##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1033109_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1982291 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.74762080844841 34.0 31.0 34.0 31.0 34.0 2 32.85328188444583 34.0 31.0 34.0 31.0 34.0 3 32.94145561877646 34.0 31.0 34.0 31.0 34.0 4 36.36355156735313 37.0 37.0 37.0 35.0 37.0 5 36.30647518452135 37.0 37.0 37.0 35.0 37.0 6 36.39333629623501 37.0 37.0 37.0 35.0 37.0 7 36.36616773218463 37.0 37.0 37.0 35.0 37.0 8 36.36052325314497 37.0 37.0 37.0 35.0 37.0 9 38.17852373844204 39.0 39.0 39.0 37.0 39.0 10-11 38.15976564490279 39.0 39.0 39.0 37.0 39.0 12-13 38.12828111513395 39.0 39.0 39.0 37.0 39.0 14-15 39.65733764618817 41.0 40.0 41.0 37.0 41.0 16-17 39.670093845959045 41.0 40.0 41.0 37.0 41.0 18-19 39.68834444589619 41.0 40.0 41.0 37.0 41.0 20-21 39.68149984033626 41.0 40.0 41.0 37.0 41.0 22-23 39.61457046417504 41.0 40.0 41.0 37.0 41.0 24-25 39.56726055861627 41.0 40.0 41.0 37.0 41.0 26-27 39.46568667264292 41.0 39.5 41.0 36.5 41.0 28-29 39.37459712020082 41.0 39.0 41.0 36.0 41.0 30-31 39.281937667073095 41.0 39.0 41.0 36.0 41.0 32-33 39.31321914895442 41.0 39.0 41.0 35.5 41.0 34-35 39.255684458033656 41.0 39.0 41.0 35.0 41.0 36-37 39.16529661891216 41.0 39.0 41.0 35.0 41.0 38-39 39.07018066469555 41.0 39.0 41.0 35.0 41.0 40-41 38.95673163021978 41.0 39.0 41.0 35.0 41.0 42-43 38.842972600894626 41.0 39.0 41.0 35.0 41.0 44-45 38.70136498627093 41.0 39.0 41.0 35.0 41.0 46-47 38.4921918628496 41.0 39.0 41.0 35.0 41.0 48-49 38.30588722846444 41.0 38.0 41.0 34.5 41.0 50-51 38.175429591316316 41.0 38.0 41.0 34.0 41.0 52-53 38.04079194225268 40.0 38.0 41.0 34.0 41.0 54-55 37.907143552586376 40.0 38.0 41.0 34.0 41.0 56-57 37.75456277610098 40.0 37.0 41.0 34.0 41.0 58-59 37.57102514212091 40.0 37.0 41.0 33.0 41.0 60-61 37.384588085200406 40.0 36.5 41.0 33.0 41.0 62-63 37.154992380028965 39.5 36.0 41.0 33.0 41.0 64-65 36.910446044501036 39.0 35.0 41.0 33.0 41.0 66-67 36.638702894781844 39.0 35.0 41.0 32.5 41.0 68-69 36.32021282445413 38.5 35.0 41.0 32.0 41.0 70-71 35.9921141749622 37.0 35.0 40.0 32.0 41.0 72-73 35.644567069113464 37.0 35.0 39.5 31.0 41.0 74-75 35.30836844842659 36.0 35.0 39.0 31.0 41.0 76-77 34.974118582993114 36.0 35.0 39.0 31.0 40.5 78-79 34.629338982016264 35.5 35.0 37.5 31.0 39.5 80-81 34.285597321483074 35.0 35.0 37.0 31.0 39.0 82-83 33.95951704366311 35.0 34.5 37.0 30.5 39.0 84-85 33.68344783888945 35.0 34.0 36.0 30.0 38.0 86-87 33.44600666602431 35.0 34.0 36.0 30.0 37.0 88-89 33.22359734267068 35.0 34.0 36.0 30.0 37.0 90-91 33.02193497322038 35.0 34.0 35.0 30.0 36.5 92-93 32.85493754448767 35.0 34.0 35.0 30.0 36.0 94-95 32.70448460897013 35.0 34.0 35.0 29.0 36.0 96-97 32.577487109612065 35.0 34.0 35.0 29.0 36.0 98-99 32.471458025083095 35.0 34.0 35.0 29.0 36.0 100 32.37643363159092 35.0 34.0 35.0 29.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.10446855046106407 1101 2 0.15704512289012484 1101 3 0.14763671808871237 1101 4 0.07182258468220937 1101 5 0.032838208119450485 1101 6 0.049643642523932385 1101 7 0.08698274833518838 1101 8 0.11990433118815957 1101 9 0.1051778234505889 1101 10-11 0.15544301044489828 1101 12-13 0.16478304167764435 1101 14-15 0.21244722960759077 1101 16-17 0.1902415290346795 1101 18-19 0.1992619487804106 1101 20-21 0.1446693517491866 1101 22-23 0.19392743296678816 1101 24-25 0.16991757079427572 1101 26-27 0.15816484499058703 1101 28-29 0.13911834377564247 1101 30-31 0.14650766703868356 1101 32-33 0.09386099255566904 1101 34-35 0.17117721181202228 1101 36-37 0.20616409184373197 1101 38-39 0.20185656650547656 1101 40-41 0.2312374857556847 1101 42-43 0.12520854577449825 1101 44-45 0.09919112638954175 1101 46-47 0.16755505554037597 1101 48-49 0.10103320304508401 