 7 1464.0 8 1550.0 9 1845.0 10 1912.0 11 2286.0 12 2448.0 13 2685.0 14 2906.0 15 3128.0 16 3031.0 17 3019.0 18 3065.0 19 3045.0 20 3143.0 21 3315.0 22 3675.0 23 4865.0 24 7702.0 25 10286.0 26 11028.0 27 9663.0 28 9888.0 29 12220.0 30 15018.0 31 18265.0 32 23512.0 33 32191.0 34 44853.0 35 67683.0 36 126366.0 37 265948.0 38 428153.0 39 195328.0 40 498.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.00858006365112 19.930783677473308 13.307965887513088 23.752670371362488 2 15.49724945370426 20.04399985486416 40.73392824217544 23.72482244925614 3 18.247647383164775 25.26305272156044 29.716575818466424 26.772724076808363 4 11.98776753884243 16.785523490439413 39.34395176864577 31.882757202072387 5 12.637029659452391 38.03029507022504 35.80215488504492 13.530520385277642 6 30.736832450392846 38.44445167694586 17.813527703521356 13.005188169139936 7 27.223713894744627 32.45741336587892 22.619475572321463 17.699397167054993 8 24.623659413803768 37.16121949884379 20.443486404395806 17.771634682956634 9 24.483150181795317 17.44280752628912 21.354347367759164 36.7196949241564 10-11 23.509379065300397 27.24591375469249 28.999256894989905 20.245450285017206 12-13 24.131590948683833 24.48951436409507 29.076238020365796 22.3026566668553 14-15 21.51934738746003 26.778788982817325 25.887901109448713 25.813962520273932 16-17 21.24226667228889 30.05890755811416 26.484025865174708 22.214799904422247 18-19 21.114537845773267 28.978154135001837 28.43842493170162 21.468883087523274 20-21 22.381603579736773 28.330692869341608 27.91270674130247 21.374996809619155 22-23 21.926876629741276 28.27566437963494 27.9255450964437 21.87191389418009 24-25 21.804788009262342 28.256652684377563 27.858749287938984 22.07981001842111 26-27 21.59367144605105 28.1310244729534 28.07598584974752 22.19931823124803 28-29 21.588984022571687 28.2123152993604 28.12350660091642 22.075194077151494 30-31 21.92451279456385 28.747219839535777 27.663821868511956 21.664445497388414 32-33 21.467553739258065 28.411303591297553 28.193468727066122 21.92767394237826 34-35 21.631163178455424 28.05260093842536 28.3049238851668 22.01131199795242 36-37 22.001988281859667 28.527073697353618 27.69144794703241 21.77949007375431 38-39 21.825524514579445 28.617232882771926 27.907770649375053 21.64947195327358 40-41 21.539028254672253 28.29380184301119 28.227303727001196 21.939866175315366 42-43 21.498986451626504 28.184152548923446 28.180454142160098 22.13640685728995 44-45 21.639978815408536 28.416126326440942 28.156496731329973 21.787398126820552 46-47 21.703468211512973 28.612855934008714 27.909457252414317 21.774218602064003 48-49 21.58218120279174 28.44444625248153 27.99555173566629 21.977820809060436 50-51 21.61011480003654 28.169334954190568 28.131944780890255 22.088605464882637 52-53 21.604330326449837 28.166131453485644 28.18310688940115 22.046431330663367 54-55 21.75459864504588 28.695715204457983 27.90729152297538 21.64239462752075 56-57 21.572180627696223 28.373696151833478 27.907759387429433 22.146363833040866 58-59 21.373166982986763 28.00301921737034 28.394881383662497 22.228932415980402 60-61 21.80940490252567 28.464375290773575 27.831570632183357 21.8946491745174 62-63 21.57194352225201 28.50618139107094 27.949562186136628 21.972312900540423 64-65 21.549145919462845 28.108826158396077 28.21925437654099 22.122773545600086 66-67 21.377906310211056 28.226425002681182 27.986486836314683 22.409181850793075 68-69 21.873035315141 28.42936897835648 27.873044190893776 21.82455151560875 70-71 21.862153386439452 28.10533945754839 27.993099163467726 22.039407992544433 72-73 21.571192256870596 28.29117947591422 28.161520815466435 21.976107451748746 74-75 21.649462041899824 28.30569733001334 28.085876551994232 