FastQCFastQC Report
Wed 25 May 2016
SRR1033108_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033108_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1842402
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA24050.032.7099951
TCAACGC22950.032.3406684
GGTATCA15100.032.1321221
ATCAACG23750.031.2538493
CAACGCA24250.030.6069455
AACGCAG25950.028.7750746
TATCAAC28700.026.531112
CGCGTAT459.5654256E-426.0906112-13
GTACATG50800.026.0568371
TACATGG51000.025.0680792
CCGGTCG1551.5582282E-624.2390179
ACGCAGA30650.023.9029147
ACATGGG52850.023.2898313
CGCAGAG31450.023.1455638
GTATAGA8450.020.6264321
CATGGGG36800.019.1477624
GCAGAGT38400.018.222669
TATAACG1550.001033254918.1945742
GTACCGT1550.001039110918.1792626
GTATTAG9600.017.1741771
Produced by FastQC (version 0.11.5)