##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033110_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2166490
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.478783654667225	33.0	31.0	34.0	30.0	34.0
2	31.68025977502781	33.0	31.0	34.0	30.0	34.0
3	31.900009231521956	34.0	31.0	34.0	30.0	34.0
4	35.25798088151803	37.0	35.0	37.0	33.0	37.0
5	35.40174060346459	37.0	35.0	37.0	35.0	37.0
6	35.45767808759791	37.0	37.0	37.0	35.0	37.0
7	35.378898587115565	37.0	36.0	37.0	33.0	37.0
8	35.411983438649614	37.0	36.0	37.0	35.0	37.0
9	37.08349080771201	39.0	38.0	39.0	35.0	39.0
10-11	37.0760998204469	39.0	38.0	39.0	35.0	39.0
12-13	37.043430618188864	39.0	38.0	39.0	35.0	39.0
14-15	38.507936801000696	41.0	39.0	41.0	35.0	41.0
16-17	38.51877968511279	41.0	39.0	41.0	35.0	41.0
18-19	38.49903415201547	41.0	39.0	41.0	34.0	41.0
20-21	38.522744623792406	41.0	39.0	41.0	34.0	41.0
22-23	38.495722574302214	41.0	39.0	41.0	34.0	41.0
24-25	38.494139137498905	41.0	39.0	41.0	34.0	41.0
26-27	38.45446436401737	40.5	39.0	41.0	34.0	41.0
28-29	38.43529718577053	40.5	39.0	41.0	34.0	41.0
30-31	38.430840437758775	41.0	39.0	41.0	34.0	41.0
32-33	38.49970643760183	41.0	39.0	41.0	34.0	41.0
34-35	38.47770633605509	41.0	39.0	41.0	34.0	41.0
36-37	38.40138103568445	41.0	39.0	41.0	34.0	41.0
38-39	38.32322720160259	41.0	39.0	41.0	34.0	41.0
40-41	38.260231295782575	41.0	39.0	41.0	33.5	41.0
42-43	38.149475418764915	40.5	38.0	41.0	33.0	41.0
44-45	38.02342198671584	40.0	38.0	41.0	33.0	41.0
46-47	37.89779897437791	40.0	38.0	41.0	33.0	41.0
48-49	37.78160896196151	40.0	38.0	41.0	33.0	41.0
50-51	37.656719394042895	40.0	38.0	41.0	32.5	41.0
52-53	37.50792295371777	40.0	37.0	41.0	32.0	41.0
54-55	37.35097877211527	40.0	37.0	41.0	32.0	41.0
56-57	37.193624249361875	40.0	36.5	41.0	31.5	41.0
58-59	36.99547655424212	40.0	36.0	41.0	31.0	41.0
60-61	36.76558996348933	39.0	35.5	41.0	31.0	41.0
62-63	36.49488204422822	39.0	35.0	41.0	31.0	41.0
64-65	36.2182257937955	39.0	35.0	41.0	30.5	41.0
66-67	35.909794414006065	38.0	35.0	40.0	30.0	41.0
68-69	35.59032121080642	37.0	35.0	40.0	30.0	41.0
70-71	35.24402074322983	37.0	35.0	40.0	30.0	41.0
72-73	34.87280001292413	36.5	34.5	39.0	29.5	41.0
74-75	34.49488689077725	36.0	34.0	39.0	29.0	41.0
76-77	34.16133515502033	35.5	34.0	38.5	29.0	40.0
78-79	33.79692267215634	35.0	34.0	37.0	29.0	39.0
80-81	33.438505832013995	35.0	34.0	37.0	29.0	39.0
82-83	33.22636084173017	35.0	34.0	36.5	29.0	39.0
84-85	32.9619555132957	35.0	34.0	36.0	28.5	37.0
86-87	32.71739495681956	35.0	34.0	36.0	28.0	37.0
88-89	32.36857059114051	35.0	34.0	35.0	27.0	37.0
90-91	32.18720926475544	35.0	34.0	35.0	27.0	36.0
92-93	32.016507576771644	35.0	34.0	35.0	27.0	36.0
94-95	31.84950334411883	35.0	33.0	35.0	26.5	36.0
96-97	31.68076543164289	35.0	33.0	35.0	26.0	36.0
98-99	31.49050399494113	35.0	33.0	35.0	25.0	35.0
100	31.32540145581101	35.0	33.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.11969244866368456
1101	2	0.10432390425966531
1101	3	0.1782854913481806
1101	4	0.05389241366220432
1101	5	0.17507911140921806
1101	6	0.16840493901278109
1101	7	0.17506525904526882
1101	8	0.1766645996286158
1101	9	0.2577586843644184
1101	10-11	0.21441999906065234
1101	12-13	0.18225794087985747
1101	14-15	0.23016377305280855
1101	16-17	0.21889620512487085
1101	18-19	0.2858194271718588
