##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033111_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2300987
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.355058068559273	33.0	31.0	34.0	30.0	34.0
2	31.456851777085223	33.0	31.0	34.0	29.0	34.0
3	31.62432078060415	34.0	31.0	34.0	30.0	34.0
4	35.014064399320816	37.0	35.0	37.0	33.0	37.0
5	35.079621049575685	37.0	35.0	37.0	33.0	37.0
6	35.22484481659392	37.0	35.0	37.0	33.0	37.0
7	35.108879798104034	37.0	35.0	37.0	33.0	37.0
8	35.127798201380536	37.0	35.0	37.0	33.0	37.0
9	36.775980916015605	39.0	38.0	39.0	33.0	39.0
10-11	36.762074275082824	39.0	38.0	39.0	33.0	39.0
12-13	36.715874100983626	39.0	38.0	39.0	33.0	39.0
14-15	38.137760230718385	41.0	39.0	41.0	33.0	41.0
16-17	38.130915993875675	41.0	39.0	41.0	33.0	41.0
18-19	38.09491970184968	41.0	38.5	41.0	33.5	41.0
20-21	38.13421197077602	40.5	39.0	41.0	33.5	41.0
22-23	38.08273753828249	40.0	39.0	41.0	33.0	41.0
24-25	38.07157211231528	40.0	38.5	41.0	33.0	41.0
26-27	38.038067577087574	40.0	38.0	41.0	33.0	41.0
28-29	38.01110175763705	40.0	38.0	41.0	33.0	41.0
30-31	38.003722967578696	40.0	38.0	41.0	33.0	41.0
32-33	38.07995416749421	40.5	38.5	41.0	33.0	41.0
34-35	38.045155405050096	41.0	38.0	41.0	33.0	41.0
36-37	37.95577006736674	40.5	38.0	41.0	33.0	41.0
38-39	37.86439884275748	40.0	38.0	41.0	33.0	41.0
40-41	37.788575293993404	40.0	38.0	41.0	33.0	41.0
42-43	37.65037720769392	40.0	38.0	41.0	32.0	41.0
44-45	37.48696972212359	40.0	38.0	41.0	32.0	41.0
46-47	37.336290252834985	40.0	37.5	41.0	31.0	41.0
48-49	37.20137923421558	40.0	37.0	41.0	31.0	41.0
50-51	37.050591333197445	40.0	37.0	41.0	31.0	41.0
52-53	36.885485446028156	40.0	36.5	41.0	30.5	41.0
54-55	36.71491972792545	40.0	36.0	41.0	30.0	41.0
56-57	36.53308275970268	39.5	35.5	41.0	30.0	41.0
58-59	36.31574254874104	39.0	35.0	41.0	30.0	41.0
60-61	36.07798479522049	39.0	35.0	41.0	30.0	41.0
62-63	35.79843171647645	38.5	35.0	41.0	30.0	41.0
64-65	35.52009376845675	38.0	35.0	40.0	29.0	41.0
66-67	35.21659161916169	37.0	35.0	40.0	29.0	41.0
68-69	34.90323087440303	37.0	34.0	40.0	29.0	41.0
70-71	34.5596083332935	36.5	34.0	39.0	28.5	41.0
72-73	34.19998331150937	36.0	34.0	39.0	28.0	41.0
74-75	33.84043760351536	35.0	34.0	39.0	28.0	40.0
76-77	33.521763921308555	35.0	34.0	37.5	27.5	39.5
78-79	33.17355117608227	35.0	34.0	37.0	27.0	39.0
80-81	32.833238518948605	35.0	33.0	36.5	26.5	39.0
82-83	32.640609660115416	35.0	33.0	36.0	27.0	38.5
84-85	32.392811215361064	35.0	33.0	36.0	26.5	37.0
86-87	32.15716907570534	35.0	33.0	35.5	26.0	37.0
88-89	31.819485073144698	35.0	33.0	35.0	25.0	36.5
90-91	31.64233478937517	35.0	33.0	35.0	25.0	36.0
92-93	31.479593322343845	35.0	33.0	35.0	25.0	36.0
94-95	31.309582800772016	35.0	33.0	35.0	24.0	36.0
96-97	31.131009649337436	35.0	33.0	35.0	23.5	35.5
98-99	30.9358225405011	35.0	33.0	35.0	22.0	35.0
100	30.76866796726796	35.0	33.0	35.0	20.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.008650070103865204
1101	2	0.03501725608092343
1101	3	0.0825407614120941
1101	4	0.044699976184695345
1101	5	0.059438857490277996
1101	6	0.02056946764153622
1101	7	0.11803837919665483
1101	8	0.018599040702632408
1101	9	0.06395741895509133
1101	10-11	0.11805913328093709
1101	12-13	0.0785031861507619
1101	14-15	0.1402206611127852
1101	16-17	0.06820047208729818
1101	18-19	0.0537707570469621
