##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033108_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1842402
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.473675126275374	33.0	31.0	34.0	30.0	34.0
2	31.56387802444852	33.0	31.0	34.0	30.0	34.0
3	31.644692634940693	34.0	31.0	34.0	30.0	34.0
4	35.009723176592296	37.0	35.0	37.0	33.0	37.0
5	35.08451195775949	37.0	35.0	37.0	33.0	37.0
6	35.17860325813802	37.0	35.0	37.0	33.0	37.0
7	35.076751979209746	37.0	35.0	37.0	33.0	37.0
8	35.09586290071331	37.0	35.0	37.0	33.0	37.0
9	36.71906891112798	39.0	38.0	39.0	33.0	39.0
10-11	36.72133144666582	39.0	37.5	39.0	33.0	39.0
12-13	36.70029776346313	39.0	38.0	39.0	33.0	39.0
14-15	38.14238912028971	41.0	39.0	41.0	33.0	41.0
16-17	38.15517188973959	41.0	39.0	41.0	33.0	41.0
18-19	38.13751721936906	41.0	39.0	41.0	33.5	41.0
20-21	38.15594207995866	41.0	39.0	41.0	34.0	41.0
22-23	38.12668869226151	40.0	39.0	41.0	33.0	41.0
24-25	38.116279726140114	40.0	38.5	41.0	33.0	41.0
26-27	38.07567295302545	40.0	38.0	41.0	33.0	41.0
28-29	38.043562968342414	40.0	38.0	41.0	33.0	41.0
30-31	38.04963167647452	40.0	38.0	41.0	33.0	41.0
32-33	38.10876562226919	40.5	39.0	41.0	33.0	41.0
34-35	38.07824486729824	41.0	38.0	41.0	33.0	41.0
36-37	37.99758576032809	40.5	38.0	41.0	33.0	41.0
38-39	37.911738860465846	40.0	38.0	41.0	33.0	41.0
40-41	37.8399868758284	40.0	38.0	41.0	33.0	41.0
42-43	37.72254209450489	40.0	38.0	41.0	32.0	41.0
44-45	37.59182008052531	40.0	38.0	41.0	32.0	41.0
46-47	37.457183068624545	40.0	38.0	41.0	31.5	41.0
48-49	37.33818433762013	40.0	37.5	41.0	31.0	41.0
50-51	37.20729080841206	40.0	37.0	41.0	31.0	41.0
52-53	37.05332929512669	40.0	37.0	41.0	31.0	41.0
54-55	36.89556649417445	40.0	36.5	41.0	30.5	41.0
56-57	36.73291035289801	40.0	36.0	41.0	30.0	41.0
58-59	36.531635875340996	39.5	35.5	41.0	30.0	41.0
60-61	36.30291679014678	39.0	35.0	41.0	30.0	41.0
62-63	36.03907100621906	39.0	35.0	41.0	30.0	41.0
64-65	35.77169749055852	38.5	35.0	41.0	29.5	41.0
66-67	35.48097347918641	38.0	35.0	40.0	29.0	41.0
68-69	35.175239442857745	37.0	35.0	40.0	29.0	41.0
70-71	34.829269887896345	37.0	34.0	40.0	29.0	41.0
72-73	34.47399373209539	36.0	34.0	39.0	28.5	41.0
74-75	34.110325813801765	36.0	34.0	39.0	28.0	41.0
76-77	33.78409570766858	35.0	34.0	38.5	28.0	40.0
78-79	33.41980686082624	35.0	34.0	37.0	27.0	39.5
80-81	33.064498410227515	35.0	34.0	37.0	27.0	39.0
82-83	32.84477274774994	35.0	34.0	36.5	27.0	39.0
84-85	32.57169010889046	35.0	33.5	36.0	27.0	37.5
86-87	32.31350025673007	35.0	33.0	36.0	26.0	37.0
88-89	31.95305259112832	35.0	33.0	35.0	25.5	37.0
90-91	31.756436977380616	35.0	33.0	35.0	25.0	36.0
92-93	31.572241834301092	35.0	33.0	35.0	25.0	36.0
94-95	31.39334738021344	35.0	33.0	35.0	25.0	36.0
96-97	31.21076616286782	35.0	33.0	35.0	24.0	36.0
98-99	31.00644240507772	35.0	33.0	35.0	23.0	35.0
100	30.838042403340857	35.0	33.0	35.0	20.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.06908278686653802
1101	2	0.027363830434488534
1101	3	0.06649508238445989
1101	4	0.054644414968514354
1101	5	0.02724485390407949
1101	6	0.036399520015656606
1101	7	0.10729825158115602
1101	8	0.034290828339379686
1101	9	0.033180299233343646
1101	10-11	0.05078706855931614
1101	12-13	0.09944588320126968
1101	14-15	0.06459633087911953
1101	16-17	0.011454384549800523
1101	18-19	0.08840964730776335
1101	20-21	0.060008328636719455
