##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1033109_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1982291 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.4171481381896 33.0 31.0 34.0 30.0 34.0 2 31.492706671220322 33.0 31.0 34.0 29.0 34.0 3 31.685676320984154 34.0 31.0 34.0 30.0 34.0 4 35.03419275979157 37.0 35.0 37.0 33.0 37.0 5 35.14991290380676 37.0 35.0 37.0 33.0 37.0 6 35.18652609531093 37.0 35.0 37.0 33.0 37.0 7 35.13245078548003 37.0 35.0 37.0 33.0 37.0 8 35.15392694614464 37.0 35.0 37.0 33.0 37.0 9 36.78876461629498 39.0 38.0 39.0 33.0 39.0 10-11 36.77887933709027 39.0 38.0 39.0 33.0 39.0 12-13 36.719483668139546 39.0 38.0 39.0 33.0 39.0 14-15 38.15025039209682 41.0 39.0 41.0 33.5 41.0 16-17 38.153651507271135 41.0 39.0 41.0 33.0 41.0 18-19 38.13430772777559 41.0 39.0 41.0 33.5 41.0 20-21 38.17154317907915 41.0 39.0 41.0 34.0 41.0 22-23 38.12671625911635 40.0 39.0 41.0 33.0 41.0 24-25 38.12657954861319 40.5 39.0 41.0 33.0 41.0 26-27 38.091667923629785 40.0 38.0 41.0 33.0 41.0 28-29 38.06005298919281 40.0 38.0 41.0 33.0 41.0 30-31 38.05761464890877 40.0 38.0 41.0 33.0 41.0 32-33 38.125822596177855 41.0 39.0 41.0 33.0 41.0 34-35 38.09048848024836 41.0 38.5 41.0 33.0 41.0 36-37 38.00430638084923 41.0 38.0 41.0 33.0 41.0 38-39 37.920411029460354 40.5 38.0 41.0 33.0 41.0 40-41 37.854305447585645 40.0 38.0 41.0 33.0 41.0 42-43 37.72978614138893 40.0 38.0 41.0 32.5 41.0 44-45 37.5836317674852 40.0 38.0 41.0 32.0 41.0 46-47 37.451123725023216 40.0 38.0 41.0 32.0 41.0 48-49 37.331401393639986 40.0 37.5 41.0 31.0 41.0 50-51 37.20682760502873 40.0 37.0 41.0 31.0 41.0 52-53 37.05585960890707 40.0 37.0 41.0 31.0 41.0 54-55 36.90299279974535 40.0 36.5 41.0 30.5 41.0 56-57 36.75005511299804 40.0 36.0 41.0 30.0 41.0 58-59 36.55582328729737 40.0 36.0 41.0 30.0 41.0 60-61 36.33735410189523 39.0 35.0 41.0 30.0 41.0 62-63 36.07903683162563 39.0 35.0 41.0 30.0 41.0 64-65 35.817055618978245 39.0 35.0 41.0 29.5 41.0 66-67 35.536981956735914 38.0 35.0 40.0 29.0 41.0 68-69 35.23314740368593 37.5 35.0 40.0 29.0 41.0 70-71 34.893927783559526 37.0 34.5 40.0 29.0 41.0 72-73 34.53172793500046 36.5 34.0 39.0 28.5 41.0 74-75 34.163539056576454 36.0 34.0 39.0 28.0 41.0 76-77 33.83448797376369 35.5 34.0 38.5 28.0 40.0 78-79 33.46983666878374 35.0 34.0 37.0 27.5 39.5 80-81 33.101994863519025 35.0 34.0 37.0 27.0 39.0 82-83 32.878194725194234 35.0 34.0 36.5 27.0 39.0 84-85 32.60273970875114 35.0 34.0 36.0 27.0 37.5 86-87 32.33909299895929 35.0 33.0 36.0 26.0 37.0 88-89 31.970910174136897 35.0 33.0 35.5 26.0 37.0 90-91 31.774380502156344 35.0 33.0 35.0 25.0 36.0 92-93 31.592454639606395 35.0 33.0 35.0 25.0 36.0 94-95 31.412838982772964 35.0 33.0 35.0 25.0 36.0 96-97 31.224478898405934 35.0 33.0 35.0 24.0 36.0 98-99 31.022930790686132 35.0 33.0 35.0 23.0 35.0 100 30.855003629638635 35.0 33.0 35.0 20.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.08560565021930344 1101 2 0.09560734080780975 1101 3 0.0036189968138451434 1101 4 -4.004375872099786E-4 1101 5 -0.002024579454371178 1101 6 0.06767037102863327 1101 7 0.04782422263079411 1101 8 0.004694848953924691 1101 9 0.15135408481111767 1101 10-11 0.0826538102500507 1101 12-13 0.06503056980339039 1101 14-15 0.15405564067206257 1101 16-17 0.0713975748769684 1101 18-19 0.02848590987174049 1101 20-21 0.022216836800481587 1101 22-23 0.052366712911236846 1101 24-25 -0.04588330466710033 1101 26-27 -0.044960613889927004 1101 28-29 -0.04996093120641376 1101 30-31 0.04471182916871186 1101 32-33 0.026798833854385862 1101 34-35 -0.04297962800676913 1101 36-37 -0.06273619649955009 1101 38-39 -0.06932671431081872 1101 40-41 -0.07224396641096575 1101 42-43 -3.3238306878047297E-4 1101 44-45 -0.11473019153081054 1101 46-47 -0.13866365276519588 1101 48-49 -0.09874683548617469 1101 50-51 -0.12487116494662587 1101 52-53 -0.09326940545313533 1101 54-55 -0.17126191765600396 1101 56-57 -0.13560552876194976 1101 58-59 -0.18222858437945177 1101 60-61 -0.18399045071073772 1101 62-63 -0.12284435418521866 1101 64-65 -0.12031296484186527 1101 66-67 -0.12474344029093487 1101 68-69 -0.10466811342642757 1101 70-71 -0.10743079809375189 1101 72-73 -0.014841894321904192 1101 74-75 -0.10211018597554045 1101 76-77 -0.14959855664913135 1101 78-79 -0.2083671731596084 1101 80-81 -0.18900759844996173 1101 82-83 -0.29579350705837726 1101 84-85 -0.30154068133150247 1101 86-87 -0.15733028976161023 1101 88-89 -0.06658692991238624 1101 90-91 0.033623633154476806 1101 92-93 0.03828809047833559 1101 94-95 0.08377162710788255 1101 96-97 0.03652182676300697 1101 98-99 0.09135173747139547 1101 100 0.22576016472338267 1102 1 -0.07694787104389533 1102 2 -0.04054300308446912 1102 3 -0.014833003573642145 1102 4 0.017679577912154798 1102 5 -0.15835510352683002 1102 6 -0.19895173494831653 1102 7 -0.13741843938606735 1102 8 -0.16389955189854533 1102 9 -0.06308745350138878 1102 10-11 -0.09727353645148895 1102 12-13 -0.11379050934027646 1102 14-15 -0.009058764055247082 1102 16-17 -0.0676521199791722 1102 18-19 -0.03889857138371866 1102 20-21 -0.11757973396382937 1102 22-23 -0.10936146688145243 1102 24-25 -0.11464141761875624 1102 26-27 -0.06695518911030263 1102 28-29 -0.007239823001427226 1102 30-31 0.004483213456225599 1102 32-33 -0.00651706268732255 1102 34-35 -0.06316562045082463 1102 36-37 -0.10742766215876287 1102 38-39 -0.08038938988961775 1102 40-41 -0.14601321953504964 1102 42-43 -0.10429731574338774 1102 44-45 -0.201733000791684 1102 46-47 -0.11339386685024522 1102 48-49 -0.12902388673096965 1102 50-51 -0.0884050213837071 1102 52-53 -0.20290710767147857 1102 54-55 -0.11898932248018212 1102 56-57 -0.12835182344372953 1102 58-59 -0.20289311988270242 1102 60-61 -0.16479448054703028 1102 62-63 -0.027859369200236017 1102 64-65 -0.18739520837572599 1102 66-67 -0.2552713875930692 1102 68-69 -0.261141037963867 1102 70-71 -0.1472060572238547 1102 72-73 -0.09687037441490531 1102 74-75 -0.1326090719582993 1102 76-77 -0.15243784755293888 1102 78-79 -0.1293455709122 1102 80-81 -0.23461158953782046 1102 82-83 -0.26454290161422733 1102 84-85 -0.22653825955489282 1102 86-87 -0.21500150936832085 1102 88-89 -0.15826086190889654 1102 90-91 -0.1659840390338445 1102 92-93 -0.10020265833499309 1102 94-95 -0.0824562136360889 1102 96-97 -0.12355179524610094 1102 98-99 -0.15754683852395246 1102 100 -0.10986320057740073 1103 1 0.15592135053500655 1103 2 0.17947813467860385 1103 3 0.23864094183579 1103 4 0.25130010911333045 1103 5 0.1832472308174431 1103 6 0.20530338866164755 1103 7 0.15992555473212633 1103 8 0.14813059238966275 1103 9 0.21102567948271655 1103 10-11 0.2460818536780991 1103 12-13 0.26136113113394543 1103 14-15 0.2649544240708437 1103 16-17 0.2426628786422782 1103 18-19 0.31267182655765424 1103 20-21 0.32931393389758057 1103 22-23 0.291331254375784 1103 24-25 0.17333418205039663 1103 26-27 0.2837777398484249 1103 28-29 0.3240854228399428 1103 30-31 0.2048531243100129 1103 32-33 0.19866743322297964 1103 34-35 0.1782448657177298 1103 36-37 0.17116116239780155 1103 38-39 0.13836463088053108 1103 40-41 0.17455244538545145 1103 42-43 0.10709754686114792 1103 44-45 0.038252983952368425 1103 46-47 0.11240684080529917 1103 48-49 0.14408060734127304 1103 50-51 0.09476173718627479 1103 52-53 -0.00856720325093363 1103 54-55 0.11272995883587811 1103 56-57 -0.051872180028603054 1103 58-59 -0.08605298570385855 