1101	20-21	0.13372090092202882
1101	22-23	0.103728393109769
1101	24-25	0.05531458704943759
1101	26-27	0.09294501700058788
1101	28-29	0.12556224098655377
1101	30-31	0.16637872991898917
1101	32-33	0.1984720375733815
1101	34-35	0.23869194279964745
1101	36-37	0.1583805349328813
1101	38-39	0.18511143701466892
1101	40-41	0.1698789502637723
1101	42-43	0.19098372512960538
1101	44-45	0.16557816492667854
1101	46-47	0.1586067099792956
1101	48-49	0.1500041079559793
1101	50-51	0.1261825550076736
1101	52-53	0.23122477201101077
1101	54-55	0.12157746633574362
1101	56-57	0.17078366258117228
1101	58-59	0.1393138243865053
1101	60-61	0.050482333426700166
1101	62-63	0.0741114023532532
1101	64-65	-0.0014343377460050988
1101	66-67	0.0658140982706712
1101	68-69	0.07764070338373585
1101	70-71	0.0879488841473588
1101	72-73	0.08336867393474279
1101	74-75	0.009209627814129817
1101	76-77	-0.09088769819751974
1101	78-79	-0.08234340655506145
1101	80-81	-0.08150056949513385
1101	82-83	-0.05847373184797533
1101	84-85	-0.08034369946915376
1101	86-87	-0.03256697946075349
1101	88-89	0.03753520185878756
1101	90-91	0.12248991135562903
1101	92-93	0.08739007151786993
1101	94-95	0.12255239139355467
1101	96-97	0.1583511488667817
1101	98-99	0.24490557879372332
1101	100	0.24264532876886236
1102	1	0.09027331459714105
1102	2	0.09748360010144097
1102	3	0.15479516677298122
1102	4	0.16973457227533828
1102	5	0.026525565822666408
1102	6	0.023668638087450233
1102	7	0.07214262248238157
1102	8	0.12009870602305739
1102	9	0.14381722957909915
1102	10-11	0.09106083300191159
1102	12-13	0.08052414310871114
1102	14-15	0.061742249825435636
1102	16-17	0.13089469905269624
1102	18-19	0.10620531590920024
1102	20-21	0.06748344200488532
1102	22-23	0.10997035515935494
1102	24-25	0.10291406021114113
1102	26-27	0.08483119962281904
1102	28-29	0.06950256649064102
1102	30-31	0.0482073213316454
1102	32-33	0.11837071239268937
1102	34-35	0.10138250333152143
1102	36-37	0.11142668310944259
1102	38-39	0.06647951497620852
1102	40-41	0.11325398692810751
1102	42-43	0.06850016767376843
1102	44-45	0.06221830165645059
1102	46-47	0.041480752006023636
1102	48-49	0.06533699191617615
1102	50-51	0.04789164889093911
1102	52-53	-0.010608153314073832
1102	54-55	0.09785526542270873
1102	56-57	0.03949605813483004
1102	58-59	0.05636212917806205
1102	60-61	0.023176851826555378
1102	62-63	0.1464506527462106
1102	64-65	0.13463683430937579
1102	66-67	0.06842690220860703
1102	68-69	-0.0059717959581888636
1102	70-71	0.038206427599291715
1102	72-73	0.10002264910076519
1102	74-75	0.09212691495416436
1102	76-77	-0.02021267958331663
1102	78-79	-0.021490340288501386
1102	80-81	-0.01872704415350057
1102	82-83	-0.01565307873395483
1102	84-85	-0.027110798210905784
1102	86-87	-0.05450640057790679
1102	88-89	0.11271513913457198
1102	90-91	0.07296335133517218
1102	92-93	0.0967326611856727
1102	94-95	0.06414163990629973
1102	96-97	0.06398716226900802
1102	98-99	0.04498797953678846
1102	100	0.0013742608777960186
1103	1	-0.052455491938498966
1103	2	-0.0506545718142597
1103	3	-0.0014045135490619032
1103	4	-0.09247682957168735
1103	5	-0.06541884573309886
1103	6	-0.036192538801017804
1103	7	-0.05813354375015223
1103	8	-0.10358262241643956
1103	9	-0.11199025325301193
1103	10-11	-0.06597134199216725
1103	12-13	-0.09428345812263927
1103	14-15	-0.055575119343409085