1101	20-21	0.24761070074116276
1101	22-23	0.26645521774963754
1101	24-25	0.28078001807570274
1101	26-27	0.29524611228892894
1101	28-29	0.28903554657856745
1101	30-31	0.2950719427774118
1101	32-33	0.32563163255425565
1101	34-35	0.2529029859129821
1101	36-37	0.299793121752721
1101	38-39	0.2718741236193907
1101	40-41	0.309535282863159
1101	42-43	0.2972160575807905
1101	44-45	0.2626027703959579
1101	46-47	0.33600009163801303
1101	48-49	0.2473547524966122
1101	50-51	0.1733137646311249
1101	52-53	0.10982302844026037
1101	54-55	0.16497377254442114
1101	56-57	0.20210831165716314
1101	58-59	0.13266182421207162
1101	60-61	0.12498872089607005
1101	62-63	0.0632297722629076
1101	64-65	0.1105542857555335
1101	66-67	0.0025611436404346932
1101	68-69	0.054997555617667615
1101	70-71	0.1225147455289104
1101	72-73	0.14973869175096866
1101	74-75	0.07194082215985986
1101	76-77	0.0979652442086163
1101	78-79	0.07619743634168685
1101	80-81	0.12216459646739253
1101	82-83	0.1629309021653853
1101	84-85	0.06445599334955432
1101	86-87	0.060325594383151326
1101	88-89	0.2279994817812394
1101	90-91	0.2574485994123705
1101	92-93	0.26467735270183823
1101	94-95	0.25932973905592505
1101	96-97	0.2724366659585762
1101	98-99	0.380392052569011
1101	100	0.3930819050595602
1102	1	0.1738653780603734
1102	2	0.22561137468741066
1102	3	0.2566510216178308
1102	4	0.17002451383567063
1102	5	0.16669219344447583
1102	6	0.16876769479861764
1102	7	0.09574909461387904
1102	8	0.20118953431579456
1102	9	0.26971441431130927
1102	10-11	0.29950322518276096
1102	12-13	0.28982672810480636
1102	14-15	0.3168270009876508
1102	16-17	0.2588764979814542
1102	18-19	0.2706164140119469
1102	20-21	0.26817736975766593
1102	22-23	0.2671727042150067
1102	24-25	0.2544879864502789
1102	26-27	0.3291430598532017
1102	28-29	0.2305248111892979
1102	30-31	0.28901172867387004
1102	32-33	0.23505440816263246
1102	34-35	0.26279012669243684
1102	36-37	0.26433468781707603
1102	38-39	0.20694225414937506
1102	40-41	0.23667649662010604
1102	42-43	0.21922188517171293
1102	44-45	0.20653523403855445
1102	46-47	0.25678274616275587
1102	48-49	0.20782879605079785
1102	50-51	0.19577047731259967
1102	52-53	0.1431154704475972
1102	54-55	0.19867182335624278
1102	56-57	0.11870600437091383
1102	58-59	0.15101345898392537
1102	60-61	0.11008594112512782
1102	62-63	0.12444077234431461
1102	64-65	0.12314620207119731
1102	66-67	0.09171535408326292
1102	68-69	0.037910530735430825
1102	70-71	0.06961240551281378
1102	72-73	0.07740085554623022
1102	74-75	0.10077093789762159
1102	76-77	0.01718394713324045
1102	78-79	-0.010694533348797108
1102	80-81	0.03314167256763767
1102	82-83	0.06661137327374433
1102	84-85	0.046218440971316
1102	86-87	0.07188499947205429
1102	88-89	0.2811928236914696
1102	90-91	0.29351096193652637
1102	92-93	0.3003572376102781
1102	94-95	0.27383750526076867
1102	96-97	0.3407470527330325
1102	98-99	0.284764885790306
1102	100	0.328842030540752
1103	1	0.025789480669438092
1103	2	0.054826110432294684
1103	3	0.10745787586045097
1103	4	0.09807341460940222
1103	5	0.14335814166260263
1103	6	0.24803387876528404
1103	7	0.11741458010244088
1103	8	0.1472553588146468
1103	9	0.13126968492286295
1103	10-11	0.14370309269598636
1103	12-13	0.12562303089556082
1103	14-15	0.2621660051936203
1103	16-17	0.22727739592036045
