FastQCFastQC Report
Wed 25 May 2016
SRR1033110_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033110_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2166490
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA28800.033.3701741
TCAACGC28550.032.413484
ATCAACG29350.031.7024753
CAACGCA30400.030.7485755
GGTATCA18750.030.653451
GTACATG65900.029.3817211
AACGCAG32600.028.5221866
TACATGG64500.028.28912
TATCAAC35050.026.700062
ACATGGG68500.025.3146463
ACGCAGA38800.023.6014187
CGCAGAG40400.022.6667088
CTAGTAC4150.020.382643
CATGGGG41100.019.3156994
CATGGGA25250.018.417874
GCAGAGT48000.018.0016299
CATGGGT14100.017.6572194
GAGTACA47700.016.4937321
AGTACAT47150.016.1577342
CGTTATA1750.002320589316.1235392
Produced by FastQC (version 0.11.5)