1101 50-51 0.08219262244620751 1101 52-53 0.18947405777102233 1101 54-55 0.2111398139702061 1101 56-57 0.1557547944599733 1101 58-59 0.0260869174145526 1101 60-61 0.11212021360685043 1101 62-63 0.08797909423727646 1101 64-65 0.1445005878585519 1101 66-67 0.14347660996102718 1101 68-69 0.06355172271095455 1101 70-71 0.12186714022096368 1101 72-73 0.18932335628689145 1101 74-75 0.16661725698068608 1101 76-77 0.15511072773227141 1101 78-79 0.20294236363340445 1101 80-81 0.057615504044129295 1101 82-83 0.10896796571061884 1101 84-85 0.11914050749129501 1101 86-87 0.11769781501872956 1101 88-89 0.11863159570240356 1101 90-91 0.07238144557766901 1101 92-93 0.030791511264112614 1101 94-95 0.04313595202258114 1101 96-97 0.06725455516138368 1101 98-99 0.0866273872673986 1101 100 0.055287556931332915 1102 1 0.09553510088245076 1102 2 0.11428042270607364 1102 3 0.15141856445120538 1102 4 0.109561614034142 1102 5 0.06320341203304736 1102 6 0.09344680052063836 1102 7 0.08090893258395226 1102 8 0.15240263594452585 1102 9 0.1397801677303505 1102 10-11 0.13753736286183482 1102 12-13 0.13749930632938856 1102 14-15 0.13902283747351873 1102 16-17 0.16993638518115262 1102 18-19 0.18829130071299005 1102 20-21 0.19423924422230243 1102 22-23 0.22009811978264082 1102 24-25 0.21550703122245096 1102 26-27 0.24774636199146016 1102 28-29 0.21244586484510108 1102 30-31 0.20993690272598542 1102 32-33 0.19575482186886006 1102 34-35 0.23818486462613464 1102 36-37 0.20481467791367436 1102 38-39 0.165914495402113 1102 40-41 0.15252296478310257 1102 42-43 0.12207186070232723 1102 44-45 0.10749975760140273 1102 46-47 0.19277931205495236 1102 48-49 0.12043066502319277 1102 50-51 0.04520531255567306 1102 52-53 0.1363024991155939 1102 54-55 0.1270208563028561 1102 56-57 0.09046950917468877 1102 58-59 0.11362445495208817 1102 60-61 0.05427365737319434 1102 62-63 0.03419821658543043 1102 64-65 0.012315176640882441 1102 66-67 0.0507894066286525 1102 68-69 0.13125394105833266 1102 70-71 0.05195723031105359 1102 72-73 0.01711760214564606 1102 74-75 -0.00825923241193749 1102 76-77 0.021309507156850316 1102 78-79 0.03634447542260233 1102 80-81 0.045088137968370745 1102 82-83 -0.03142048725847246 1102 84-85 0.029061073218315414 1102 86-87 -0.003723283499148522 1102 88-89 0.03821795625650992 1102 90-91 0.008130743262448448 1102 92-93 0.018889932265757636 1102 94-95 -0.02287005398342501 1102 96-97 -0.034228540837844434 1102 98-99 -0.056711758329811346 1102 100 -0.07880137070598181 1103 1 0.13885832485083682 1103 2 0.19169977754478396 1103 3 0.17641626686826584 1103 4 0.08793446579409192 1103 5 0.10028180556304989 1103 6 0.10557803845833291 1103 7 0.1613128476652932 1103 8 0.13695368823751863 1103 9 0.15830787658064338 1103 10-11 0.15886008136197205 1103 12-13 0.15492991932452327 1103 14-15 0.17028949494985568 1103 16-17 0.23656169535484395 1103 18-19 0.16794505496351775 1103 20-21 0.12403544861528815 1103 22-23 0.14212389866325026 1103 24-25 0.16693612781283917 1103 26-27 0.23257771940345862 1103 28-29 0.20520962736692638 1103 30-31 0.2419950775260986 1103 32-33 0.2586844698791495 1103 34-35 0.3172217178565333 1103 36-37 0.2767899099695512 1103 38-39 0.32172393387284615 1103 40-41 0.3516613711795742 1103 42-43 0.35510421067016296 1103 44-45 0.42233869703711946 1103 46-47 0.3497938327791559 1103 48-49 0.4001648346767155 1103 50-51 0.3933649486185331 1103 52-53 0.46120944700641076 1103 54-55 0.45995670778710007 1103 56-57 0.49545526416044794 1103 58-59 0.5054691842968211 1103 60-61 0.4449684464550785 1103 62-63 0.4355905518487333 1103 64-65 0.3594742153321846 1103 66-67 0.4253092117936248 1103 68-69 0.42895635811558464 1103 70-71 0.44985609070992183 1103 72-73 0.45356875553228804 1103 74-75 0.42862156898500103 1103 