1103	18-19	0.13690096347164626
1103	20-21	0.1539816485449279
1103	22-23	0.20354784516487
1103	24-25	0.20915662337073826
1103	26-27	0.15406917533588427
1103	28-29	0.2116294623520929
1103	30-31	0.20409613420032002
1103	32-33	0.12086888526604156
1103	34-35	0.1390629374336072
1103	36-37	0.1139242893095016
1103	38-39	0.10492058257533898
1103	40-41	0.08000037444001151
1103	42-43	0.017681848104643905
1103	44-45	-0.021597292927623357
1103	46-47	0.00909395858728601
1103	48-49	0.057019597717605564
1103	50-51	0.02799584731359772
1103	52-53	-0.022864712549775845
1103	54-55	0.027963427006447716
1103	56-57	6.851535330127945E-4
1103	58-59	0.016445764083442782
1103	60-61	-0.0671802769373997
1103	62-63	0.005542105249013218
1103	64-65	-0.0917078128384361
1103	66-67	-0.09977979821564986
1103	68-69	-0.013883977151671445
1103	70-71	0.008624648363031895
1103	72-73	0.004636478660302146
1103	74-75	-0.02314453967582608
1103	76-77	-0.08242848641026512
1103	78-79	-0.11778020163988856
1103	80-81	-0.024985837435842484
1103	82-83	0.016031987145410653
1103	84-85	-0.007141280124528748
1103	86-87	0.043239299325684044
1103	88-89	0.14752425496273247
1103	90-91	0.16835402963873491
1103	92-93	0.14313740818700893
1103	94-95	0.09429432285033457
1103	96-97	0.07810190177360354
1103	98-99	0.20847843670395605
1103	100	0.32150289603988114
1104	1	0.04423548291704549
1104	2	0.013087438627355397
1104	3	0.06256502066835523
1104	4	0.05637522450963672
1104	5	-0.06004044275608322
1104	6	-0.02597545255829914
1104	7	-0.04836749351382963
1104	8	0.09104622064109691
1104	9	0.018211739526293513
1104	10-11	0.01980578335415828
1104	12-13	0.05556140185750991
1104	14-15	-0.07815124547491337
1104	16-17	-0.003967129849051787
1104	18-19	0.015414204088315842
1104	20-21	0.015694352013916557
1104	22-23	0.032059061696280367
1104	24-25	-0.02349251103742489
1104	26-27	-0.001520466848454305
1104	28-29	0.026004719236595975
1104	30-31	0.0169059209333966
1104	32-33	0.04202262935891099
1104	34-35	-0.036894522686097275
1104	36-37	0.047425195596098035
1104	38-39	3.9018585075467627E-4
1104	40-41	0.013682625207330545
1104	42-43	0.01905469119484593
1104	44-45	-0.014171369605776363
1104	46-47	-0.026304774587146085
1104	48-49	-0.06937367991755394
1104	50-51	-0.06650840287806403
1104	52-53	-0.09765301647479419
1104	54-55	-0.07609005068193397
1104	56-57	-0.020460678226577045
1104	58-59	-0.12228397424203763
1104	60-61	-0.14806625876208557
1104	62-63	-0.12649556171864873
1104	64-65	-0.235279569856786
1104	66-67	-0.16654676847572603
1104	68-69	-0.2152431746144572
1104	70-71	-0.22608595393113973
1104	72-73	-0.24169092032622075
1104	74-75	-0.16631666091329578
1104	76-77	-0.2710203545073142
1104	78-79	-0.21761002352425862
1104	80-81	-0.16426411255106643
1104	82-83	-0.1667341471384134
1104	84-85	-0.13833415782367808
1104	86-87	-0.12912812115293804
1104	88-89	-0.01828711462438548
1104	90-91	-0.10909058112289216
1104	92-93	-0.11116198133338173
1104	94-95	-0.06760601041675685
1104	96-97	-0.10336702629021843
1104	98-99	-0.0704526177224416
1104	100	0.012047280065218047
1105	1	-0.002217384654006338
1105	2	0.04285990780215343
1105	3	0.12413374994196502
1105	4	0.0693809333872295
1105	5	0.05284252356727137
1105	6	0.06459410886187555
1105	7	-0.04166348703350309
1105	8	5.513126212051134E-4