1103 60-61 -0.1324858692061568 1103 62-63 -0.05104312988399329 1103 64-65 -0.015772655301553584 1103 66-67 0.007528446980956005 1103 68-69 -0.0026491714074836636 1103 70-71 -0.030807058970012235 1103 72-73 0.03402177704176523 1103 74-75 -0.036548277913631466 1103 76-77 -0.0717593713099447 1103 78-79 -0.04930926910170541 1103 80-81 -0.014583559025922455 1103 82-83 0.014789191024320303 1103 84-85 0.11051945572863531 1103 86-87 0.0839852565539303 1103 88-89 0.12009590677044812 1103 90-91 0.18558521011605222 1103 92-93 0.08769826988851648 1103 94-95 0.0707351290713838 1103 96-97 -0.04130292106174238 1103 98-99 0.04301147413113071 1103 100 0.047321341284558116 1104 1 -0.005777299662433677 1104 2 0.037664361508898736 1104 3 0.01882007128852692 1104 4 -0.11292398846427432 1104 5 -0.10327699063414286 1104 6 -0.08244097172193676 1104 7 -0.12404099436759708 1104 8 -0.054590101856454964 1104 9 -0.10790705803356104 1104 10-11 -0.09393298754739732 1104 12-13 -0.1064015541226837 1104 14-15 -0.1506796515208677 1104 16-17 -0.1100861849118715 1104 18-19 -0.10117657170358285 1104 20-21 -0.08931924442082817 1104 22-23 -0.10503460400580877 1104 24-25 -0.14630704906178238 1104 26-27 -0.10521252958977811 1104 28-29 -0.09212028876528677 1104 30-31 -0.07304648258475765 1104 32-33 -0.16481552696094326 1104 34-35 -0.12040369337271528 1104 36-37 -0.1108959093081765 1104 38-39 -0.19211754388130942 1104 40-41 -0.24169354743439442 1104 42-43 -0.2784532365407202 1104 44-45 -0.3034097531401514 1104 46-47 -0.37427270353162356 1104 48-49 -0.397902026651046 1104 50-51 -0.40617169065393455 1104 52-53 -0.36143834229035576 1104 54-55 -0.3998332839029146 1104 56-57 -0.3125779906738799 1104 58-59 -0.40437479146513056 1104 60-61 -0.33736105410555695 1104 62-63 -0.4110043786164326 1104 64-65 -0.3823099047080589 1104 66-67 -0.3553687110130568 1104 68-69 -0.41180283721974575 1104 70-71 -0.42724781331028794 1104 72-73 -0.30808984306622733 1104 74-75 -0.3601330029814065 1104 76-77 -0.4488354552564857 1104 78-79 -0.42409295774738354 1104 80-81 -0.33626404202601634 1104 82-83 -0.3174596907100309 1104 84-85 -0.31405575607418257 1104 86-87 -0.25727756321978035 1104 88-89 -0.12400308006461458 1104 90-91 -0.13082988989469158 1104 92-93 -0.3057023614055794 1104 94-95 -0.25715950118402375 1104 96-97 -0.19791209522539432 1104 98-99 -0.14624851930561888 1104 100 -0.22693546414657817 1105 1 0.14283766216984262 1105 2 0.04370069516523856 1105 3 0.083795304134469 1105 4 0.13034324365800387 1105 5 0.06058664999411434 1105 6 -0.02796491625456099 1105 7 9.456552199793578E-4 1105 8 -0.025238666957072553 1105 9 0.16273734114831484 1105 10-11 0.11773675399215477 1105 12-13 0.19062851165517003 1105 14-15 0.1304365456302392 1105 16-17 0.12688514023147945 1105 18-19 0.09807713876695345 1105 20-21 0.04574556511938255 1105 22-23 0.18122892210098485 1105 24-25 0.16961126871898102 1105 26-27 0.17169083797135443 1105 28-29 0.1495599407402537 1105 30-31 0.18337661473216826 1105 32-33 0.1312020907448499 1105 34-35 0.11395767777393928 1105 36-37 0.1161899148728196 1105 38-39 0.11122784229287674 1105 40-41 0.04157771606988092 1105 42-43 0.06771726330226358 1105 44-45 0.09653780112919463 1105 46-47 0.11893720295234544 1105 48-49 0.11137977013684974 1105 50-51 0.078097658533963 1105 52-53 0.06038205562923338 1105 54-55 0.026602845776828588 1105 56-57 0.048691132279849114 1105 58-59 0.054788760498688305 1105 60-61 0.08847296459812526 1105 62-63 0.08588451798274832 1105 64-65 0.028660635165508097 1105 66-67 0.04270831033379352 1105 68-69 0.07720893250384364 1105 70-71 0.008393521620021716 1105 72-73 0.06491300899492103 1105 74-75 -0.006871590163761709 1105 76-77 