1103	16-17	-0.07833452188029355
1103	18-19	-0.07193670398186924
1103	20-21	-0.08318181373746825
1103	22-23	-0.06890426004310513
1103	24-25	-0.03606815030271093
1103	26-27	-0.035101312809096896
1103	28-29	0.01135817861472077
1103	30-31	-0.0310343315217807
1103	32-33	-0.024401013868256882
1103	34-35	-0.021561049829742274
1103	36-37	0.011331223720382866
1103	38-39	-0.05297792049620398
1103	40-41	0.0031930113183520348
1103	42-43	-0.04717589199945138
1103	44-45	-0.16022279472053214
1103	46-47	-0.12096374834917611
1103	48-49	-0.1030711302718359
1103	50-51	-0.1631779402636937
1103	52-53	-0.2594456207542848
1103	54-55	-0.14239825695474195
1103	56-57	-0.22822608844343506
1103	58-59	-0.21056008251978398
1103	60-61	-0.17660114959424078
1103	62-63	-0.15476589946784713
1103	64-65	-0.19551797269986082
1103	66-67	-0.15389357494033362
1103	68-69	-0.09169802731030785
1103	70-71	-0.05580627071943667
1103	72-73	-0.06705422160726471
1103	74-75	-0.02215647363215112
1103	76-77	-0.12756897250304178
1103	78-79	-0.17839597609243896
1103	80-81	-0.11835482653569329
1103	82-83	-0.10476700840480646
1103	84-85	-0.0975635273134472
1103	86-87	-0.07004852110833326
1103	88-89	-0.0629323567214577
1103	90-91	-0.08979194703091764
1103	92-93	-0.13106378091709558
1103	94-95	-0.06455093646120957
1103	96-97	-0.017379756982709438
1103	98-99	-0.020893058824565713
1103	100	-0.0643817182839399
1104	1	0.08710896891024333
1104	2	0.07906764158154544
1104	3	0.1402242456621039
1104	4	0.1330053395411781
1104	5	0.0058852947070775485
1104	6	0.11745587475482466
1104	7	-0.029363807079086257
1104	8	0.02830038815888969
1104	9	0.09899317169853106
1104	10-11	0.14691619689622115
1104	12-13	0.128678819054457
1104	14-15	0.08357500140197516
1104	16-17	0.19474882565555163
1104	18-19	0.13982358609592183
1104	20-21	0.11443733747734797
1104	22-23	0.04820991800723107
1104	24-25	0.09008464496741908
1104	26-27	0.12510748571653352
1104	28-29	0.22977562328993884
1104	30-31	0.1681191869929961
1104	32-33	0.12845654268420503
1104	34-35	0.0690171721655588
1104	36-37	0.1353298115337651
1104	38-39	0.02955285080105341
1104	40-41	0.06424255014608349
1104	42-43	0.05315444267903757
1104	44-45	0.029370299827725432
1104	46-47	-0.027839817148041845
1104	48-49	-0.08466350699166725
1104	50-51	-0.10772637093475623
1104	52-53	-0.08669472924604094
1104	54-55	-0.032080475246807794
1104	56-57	0.015797980834946657
1104	58-59	-0.02598546808174973
1104	60-61	-0.010433526545156724
1104	62-63	-0.10704700873665018
1104	64-65	-0.14775512039342686
1104	66-67	-0.09391748389889898
1104	68-69	-0.053480839796762325
1104	70-71	-0.164098209159512
1104	72-73	-0.13867146414950327
1104	74-75	-0.14465942005066523
1104	76-77	-0.20282301985660922
1104	78-79	-0.17426367829040856
1104	80-81	-0.1388406046501558
1104	82-83	-0.13384298664464467
1104	84-85	-0.18077019654618454
1104	86-87	-0.10278766305060572
1104	88-89	0.11698905586957764
1104	90-91	0.036827183963382026
1104	92-93	0.017084064658796194
1104	94-95	0.12118698972233943
1104	96-97	0.08089963931924515
1104	98-99	0.06827613600880156
1104	100	0.06193532356964937
1105	1	-0.040643584407725086
1105	2	0.02211937924788998
1105	3	0.007714288499471422
1105	4	-0.0770804045844784
1105	5	0.003861179230632672
1105	6	-0.05260494979959418
1105	7	-0.0772656173889601
1105	8	-0.08787991652158667