76-77 0.3685822762038171 1103 78-79 0.3301938458848852 1103 80-81 0.2976464965751191 1103 82-83 0.31512739437005166 1103 84-85 0.3219155902663857 1103 86-87 0.31239770721862214 1103 88-89 0.3279339750047896 1103 90-91 0.4242793049755278 1103 92-93 0.35552374349634164 1103 94-95 0.4499575813442078 1103 96-97 0.4994827448895691 1103 98-99 0.453217246357255 1103 100 0.5368980135417942 1104 1 0.02735056080069853 1104 2 0.06385089787992371 1104 3 0.03065197824682997 1104 4 0.05837532213082852 1104 5 0.03635243836492208 1104 6 0.024564755265870986 1104 7 0.054220619205025855 1104 8 0.07055004435203216 1104 9 0.041017848878993846 1104 10-11 0.07283067829261114 1104 12-13 0.1268236710306212 1104 14-15 0.12691580402773894 1104 16-17 0.10957609016100633 1104 18-19 0.0978312894805029 1104 20-21 0.10395218352596913 1104 22-23 0.15465338894697567 1104 24-25 0.08035393590376572 1104 26-27 0.08933427107526626 1104 28-29 0.11863451854605955 1104 30-31 0.13854589689773888 1104 32-33 0.047485319061358666 1104 34-35 0.0830959655709762 1104 36-37 0.022635339985683345 1104 38-39 0.07436223024600963 1104 40-41 -0.014898051372583154 1104 42-43 -0.012313805355603336 1104 44-45 0.025249131818014803 1104 46-47 -0.0598039118670215 1104 48-49 -0.0848357097971899 1104 50-51 -0.04922840771574499 1104 52-53 -0.06779432095014215 1104 54-55 -0.08666393235654368 1104 56-57 0.06827803371444219 1104 58-59 -0.0059746841385717175 1104 60-61 0.05901380384184307 1104 62-63 -0.016936004188721654 1104 64-65 -0.031228375279610532 1104 66-67 -0.05353218714462571 1104 68-69 -0.02699028245574908 1104 70-71 -0.08613800778417868 1104 72-73 0.007317815637279068 1104 74-75 -0.02562365220937579 1104 76-77 -0.056521395570563016 1104 78-79 -0.0788111366673192 1104 80-81 -0.02943296261693007 1104 82-83 -0.03549920640787718 1104 84-85 -0.04007333104456734 1104 86-87 -0.08751893407211497 1104 88-89 -0.05428597077447961 1104 90-91 -0.04923961512329811 1104 92-93 0.017651259286090237 1104 94-95 -0.1148182456894844 1104 96-97 -0.0619046559348746 1104 98-99 -0.0594992374548724 1104 100 0.00721790305054526 1105 1 0.04359752590529098 1105 2 0.053919725230443305 1105 3 0.08551937044424562 1105 4 0.04870271967902795 1105 5 0.015123010834145134 1105 6 0.025212537929064638 1105 7 -0.02057024990357803 1105 8 7.178625838122343E-5 1105 9 0.06683651387078982 1105 10-11 0.014518319121030743 1105 12-13 0.03221229283258964 1105 14-15 0.07567405930320348 1105 16-17 0.06339947715680694 1105 18-19 0.07386982175061263 1105 20-21 0.09448852609241953 1105 22-23 0.09111905652483898 1105 24-25 0.04706659289724513 1105 26-27 -0.04654436780460003 1105 28-29 -0.004630768694042331 1105 30-31 0.10081087505734843 1105 32-33 0.08084541812760904 1105 34-35 0.08567535376155888 1105 36-37 0.0413894092799012 1105 38-39 0.043550481505427285 1105 40-41 0.18726596049449995 1105 42-43 0.05836649389662085 1105 44-45 0.18171696850597385 1105 46-47 0.2803858392411698 1105 48-49 0.17528661715520144 1105 50-51 0.2351698371225055 1105 52-53 0.2368892928628199 1105 54-55 0.2651160419894225 1105 56-57 0.21934475084419347 1105 58-59 0.16583075541836223 1105 60-61 0.20387194486728077 1105 62-63 0.2551213849414893 1105 64-65 0.2246807680387306 1105 66-67 0.206829768646827 1105 68-69 0.2153591493802054 1105 70-71 0.2479995366911325 1105 72-73 0.22558328048949505 1105 74-75 0.14528794404441214 1105 76-77 0.2177254611965438 1105 78-79 0.23426125673325515 1105 80-81 0.2673752945408623 1105 82-83 0.22298661076304427 1105 84-85 0.24771612507408491 1105 86-87 0.1546631529257212 1105 88-89 0.0723394898811307 1105 90-91 0.0391851377037753 1105 92-93 0.17305141702453142 1105 94-95 0.1991474599481151 1105 96-97 0.09525403878011218 1105 98-99 0.009783767803511978 1105 100 0.03757199080225604 