0.1087143519606073 1105 78-79 0.05420219252132341 1105 80-81 -0.018364611553131738 1105 82-83 0.014635550453583335 1105 84-85 0.04635091548537673 1105 86-87 0.08654957782347594 1105 88-89 0.2578037862900011 1105 90-91 0.1968729623198726 1105 92-93 0.08883480915197595 1105 94-95 0.05771668782878692 1105 96-97 0.05539039850772909 1105 98-99 0.04510776576170983 1105 100 0.155324283601729 1106 1 0.0024190418602820785 1106 2 -0.009943714226004374 1106 3 -0.08106138648722805 1106 4 -0.0859260427679871 1106 5 -0.039736362855805396 1106 6 -0.06371861742932339 1106 7 -0.058416129126804606 1106 8 -0.10114487671339134 1106 9 0.004927339763682426 1106 10-11 -0.0611867876422707 1106 12-13 -0.12375160096154048 1106 14-15 -0.07513301084417634 1106 16-17 -0.16448050479421283 1106 18-19 -0.16985961071516442 1106 20-21 -0.12424240758979721 1106 22-23 -0.16057340227025207 1106 24-25 -0.09460716778750822 1106 26-27 -0.017737634942811553 1106 28-29 0.010731950003716406 1106 30-31 -0.022072408650011255 1106 32-33 -0.015603369409888046 1106 34-35 0.031404557239490316 1106 36-37 -3.9301777706413077E-4 1106 38-39 0.001894938376352684 1106 40-41 -0.034726462169317074 1106 42-43 -0.025110921554215793 1106 44-45 8.905220209385334E-4 1106 46-47 -0.007294259499246891 1106 48-49 -0.0012001176808311698 1106 50-51 0.04166221856951324 1106 52-53 0.019566699882972216 1106 54-55 -0.0025932556252783456 1106 56-57 -0.025132077857470847 1106 58-59 0.017260208126579357 1106 60-61 0.08664788656552957 1106 62-63 0.0885825700865297 1106 64-65 0.05698743901026404 1106 66-67 6.945241987565964E-4 1106 68-69 -0.002741272792690097 1106 70-71 0.008329889649687061 1106 72-73 -0.019906654041832894 1106 74-75 0.06664211406985743 1106 76-77 -0.008497999858917638 1106 78-79 -0.03537125947404007 1106 80-81 -0.03803871179141538 1106 82-83 -0.028406358636750895 1106 84-85 0.06874840749206612 1106 86-87 0.04172914102196046 1106 88-89 0.17388311573007442 1106 90-91 0.20377830374017947 1106 92-93 0.1964822860001192 1106 94-95 0.1758043893130541 1106 96-97 0.14130956543005624 1106 98-99 0.11938194274297942 1106 100 0.17492474043968187 1107 1 0.07285108819165842 1107 2 0.04557919871679772 1107 3 -0.04220553105390579 1107 4 -0.06469502687198059 1107 5 -0.09214121868691905 1107 6 1.8917667120632586E-4 1107 7 -0.04437705526792968 1107 8 -0.026489773782763848 1107 9 -0.10693969029994577 1107 10-11 -0.08491322633467036 1107 12-13 -0.10803461719107332 1107 14-15 -0.14354059996936996 1107 16-17 -0.12003820274740917 1107 18-19 -0.013115357407329498 1107 20-21 -0.08777112758826178 1107 22-23 -0.057856653874011954 1107 24-25 -0.0587626476643166 1107 26-27 -0.025015128129119546 1107 28-29 -0.10635608864282631 1107 30-31 -0.016297923216285426 1107 32-33 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-0.08047674687161077 2214 3 -0.03244389636967071 2214 4 0.11213823697480763 2214 5 -0.0013019172531087975 2214 6 0.10411390225815609 2214 7 0.1143692231835729 2214 8 0.14844098958722185 2214 9 0.17012669159928606 2214 10-11 0.18728375596948865 2214 12-13 0.16277537836531764 2214 14-15 0.16957033525290655 2214 16-17 0.1318199008245884 2214 18-19 0.22874472001381463 2214 20-21 0.17967754204328656 2214 22-23 0.1554209314518431 2214 24-25 0.16337167914041828 2214 26-27 0.14730690055657902 2214 28-29 0.18795408048292472 2214 30-31 0.16845489579115736 2214 32-33 0.16373458433099586 2214 34-35 0.14711299679402856 2214 36-37 0.1768910392366294 2214 38-39 0.12195307191061033 2214 40-41 0.2336928114324337 2214 42-43 0.28134608078867274 2214 44-45 0.26760810606040053 2214 46-47 0.3174678393974304 2214 48-49 0.3072553055354206 2214 50-51 0.3547685825530351 2214 52-53 0.24017804379197827 