FastQCFastQC Report
Wed 25 May 2016
SRR1033111_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033111_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2300987
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA33650.033.0347061
GGTATCA23300.030.5256231
GTACATG76600.030.19231
TCAACGC35450.029.9483364
ATCAACG37950.028.5981123
CAACGCA37350.028.5506365
TACATGG76600.028.5393942
AACGCAG38950.027.6100336
ACATGGG81950.026.1428573
TATCAAC44100.024.6260072
CATGGGG59200.022.6947234
ACGCAGA48700.021.7930687
CGCAGAG52250.019.952888
GAGTACA53550.018.207671
TACACCG4203.6379788E-1217.8958055
CTAGTAC4500.017.7489873
TAGACTG7900.017.2445345
GCAGAGT57600.017.202799
TATACGT1650.001577220617.0823574
ACACCGT5250.016.9954726
Produced by FastQC (version 0.11.5)