1105	9	0.01233323809075415
1105	10-11	-0.04004094166397465
1105	12-13	-0.09218334602347511
1105	14-15	-0.03410182256576633
1105	16-17	-0.04322648316833977
1105	18-19	-0.04858929302522341
1105	20-21	-0.03324697486953454
1105	22-23	-6.175950910076722E-4
1105	24-25	-0.02110472055757384
1105	26-27	-0.011113035697739804
1105	28-29	-0.03741640073386776
1105	30-31	-0.02111016618530215
1105	32-33	-0.0017360519847642308
1105	34-35	0.016345493085232476
1105	36-37	-0.0463804071267333
1105	38-39	-0.011617942412854632
1105	40-41	-0.0690248118892498
1105	42-43	-0.07890986126023591
1105	44-45	-0.06852258799403188
1105	46-47	-0.05005565603369888
1105	48-49	-0.06615274950369354
1105	50-51	-0.030089844230218432
1105	52-53	-0.001366872141552733
1105	54-55	-0.1121485620126279
1105	56-57	-0.11326368953535848
1105	58-59	-0.12826198331341487
1105	60-61	-0.10200095712473001
1105	62-63	-0.12843386580455984
1105	64-65	-0.11729264080527457
1105	66-67	-0.07568986441615522
1105	68-69	-0.07322014208475025
1105	70-71	-0.012184260496333366
1105	72-73	-0.03733654341571935
1105	74-75	-0.011122498640602885
1105	76-77	0.07561866395182903
1105	78-79	-0.01979095022017674
1105	80-81	-1.0161137239350637E-4
1105	82-83	-0.02033861453978858
1105	84-85	0.033230519480270004
1105	86-87	0.018957138711243715
1105	88-89	0.0919335698158541
1105	90-91	0.021266287575016918
1105	92-93	0.09430869195965741
1105	94-95	0.08646651718256848
1105	96-97	0.08084442526648772
1105	98-99	0.0970364036166238
1105	100	0.15410209862262647
1106	1	0.14126416825567745
1106	2	0.13897473092184143
1106	3	0.14527465679515572
1106	4	0.14140170087843984
1106	5	0.15273814897123117
1106	6	0.20845938088124427
1106	7	0.21167283312539809
1106	8	0.21276777475920028
1106	9	0.18469167525537245
1106	10-11	0.17936460240323981
1106	12-13	0.11973215308654517
1106	14-15	0.17348351922676386
1106	16-17	0.12626609594848048
1106	18-19	0.15213103653005078
1106	20-21	0.11027030453260522
1106	22-23	0.14787916890659147
1106	24-25	0.1436014216302084
1106	26-27	0.21793818410175447
1106	28-29	0.20908820950616303
1106	30-31	0.1809609244358512
1106	32-33	0.14105987536386522
1106	34-35	0.14447077938473285
1106	36-37	0.12372227623583143
1106	38-39	0.046458034931859515
1106	40-41	0.09978392040925854
1106	42-43	0.1184932386272024
1106	44-45	0.11023312981608768
1106	46-47	0.033169360653481306
1106	48-49	0.055082003002659974
1106	50-51	0.05463014667363808
1106	52-53	0.0018849176393587186
1106	54-55	0.02338533748478966
1106	56-57	0.09242704483105513
1106	58-59	0.06044333760377896
1106	60-61	0.03510174096180663
1106	62-63	0.051096578466840015
1106	64-65	0.09050227222510898
1106	66-67	0.21685747316204385
1106	68-69	0.15942454550523166
1106	70-71	0.18849398303166254
1106	72-73	0.15363614688346416
1106	74-75	0.14733409344640336
1106	76-77	0.08896484258963255
1106	78-79	0.11532368410173888
1106	80-81	0.09303501238751721
1106	82-83	0.06878081816183368
1106	84-85	0.13263837030350345
1106	86-87	0.14750995197198336
1106	88-89	0.21498440188402057
1106	90-91	0.15304234246599435
1106	92-93	0.22748376983312113
1106	94-95	0.249351590017163
1106	96-97	0.24103816005171197
1106	98-99	0.2665338498250378
1106	100	0.27780164446981104
1107	1	0.02637446237332597
1107	2	0.052739642480869264
1107	3	0.06844156341794516
1107	4	0.039083981832781944
1107	5	-0.007066201090474067