1106 1 -0.015110630842258388 1106 2 0.045295415623179736 1106 3 0.014919557957760787 1106 4 0.06360741991532137 1106 5 -0.007231211432724649 1106 6 -0.020674540380454687 1106 7 0.015166369622257037 1106 8 0.024491756810064658 1106 9 0.009472038089633372 1106 10-11 -0.004914601809268504 1106 12-13 0.05395610067829182 1106 14-15 0.03388120336914824 1106 16-17 0.0011505547712928887 1106 18-19 0.020668799928635906 1106 20-21 0.013188361475094723 1106 22-23 0.04768313827421622 1106 24-25 0.011714593280956365 1106 26-27 -0.010011189133720677 1106 28-29 -0.023907918560965413 1106 30-31 0.09198034130446331 1106 32-33 -0.03590488074468823 1106 34-35 0.07097331428054332 1106 36-37 0.09571244656450517 1106 38-39 0.09285813173117674 1106 40-41 0.05931315426239081 1106 42-43 0.11708353083913892 1106 44-45 0.0783277431123679 1106 46-47 0.11080301835730211 1106 48-49 0.0650508745627576 1106 50-51 0.05023202789940484 1106 52-53 0.06535769339603803 1106 54-55 0.008979205085459796 1106 56-57 0.020715604075952854 1106 58-59 0.13303574289785303 1106 60-61 0.12694236334279196 1106 62-63 0.033917990693410616 1106 64-65 0.003305878560396991 1106 66-67 0.019682431166842207 1106 68-69 0.05470544938192745 1106 70-71 0.030714966827410706 1106 72-73 0.10672107894323091 1106 74-75 0.027200751615900742 1106 76-77 -0.041520631743914294 1106 78-79 -0.06522196116885937 1106 80-81 -0.003752216461528235 1106 82-83 -0.012391271510679758 1106 84-85 0.01470834986948688 1106 86-87 0.050283305018012925 1106 88-89 0.046343199879522956 1106 90-91 0.03963321989841262 1106 92-93 0.07581797672161628 1106 94-95 0.012040055064616695 1106 96-97 0.08751938896070044 1106 98-99 0.15932172616863483 1106 100 0.1354587758956569 1107 1 -0.004697275482762109 1107 2 -0.03314196006709125 1107 3 -0.004604560517982748 1107 4 0.05912749273367979 1107 5 -0.036981080717517045 1107 6 -0.053263665570014496 1107 7 -0.01556950922689282 1107 8 -0.06095125917627087 1107 9 -0.036913187009773196 1107 10-11 -0.06073108113194081 1107 12-13 0.02792575216810178 1107 14-15 0.02830007656063316 1107 16-17 0.042856088584407814 1107 18-19 0.023390694421927094 1107 20-21 0.02590052398232956 1107 22-23 0.007713163687689928 1107 24-25 0.047527086006937225 1107 26-27 0.0795935428436465 1107 28-29 0.08533916184322266 1107 30-31 0.036814398131269854 1107 32-33 0.0767943188092417 1107 34-35 0.05057967911233163 1107 36-37 0.08690198100990898 1107 38-39 0.08951935699734292 1107 40-41 0.1107688048940787 1107 42-43 0.09889055784550038 1107 44-45 0.0654216791426947 1107 46-47 0.07298647910539557 1107 48-49 0.01272668434563684 1107 50-51 0.047477671739116545 1107 52-53 0.005261593833608913 1107 54-55 -0.03780229119980305 1107 56-57 -0.0369808291793845 1107 58-59 0.010229999593001082 1107 60-61 0.03425865853507304 1107 62-63 0.010767285178708619 1107 64-65 -0.05298786326447669 1107 66-67 0.03361749741036135 1107 68-69 0.0795435101762223 1107 70-71 0.0029722925245536658 1107 72-73 0.027094998262853665 1107 74-75 0.10448872373230955 1107 76-77 0.07787413947406918 1107 78-79 0.028177854527037027 1107 80-81 0.09874348861022497 1107 82-83 0.14317936768233608 1107 84-85 0.06161524155739073 1107 86-87 0.07315738067475763 1107 88-89 0.11107252386042887 1107 90-91 0.14614505201318195 1107 92-93 0.04948157657499763 1107 94-95 0.02502341905739769 1107 96-97 0.03301625005863684 1107 98-99 0.11827715098003466 1107 100 0.17407903996643626 1108 1 0.020942166934680984 1108 2 0.019636285481290372 1108 3 -0.029574779122782502 1108 4 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-0.04209738875170643 2214 18-19 -0.049160463455230285 2214 20-21 -0.11579557891029424 2214 22-23 -0.09716947657370412 2214 24-25 -0.11206106752677414 2214 26-27 -0.11855356752548829 2214 28-29 -0.03858450723460294 2214 30-31 0.02813279411128633 2214 32-33 -0.023636331167331548 2214 34-35 -0.0690509660815195 2214 36-37 -0.04017759255242481 2214 38-39 -0.025751178923279383 2214 40-41 0.06677078619171084 2214 42-43 0.09090215318015993 2214 44-45 0.00944737485590963 2214 46-47 0.1072364370116361 2214 48-49 0.1371662591781373 2214 50-51 0.1609786038780996 2214 52-53 0.015208166617973973 2214 54-55 0.10976076955613934 2214 56-57 0.0976064275956503 2214 58-59 -3.4175609583542155E-4 2214 60-61 -0.024601542570160007 2214 62-63 -0.04813853791149114 2214 64-65 0.0939314913750593 2214 66-67 -0.04253696422375697 2214 68-69 0.03060312610476501 2214 70-71 0.09618550527863334 2214 72-73 0.01278949228220938 2214 74-75 0.06621898381976621 2214 76-77 0.0959994943797895 2214 78-79 -0.04467589814829154 2214 80-81 -0.010537042396357776 2214 82-83 -0.0185890855040185 2214 84-85 0.0024281890669186623 2214 86-87 0.05058390673750068 2214 88-89 -0.026542396008544245 2214 90-91 0.06418322041446345 2214 92-93 0.04496372598107001 2214 94-95 -0.003960213283697556 2214 96-97 -0.12880483257115571 2214 98-99 -0.17270770957742343 2214 100 -0.3252011029347557 2215 1 -0.03434605361670151 2215 2 -0.08052244467744174 2215 3 -0.09279950761067113 2215 4 -0.07216824825599133 2215 5 -0.09642684640876098 2215 6 -0.024606396989263146 2215 7 -0.07491072408459587 2215 8 -0.054416832080370625 2215 9 -0.03831830425606597 2215 10-11 -0.09401328795876651 2215 12-13 -0.08806190853572815 2215 14-15 -0.1702207014813908 2215 16-17 -0.10566727450569857 2215 18-19 -0.16467040462563176 2215 20-21 -0.105359888016892 2215 22-23 -0.20572248010417837 2215 24-25 -0.14751881295789104 2215 26-27 -0.1466320571747346 2215 28-29 -0.09886332210075466 2215 30-31 -0.14308139570827905 2215 32-33 -0.18356511776517692 2215 34-35 -0.18177363377039057 2215 36-37 -0.14117192838702408 2215 38-39 -0.1767897113513257 2215 40-41 -0.23802598824462962 2215 42-43 -0.1611388542571106 2215 44-45 -0.07426311732785251 2215 46-47 -0.14526539649060055 2215 48-49 -0.1799277579158769 2215 50-51 -0.040137075672966205 2215 52-53 -0.10116684629093697 2215 54-55 -0.19083426537229542 2215 56-57 -0.3355538826381803 2215 58-59 -0.26620010645142145 2215 60-61 -0.18695912889354815 2215 62-63 -0.1702349092398876 2215 64-65 -0.13511455359869728 2215 66-67 -0.18260051980031733 2215 68-69 -0.16781227286357137 2215 70-71 -0.11310124922110987 2215 72-73 -0.10878317129332515 2215 74-75 -0.10748579343815123 2215 76-77 -0.2358636677158117 2215 78-79 -0.19713034064982793 2215 80-81 -0.17905353341438257 2215 82-83 -0.06702697660011125 2215 84-85 -0.18461087547061084 2215 86-87 -0.24688966720033534 2215 88-89 -0.2554106043924236 2215 90-91 -0.21275210902957298 2215 92-93 -0.1529314221956639 2215 94-95 -0.1449460773752378 2215 96-97 -0.10787030654242358 2215 98-99 -0.17269930784877374 2215 100 -0.2033816385799625 2216 1 -0.08298279699028654 2216 2 -0.09993081408580906 2216 3 -0.1610610706090725 2216 4 -0.07408592122629898 2216 5 -0.09989764461639794 2216 6 -0.11436868148839352 2216 7 -0.16078449943205442 2216 8 -0.16037490909108243 2216 9 -0.17422625595349217 2216 10-11 -0.24369430869241882 2216 12-13 -0.22384454694994105 2216 14-15 -0.31115637399601326 2216 16-17 -0.2951732363800872 2216 18-19 -0.20159605207759057 2216 20-21 -0.2640463769665402 2216 22-23 -0.2407779217385695 2216 24-25 -0.323458662581956 2216 26-27 -0.366253079427338 2216 28-29 -0.3646755800182788 2216 30-31 -0.22780456727355158 2216 32-33 -0.24205414335946074 2216 34-35 -0.33956963893481884 2216 36-37 -0.41380236812273097 2216 38-39 -0.34628352163461074 2216 40-41 -0.4616114670932632 2216 42-43 -0.3854876049216571 2216 44-45 -0.2200499328515093 2216 46-47 -0.2788284708431519 2216 48-49 -0.279783457771579 