1105	9	0.038786572581805956
1105	10-11	0.005249634817687365
1105	12-13	0.053430454735348576
1105	14-15	0.09866580142765713
1105	16-17	0.1904432974981063
1105	18-19	0.11513953202937444
1105	20-21	0.12403793262703289
1105	22-23	0.24196639219184846
1105	24-25	0.21769660803746405
1105	26-27	0.2498786989817603
1105	28-29	0.34927759361917765
1105	30-31	0.3501752790190693
1105	32-33	0.3066664979824125
1105	34-35	0.2567806670197115
1105	36-37	0.3486748485561364
1105	38-39	0.32434945937669113
1105	40-41	0.26438522891560723
1105	42-43	0.29618595678822857
1105	44-45	0.32993364384307
1105	46-47	0.35359776197991266
1105	48-49	0.30558056326182026
1105	50-51	0.2723719497332766
1105	52-53	0.24556866084941475
1105	54-55	0.20968185423679842
1105	56-57	0.35952030529497847
1105	58-59	0.3219772868065718
1105	60-61	0.3299332343066723
1105	62-63	0.3366753962540159
1105	64-65	0.2927129262119692
1105	66-67	0.3241162642469959
1105	68-69	0.2524504493294799
1105	70-71	0.16395473919711634
1105	72-73	0.13547370044386753
1105	74-75	0.20982496473614276
1105	76-77	0.18712166192504753
1105	78-79	0.2683843616408268
1105	80-81	0.280736563731935
1105	82-83	0.16571004265394151
1105	84-85	0.20513817508289378
1105	86-87	0.25342868568227317
1105	88-89	0.19781120268609698
1105	90-91	0.20529394889413766
1105	92-93	0.22653525070212055
1105	94-95	0.1503283871349872
1105	96-97	0.23015842012213383
1105	98-99	0.23148179519085588
1105	100	0.27740152684144803
1106	1	0.035058692595491436
1106	2	-0.006967315703477084
1106	3	0.017558485378529554
1106	4	0.07844718522981253
1106	5	0.03872976816428775
1106	6	0.0853095053212769
1106	7	0.015906304784770953
1106	8	0.05755378544260026
1106	9	0.06806127243439875
1106	10-11	0.05816638036731803
1106	12-13	0.08290655693556914
1106	14-15	0.05253271738273213
1106	16-17	0.008116310185407372
1106	18-19	0.05189636785394924
1106	20-21	0.006819532048936594
1106	22-23	-9.870790781576488E-4
1106	24-25	0.016396625875039206
1106	26-27	0.1002311462505503
1106	28-29	0.03565907419682901
1106	30-31	0.036237944741685624
1106	32-33	0.039704562798128507
1106	34-35	0.07348378400032374
1106	36-37	0.10239064663470998
1106	38-39	0.0762866973530123
1106	40-41	0.06872578920535233
1106	42-43	0.15732658441574898
1106	44-45	0.15720716947939195
1106	46-47	0.10840323932125528
1106	48-49	0.13871646119437742
1106	50-51	0.15638476178607874
1106	52-53	0.11474094234178267
1106	54-55	0.15267377297330142
1106	56-57	0.09095750186914131
1106	58-59	0.10687118967310028
1106	60-61	0.17008319584096654
1106	62-63	0.16986669465121906
1106	64-65	0.21799014740126665
1106	66-67	0.16948832373624612
1106	68-69	0.18999752832516492
1106	70-71	0.24181655849184125
1106	72-73	0.2351959503283254
1106	74-75	0.2683920561123472
1106	76-77	0.2230001883584123
1106	78-79	0.23933144603520873
1106	80-81	0.13475925758613982
1106	82-83	0.12759739558965322
1106	84-85	0.12062688652007125
1106	86-87	0.12137960351522281
1106	88-89	0.26718644594657803
1106	90-91	0.28317446299585924
1106	92-93	0.3112334873349667
1106	94-95	0.33527351377270165
1106	96-97	0.29279550777396324
1106	98-99	0.36460728786741825
1106	100	0.3673364980952165
1107	1	0.06225603859975948
1107	2	0.10487107234635573
1107	3	0.10188071726529913
1107	4	0.1366018243309597
1107	5	0.07657436708170451
1107	6	0.038167654569001286