1107	6	0.05142909499066661
1107	7	-0.04133133182729409
1107	8	-0.04677305206104165
1107	9	0.0620119652594866
1107	10-11	0.06624500141599299
1107	12-13	0.0273132668257432
1107	14-15	-0.009764758231078474
1107	16-17	-0.03367322943201856
1107	18-19	-0.0043709173983756955
1107	20-21	-0.033049059926469226
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2216	3	0.2439221922289363
2216	4	0.1486619586197051
2216	5	0.18447479923754884
2216	6	0.22422995278699887
2216	7	0.1929236794736866
2216	8	0.2035499134249008
2216	9	0.12374190320517897
2216	10-11	0.18126719335747055
2216	12-13	0.19819950096953676
2216	14-15	0.19966459826441252
2216	16-17	0.15411659698666824
2216	18-19	0.17114509719628757
2216	20-21	0.10460908082582421
2216	22-23	0.1773904874043737
2216	24-25	0.14456230905459933
2216	26-27	0.07181953735624802
2216	28-29	0.1508450592849826
2216	30-31	0.20320041843242365
2216	32-33	0.19789907483123415
2216	34-35	0.21465181328380822
2216	36-37	0.24868235680818174
2216	38-39	0.2511661294068972
2216	40-41	0.28391545421805375
2216	42-43	0.2830811798567794
2216	44-45	0.40568809844477727
2216	46-47	0.46861626076181295
2216	48-49	0.4151890942728542
2216	50-51	0.4344877626797796
2216	52-53	0.48135617565145594
2216	54-55	0.3865072624193431
2216	56-57	0.3002904667864641
2216	58-59	0.2393599553703254
2216	60-61	0.34467196530052746
2216	62-63	0.3313485927757611
2216	64-65	0.3693019528696908
2216	66-67	0.38014048638764564
2216	68-69	0.3210183886035409
2216	70-71	0.3317196702664731
2216	72-73	0.39224810747748506
2216	74-75	0.39486430808032935
2216	76-77	0.3193624288043111
2216	78-79	0.34261079929537885
2216	80-81	0.36913399564110705
2216	82-83	0.3744258201930606
2216	84-85	0.3116627564188761
2216	86-87	0.32357099061060524
2216	88-89	0.36915845058853947
2216	90-91	0.3867013318132848
2216	92-93	0.3462490041023294
2216	94-95	0.31169459240950914
2216	96-97	0.3175147557003548
2216	98-99	0.3177716341980634
2216	100	0.34144195773152575
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	31039.0
3	5202.0
4	3352.0
5	2878.0
6	2422.0
7	2564.0
8	2863.0
9	3187.0
10	3614.0
11	3891.0
12	4143.0
13	4622.0
14	4971.0
15	5076.0
16	5329.0
17	5234.0
18	5082.0
19	5023.0
20	5202.0
21	5453.0
22	6368.0
23	8432.0
24	12463.0
25	16891.0
26	18298.0
27	16259.0
28	17317.0
29	22048.0
30	26881.0
31	32854.0
32	43318.0
33	58872.0
34	83511.0
35	130130.0
36	249078.0
37	492278.0
38	693014.0
39	261333.0
40	495.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.33145994175772	19.171771985718042	13.297446766393882	24.19932130613035
2	15.476254707983209	19.803335097014042	40.240420428992124	24.479989766010625
3	18.59798314006687	24.89823503011199	29.2707800589367	27.233001770884435
4	12.41361559521382	16.295245428447082	37.92335695177342	33.36778202456567
5	13.368470344342418	37.923884399175805	35.00203700169004	13.705608254791738
6	31.95268021024257	37.116210174763154	17.591928966420387	13.339180648573892
7	28.34969997626931	32.07115903995883	21.723708698384662	17.855432285387195
8	25.775405485876725	36.505703860021256	19.627668013396303	18.091222640705716
9	25.39226267657091	17.06869144639812	20.25034741495886	37.28869846207211
10-11	24.578260252497227	26.683889005892414	28.102406411126623	20.635444330483736
12-13	24.95223026836244	23.821817596995594	28.387043492623725	22.83890864201824