1101	22-23	0.07993606865787228
1101	24-25	0.15702329455864117
1101	26-27	0.044353528897104866
1101	28-29	0.15358692933667584
1101	30-31	0.06874182463686651
1101	32-33	0.0761055230824752
1101	34-35	0.07208377372446506
1101	36-37	0.013352150293002296
1101	38-39	0.07385274182696122
1101	40-41	0.145367178310849
1101	42-43	0.05213207926547625
1101	44-45	-0.03307732618134196
1101	46-47	0.05514859407735884
1101	48-49	0.023320286613206065
1101	50-51	0.03369055090218609
1101	52-53	-0.002868758459122489
1101	54-55	-0.044940220277418064
1101	56-57	8.421675285106289E-4
1101	58-59	0.020258419280061446
1101	60-61	-0.06628957071390573
1101	62-63	0.04219010278156787
1101	64-65	-0.022085255604267218
1101	66-67	0.020679961892781762
1101	68-69	0.04357859174226064
1101	70-71	0.03141332219002635
1101	72-73	0.12954348727102882
1101	74-75	0.001149117944017064
1101	76-77	-0.0721476420385514
1101	78-79	-0.04119858356430228
1101	80-81	0.009045621034267981
1101	82-83	0.03130987047176603
1101	84-85	-0.14290522943419148
1101	86-87	-0.046442404043069985
1101	88-89	0.12448693303052139
1101	90-91	0.16420856612569779
1101	92-93	0.162789828507659
1101	94-95	0.16352793485139827
1101	96-97	0.1648907190425959
1101	98-99	0.2761829419793891
1101	100	0.34555098901147474
1102	1	0.05895986793857233
1102	2	0.08740812171775048
1102	3	0.02362459254484861
1102	4	-0.024349513403507217
1102	5	-0.07763905322162401
1102	6	-0.06176200452378566
1102	7	-0.17689759183303977
1102	8	-0.016691611278460528
1102	9	-0.02307906082881317
1102	10-11	-0.09899594037513282
1102	12-13	0.007176748080375717
1102	14-15	0.08957747023678309
1102	16-17	0.05501591083791624
1102	18-19	0.035103507856426575
1102	20-21	0.019998785901130134
1102	22-23	-0.00139217646552936
1102	24-25	0.00868777747102456
1102	26-27	-0.054615505925134755
1102	28-29	-0.06312903313688167
1102	30-31	-0.04150674863673487
1102	32-33	-0.12565432473337523
1102	34-35	-0.0742237559185881
1102	36-37	-0.08198761605986249
1102	38-39	-0.12499619691841701
1102	40-41	-0.08221925901373339
1102	42-43	-0.10017494576172226
1102	44-45	-0.1437992890870774
1102	46-47	-0.09984060789264504
1102	48-49	-0.1869899272364961
1102	50-51	-0.13902656194566276
1102	52-53	-0.14970401137284028
1102	54-55	-0.14555222314023553
1102	56-57	-0.17566768309769287
1102	58-59	-0.2373773305054172
1102	60-61	-0.2457907379503439
1102	62-63	-0.24175187139059062
1102	64-65	-0.178479169487332
1102	66-67	-0.26346545116152953
1102	68-69	-0.1774481516493296
1102	70-71	-0.08033707656891664
1102	72-73	-0.06711539990110538
1102	74-75	-0.05253891828841262
1102	76-77	-0.18104849126707734
1102	78-79	-0.1498605521407228
1102	80-81	-0.14960938409308255
1102	82-83	-0.06598934175293181
1102	84-85	-0.127121619707097
1102	86-87	-0.2272300919156791
1102	88-89	-0.057709682296067655
1102	90-91	0.0026115818299956572
1102	92-93	0.033958214074143456
1102	94-95	0.08083479125696158
1102	96-97	0.09234125981842567
1102	98-99	0.15616321324788984
1102	100	0.09945301859145417
1103	1	0.07653859861073542
1103	2	0.07360908351769169
1103	3	0.20483633642744792
1103	4	0.18639905415387403
1103	5	0.15363005992822565
1103	6	0.19715289335653097
1103	7	0.21858299803275116
1103	8	0.149891565810087
1103	9	0.1948579594042883
1103	10-11	0.2687282410691836
1103	12-13	0.25158533176317377
1103	14-15	0.28495411557782546
1103	16-17	0.20938336993994966