2214 54-55 0.28426528868832435 2214 56-57 0.31351382105778924 2214 58-59 0.33607542450820915 2214 60-61 0.38359658559225096 2214 62-63 0.4231516388146659 2214 64-65 0.22517732640718435 2214 66-67 0.2326564858249469 2214 68-69 0.22900136569052165 2214 70-71 0.29969874989182443 2214 72-73 0.31183766161834825 2214 74-75 0.35263368690062435 2214 76-77 0.28582720733695055 2214 78-79 0.26213413329499957 2214 80-81 0.29789080549124947 2214 82-83 0.25510105706575814 2214 84-85 0.19023472172794698 2214 86-87 0.15873416739233903 2214 88-89 0.19333578837609267 2214 90-91 0.04227454686375509 2214 92-93 0.10020007130082575 2214 94-95 0.15548603532034733 2214 96-97 0.2882696176547661 2214 98-99 0.1706055078535691 2214 100 0.21515892595093788 2215 1 -0.12332591292278394 2215 2 -0.08040340678188684 2215 3 -0.0696811454336661 2215 4 -0.00798539096164319 2215 5 0.06026402493949945 2215 6 0.04935047218241806 2215 7 0.019589672290976523 2215 8 0.01806611495676691 2215 9 -0.014304205057698027 2215 10-11 -0.08017770547620273 2215 12-13 -0.11440360120973025 2215 14-15 -0.1244049251569308 2215 16-17 -0.07956496661188339 2215 18-19 -0.05093403639031635 2215 20-21 0.0030956103108294997 2215 22-23 0.0023580200078185953 2215 24-25 0.024903007961327717 2215 26-27 -0.04954809610898536 2215 28-29 0.04850437620626735 2215 30-31 -0.0024305763947509718 2215 32-33 -0.016741548633362413 2215 34-35 0.010174315410331758 2215 36-37 -0.18265875063263337 2215 38-39 -0.09408963512636603 2215 40-41 -0.08294493053297458 2215 42-43 -0.10988588393806964 2215 44-45 -0.04198955615859745 2215 46-47 0.05230600109919692 2215 48-49 0.15510174994135895 2215 50-51 0.04525843834086629 2215 52-53 -0.03763640403066404 2215 54-55 -0.022097226649201218 2215 56-57 0.016653835865831468 2215 58-59 0.0820625488590423 2215 60-61 -0.09804292423689276 2215 62-63 0.003280981676958561 2215 64-65 0.015401433767273431 2215 66-67 0.010654852738937848 2215 68-69 0.09326850740492887 2215 70-71 0.10211954171974469 2215 72-73 0.1426268902521457 2215 74-75 0.11939526684569302 2215 76-77 -0.004168653061327632 2215 78-79 0.025615757139114237 2215 80-81 -0.06827976535370084 2215 82-83 -0.07192358450424052 2215 84-85 -0.09582707298631732 2215 86-87 -0.1028905869008554 2215 88-89 0.027968151484802917 2215 90-91 -0.01226304299535741 2215 92-93 -0.08502627652023875 2215 94-95 -0.14133189746932473 2215 96-97 -0.10705490786635963 2215 98-99 -0.11854947953508699 2215 100 -0.04346063502373099 2216 1 0.14502298963664373 2216 2 0.10901711421758264 2216 3 0.058704211899058834 2216 4 0.11774940056262295 2216 5 -0.016587194016985052 2216 6 0.17004235340061058 2216 7 0.11909601390258473 2216 8 0.1638852081442792 2216 9 0.09581958927662271 2216 10-11 0.08369979129603422 2216 12-13 0.06304000781282326 2216 14-15 0.024877371493793987 2216 16-17 0.0766969001763016 2216 18-19 0.03104743243326169 2216 20-21 0.03318598776963455 2216 22-23 0.0257593263038558 2216 24-25 0.041875363091577356 2216 26-27 -0.09107426000356611 2216 28-29 0.027766926521451296 2216 30-31 -0.039830895374009856 2216 32-33 -0.03936629231074562 2216 34-35 -0.04519327022040187 2216 36-37 -0.042736976500336255 2216 38-39 -0.041125786109887486 2216 40-41 -0.031853186020178725 2216 42-43 -0.011691534427932027 2216 44-45 0.05180933500871987 2216 46-47 0.1385728044712664 2216 48-49 0.07419493745560146 2216 50-51 0.012809892734424011 2216 52-53 0.07227094008720769 2216 54-55 0.06486069846661024 2216 56-57 -0.0025779557343739157 2216 58-59 -0.023379085529960264 2216 60-61 -0.021620808341097586 2216 62-63 0.0774075860667196 2216 64-65 0.08348641895751285 2216 66-67 0.015384479836981768 2216 68-69 0.06784699908868674 2216 