14-15	22.225436421768567	25.79105350859825	25.23229715605644	26.751212913576744
16-17	22.3704915011406	29.24827779864241	25.753604364880655	22.627626335336338
18-19	22.268718440908906	28.298049310478753	27.4359711642304	21.997261084381943
20-21	23.20620924017879	27.487081289795334	27.146907605819315	22.15980186420656
22-23	22.984561526130392	27.20371413569235	27.071553879482714	22.740170458694543
24-25	22.932132106023886	27.195713835486924	27.04115543866894	22.83099861982025
26-27	22.62809670917395	27.428215985154836	27.07030110664985	22.873386199021365
28-29	22.562430057623065	27.421780270799935	27.168189234378058	22.847600437198945
30-31	22.72949652264988	27.61686923950802	26.9980119802802	22.655622257561898
32-33	22.595024667138173	27.459399964093656	27.284693420227267	22.660881948540904
34-35	22.719176120248957	27.29718898369879	27.257156418663257	22.72647847738899
36-37	22.960294441708633	27.421274960762982	26.972162688779928	22.646267908748452
38-39	22.745613146716124	27.638903437721268	26.975491169788423	22.639992245774184
40-41	22.533817982043427	27.441494662880693	27.201923005876722	22.822764349199158
42-43	22.636140076982187	27.30455425783817	27.248117170087514	22.811188495092125
44-45	22.566726313131337	27.54388839606064	27.221432393115546	22.66795289769248
46-47	22.638400593184205	27.56274657152307	27.109057142223826	22.6897956930689
48-49	22.725619209006645	27.442707765162794	26.996291417323555	22.83538160850701
50-51	22.59217253566043	27.458015776449805	27.143282143935167	22.806529543954596
52-53	22.594602347429063	27.448078180992386	27.21294410893531	22.744375362643236
54-55	22.70111907751401	27.584137253342476	27.119799835978153	22.594943833165356
56-57	22.54440998236139	27.466466109077743	27.121104028673766	22.868019879887097
58-59	22.504563944221335	27.180876481557014	27.360739670796253	22.953819903425398
60-61	22.72776067820359	27.51692597077146	27.04043892351782	22.714874427507127
62-63	22.484537699936418	27.527566159434556	27.043716010863484	22.94418012976554
64-65	22.635374534891277	27.312157789390902	27.142140355215616	22.9103273205022
66-67	22.466856009161454	27.39226709026074	27.041324325440197	23.099552575137604
68-69	22.663680616657285	27.491920085061572	27.025780757839847	22.818618540441296
70-71	22.68904531750678	27.26245282912267	27.10706001843592	22.941441834934633
72-73	22.540224207733644	27.388424720001098	27.156780916027383	22.91457015623788
74-75	22.535128019362514	27.42510564156143	27.07813890056286	22.961627438513197
76-77	22.59528598017517	27.35878638925378	27.019506476263118	23.02642115430793
78-79	22.66648963576036	27.48350494248705	27.053089497163413	22.796915924589182
80-81	22.579305579096967	27.353221116862457	27.180007040692594	22.88746626334798
82-83	22.515658298262466	27.376374793480828	27.229000018036135	22.87896689022057
84-85	22.554843549232125	27.27052065652541	27.144158789603257	23.03047700463921
86-87	22.69764662422043	27.322888372194093	27.076055542276016	22.903409461309458
88-89	22.47821904264558	27.317999463577596	27.119103479466546	23.08467801431028
90-91	22.485131816109995	27.246382831278222	27.1646009883339	23.10388436427788
92-93	22.585711084757435	27.301435668184126	27.11127384810445	23.00157939895399
94-95	22.368364682985856	27.14322684884845	27.308186289852994	23.180222178312697