1103	18-19	0.2511617144048657
1103	20-21	0.38356518635531245
1103	22-23	0.3047876624711421
1103	24-25	0.2598566315646309
1103	26-27	0.31910054363427776
1103	28-29	0.24153725901938827
1103	30-31	0.2292463750661966
1103	32-33	0.2291969564738423
1103	34-35	0.24845392183072335
1103	36-37	0.2867846577668729
1103	38-39	0.22425502083670779
1103	40-41	0.21704883556326138
1103	42-43	0.1957019810584697
1103	44-45	0.21872885882783066
1103	46-47	0.20846910981717315
1103	48-49	0.1773156541610632
1103	50-51	0.14888545912234008
1103	52-53	0.10575590885882491
1103	54-55	0.1522011319480967
1103	56-57	0.14397991932016652
1103	58-59	0.23222202686337567
1103	60-61	0.15295310171149623
1103	62-63	0.22708832717147942
1103	64-65	0.18211679104834388
1103	66-67	0.12492329539750813
1103	68-69	0.12270075797678714
1103	70-71	0.16766513709760744
1103	72-73	0.11625961858586464
1103	74-75	0.09996165421156178
1103	76-77	0.04798041759570282
1103	78-79	0.07496942729698475
1103	80-81	0.07220363980402311
1103	82-83	0.01641278460735407
1103	84-85	0.007430482560479845
1103	86-87	-0.0017447192897677155
1103	88-89	0.09038472771886674
1103	90-91	0.13448187957768454
1103	92-93	0.13492365177422272
1103	94-95	0.07564233585232216
1103	96-97	0.028637220831178922
1103	98-99	0.03933505388298286
1103	100	0.12825513397882204
1104	1	0.03631443381932087
1104	2	-0.05483170772004442
1104	3	-0.07765219992529637
1104	4	-0.08186938356568874
1104	5	-0.2726076066807437
1104	6	-0.23348949515504813
1104	7	-0.20319861057745214
1104	8	-0.1954841125900586
1104	9	-0.16177729466271273
1104	10-11	-0.2401828142651823
1104	12-13	-0.1522413711527122
1104	14-15	-0.29621440427948187
1104	16-17	-0.21611649938635225
1104	18-19	-0.19223684546913944
1104	20-21	-0.14857481608845546
1104	22-23	-0.10854279434374092
1104	24-25	-0.17569408171011958
1104	26-27	-0.1018342315221119
1104	28-29	-0.024336837719879156
1104	30-31	-0.04424349517651649
1104	32-33	-0.04884549211369915
1104	34-35	-0.038708744863093614
1104	36-37	0.02983222299340582
1104	38-39	-0.050775780820977445
1104	40-41	-0.03180119345648791
1104	42-43	-0.009780714010211966
1104	44-45	-0.06708655354103144
1104	46-47	-0.19036102116200482
1104	48-49	-0.16910201971131755
1104	50-51	-0.14280284507245256
1104	52-53	-0.19849340467998644
1104	54-55	-0.23796189621972275
1104	56-57	-0.18441836053877836
1104	58-59	-0.14976031861073835
1104	60-61	-0.19438183364744788
1104	62-63	-0.17884421634965264
1104	64-65	-0.1641763609709983
1104	66-67	-0.22055038827710405
1104	68-69	-0.1714018276653988
1104	70-71	-0.1402771933744802
1104	72-73	-0.16616803570243377
1104	74-75	-0.25120800267664123
1104	76-77	-0.2652718047057192
1104	78-79	-0.16805204561622133
1104	80-81	-0.16012804568305228
1104	82-83	-0.21893627539054705
1104	84-85	-0.1356956141652077
1104	86-87	-0.20825265135301407
1104	88-89	-0.08213637523503792
1104	90-91	-0.11860910495530419
1104	92-93	-0.11342107732392392
1104	94-95	-0.06580854950441761
1104	96-97	-0.10003627990025166
1104	98-99	-0.041658456555641266
1104	100	-0.09183628669884669
1105	1	0.03342618114320217
1105	2	0.06150595217575727
1105	3	0.024486191908277277
1105	4	0.06023367613979502
1105	5	0.04594412776557988
1105	6	0.04256196368174159
1105	7	-0.051361766012441024
1105	8	-0.005310285824350558
1105	9	-0.023437133652642217
1105	10-11	0.039819025386208295
1105	12-13	0.047415520336933525
