FastQCFastQC Report
Wed 25 May 2016
SRR1033110_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033110_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2166490
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38710.1786761074364525No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32650.15070459591320523No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29750.050.2933581
GTATCAA58000.043.968511
TCAACGC67500.037.4007074
ATCAACG67650.037.2482873
CAACGCA69150.036.5091255
AACGCAG72400.034.9871986
TATCAAC73450.034.179742
ACGCAGA82400.030.5130427
CGCAGAG83850.029.9846958
GCAGAGT92500.026.8250859
GTACATG65500.024.0643391
TACATGG64950.022.8732172
ACATGGG65300.021.958163
GTGGTAT14100.021.6902561
AGAGTAC85900.021.3363610-11
GAGTACT60300.021.23675212-13
CAGAGTA92250.020.73370710-11
AGTACTT63300.019.41363512-13
GGGTACG1708.560058E-519.3740121
GTACTTT65300.019.25083214-15
Produced by FastQC (version 0.11.5)