96-97	22.499310406329137	27.103938268361443	27.248195581442214	23.148555743867206
98-99	22.484953356881654	27.23572997720573	27.16167975113179	23.117636914780824
100	22.583207364230955	27.078140477478996	27.09304694268836	23.245605215601692
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	389.0
1	359.0
2	334.5
3	302.5
4	248.0
5	225.5
6	204.5
7	191.5
8	194.5
9	204.0
10	197.5
11	185.0
12	198.5
13	217.0
14	239.5
15	301.5
16	390.0
17	497.5
18	658.5
19	889.5
20	1328.5
21	1956.5
22	2838.5
23	4505.0
24	6590.0
25	8846.0
26	11997.0
27	16201.0
28	21349.5
29	26743.5
30	31628.0
31	36905.5
32	42622.5
33	48349.5
34	54046.0
35	58482.5
36	62237.0
37	66479.5
38	70080.0
39	72782.5
40	75410.5
41	77819.0
42	78405.0
43	78704.0
44	79355.5
45	78619.0
46	78124.0
47	77049.5
48	75826.5
49	75012.5
50	73098.5
51	72141.0
52	69942.0
53	67425.5
54	65563.0
55	62247.5
56	57499.0
57	52511.0
58	48773.0
59	45681.0
60	42620.0
61	38265.0
62	33649.5
63	29335.0
64	25282.5
65	20931.0
66	16526.0
67	12650.5
68	9597.0
69	7550.5
70	5758.0
71	4270.5
72	3251.0
73	2553.0
74	1967.0
75	1469.0
76	1071.5
77	791.0
78	603.5
79	482.0
80	346.0
81	178.5
82	110.5
83	79.0
84	53.5
85	41.0
86	34.5
87	22.5
88	18.0
89	16.0
90	11.0
91	10.5
92	8.0
93	5.5
94	4.5
95	2.0
96	2.5
97	2.5
98	2.5
99	2.0
100	2.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3243825367114199
2	0.12007890526978206
3	0.06827504892465712
4	0.04119971125434433
5	0.04511107624684538
6	0.009387275982002506
7	0.007344674263696405
8	0.015297783081781861
9	0.018426875075782695
10-11	0.021143100765019532
12-13	0.0159496772471987
14-15	0.01875282215849112
16-17	0.019252607685310695
18-19	0.019187418268769012
20-21	0.023663758204631315
22-23	0.016123515691309858
24-25	0.024424301397617632
26-27	0.01912222885222733
28-29	0.018796281769518905
30-31	0.019839312434185852
32-33	0.024250462953506472
34-35	0.015645459970004175
36-37	0.0098218720922804
38-39	0.013298640974503551
40-41	0.009300356759946928
42-43	0.008474624150418929
44-45	0.00903959909378019
46-47	0.007996568429113246
48-49	0.004693637991001253
50-51	0.006627590681737881
52-53	0.007475053096779774
54-55	0.0032160112160564136
56-57	0.0029769833554035723
58-59	0.0031073621884869407
60-61	0.0036940669373620974
62-63	0.0029552535498896778
64-65	0.002020871912792206
66-67	0.001825303663167154
68-69	0.0028466045223202044
70-71	0.0019122228852227327
72-73	0.0017601142466254697
74-75	0.0019991421072783114
76-77	0.0036723371318482024
78-79	0.003063902577459151
80-81	0.003346390049139782
82-83	0.0021947103569033635
84-85	0.0024120084120423106
86-87	0.0016080056080282068
88-89	0.5915070358937273
90-91	0.5999382004331185
92-93	0.5964614315508954
94-95	0.5847490663789061
96-97	0.5827064646606
98-99	0.5802075370265021
100	0.581880732051072
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2300987.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.27874860531405
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.5752980648607	45.55584392823416
2	13.947731381253433	16.81503587090051
3	4.553692463542771	8.234726497074238
4	2.0492143470741717	4.940963058627471
5	1.079413210498222	3.2532838778438626
6	0.6651379879171826	2.4056211336902553
7	0.44848409567178943	1.8923842039536989
8	0.31828412070143924	1.5348614797460391
9	0.22533662996402165	1.2224709062252945
>10	1.0827678667159144	11.2897972844528
