FastQCFastQC Report
Wed 25 May 2016
SRR1033112_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033112_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1352036
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30570.22610344694963744No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26440.19555692304051076No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14090.10421320142363073No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21450.049.3562131
GTATCAA37400.038.623651
ATCAACG45500.031.2018413
TCAACGC45700.031.0652924
CAACGCA47300.030.1138425
AACGCAG48650.029.2695456
TATCAAC49800.028.413312
CGCGATT350.00882311426.8664934-35
GTACATG36250.026.0900331
ACGCAGA55550.025.6339027
CGCAGAG55850.025.2419058
TATAACG950.00130318524.7417892
TACATGG37800.024.3752422
GTGGTAT12200.024.2978171
TGGTATC11500.023.7090872
GCAGAGT61750.022.6009889
GTACGAC1252.4204626E-422.564513
CCGGTCG1252.4271382E-422.5553329
ACATGGG39750.022.3516393
GTCTCGC3450.021.8216421
Produced by FastQC (version 0.11.5)