1105	14-15	0.09136348500461366
1105	16-17	0.07083029478439329
1105	18-19	0.061278545717172506
1105	20-21	0.11087798231212531
1105	22-23	0.158080924579167
1105	24-25	0.10263989753022429
1105	26-27	0.1324161545003264
1105	28-29	0.10530508631198643
1105	30-31	0.1324112611001098
1105	32-33	0.13964769136920552
1105	34-35	0.04470355144792393
1105	36-37	0.07809967042976496
1105	38-39	0.1038087724650012
1105	40-41	0.1360685511092754
1105	42-43	0.04533131873017027
1105	44-45	0.078774129388961
1105	46-47	0.1296210422369839
1105	48-49	0.13990503521647213
1105	50-51	0.10991811430574217
1105	52-53	0.11441790450927414
1105	54-55	0.16570354153276412
1105	56-57	0.14770722813275228
1105	58-59	0.07672897049251048
1105	60-61	0.016658103696187254
1105	62-63	0.036462156622086184
1105	64-65	0.013804296988439546
1105	66-67	0.04167284394979731
1105	68-69	0.0297794720077178
1105	70-71	0.04330107736674904
1105	72-73	0.034995746701973474
1105	74-75	0.08739917832138389
1105	76-77	0.025774571417770176
1105	78-79	0.04743971281320114
1105	80-81	-0.06302556425033856
1105	82-83	0.030652745137125237
1105	84-85	0.07305064312491538
1105	86-87	0.12251835871705197
1105	88-89	0.035821192394131174
1105	90-91	0.039728945866418286
1105	92-93	0.1508965844791632
1105	94-95	0.10123788343183548
1105	96-97	0.14962849097860698
1105	98-99	0.13981571291277461
1105	100	0.11700486143299216
1106	1	0.13206497448310728
1106	2	0.12032118267665837
1106	3	0.10081744336010345
1106	4	0.12304026589189476
1106	5	0.06988427771879202
1106	6	0.09141697259940429
1106	7	0.05598483904208251
1106	8	0.005918094258362316
1106	9	0.1976078929953573
1106	10-11	0.14121993171990255
1106	12-13	0.14738861642589285
1106	14-15	0.1955425810594491
1106	16-17	0.054582497952232245
1106	18-19	0.0810353343613599
1106	20-21	0.08113492122450339
1106	22-23	0.012154636045877965
1106	24-25	0.01645197174302382
1106	26-27	0.029626743879710205
1106	28-29	0.06166084000864913
1106	30-31	-0.002034538018222065
1106	32-33	0.04461730307359346
1106	34-35	-0.026880537710319174
1106	36-37	-0.02421085057431327
1106	38-39	-0.06085444962613451
1106	40-41	-0.06063092957962368
1106	42-43	-0.01292470413684299
1106	44-45	-0.008825333057771445
1106	46-47	-0.06629730636158371
1106	48-49	-0.038671446040680735
1106	50-51	-0.11279318310583619
1106	52-53	-0.14376180311739262
1106	54-55	-0.03484168919124642
1106	56-57	-0.05356690172258283
1106	58-59	-0.06550308295834384
1106	60-61	-0.06311028643939665
1106	62-63	0.02069279007138647
1106	64-65	-0.05382550782040596
1106	66-67	0.032040521984789905
1106	68-69	-0.018279509239597758
1106	70-71	-0.07525133473292556
1106	72-73	-0.021575828856676083
1106	74-75	0.022196106288454587
1106	76-77	0.022786596809808657
1106	78-79	-0.01183898676985251
1106	80-81	-0.03870902201973081
1106	82-83	-0.0341736721412218
1106	84-85	-0.06973025011117784
1106	86-87	-0.08832070208710263
1106	88-89	-0.06430774952098162
1106	90-91	-0.06998395659311996
1106	92-93	-0.01358113186437393
1106	94-95	-0.0885183540102723
1106	96-97	-0.03747849865748165
1106	98-99	-0.057647510753216835
1106	100	-0.03432793207226936
1107	1	0.1208627216800835
1107	2	0.11103803734544115
1107	3	0.03194260962606066
1107	4	0.006345284035695897
1107	5	-0.03310752489957025
1107	6	0.05739130453696362
1107	7	-0.045061898027661584
1107	8	0.0033607761394449653
1107	9	-0.027587374399686837
