FastQCFastQC Report
Wed 25 May 2016
SRR1033111_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033111_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2300987
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46790.2033475199990265No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38550.16753680051212805No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA35600.049.2863921
GTATCAA63600.040.309521
TCAACGC72500.034.168764
ATCAACG73400.033.7497983
CAACGCA75050.032.9451645
TATCAAC78200.031.978752
AACGCAG78500.031.6693256
ACGCAGA91300.027.0234837
GTACATG79200.026.727331
CGCAGAG94050.026.1827858
TACATGG79600.025.74722
GCAGAGT102100.023.8422579
ACATGGG82900.023.8150423
CATGGGG57050.021.50514
GAGTACT63700.021.21005412-13
CGTAGAC1605.410247E-520.565283
CAGAGTA100050.020.40873110-11
GAGTACA55950.019.9258331
GTGGTAT19650.019.8693541
AGAGTAC93750.019.8001810-11
Produced by FastQC (version 0.11.5)