70-71 0.07647778581483067 2216 72-73 0.1532576337776277 2216 74-75 0.08570308183772113 2216 76-77 0.07380848675791896 2216 78-79 0.021974077181639018 2216 80-81 0.11248844304608241 2216 82-83 0.11104709978223326 2216 84-85 0.0588664790756539 2216 86-87 0.011782459351138641 2216 88-89 0.09144122811577304 2216 90-91 0.1253556248864669 2216 92-93 0.13745036964061796 2216 94-95 0.14086222419847516 2216 96-97 0.10972567996685711 2216 98-99 0.15278225890191877 2216 100 0.14093975990297736 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 26969.0 3 4294.0 4 2783.0 5 2198.0 6 1907.0 7 2078.0 8 2376.0 9 2659.0 10 2961.0 11 3122.0 12 3669.0 13 3915.0 14 4305.0 15 4576.0 16 4366.0 17 4645.0 18 4521.0 19 4442.0 20 4541.0 21 4647.0 22 5377.0 23 7237.0 24 10523.0 25 14644.0 26 15759.0 27 14052.0 28 14625.0 29 18044.0 30 22091.0 31 26927.0 32 35070.0 33 47579.0 34 66720.0 35 102031.0 36 189257.0 37 391910.0 38 629504.0 39 275212.0 40 755.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.828958989290925 19.92351456447284 13.29531668422073 23.952209762015507 2 15.348869626350185 20.164352788295407 40.65436066199699 23.832416923357417 3 18.21301002122165 25.244573941593018 29.878051242910132 26.6643647942752 4 11.956400508505968 16.62359304180539 39.272093590129856 32.14791285955879 5 12.713872881963265 38.02907389294824 36.00402536748638 13.25302785760212 6 30.88207545741936 38.26622094366676 17.88877240176823 12.96293119714565 7 27.270599180078925 32.408273473847984 22.678496293403803 17.642631052669284 8 24.929250935290273 36.87059316197487 20.46801092832763 17.73214497440723 9 24.72558605132014 17.156478641500218 21.278124433954222 36.83981087322542 10-11 23.654284160207318 27.106381711928357 29.07423348110533 20.16510064675899 12-13 24.276224158160673 24.400871954346766 29.087613954599544 22.235289932893018 14-15 21.549510603053893 26.53682346844917 26.05280007507837 25.860865853418563 16-17 21.3893685754759 29.911360646246106 26.53781773367324 22.161453044604755 18-19 21.186838126535285 28.940576102534838 28.46805486493789 21.40453090599199 20-21 22.46295401592722 28.1947344054919 27.956974055511896 21.385337523068987 22-23 22.03653347480727 27.99607263506056 28.011208933645243 21.956184956486926 24-25 21.92103206951039 28.06263180853611 28.00760619929968 22.008729922653824 26-27 21.687859451760144 28.05492842941293 28.083309934359256 22.17390218446767 28-29 21.65779441589341 28.11160947531659 28.105327714899907 22.125268393890092 30-31 21.895001233659066 28.56415203725297 27.796937195401 21.743909533686963 32-33 21.5578984336446 28.184431511183277 28.33124263925991 21.926427415912215 34-35 21.668359410672814 28.027400757521864 28.283683713683576 22.020556118121746 36-37 22.025256552458643 28.402812270524258 27.74580690828084 21.826124268736258 38-39 21.846358221335137 28.529999101929036 27.881319417606026 21.7423232591298 40-41 21.569743316142034 28.203490476676972 28.22861524917291 21.998150958008083 42-43 21.600298668603976 28.02356555826308 28.22019746434391 22.15593830878903 44-45 21.757473739653456 28.222459397711596 28.17614543190674 21.843921430728205 46-47 21.755830810573418 28.422450389645636 27.990261949128747 21.83145685065219 48-49 21.734911373949718 28.261002267676655 27.958838767937728 22.045247590435903 50-51 21.714958285384775 28.175338103552743 27.96153083582094 22.14817277524154 52-53 21.746946938159812 28.058352813448227 28.143941715798938 22.050758532593026 54-55 21.860311659562704 28.543350484076747 27.859977691757802 21.736360164602743 56-57 21.651599969024964 28.24877796171657 27.959711266260946 22.139910802997516 58-59 21.51000697442417 27.866948670508272 28.36267510155796 