1107	10-11	0.006028925029880838
1107	12-13	-0.0774906785169378
1107	14-15	-0.011806508528131587
1107	16-17	0.008980870291303233
1107	18-19	0.0450484888405569
1107	20-21	0.002016940870298356
1107	22-23	-0.0034211051467281095
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2215	98-99	-0.065699360595314
2215	100	-0.0938130061117377
2216	1	0.057438009172095406
2216	2	0.07985492436356978
2216	3	0.13515261225212427
2216	4	0.11817447025887162
2216	5	0.21746324064074685
2216	6	0.2419288535879076
2216	7	0.23041869461878406
2216	8	0.17279890696595146
2216	9	0.08084570992519247
2216	10-11	0.16594156041070107
2216	12-13	0.06381291309300252
2216	14-15	0.10974838556689548
2216	16-17	0.080507891459483
2216	18-19	0.058375204378343426
2216	20-21	0.057568434998309215
2216	22-23	0.027029515268850446
2216	24-25	0.06970768881923561
2216	26-27	-0.038744071315740314
2216	28-29	0.043335554026889156
2216	30-31	-0.01935444282968035
2216	32-33	0.027652997888111486
2216	34-35	0.11896393129131155
2216	36-37	0.09270999490718879
2216	38-39	0.05516538028242479
2216	40-41	0.1302437342363092
2216	42-43	0.0633896904381075
2216	44-45	0.06259077680694247
2216	46-47	0.1440211800568676
2216	48-49	0.18225450156111123
2216	50-51	0.18751937961922494
2216	52-53	0.23273820200074624
2216	54-55	0.21301803721512158
2216	56-57	0.16955717673364745
2216	58-59	0.17185421544581914
2216	60-61	0.2019737690341472
2216	62-63	0.1810908079706408
2216	64-65	0.13805475969377312
2216	66-67	0.06639961262887084
2216	68-69	0.06845342030939605
2216	70-71	0.028306931311668393
2216	72-73	0.14527783544392747
2216	74-75	0.1877877034559461
2216	76-77	0.06677825379280478
2216	78-79	0.16650980261146486
2216	80-81	0.2642159072942718
2216	82-83	0.2315769657350799
2216	84-85	0.2293287015419594
2216	86-87	0.2475944373333121
2216	88-89	0.2401628587680058
2216	90-91	0.2261971526551818
2216	92-93	0.27297853744132894
2216	94-95	0.2835313151129526
2216	96-97	0.3120039458918953
2216	98-99	0.29754411026926064
2216	100	0.3122866838289511
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	24123.0
3	4076.0
4	2588.0
5	2134.0
6	1810.0
7	1863.0
8	2264.0
9	2459.0
10	2670.0
11	2877.0
12	3394.0
13	3651.0
14	3980.0
15	4402.0
16	4233.0
17	4312.0
18	4113.0
19	4205.0
20	4194.0
21	4339.0
22	5096.0
23	6847.0
24	10331.0
25	14226.0
26	14729.0
27	13014.0
28	13574.0
29	16972.0
30	20658.0
31	25061.0
32	33117.0
33	44919.0
34	63034.0
35	96634.0
36	182604.0
37	369248.0
38	576644.0
39	247450.0
40	557.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.07230113009825	19.797221612632182	13.322014017727179	23.808463239542384
2	15.500839274002177	19.911252683693416	40.746277334820064	23.841630707484345
3	18.38057783978923	25.278494837981203	29.629482787930073	26.711444534299496
4	12.224278929298448	16.61056725115398	39.02369098498048	32.141462834567086
5	12.820561551510307	38.116226436404624	35.626668375314665	13.436543636770395
6	31.135270460039095	38.10829692331994	17.749413341454	13.007019275186963
7	27.452486425662354	32.55908629766759	22.33530174573337	17.65312553093668
8	24.91615665812219	37.10017664372578	20.19525265915578	17.788414038996248
9	24.779650785309617	17.345701263140064	21.133438576909967	36.74120937464035
10-11	23.882067256744786	27.1658606657054	28.730287901950906	20.22178417559891
12-13	24.440213787508288	24.459702314113247	28.786128077619704	22.313955820758753