1107	7	0.018233583001446618
1107	8	0.032168661333074056
1107	9	0.027852015078764225
1107	10-11	0.0415072017257998
1107	12-13	-0.015318601391236086
1107	14-15	-0.011255013278393733
1107	16-17	-0.010536420367891708
1107	18-19	0.0017425338652685696
1107	20-21	-0.04403212597954109
1107	22-23	-0.037247331682195295
1107	24-25	-0.05117549279278677
1107	26-27	0.050206166198648816
1107	28-29	0.02999273580518036
1107	30-31	0.08535915011006523
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2216	8	-0.016173406583632755
2216	9	-0.11838687004097892
2216	10-11	-0.13434913158458528
2216	12-13	-0.07230667778360811
2216	14-15	-0.1124039469381728
2216	16-17	-0.19196027217167
2216	18-19	-0.14943933884252658
2216	20-21	-0.15392695045211013
2216	22-23	-0.10303881931447734
2216	24-25	-0.1456676251127078
2216	26-27	-0.16782665158122967
2216	28-29	-0.13470332363586834
2216	30-31	-0.10467359228691464
2216	32-33	-0.1285213482559442
2216	34-35	-0.1825007937388179
2216	36-37	-0.14419419419817103
2216	38-39	-0.14537553075662402
2216	40-41	-0.1259135607875166
2216	42-43	-0.23933897836108287
2216	44-45	-0.14982081412784254
2216	46-47	-0.031948303594759864
2216	48-49	-0.03740341204227349
2216	50-51	-0.04810450518507281
2216	52-53	-0.047422773719361544
2216	54-55	-0.07112091731661252
2216	56-57	-0.12999007256293282
2216	58-59	-0.10167314760204249
2216	60-61	-0.08586688534093412
2216	62-63	-0.11080476933667427
2216	64-65	-0.1074158770774929
2216	66-67	-0.06557006743631177
2216	68-69	-0.103906815021368
2216	70-71	-0.11955270185598721
2216	72-73	2.0367755715255953E-4
2216	74-75	-0.0656944520541316
2216	76-77	-0.09222705196300751
2216	78-79	-0.11902913957834471
2216	80-81	-0.07874510967800319
2216	82-83	-0.08274708703098099
2216	84-85	-0.10291885361506559
2216	86-87	-0.10217878535756597
2216	88-89	-0.04259378798607827
2216	90-91	-0.051653146208735734
2216	92-93	-0.12126019979505287
2216	94-95	-0.09217505186045472
2216	96-97	-0.1233962562879789
2216	98-99	-0.08056905853321084
2216	100	0.015722289193266903
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	19903.0
3	3530.0
4	2171.0
5	1879.0
6	1630.0
7	1875.0
8	2251.0
9	2509.0
10	2680.0
11	2981.0
12	3329.0
13	3696.0
14	4069.0
15	4236.0
16	4340.0
17	4306.0
18	4239.0
19	4173.0
20	4219.0
21	4494.0
22	5224.0
23	6970.0
24	10852.0
25	15510.0
26	16229.0
27	13716.0
28	14271.0
29	17982.0
30	22252.0
31	26867.0
32	35821.0
33	48996.0
34	70590.0
35	108714.0
36	207581.0
37	448463.0
38	714800.0
39	298424.0
40	718.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.88791656382855	19.930559382239668	13.403669202233992	23.777854851697793
2	15.629855000402031	20.123696514704672	40.4244720208724	23.821976464020896
3	18.239282740197005	25.587805622667286	29.780165523624692	26.392746113511016
4	12.250445591644118	16.833205581670253	39.32634854963383	31.5900002770518
5	12.878786479497597	38.134096786110085	35.83394902105652	13.153167713335796
6	30.604870334150547	38.49297811613263	17.967279678382347	12.934871871334474
7	27.538196718814262	32.35172878263956	22.78056510694712	17.329509391599053
8	25.150413513095543	36.48198800200351	20.37004978776149	17.997548697139454
9	25.1367749863929	17.20043265721795	21.106396651384554	36.5563957050046
10-11	23.904566490520153	27.033100356424892	29.000050551811107	20.06228260124385