22.260369253509598 60-61 21.89436740154644 28.295772082842092 27.867794286129406 21.942066229482062 62-63 21.673390122636544 28.36856999368643 27.930656209807143 22.02738367386988 64-65 21.67069912543738 28.017054374629208 28.118101266844516 22.194145233088896 66-67 21.53598882887864 28.104521418546764 27.90517764121046 22.454312111364132 68-69 22.000439909919365 28.24448700046009 27.84251426680335 21.9125588228172 70-71 21.924479081930066 28.021503895455936 27.939727949943638 22.114289072670363 72-73 21.712816536516105 28.136660485996885 28.10722432098183 22.04329865650518 74-75 21.78335999499558 28.218229110701138 27.943439944668853 22.05497094963443 76-77 21.776347170431933 28.22618786668177 27.78433468140133 22.213130281484972 78-79 21.922929750097175 28.265759204088525 27.883109456745824 21.92820158906848 80-81 21.837204585973893 28.038818132011716 28.025625839337547 22.09835144267684 82-83 21.659595967494376 28.138650108980162 28.149496416052234 22.052257507473232 84-85 21.715886942398416 28.03047176448124 27.995511112871196 22.25813018024915 86-87 21.8317614623823 28.177041687581507 27.898720894743086 22.092475955293114 88-89 21.673084364711332 28.037097889454888 27.970945907369465 22.318871838464318 90-91 21.637244362966776 27.916193382240234 28.116647735289607 22.32991451950338 92-93 21.77703385634811 28.167607663552396 27.89511655224899 22.160241927850503 94-95 21.55241094508479 27.872419997990498 28.1258150913562 22.449353965568513 96-97 21.593358372302905 27.92534385027566 28.09092276882661 22.39037500859483 98-99 21.584232922686905 27.99120014370426 28.019134181824455 22.405432751784378 100 21.78062575418206 27.938506442960243 27.920746057955686 22.36012174490201 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 315.0 1 302.0 2 291.0 3 241.0 4 184.0 5 162.0 6 145.0 7 145.0 8 143.0 9 146.0 10 144.5 11 145.5 12 163.5 13 181.0 14 217.5 15 267.0 16 358.5 17 492.0 18 682.5 19 1015.5 20 1487.0 21 2188.0 22 3393.5 23 5148.0 24 7295.5 25 9954.0 26 13427.0 27 17756.0 28 23075.5 29 28820.5 30 34313.0 31 40089.5 32 45579.5 33 50783.5 34 55820.0 35 60179.5 36 63997.5 37 67518.5 38 69447.5 39 70003.5 40 70795.0 41 71171.0 42 70433.5 43 69588.5 44 68354.5 45 66733.0 46 66053.0 47 64570.0 48 62847.0 49 61548.0 50 59503.5 51 57281.5 52 54101.0 53 50859.0 54 48743.0 55 46441.0 56 43282.0 57 39421.5 58 35756.5 59 32439.0 60 29100.0 61 25507.0 62 21829.0 63 18308.5 64 15158.5 65 12495.0 66 10048.0 67 7895.5 68 6149.5 69 4745.5 70 3567.5 71 2646.5 72 2067.5 73 1690.0 74 1411.5 75 1062.0 76 747.5 77 611.0 78 465.5 79 320.5 80 213.0 81 125.0 82 97.0 83 72.0 84 34.0 85 26.0 86 28.0 87 22.5 88 16.0 89 11.5 90 9.0 91 6.0 92 3.0 93 3.0 94 2.5 95 1.5 96 2.5 97 2.5 98 1.0 99 2.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3273989540385342 2 0.11511932405484362 3 0.06532845076731923 4 0.04035734410336323 5 0.04323280487072786 6 0.00973620926493638 7 0.0075165553392514016 8 0.014427750516952354 9 0.017151871243929372 10-11 0.02063269217284445 12-13 0.015461907459601038 14-15 0.018261698206771863 16-17 0.019346301829549748 18-19 0.01864004830774089 20-21 0.02338203623988607 22-23 0.015159227378825814 24-25 0.024542309882857764 26-27 0.018488708267353278 28-29 0.018286921546836463 30-31 0.019270631809355943 32-33 0.02487021330369759 34-35 0.015234897399019618 36-37 0.009660539244742573 38-39 0.01336837023423907 40-41 0.00925696580370894 42-43 0.00812191550080185 44-45 0.008449818921641676 46-47 0.008550712281900083 48-49 0.004338414491111547 50-51 0.006658961777054932 52-53 0.007138205238282371 54-55 0.0032285875282690585 56-57 0.0024971106663955996 58-59 0.0024466639862663958 60-61 0.00312769416801065 