FastQCFastQC Report
Wed 25 May 2016
SRR1033109_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033109_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1982291
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15550.032.4208831
TCAACGC22900.029.543234
GTATCAA24250.029.5327111
CAACGCA24050.028.1298475
ATCAACG24400.027.9224153
TACGTCG856.834185E-427.6357385
GTACATG56700.027.5052991
TACATGG55850.026.5103632
AACGCAG25950.026.2387056
ACATGGG57600.024.2275093
TATCAAC30250.022.6858882
ACGCAGA31900.020.9030387
CATGGGG37950.019.9317324
CGCAGAG33400.019.8236858
TATATCG1508.316178E-418.7923015
GAGTACA39550.018.107921
GTATAGA8800.017.6686231
CCTATAC9150.017.4595323
AGAGTAC33750.016.48755810-11
GCAGAGT40200.016.4704279
Produced by FastQC (version 0.11.5)