12-13	24.524290839156546	24.47614171814345	28.759428433463963	22.240139009236046
14-15	21.89135400547334	26.435159195913833	25.859295076578753	25.814191722034074
16-17	21.584832021923113	29.688345407171173	26.445190377615972	22.28163219328974
18-19	21.560792456941524	28.736397362293204	28.294502069133625	21.40830811163165
20-21	22.774585154552078	28.062400234531808	27.680801286231528	21.482213324684587
22-23	22.29590769780476	27.784117637011356	27.838822004912778	22.0811526602711
24-25	22.070614665036018	28.043512284574994	27.73744245753365	22.14843059285534
26-27	21.786081713329562	28.009370665495908	27.84679899073067	22.35774863044386
28-29	21.971563162759093	27.90379080887886	28.002699740643934	22.12194628771812
30-31	22.231816957382524	28.476419918854855	27.45662344880947	21.835139674953147
32-33	21.791339077980247	28.036582362138052	28.038706076338755	22.133372483542946
34-35	21.989929886825482	27.753770175772267	28.09531107758613	22.160988859816122
36-37	22.384726083439215	28.100627450165778	27.446888311100803	22.067758155294204
38-39	22.092525126440048	28.30463696610354	27.73810337155002	21.86473453590639
40-41	21.889019345990622	27.938787281348116	27.994250142350058	22.177943230311204
42-43	21.828697698508172	27.93881275925134	27.952568717473852	22.279920824766634
44-45	21.89527051355199	28.034287576558263	27.92904016204409	22.14140174784566
46-47	22.011836122260895	28.146253498709456	27.75510891785179	22.086801461177856
48-49	21.939786069943395	28.042973418223426	27.85558959550113	22.16165091633205
50-51	21.968640527926297	27.91789741608401	27.758113483289737	22.35534857269996
52-53	21.99139842324429	27.901370371413257	27.848308696621547	22.2589225087209
54-55	22.04022323315106	28.38114884156433	27.63312253382228	21.94550539146233
56-57	21.940918824081688	28.044218103130337	27.651454480916414	22.36340859187156
58-59	21.889079831853255	27.51058071740703	28.02488043754711	22.575459013192607
60-61	22.036258276579044	28.078619682754503	27.765916222983485	22.119205817682968
62-63	21.866092822283182	28.076785843694797	27.734034760235797	22.323086573786224
64-65	21.897895291957706	27.85793826543624	27.762898101850837	22.48126834075522
66-67	21.808159172289322	27.828701924685806	27.750463602447773	22.612675300577095
68-69	22.175978656105503	28.011091993616215	27.69524943714941	22.11767991312887
70-71	22.161595660736307	27.7985349769127	27.759531085524458	22.28033827682654
72-73	21.939012895703495	27.864237386554457	27.806862630154843	22.389887087587205
74-75	21.934337355654	27.916628682142814	27.694399023288067	22.454634938915117
76-77	21.96510499957649	27.91373853606433	27.65083858644714	22.47031787791204
78-79	22.11175846626373	28.058268426003796	27.61131026180495	22.218662845927525
80-81	22.07033071119108	27.78809945615397	27.754195627629336	22.387374205025615
82-83	21.91450883151484	27.873346485442056	27.889155787866393	22.32298889517671
84-85	21.886716421320692	27.79989941980599	27.7671727756787	22.546211383194613
86-87	22.14461430024434	27.809400236470296	27.674894198815526	22.371091264469843
88-89	21.93635524949171	27.8437310073967	27.690536582104457	22.529377161007133
90-91	21.873948672584863	27.697115546469686	27.861357702652974	22.567578078292478