2216 50-51 -0.2827284024748167 2216 52-53 -0.31804046974350797 2216 54-55 -0.2508593280289375 2216 56-57 -0.24214232343714315 2216 58-59 -0.19149820017056385 2216 60-61 -0.21905641756978156 2216 62-63 -0.14715448089629746 2216 64-65 -0.2269786836207146 2216 66-67 -0.1879738014893917 2216 68-69 -0.1777461985869664 2216 70-71 -0.2500629717091485 2216 72-73 -0.15552122855770278 2216 74-75 -0.2153881250246954 2216 76-77 -0.18790475528322048 2216 78-79 -0.18513064443961014 2216 80-81 -0.25241686598824487 2216 82-83 -0.1793304625878065 2216 84-85 -0.1946530263022339 2216 86-87 -0.17860342128251006 2216 88-89 -0.19126349419268251 2216 90-91 -0.20537761218138684 2216 92-93 -0.17193537146277293 2216 94-95 -0.16558289669626447 2216 96-97 -0.19229538438855798 2216 98-99 -0.11990823926822713 2216 100 -0.15365125200747087 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 20.0 3 7.0 4 13.0 5 17.0 6 69.0 7 130.0 8 243.0 9 479.0 10 776.0 11 1273.0 12 1973.0 13 3085.0 14 3734.0 15 4975.0 16 6218.0 17 8662.0 18 10142.0 19 5647.0 20 3659.0 21 3453.0 22 3629.0 23 3954.0 24 4511.0 25 5405.0 26 6873.0 27 8772.0 28 11095.0 29 14380.0 30 18030.0 31 23720.0 32 30305.0 33 39790.0 34 55470.0 35 86507.0 36 161328.0 37 375264.0 38 704472.0 39 372382.0 40 1829.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.88204235930429 19.9648276499306 13.547565226128523 23.60556476463659 2 15.681824258435938 20.291807898346487 40.19794547948481 23.828422363732766 3 18.321427076044838 25.3563175134226 30.182248721302773 26.140006689229782 4 12.205958171525198 16.79818149348782 39.036748481298865 31.959111853688114 5 12.516792579471419 37.82264209979157 35.962160552704226 13.698404768032782 6 30.8030534474374 38.38758724784138 17.527105096948773 13.282254207772448 7 27.03431220458676 32.67960897617321 22.554398412657573 17.731680406582445 8 25.12967378001717 36.211850786114184 20.329700819560486 18.328774614308156 9 25.079440989684148 16.250254125407544 21.452371475602195 37.217933409306106 10-11 23.803174718481593 26.674506100532412 28.967843187108215 20.554475993877773 12-13 24.830839669856747 23.99821721432423 29.30838610476464 21.86255701105438 14-15 21.829396132146286 25.884382087769286 26.830384888573207 25.455836891511225 16-17 20.943468945780413 29.564428229760413 27.21013211481059 22.281970709648583 18-19 20.737067283596712 27.958720438523454 30.097334469730313 21.20687780814952 20-21 21.944633759624598 27.24975798205208 29.804125630394328 21.001482627928997 22-23 21.476850837076697 27.096803159787026 29.924296979420976 21.5020490237153 24-25 21.354236424984126 27.184562504839732 29.867721204334075 21.593479865842067 26-27 21.171046302417718 27.15602860672015 29.962797542100272 21.710127548761857 28-29 21.184919662936228 27.22078786953851 29.94522272266906 21.649069744856195 30-31 21.400635257711357 27.654754491163015 29.598048606197754 21.34656164492788 32-33 21.098227103343703 27.356297479292657 30.06342766748159 21.48204774988205 34-35 21.24387144826584 27.143630645887285 30.026472113954227 21.58602579189265 36-37 21.623712985359276 27.549284943508734 29.418879570322744 21.408122500809252 38-39 21.479168180460352 27.748696119036666 29.42454142143209 21.347594279070893 40-41 21.30608912291308 27.45882043633697 29.633945136303897 21.601145304446053 42-43 21.355757564595475 27.33956684644299 29.51398831727187 21.790687271689656 44-45 21.507064245389337 27.598807475755567 29.283221480573797 21.6109067982813 46-47 21.626367106052843 27.854187463530593 28.965203551315998 21.554241879100562 48-49 21.659482677070606 27.75329018482954 28.75169109862192 21.83553603947793 50-51 21.649259068200536 27.787799251563655 28.607345876221697 21.95559580401411 52-53 21.635260073030924 27.751927933672825 28.676039540423798 