21.958964076092606 76-77 21.609412450402054 28.31715910507165 27.8429714869824 22.230456957543897 78-79 21.79225790561158 28.417450167289388 27.94296739234548 21.84732453475355 80-81 21.695259629576622 28.142997728909197 28.069032876267524 22.092709765246653 82-83 21.494948986912778 28.2609692560536 28.200947333073966 22.043134423959657 84-85 21.596349696042225 28.166667529587713 28.000357988960033 22.23662478541003 86-87 21.78396067776201 28.32785444766065 27.936510754185345 21.951674120391996 88-89 21.505524748836162 28.208249289643263 28.07681748277399 22.209408478746585 90-91 21.5233116129834 28.082053663179057 28.121528297099264 22.27310642673828 92-93 21.700037278255767 28.299925517896167 27.91847446365072 22.081562740197345 94-95 21.478430396641443 28.094068475320995 28.111216878194632 22.31628424984293 96-97 21.522924586553984 28.011813806083964 28.17321212031037 22.29204948705168 98-99 21.525250481322885 28.121047870425205 28.007375324725565 22.34632632352635 100 21.700011679992027 27.99902393951996 27.95222957656681 22.348734803921204 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 365.0 1 351.5 2 313.5 3 248.0 4 189.0 5 155.5 6 148.5 7 144.0 8 141.5 9 138.0 10 127.0 11 133.0 12 142.0 13 159.0 14 176.5 15 218.0 16 287.0 17 369.0 18 526.0 19 727.5 20 1072.0 21 1614.5 22 2431.5 23 3694.0 24 5260.5 25 7232.5 26 9770.0 27 12779.0 28 16465.5 29 20474.5 30 24063.5 31 27896.5 32 31777.0 33 35327.0 34 38375.5 35 40991.5 36 43386.5 37 45032.0 38 46470.5 39 47301.5 40 47398.5 41 47463.5 42 47036.5 43 46342.5 44 45599.0 45 44702.0 46 44323.5 47 43825.5 48 42640.0 49 41839.0 50 41018.0 51 39673.5 52 37422.0 53 35246.5 54 33860.0 55 32294.5 56 30143.5 57 27363.0 58 24782.5 59 22932.0 60 20737.0 61 17772.5 62 15126.0 63 12543.5 64 10120.0 65 8181.5 66 6470.5 67 4856.0 68 3604.0 69 2789.5 70 2052.0 71 1406.5 72 1064.0 73 859.0 74 665.5 75 476.5 76 335.5 77 255.0 78 163.0 79 105.5 80 77.0 81 53.5 82 35.0 83 19.0 84 13.0 85 10.5 86 9.0 87 7.5 88 5.5 89 3.5 90 2.0 91 3.0 92 4.0 93 3.0 94 1.5 95 0.5 96 1.5 97 1.5 98 1.0 99 1.0 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3237339834146428 2 0.11663890606463143 3 0.06516098683762858 4 0.04067939019375223 5 0.044007703936877425 6 0.008431728149250464 7 0.006360777375750349 8 0.01494043058025082 9 0.01782496915762598 10-11 0.02037667636068862 12-13 0.015569112065063357 14-15 0.01764006283856347 16-17 0.019008369599626044 18-19 0.018268744323376006 20-21 0.023409139993313788 22-23 0.015606093328875859 24-25 0.024111784005751327 26-27 0.01908233212725105 28-29 0.018786482016751035 30-31 0.0200068637225636 32-33 0.023963858950501318 34-35 0.014570617942125802 36-37 0.008505690676875469 38-39 0.01235174211337568 40-41 0.008727578259750481 42-43 0.007581159081562917 44-45 0.008135878038750446 46-47 0.007396252762500406 48-49 0.003550201326000196 50-51 0.0056581333633128105 52-53 0.006101908529062836 54-55 0.0025517072030626403 56-57 0.002107932037312616 58-59 0.002218875828750122 60-61 0.0028845385773751584 62-63 0.0025517072030626403 64-65 0.001960006982062608 66-67 0.0015532130801250855 68-69 0.002810576049750155 70-71 0.0019230257182501057 72-73 0.0018120819268125996 74-75 0.0018860444544376038 76-77 0.002921519841187661 78-79 0.0027735947859376525 80-81 0.0031434074240626725 82-83 0.002218875828750122 84-85 0.0028845385773751584 86-87 0.0020339695096876116 88-89 0.5911824833066576 90-91 0.6016851622294082 92-93 0.5982828859586579 94-95 0.5833794366422196 96-97 0.5803099917457819 98-99 0.5786828161380317 100 0.581123579549657 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1352036.