92-93	22.040791024646563	27.89569864038431	27.671305517563376	22.392204817405755
94-95	21.84808131362783	27.58170420159874	27.89098291109195	22.67923157368148
96-97	21.9468542143562	27.631922570419178	27.805282079459847	22.61594113576478
98-99	21.807399628500452	27.74909434797929	27.72102992934452	22.72247609417574
100	21.933612420476646	27.57971834700062	27.689332089713346	22.79733714280939
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	225.0
1	225.0
2	215.5
3	190.5
4	160.5
5	135.0
6	117.5
7	105.5
8	109.5
9	113.0
10	111.0
11	116.0
12	130.5
13	150.0
14	169.5
15	194.5
16	270.5
17	386.0
18	569.0
19	830.5
20	1175.5
21	1750.0
22	2681.0
23	4112.5
24	5996.5
25	8233.0
26	11405.5
27	15571.0
28	20495.5
29	25818.5
30	31041.5
31	36870.0
32	42918.0
33	49010.0
34	54592.5
35	60608.0
36	66304.0
37	71483.5
38	75877.0
39	77714.0
40	80148.0
41	81917.5
42	82396.5
43	83706.0
44	82783.5
45	80969.5
46	80587.5
47	78879.0
48	76989.0
49	74976.0
50	72607.0
51	69451.0
52	62715.0
53	57038.0
54	54308.0
55	51429.5
56	47008.0
57	42118.5
58	38250.5
59	33984.5
60	29291.5
61	24914.0
62	21518.0
63	18280.0
64	15036.0
65	12304.5
66	9947.5
67	7861.0
68	6106.5
69	4834.5
70	3643.0
71	2780.0
72	2264.0
73	1844.0
74	1522.5
75	1146.5
76	783.5
77	601.0
78	472.0
79	316.0
80	237.0
81	152.0
82	85.0
83	57.5
84	36.5
85	22.5
86	18.5
87	14.5
88	13.0
89	11.0
90	8.0
91	5.5
92	4.0
93	4.0
94	2.5
95	2.0
96	2.0
97	2.0
98	4.0
99	3.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.32065691510230837
2	0.11465550267944924
3	0.06282050690287054
4	0.03831081611269842
5	0.041080272699158545
6	0.00881610346689807
7	0.006415907758632627
8	0.013616494883428957
9	0.01573974493304839
10-11	0.01825533466574967
12-13	0.01398575576162364
14-15	0.01633979386011475
16-17	0.017262946055601455
18-19	0.016893685177406775
20-21	0.021809470618373496
22-23	0.013893440542074968
24-25	0.02220181030145535
26-27	0.017009079201842612
28-29	0.01643210907966342
30-31	0.01714755203116562
32-33	0.021855628228147836
34-35	0.012831815517265255
36-37	0.008423763783816218
38-39	0.01135477200448652
40-41	0.008100660515395871
42-43	0.00720058712479633
44-45	0.007662163222539684
46-47	0.007177508319909162
48-49	0.0037387663917211713
50-51	0.0058620164413406015
52-53	0.006600538197729969
54-55	0.0025617473424756174
56-57	0.002169407659393766
58-59	0.002261722878942437
60-61	0.0027925353913472945
62-63	0.002261722878942437
64-65	0.0017309103665375794
66-67	0.001500122317665902
68-69	0.0028848506108959653
70-71	0.00182322558608625
72-73	0.0014770435127787343
74-75	0.0017309103665375794
76-77	0.0035310571477366617
78-79	0.00313871746465481
80-81	0.00313871746465481
82-83	0.0020309348300707594
84-85	0.002746377781572959
86-87	0.0020540136349579273
88-89	0.5857862256460912
90-91	0.5983180166998232
92-93	0.5955947177231374
94-95	0.5819320652299341
96-97	0.5808704402051245
98-99	0.5777548015453567
100	0.5798780515949762
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2166490.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.89030051782275
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.67218724048793	55.44749335704615
2	11.749420653157753	15.953433981063863
3	3.2025998789427668	6.522764046593017
4	1.2333285500796385	3.349241836084691
5	0.5907018428767948	2.005146281466866
6	0.35104171703054987	1.429939659809792