21.936772452872454 54-55 21.75436817506148 28.121489864956047 28.463558161069496 21.66058379891298 56-57 21.563266101967248 27.853768058219146 28.554038384459556 22.028927455354047 58-59 21.404109977760385 27.506900536177277 28.954657366907238 22.1343321191551 60-61 21.71630863993563 27.87195536447344 28.520956324228063 21.890779671362864 62-63 21.47498479642853 27.82592837635946 28.669458591424103 22.029628235787907 64-65 21.52769317190912 27.501342553249984 28.8033606586116 22.167603616229293 66-67 21.439340071882 27.67199732227063 28.534112168509285 22.35455043733808 68-69 21.88869878692075 27.818467670516046 28.498067492840907 21.7947660497223 70-71 21.815385228507818 27.619748830200706 28.496464558947014 22.06840138234446 72-73 21.66728085794071 27.763387793307864 28.568419772872822 22.0009115758786 74-75 21.769259310546964 27.77203944041247 28.43221308376232 22.02648816527824 76-77 21.77418114008415 27.830529790516845 28.25049999155015 22.144789077848856 78-79 21.863330255242737 27.95527595742196 28.300180170454414 21.881213616880885 80-81 21.78442410536145 27.716126018460553 28.415169191451668 22.08428068472633 82-83 21.569501862749302 27.838630671169888 28.514567784148092 22.07729968193272 84-85 21.59842968940358 27.77280035191746 28.343379830226056 22.2853901284529 86-87 21.750144277723518 27.832080908525906 28.290868935290938 22.126905878459638 88-89 21.531828576127317 27.78469457814216 28.312038948872793 22.371437896857728 90-91 21.477497501628168 27.667330376821564 28.387658522386474 22.467513599163798 92-93 21.563761324649107 27.866266859911082 28.243961154038434 22.32601066140138 94-95 21.34661359003295 27.61610177315036 28.408190321199058 22.629094315617635 96-97 21.333068656418256 27.608887897891883 28.402666409724908 22.655377035964953 98-99 21.30259886162022 27.72501615554931 28.305531327136123 22.666853655694347 100 21.467231602221872 27.593779117193186 28.213920156021494 22.725069124563447 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 41.0 1 37.0 2 103.5 3 209.5 4 163.5 5 107.0 6 258.0 7 576.0 8 1010.5 9 1128.5 10 880.5 11 685.5 12 699.0 13 858.0 14 980.0 15 1095.0 16 1278.0 17 1472.0 18 1735.0 19 2136.5 20 2644.5 21 3348.0 22 4396.5 23 5970.5 24 8030.0 25 10529.0 26 13804.0 27 17835.0 28 22725.5 29 28205.0 30 33556.0 31 39334.5 32 45355.0 33 50410.5 34 55031.0 35 59681.0 36 63435.5 37 66445.5 38 68872.0 39 69762.5 40 70480.0 41 71103.0 42 70037.5 43 69132.0 44 68558.0 45 67236.5 46 66461.5 47 64865.0 48 62527.5 49 60906.5 50 58693.5 51 56710.5 52 53745.0 53 50576.0 54 48383.0 55 45859.0 56 42754.5 57 39077.0 58 35567.0 59 32363.0 60 29071.5 61 25231.5 62 21495.0 63 18082.5 64 14855.0 65 12051.5 66 9753.5 67 7618.5 68 5856.5 69 4492.5 70 3331.0 71 2519.0 72 1991.5 73 1607.0 74 1272.5 75 954.5 76 688.5 77 521.5 78 401.5 79 284.0 80 177.5 81 89.0 82 42.0 83 26.0 84 15.0 85 7.5 86 4.5 87 3.0 88 1.5 89 1.5 90 2.0 91 0.5 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08868526366714069 2 0.001412507043617713 3 0.0 4 2.522334006460202E-4 5 0.03838992357832428 6 3.5312676090442827E-4 7 0.005549134814212445 8 4.5402012116283635E-4 9 1.0089336025840808E-4 10-11 2.522334006460202E-4 12-13 0.0 14-15 1.5134004038761212E-4 16-17 0.0 18-19 1.261167003230101E-4 20-21 0.0 22-23 2.774567407106222E-4 24-25 2.522334006460202E-5 26-27 0.001488177063811519 28-29 0.0087524990024169 30-31 0.036977416534706556 32-33 0.025526020145377245 34-35 0.02941041451532595 36-37 0.026459283727767516 38-39 0.025980040266540078 40-41 0.032008418541979965 42-43 0.024037843081565723 44-45 0.022423549317431193 46-47 0.016218607661539097 48-49 0.011173939648618694 50-51 0.00799579880047884 52-53 0.005902261575116872 54-55 0.004262744470917741 56-57 0.002850237427300028 58-59 8.828169022610706E-4 60-61 6.810301817442545E-4 62-63 0.001336837023423907 64-65 0.0030520241478168442 66-67 7.314768618734586E-4 68-69 0.0010846036227778867 70-71 3.2790342083982625E-4 72-73 4.7924346122743837E-4 74-75 4.7924346122743837E-4 76-77 3.783501009690303E-4 78-79 7.567002019380606E-5 80-81 5.044668012920404E-4 82-83 3.0268008077522423E-4 84-85 4.035734410336323E-4 86-87 1.5134004038761212E-4 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1982291.