14-15	21.770485840619088	26.516545290204203	25.733755323509218	25.979213545667495
16-17	21.53143633297386	29.845386807133472	26.28798861702067	22.335188242871997
18-19	21.397140953205604	28.881638502807338	28.245254261901497	21.475966282085558
20-21	22.592645877217745	28.256069621490155	27.67446090034528	21.47682360094682
22-23	22.214218128855286	27.989868156839453	27.779104756331453	22.016808957973804
24-25	22.046872395744263	27.99161969166155	27.800326504109535	22.161181408484655
26-27	21.833253938202088	27.928845676222586	27.907537923050512	22.330362462524818
28-29	21.89498096159421	28.04904927402167	27.902664725368147	22.153305039015976
30-31	22.106779498153948	28.51657863921448	27.495671915741198	21.880969946890374
32-33	21.733677787969295	28.117281428793632	28.06950585671509	22.079534926521983
34-35	21.907748363370665	27.817500934383098	28.075871093403297	22.19887960884294
36-37	22.18451422775647	28.233770385997598	27.55353372671601	22.02818165952992
38-39	22.00306051935461	28.441577899120112	27.7010657533964	21.854295828128876
40-41	21.82681075400496	28.114048515483525	27.987244709632314	22.071896020879205
42-43	21.770394469539283	27.966595982539673	27.997943800283732	22.265065747637312
44-45	21.92127224623308	28.117648144469015	27.962183710757987	21.998895898539917
46-47	21.889169975793234	28.402470126939644	27.777203300197286	21.93115659706984
48-49	21.858276016578007	28.16304220682558	27.82227630860103	22.156405467995388
50-51	21.88626746716091	28.00947305198427	27.899799080323636	22.20446040053119
52-53	21.91723826844625	28.03498086186864	27.873386259139227	22.174394610545882
54-55	22.005725324464905	28.43428550957722	27.664069789468943	21.895919376488933
56-57	21.824085846996834	28.15891138886665	27.760343800504515	22.256658963632
58-59	21.73043051111491	27.80046625360614	28.106435005387155	22.362668229891796
60-61	22.047379365485167	28.209687671149595	27.692785350652795	22.050147612712443
62-63	21.866023643844244	28.224866202763877	27.752993475688527	22.156116677703356
64-65	21.869275298600424	27.871815539434795	27.925768517840034	22.333140644124743
66-67	21.68454905047188	28.055336558359173	27.757783823758896	22.502330567410052
68-69	22.17008719657673	28.16271121673814	27.62220045632396	22.045001130361175
70-71	22.119822922004047	27.923219884148086	27.790563366826753	22.16639382702111
72-73	21.850747094977418	28.04738680270163	27.936496994766795	22.16536910755416
74-75	21.94384293325293	28.086648007531682	27.769145990407363	22.200363068808027
76-77	21.876901465482007	28.101739700129563	27.654859691184363	22.366499143204063
78-79	22.044477590633885	28.13897082710146	27.738963987985954	22.0775875942787
80-81	21.921268698134615	27.931594212653053	27.914306317083888	22.23283077212844
82-83	21.785794443149303	28.067043484797864	27.989778308378426	22.157383763674403
84-85	21.852436152580832	27.969488827125698	27.776419301199045	22.401655719094425
86-87	22.000296359500357	28.075584699382368	27.728230739210698	22.19588820190658
88-89	21.798155569873217	27.973502014786018	27.798395815360422	22.429946599980344
90-91	21.781902207582608	27.780897979065195	27.94745028891365	22.489749524438547
92-93	21.94160830263602	28.019021231656694	27.73477302189347	22.304597443813815
94-95	21.700731341776024	27.855686257380984	27.94266105762012	22.50092134322287