7	0.2309736646963276	1.0976610055555558
8	0.14471897089568883	0.7860011539790688
9	0.11351362597781688	0.6935826762451548
>10	0.6409886291688274	8.036460130933227
>50	0.04840110055267847	2.265338219101361
>100	0.02205614363040901	2.387137831381877
>500	6.798250283319839E-5	0.025799820739404632
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	1.3847282932300634E-4	0.0
10-11	0.0	0.0	0.0	1.6155163421017408E-4	0.0
12-13	0.0	0.0	0.0	1.846304390973418E-4	0.0
14-15	0.0	0.0	0.0	1.846304390973418E-4	0.0
16-17	0.0	0.0	0.0	1.846304390973418E-4	0.0
18-19	0.0	0.0	0.0	2.3078804887167724E-4	0.0
20-21	0.0	2.3078804887167724E-5	0.0	2.769456586460127E-4	0.0
22-23	0.0	4.615760977433545E-5	0.0	3.692608781946836E-4	0.0
24-25	0.0	4.615760977433545E-5	0.0	4.384972928561867E-4	0.0
26-27	0.0	4.615760977433545E-5	0.0	6.69285341727864E-4	0.0
28-29	0.0	4.615760977433545E-5	0.0	0.001661673951876076	0.0
30-31	0.0	4.615760977433545E-5	0.0	0.005538913172920254	0.0
32-33	0.0	4.615760977433545E-5	0.0	0.014793513932674512	0.0
34-35	0.0	4.615760977433545E-5	0.0	0.02797151152324728	0.0
36-37	0.0	6.923641466150317E-5	0.0	0.051581128922819866	0.0
38-39	0.0	9.23152195486709E-5	0.0	0.09132283093852268	0.0
40-41	0.0	9.23152195486709E-5	0.0	0.1377112287617298	0.0
42-43	0.0	9.23152195486709E-5	0.0	0.1864998222932024	0.0
44-45	0.0	9.23152195486709E-5	0.0	0.2400426496314315	0.0
46-47	0.0	9.23152195486709E-5	0.0	0.306163425633167	0.0
48-49	0.0	9.23152195486709E-5	0.0	0.3770153566367719	0.0
50-51	0.0	9.23152195486709E-5	0.0	0.45901435040087885	0.0
52-53	0.0	9.23152195486709E-5	0.0	0.542836569751072	0.0
54-55	0.0	9.23152195486709E-5	0.0	0.6273511532478802	0.0
56-57	0.0	9.23152195486709E-5	0.0	0.7295902588980332	0.0
58-59	0.0	9.23152195486709E-5	0.0	0.8509616937996483	0.0
60-61	0.0	9.23152195486709E-5	0.0	0.9774104657764402	0.0
62-63	0.0	9.23152195486709E-5	0.0	1.106997955217887	0.0
64-65	0.0	9.23152195486709E-5	0.0	1.242124357832254	0.0
66-67	0.0	9.23152195486709E-5	0.0	1.3984832609428155	0.0
68-69	0.0	9.23152195486709E-5	0.0	1.5760285069397966	0.0
70-71	0.0	9.23152195486709E-5	0.0	1.76749027228374	0.0
72-73	0.0	9.23152195486709E-5	0.0	1.9660833883378184	0.0
74-75	0.0	1.3847282932300634E-4	0.0	2.1704231268088012	0.0
76-77	0.0	1.3847282932300634E-4	0.0	2.386971553065096	0.0
78-79	0.0	1.3847282932300634E-4	0.0	2.6340301593822266	0.0
80-81	0.0	1.3847282932300634E-4	0.0	2.901675059658711	0.0
82-83	0.0	1.3847282932300634E-4	0.0	3.189352362577256	0.0
84-85	0.0	1.3847282932300634E-4	0.0	3.466828833735674	0.0
86-87	0.0	1.3847282932300634E-4	0.0	3.7657916722440445	0.0
88	0.0	1.3847282932300634E-4	0.0	4.00620358275367	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	2880	0.0	33.370174	1
TCAACGC	2855	0.0	32.41348	4
ATCAACG	2935	0.0	31.702475	3
CAACGCA	3040	0.0	30.748575	5
GGTATCA	1875	0.0	30.65345	1
GTACATG	6590	0.0	29.381721	1
AACGCAG	3260	0.0	28.522186	6
TACATGG	6450	0.0	28.2891	2
TATCAAC	3505	0.0	26.70006	2
ACATGGG	6850	0.0	25.314646	3
ACGCAGA	3880	0.0	23.601418	7
CGCAGAG	4040	0.0	22.666708	8
CTAGTAC	415	0.0	20.38264	3
CATGGGG	4110	0.0	19.315699	4
CATGGGA	2525	0.0	18.41787	4
GCAGAGT	4800	0.0	18.001629	9
CATGGGT	1410	0.0	17.657219	4
GAGTACA	4770	0.0	16.493732	1
AGTACAT	4715	0.0	16.157734	2
CGTTATA	175	0.0023205893	16.123539	2
>>END_MODULE