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 76.35314574308089 #Duplication Level Percentage of deduplicated Percentage of total 1 84.84489972347808 64.78174994143804 2 10.230252313246625 15.622238917236198 3 2.468978993683961 5.655429388240699 4 0.9326347169661124 2.8483837787828232 5 0.4448631328631836 1.6983349809613104 6 0.27503693950714714 1.2599961316152073 7 0.17345164987851083 0.9270505371786243 8 0.12277951327215396 0.7499681656906643 9 0.09092488743998733 0.624816106414072 >10 0.4042683264797344 5.157769389440984 >50 0.010746165367891868 0.5276689173961665 >100 0.0010666765895291958 0.10654192126593302 >500 9.69612270747477E-5 0.04005182433925999 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 7.396252762500407E-5 7.396252762500407E-5 0.0 10-11 0.0 0.0 7.396252762500407E-5 7.396252762500407E-5 0.0 12-13 0.0 0.0 7.396252762500407E-5 7.396252762500407E-5 0.0 14-15 0.0 0.0 7.396252762500407E-5 1.1094379143750609E-4 0.0 16-17 0.0 0.0 7.396252762500407E-5 2.218875828750122E-4 0.0 18-19 0.0 0.0 7.396252762500407E-5 2.5886884668751425E-4 0.0 20-21 0.0 0.0 7.396252762500407E-5 3.6981263812502034E-4 0.0 22-23 0.0 0.0 7.396252762500407E-5 4.8075642956252644E-4 0.0 24-25 0.0 0.0 7.396252762500407E-5 8.505690676875468E-4 0.0 26-27 0.0 0.0 7.396252762500407E-5 0.001183400442000065 0.0 28-29 0.0 0.0 7.396252762500407E-5 0.0019969882458751097 0.0 30-31 0.0 0.0 7.396252762500407E-5 0.006027946001437832 0.0 32-33 0.0 0.0 7.396252762500407E-5 0.01434873035925079 0.0 34-35 0.0 0.0 7.396252762500407E-5 0.025517072030626406 0.0 36-37 0.0 0.0 7.396252762500407E-5 0.04256543464818984 0.0 38-39 0.0 0.0 7.396252762500407E-5 0.07473913416506661 0.0 40-41 0.0 0.0 7.396252762500407E-5 0.11149851039469363 0.0 42-43 0.0 0.0 7.396252762500407E-5 0.15461866400007102 0.0 44-45 0.0 0.0 7.396252762500407E-5 0.20095618755713607 0.0 46-47 0.0 0.0 7.396252762500407E-5 0.254209207447139 0.0 48-49 0.0 0.0 7.396252762500407E-5 0.31822377510658 0.0 50-51 0.0 0.0 7.396252762500407E-5 0.38993044563902146 0.0 52-53 0.0 0.0 7.396252762500407E-5 0.45882654012171276 0.0 54-55 0.0 0.0 7.396252762500407E-5 0.5335286930229668 0.0 56-57 0.0 0.0 1.4792505525000813E-4 0.6231712765044718 0.0 58-59 0.0 0.0 1.4792505525000813E-4 0.727865234357665 0.0 60-61 0.0 0.0 1.4792505525000813E-4 0.8381433630465461 0.0 62-63 0.0 0.0 1.4792505525000813E-4 0.9454260093666145 0.0 64-65 0.0 0.0 1.4792505525000813E-4 1.0600309459215582 0.0 66-67 0.0 0.0 1.4792505525000813E-4 1.1919800952045656 0.0 68-69 0.0 0.0 1.4792505525000813E-4 1.3379081622086986 0.0 70-71 0.0 0.0 1.4792505525000813E-4 1.5026227112295827 0.0 72-73 0.0 0.0 1.4792505525000813E-4 1.68157504681828 0.0 74-75 0.0 0.0 1.4792505525000813E-4 1.8608971950451023 0.0 76-77 0.0 0.0 1.4792505525000813E-4 2.0516095725261754 0.0 78-79 0.0 0.0 1.4792505525000813E-4 2.2670993967616244 0.0 80-81 0.0 0.0 1.4792505525000813E-4 2.507477611542888 0.0 82-83 0.0 0.0 1.4792505525000813E-4 2.7604294560204017 0.0 84-85 0.0 0.0 1.4792505525000813E-4 3.006835616803103 0.0 86-87 0.0 0.0 1.4792505525000813E-4 3.2635225689256795 0.0 88 0.0 0.0 1.4792505525000813E-4 3.4773482362895662 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATACCG 65 2.7966216E-6 43.35902 5 GGTATCA 975 0.0 34.7889 1 GTATCAA 1615 0.0 33.254097 1 ATCAACG 1550 0.0 32.127724 3 TCAACGC 1540 0.0 31.721533 4 CAACGCA 1575 0.0 31.01661 5 AACGCAG 1670 0.0 28.965563 6 GTACATG 3665 0.0 27.250513 1 CTGTCGC 350 0.0 26.836332 9 TATCAAC 1940 0.0 26.40727 2 TACATGG 3715 0.0 26.31499 2 ATACCGT 150 1.1706652E-6 25.047243 6 ACATGGG 3810 0.0 24.537704 3 TAACCGT 115 1.3846482E-4 24.502739 7 ACGCAGA 2060 0.0 23.025833 7 CGCAGAG 2060 0.0 23.025833 8 GTCTCGC 450 0.0 21.984652 1 CATGGGG 2590 0.0 21.944572 4 CTAACCG 130 3.176644E-4 21.675499 6 CGCAACG 55 0.0030730744 21.348661 30-31 >>END_MODULE