>50	0.04242677940509618	1.7151901557729916
>100	0.012068875624681825	1.0903709781640134
>500	1.4417677060706043E-4	0.04945062531468
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	4.345961102778938E-5	0.0	0.0	0.0
6	0.0	4.345961102778938E-5	0.0	0.0	0.0
7	0.0	4.345961102778938E-5	0.0	0.0	0.0
8	0.0	4.345961102778938E-5	0.0	0.0	0.0
9	0.0	4.345961102778938E-5	0.0	0.0	0.0
10-11	0.0	4.345961102778938E-5	0.0	0.0	0.0
12-13	0.0	6.518941654168407E-5	0.0	0.0	0.0
14-15	0.0	8.691922205557876E-5	0.0	0.0	0.0
16-17	0.0	8.691922205557876E-5	0.0	0.0	0.0
18-19	0.0	1.3037883308336814E-4	0.0	0.0	0.0
20-21	0.0	1.3037883308336814E-4	0.0	0.0	0.0
22-23	0.0	1.3037883308336814E-4	0.0	2.172980551389469E-5	0.0
24-25	0.0	1.3037883308336814E-4	0.0	1.3037883308336814E-4	0.0
26-27	0.0	1.3037883308336814E-4	0.0	3.2594708270842034E-4	0.0
28-29	0.0	1.3037883308336814E-4	0.0	0.0010213008591530505	0.0
30-31	0.0	1.3037883308336814E-4	0.0	0.004302501491751149	0.0
32-33	0.0	1.3037883308336814E-4	0.0	0.011994852643669868	0.0
34-35	0.0	1.3037883308336814E-4	0.0	0.02218613142968648	0.0
36-37	0.0	1.3037883308336814E-4	0.0	0.04085203436612202	0.0
38-39	0.0	1.3037883308336814E-4	0.0	0.07309906574874174	0.0
40-41	0.0	1.3037883308336814E-4	0.0	0.11121314462011303	0.0
42-43	0.0	1.7383844411115752E-4	0.0	0.1489360869922342	0.0
44-45	0.0	1.7383844411115752E-4	0.0	0.19252607685310696	0.0
46-47	0.0	1.7383844411115752E-4	0.0	0.2454816128904683	0.0
48-49	0.0	1.7383844411115752E-4	0.0	0.3068031240506791	0.0
50-51	0.0	2.172980551389469E-4	0.0	0.3714058358434881	0.0
52-53	0.0	2.172980551389469E-4	0.0	0.43881169254758934	0.0
54-55	0.0	2.3902786065284158E-4	0.0	0.509237992218122	0.0
56-57	0.0	2.8248747168063096E-4	0.0	0.5950055345814644	0.0
58-59	0.0	3.2594708270842034E-4	0.0	0.6939630688917409	0.0
60-61	0.0	3.4767688822231503E-4	0.0	0.7993526256341301	0.0
62-63	0.0	3.4767688822231503E-4	0.0	0.9093706309509788	0.0
64-65	0.0	3.694066937362097E-4	0.0	1.025103575117982	0.0
66-67	0.0	4.128663047639991E-4	0.0	1.155330299562753	0.0
68-69	0.0	4.345961102778938E-4	0.0	1.2978126343173604	0.0
70-71	0.0	4.345961102778938E-4	0.0	1.4560056184585135	0.0
72-73	0.0	4.345961102778938E-4	0.0	1.6212825191971967	0.0
74-75	0.0	4.345961102778938E-4	0.0	1.7925568462577146	0.0
76-77	0.0	4.345961102778938E-4	0.0	1.9796939313433757	0.0
78-79	0.0	4.345961102778938E-4	0.0	2.191516075492821	0.0
80-81	0.0	4.345961102778938E-4	0.0	2.4199180612493683	0.0
82-83	0.0	4.345961102778938E-4	0.0	2.662053284090697	0.0
84-85	0.0	4.345961102778938E-4	0.0	2.9064266768999563	0.0
86-87	0.0	4.345961102778938E-4	0.0	3.1612086465503717	0.0
88	0.0	4.345961102778938E-4	0.0	3.3702059159830107	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	3365	0.0	33.034706	1
GGTATCA	2330	0.0	30.525623	1
GTACATG	7660	0.0	30.1923	1
TCAACGC	3545	0.0	29.948336	4
ATCAACG	3795	0.0	28.598112	3
CAACGCA	3735	0.0	28.550636	5
TACATGG	7660	0.0	28.539394	2
AACGCAG	3895	0.0	27.610033	6
ACATGGG	8195	0.0	26.142857	3
TATCAAC	4410	0.0	24.626007	2
CATGGGG	5920	0.0	22.694723	4
ACGCAGA	4870	0.0	21.793068	7
CGCAGAG	5225	0.0	19.95288	8
GAGTACA	5355	0.0	18.20767	1
TACACCG	420	3.6379788E-12	17.895805	5
CTAGTAC	450	0.0	17.748987	3
TAGACTG	790	0.0	17.244534	5
GCAGAGT	5760	0.0	17.20279	9
TATACGT	165	0.0015772206	17.082357	4
ACACCGT	525	0.0	16.995472	6
>>END_MODULE