FastQCFastQC Report
Wed 25 May 2016
SRR1033109_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033109_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1982291
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38980.19664115914363733No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30420.15345880095303868No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27700.053.0045621
GTATCAA50950.042.948421
TCAACGC59350.036.51854
ATCAACG60400.036.0393373
CAACGCA61400.035.2992325
TATCAAC63200.034.5170482
AACGCAG63200.034.353536
ACGCAGA72150.030.0920727
CGCAGAG73700.029.330938
GTACATG55250.026.4038871
GCAGAGT83000.025.9312179
TACATGG56100.025.1414972
ACATGGG59150.022.9707853
GAGTACT56300.022.03591312-13
GTGGTAT14050.021.7708531
CAGAGTA81800.021.28484210-11
AGAGTAC76800.020.65133310-11
ACCGATC1708.640956E-519.350168
TACACCG1951.12904E-519.2880425
GTACTTT64400.019.045414-15
Produced by FastQC (version 0.11.5)