96-97	21.73975355406766	27.81390135447261	27.954625426915342	22.491719664544384
98-99	21.722801661447345	27.915476486851244	27.83598557807798	22.52573627362343
100	21.85336419161618	27.82519536208526	27.76737702957907	22.55406341671949
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	455.0
1	426.5
2	370.0
3	295.5
4	221.5
5	179.5
6	158.0
7	147.0
8	159.0
9	176.0
10	171.0
11	175.0
12	174.5
13	188.5
14	225.5
15	265.5
16	348.5
17	454.0
18	650.0
19	939.5
20	1354.5
21	2072.0
22	3081.0
23	4595.5
24	6625.5
25	9020.5
26	12160.0
27	15981.0
28	20685.0
29	25858.5
30	30794.5
31	36032.5
32	41219.0
33	46373.5
34	51202.0
35	54897.0
36	57614.5
37	60587.5
38	62985.5
39	63841.0
40	64966.0
41	65817.0
42	65313.5
43	64064.0
44	62585.0
45	61288.5
46	60659.5
47	59529.0
48	58019.5
49	56692.0
50	55219.0
51	53424.5
52	50435.0
53	47832.0
54	45860.0
55	44075.5
56	41583.5
57	38139.5
58	34873.0
59	31915.0
60	28910.5
61	25182.5
62	21728.0
63	18525.5
64	15371.0
65	12631.5
66	10251.0
67	8026.0
68	6116.0
69	4772.0
70	3651.0
71	2763.0
72	2174.0
73	1769.0
74	1412.5
75	1069.0
76	804.5
77	589.5
78	441.0
79	320.0
80	213.0
81	129.0
82	82.0
83	60.0
84	48.0
85	36.0
86	22.0
87	16.5
88	13.0
89	9.5
90	7.0
91	7.0
92	3.5
93	3.0
94	4.5
95	3.5
96	3.0
97	2.0
98	2.0
99	2.0
100	2.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.32636742686992304
2	0.1151214555780986
3	0.06323267126284057
4	0.03967646583101842
5	0.04287880712244125
6	0.01025834752676126
7	0.007544498974708017
8	0.014980444007333903
9	0.017694292559387148
10-11	0.020950910821851042
12-13	0.015876014029511474
14-15	0.018291339240838863
16-17	0.01918690926301643
18-19	0.019241186234057496
20-21	0.02399042120015067
22-23	0.015686044630867747
24-25	0.025238791534095167
26-27	0.018969801378852173
28-29	0.018237062269797797
30-31	0.019702540487906546
32-33	0.024777437280246114
34-35	0.0150618594638955
36-37	0.009525608417706884
38-39	0.012537980310485985
40-41	0.010095516613638066
42-43	0.009145669620419431
44-45	0.009769854787391676
46-47	0.008060130199598134
48-49	0.004423573139846787
50-51	0.006703205923571512
52-53	0.0074630835181464196
54-55	0.0033108952335049575
56-57	0.0029580949217380354
58-59	0.0030937873493406976
60-61	0.004396434654326255
62-63	0.003365172204546022
64-65	0.0030937873493406976
66-67	0.0021439403561220624
68-69	0.003826526458395074
70-71	0.0027138485520532434
72-73	0.0017640015588346082
74-75	0.0027952640086148406
76-77	0.003962218885997735
78-79	0.003175202805902295
80-81	0.003935080400477203
82-83	0.002469602182368452
84-85	0.003283756747984425
86-87	0.002442463696847919
88-89	0.5938986171313319
90-91	0.6049439807381886
92-93	0.6013888391349987
94-95	0.58819953517202
96-97	0.5863812566421444
98-99	0.5832603308072831
100	0.5863541181566237
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1842402.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	74.49990657486902
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.41368545142997	62.14311773197042
2	11.022171160855192	16.423014434718546
3	2.82495653959278	6.31376994833182
4	1.0557564130590338	3.146150165548673
5	0.5309636328850538	1.9778370522294773
6	0.2995833765537368	1.3391360138782313
7	0.18663280775930735	0.9732888719311719
8	0.1271136978797556	0.7575966893142334
9	0.0947373984091025	0.6352134597561853