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.7167422665678 #Duplication Level Percentage of deduplicated Percentage of total 1 80.70937893504646 57.075043486444365 2 12.709353590451949 17.97528164461337 3 3.332616145656692 7.070152711374206 4 1.2412133279368278 3.5109825203815017 5 0.6261862438624324 2.2140925609044904 6 0.3493953982972396 1.4824862598306419 7 0.22386054417861245 1.1081481884432787 8 0.1546797046570144 0.8750755846479132 9 0.11654857872531948 0.7417742222927886 >10 0.5158972646352442 6.1677467312815395 >50 0.01637045926945681 0.7598165776257448 >100 0.004143003064043985 0.5161214024293688 >500 1.4272168747681414E-4 0.07075061765192019 >1k 2.140825312151769E-4 0.43252749207882674 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3898 0.19664115914363733 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3042 0.15345880095303868 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.0089336025840808E-4 0.0 10-11 0.0 0.0 0.0 1.7656338045221414E-4 0.0 12-13 0.0 0.0 0.0 2.0178672051681616E-4 0.0 14-15 0.0 0.0 0.0 2.0178672051681616E-4 0.0 16-17 0.0 0.0 0.0 2.774567407106222E-4 0.0 18-19 0.0 0.0 0.0 4.2879678109823433E-4 0.0 20-21 0.0 0.0 0.0 4.7924346122743837E-4 0.0 22-23 0.0 0.0 0.0 5.801368214858464E-4 0.0 24-25 0.0 0.0 0.0 7.819235420026626E-4 0.0 26-27 0.0 0.0 0.0 0.0011098269628424889 0.0 28-29 0.0 0.0 0.0 0.0019421971849743554 0.0 30-31 0.0 0.0 0.0 0.0053725714337602305 0.0 32-33 0.0 0.0 0.0 0.01210720323100897 0.0 34-35 0.0 0.0 0.0 0.02307935615911085 0.0 36-37 0.0 0.0 0.0 0.04295534813001724 0.0 38-39 0.0 0.0 0.0 0.0750646600322556 0.0 40-41 0.0 0.0 0.0 0.11572468421639406 0.0 42-43 0.0 0.0 0.0 0.15424072449504134 0.0 44-45 0.0 0.0 0.0 0.2014083704158471 0.0 46-47 0.0 0.0 0.0 0.255159308093514 0.0 48-49 0.0 0.0 0.0 0.3206138755611563 0.0 50-51 0.0 0.0 0.0 0.39184458790359233 0.0 52-53 0.0 0.0 0.0 0.4648913807306798 0.0 54-55 0.0 0.0 0.0 0.5400064874430646 0.0 56-57 0.0 0.0 0.0 0.6311636384365362 0.0 58-59 0.0 0.0 0.0 0.7317543186141691 0.0 60-61 0.0 0.0 0.0 0.8417533046358985 0.0 62-63 0.0 0.0 0.0 0.9533413610816979 0.0 64-65 0.0 0.0 0.0 1.0685615784968 0.0 66-67 0.0 0.0 0.0 1.2028254176606765 0.0 68-69 0.0 0.0 0.0 1.357999405738108 0.0 70-71 0.0 0.0 0.0 1.5240698767234477 0.0 72-73 0.0 0.0 0.0 1.7042149714648354 0.0 74-75 0.0 0.0 0.0 1.8807279052369203 0.0 76-77 0.0 0.0 0.0 2.070104742441952 0.0 78-79 0.0 0.0 0.0 2.2853355032132017 0.0 80-81 0.0 0.0 0.0 2.5306324853414557 0.0 82-83 0.0 0.0 0.0 2.784076606310577 0.0 84-85 0.0 0.0 0.0 3.0358055401553052 0.0 86-87 0.0 0.0 0.0 3.299843463951559 0.0 88 0.0 0.0 0.0 3.5225403333819303 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2770 0.0 53.004562 1 GTATCAA 5095 0.0 42.94842 1 TCAACGC 5935 0.0 36.5185 4 ATCAACG 6040 0.0 36.039337 3 CAACGCA 6140 0.0 35.299232 5 TATCAAC 6320 0.0 34.517048 2 AACGCAG 6320 0.0 34.35353 6 ACGCAGA 7215 0.0 30.092072 7 CGCAGAG 7370 0.0 29.33093 8 GTACATG 5525 0.0 26.403887 1 GCAGAGT 8300 0.0 25.931217 9 TACATGG 5610 0.0 25.141497 2 ACATGGG 5915 0.0 22.970785 3 GAGTACT 5630 0.0 22.035913 12-13 GTGGTAT 1405 0.0 21.770853 1 CAGAGTA 8180 0.0 21.284842 10-11 AGAGTAC 7680 0.0 20.651333 10-11 ACCGATC 170 8.640956E-5 19.35016 8 TACACCG 195 1.12904E-5 19.288042 5 GTACTTT 6440 0.0 19.0454 14-15 >>END_MODULE