62-63 0.002598004026654008 64-65 0.0021692072455557735 66-67 0.0018665271647805494 68-69 0.003379927568656671 70-71 0.0022953239458787834 72-73 0.0017908571445867435 74-75 0.0022196539256849777 76-77 0.0036069376292380884 78-79 0.0030520241478168442 80-81 0.0036826076494318946 82-83 0.0024971106663955996 84-85 0.0031529175080752523 86-87 0.002320547285943386 88-89 0.59670855590829 90-91 0.606217755112645 92-93 0.602913497564182 94-95 0.5878551635456146 96-97 0.5866696665625784 98-99 0.5840212158557951 100 0.5858372963404465 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1982291.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.90299269810919 #Duplication Level Percentage of deduplicated Percentage of total 1 82.11807999284302 59.86653786100982 2 11.781477190806894 17.1780989122867 3 3.0727709830888745 6.7204260162927 4 1.1837479415007612 3.4519507014252717 5 0.5742631973950563 2.093275284324231 6 0.32796822998468395 1.4345919285471127 7 0.20881485034677133 1.0656259257061202 8 0.14305567496703467 0.8343349462035847 9 0.10356404834395048 0.6795116154185085 >10 0.4701809854986049 5.784609360488302 >50 0.014065973285053594 0.6685888481067799 >100 0.0020109319392881356 0.222448600190901 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 2.522334006460202E-4 0.0 10-11 0.0 0.0 0.0 3.0268008077522423E-4 0.0 12-13 0.0 0.0 0.0 3.0268008077522423E-4 0.0 14-15 0.0 0.0 0.0 3.0268008077522423E-4 0.0 16-17 0.0 0.0 0.0 3.2790342083982625E-4 0.0 18-19 0.0 0.0 0.0 5.044668012920404E-4 0.0 20-21 0.0 0.0 0.0 6.053601615504485E-4 0.0 22-23 0.0 0.0 0.0 6.305835016150505E-4 0.0 24-25 0.0 0.0 0.0 7.819235420026626E-4 0.0 26-27 0.0 0.0 0.0 0.001084603622777887 0.0 28-29 0.0 0.0 0.0 0.0018917505048451516 0.0 30-31 0.0 0.0 0.0 0.005296901413566424 0.0 32-33 0.0 0.0 0.0 0.012006309870750562 0.0 34-35 0.0 0.0 0.0 0.02297846279885244 0.0 36-37 0.0 0.0 0.0 0.04270311472937122 0.0 38-39 0.0 0.0 0.0 0.07473675661141578 0.0 40-41 0.0 0.0 0.0 0.11532111077536042 0.0 42-43 0.0 0.0 0.0 0.15371103435368472 0.0 44-45 0.0 0.0 0.0 0.20072734023410288 0.0 46-47 0.0 0.0 0.0 0.25435216121144677 0.0 48-49 0.0 0.0 0.0 0.31920136851753855 0.0 50-51 0.0 0.0 0.0 0.389902390718618 0.0 52-53 0.0 0.0 0.0 0.46241949340434885 0.0 54-55 0.0 0.0 0.0 0.5372319200359583 0.0 56-57 0.0 0.0 0.0 0.6278341575480089 0.0 58-59 0.0 0.0 0.0 0.727895147584285 0.0 60-61 0.0 0.0 0.0 0.8374401134848517 0.0 62-63 0.0 0.0 0.0 0.947943566307873 0.0 64-65 0.0 0.0 0.0 1.0618773933796803 0.0 66-67 0.0 0.0 0.0 1.1950818522608437 0.0 68-69 0.0 0.0 0.0 1.3489442266549159 0.0 70-71 0.0 0.0 0.0 1.5140309873777362 0.0 72-73 0.0 0.0 0.0 1.6943274221595113 0.0 74-75 0.0 0.0 0.0 1.8704115591504982 0.0 76-77 0.0 0.0 0.0 2.058274995951654 0.0 78-79 0.0 0.0 0.0 2.27020149917444 0.0 80-81 0.0 0.0 0.0 2.511437523552294 0.0 82-83 0.0 0.0 0.0 2.76205663043418 0.0 84-85 5.044668012920404E-5 0.0 0.0 3.0094723731278608 0.0 86-87 5.044668012920404E-5 0.0 0.0 3.269171882433003 0.0 88 5.044668012920404E-5 0.0 0.0 3.4880348041735547 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1555 0.0 32.420883 1 TCAACGC 2290 0.0 29.54323 4 GTATCAA 2425 0.0 29.532711 1 CAACGCA 2405 0.0 28.129847 5 ATCAACG 2440 0.0 27.922415 3 TACGTCG 85 6.834185E-4 27.635738 5 GTACATG 5670 0.0 27.505299 1 TACATGG 5585 0.0 26.510363 2 AACGCAG 2595 0.0 26.238705 6 ACATGGG 5760 0.0 24.227509 3 TATCAAC 3025 0.0 22.685888 2 ACGCAGA 3190 0.0 20.903038 7 CATGGGG 3795 0.0 19.931732 4 CGCAGAG 3340 0.0 19.823685 8 TATATCG 150 8.316178E-4 18.792301 5 GAGTACA 3955 0.0 18.10792 1 GTATAGA 880 0.0 17.668623 1 CCTATAC 915 0.0 17.459532 3 AGAGTAC 3375 0.0 16.487558 10-11 GCAGAGT 4020 0.0 16.470427 9 >>END_MODULE