>10	0.4282092239674731	5.384923025070068
>50	0.014514066616046487	0.712753792228279
>100	0.001603407138378461	0.15744562455838634
>500	7.282385412133193E-5	0.03575319046455877
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	5.427697104106487E-5	0.0
12-13	0.0	0.0	0.0	1.0855394208212974E-4	0.0
14-15	0.0	0.0	0.0	1.0855394208212974E-4	0.0
16-17	0.0	0.0	0.0	1.0855394208212974E-4	0.0
18-19	0.0	0.0	0.0	1.0855394208212974E-4	0.0
20-21	0.0	0.0	0.0	2.171078841642595E-4	0.0
22-23	0.0	0.0	0.0	2.4424636968479194E-4	0.0
24-25	0.0	5.427697104106487E-5	0.0	2.7138485520532437E-4	0.0
26-27	0.0	5.427697104106487E-5	0.0	5.699081959311812E-4	0.0
28-29	0.0	8.14154565615973E-5	0.0	0.0013840627615471543	0.0
30-31	0.0	1.0855394208212974E-4	0.0	0.003989357371518268	0.0
32-33	0.0	1.0855394208212974E-4	0.0	0.009308500533542626	0.0
34-35	0.0	1.0855394208212974E-4	0.0	0.017857123472510342	0.0
36-37	0.0	1.3569242760266218E-4	0.0	0.03509006177804844	0.0
38-39	0.0	1.628309131231946E-4	0.0	0.06331408671940217	0.0
40-41	0.0	1.628309131231946E-4	0.0	0.1018235976730377	0.0
42-43	0.0	1.628309131231946E-4	0.0	0.1394104001189751	0.0
44-45	0.0	1.628309131231946E-4	0.0	0.17889689655134983	0.0
46-47	0.0	1.628309131231946E-4	0.0	0.22837035565528044	0.0
48-49	0.0	1.628309131231946E-4	0.0	0.28587680647328867	0.0
50-51	0.0	1.628309131231946E-4	0.0	0.3497879398741426	0.0
52-53	0.0	1.628309131231946E-4	0.0	0.41540879786279	0.0
54-55	0.0	1.628309131231946E-4	0.0	0.4886284317971865	0.0
56-57	0.0	1.8996939864372703E-4	0.0	0.5684969946841134	0.0
58-59	0.0	2.171078841642595E-4	0.0	0.6622333236720325	0.0
60-61	0.0	2.171078841642595E-4	0.0	0.76557667653422	0.0
62-63	0.0	2.171078841642595E-4	0.0	0.8684858136280791	0.0
64-65	2.7138485520532436E-5	2.171078841642595E-4	0.0	0.9778810487613452	0.0
66-67	5.427697104106487E-5	3.7993879728745407E-4	0.0	1.10146971182185	0.0
68-69	5.427697104106487E-5	3.7993879728745407E-4	0.0	1.2439738992901659	0.0
70-71	5.427697104106487E-5	3.7993879728745407E-4	0.0	1.4004544068015559	0.0
72-73	5.427697104106487E-5	3.7993879728745407E-4	0.0	1.5670847078976249	0.0
74-75	5.427697104106487E-5	3.7993879728745407E-4	0.0	1.7360489187484598	0.0
76-77	8.14154565615973E-5	3.7993879728745407E-4	0.0	1.9121234128056743	0.0
78-79	1.0855394208212974E-4	4.34215768328519E-4	0.0	2.121333997683459	0.0
80-81	1.0855394208212974E-4	4.34215768328519E-4	0.0	2.350491369418835	0.0
82-83	1.0855394208212974E-4	4.34215768328519E-4	0.0	2.5922952754067787	0.0
84-85	1.0855394208212974E-4	4.34215768328519E-4	0.0	2.8234337565851537	0.0
86-87	1.0855394208212974E-4	4.34215768328519E-4	0.0	3.071370960300738	0.0
88	1.0855394208212974E-4	4.34215768328519E-4	0.0	3.278763266648647	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	2405	0.0	32.709995	1
TCAACGC	2295	0.0	32.340668	4
GGTATCA	1510	0.0	32.132122	1
ATCAACG	2375	0.0	31.253849	3
CAACGCA	2425	0.0	30.606945	5
AACGCAG	2595	0.0	28.775074	6
TATCAAC	2870	0.0	26.53111	2
CGCGTAT	45	9.5654256E-4	26.09061	12-13
GTACATG	5080	0.0	26.056837	1
TACATGG	5100	0.0	25.068079	2
CCGGTCG	155	1.5582282E-6	24.239017	9
ACGCAGA	3065	0.0	23.902914	7
ACATGGG	5285	0.0	23.289831	3
CGCAGAG	3145	0.0	23.145563	8
GTATAGA	845	0.0	20.626432	1
CATGGGG	3680	0.0	19.147762	4
GCAGAGT	3840	0.0	18.22266	9
TATAACG	155	0.0010332549	18.194574	2
GTACCGT	155	0.0010391109	18.179262	6
GTATTAG	960	0.0	17.174177	1
>>END_MODULE