FastQCFastQC Report
Wed 25 May 2016
SRR1033108_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033108_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1842402
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36810.1997935304021598No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30090.16331940586256422No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26550.052.4611361
GTATCAA48350.041.8487551
ATCAACG57500.034.260293
TCAACGC58100.033.9874084
CAACGCA58700.033.72015
AACGCAG59800.033.0059746
TATCAAC61200.032.4194682
ACGCAGA69950.028.0823217
GTACATG51850.027.6797161
CGCAGAG71150.027.4765938
CTAGCGT700.00882280626.8662344
TACATGG51550.026.7229142
ACATGGG51200.025.5281723
GCAGAGT79600.024.5007559
TCTAGCG1000.001755619523.5079563
TAGATCG1000.001755619523.5079565
GAGTACT53250.022.06301512-13
AGAGTAC72100.020.9551210-11
GTCTCGC4500.020.9135631
CAGAGTA78650.020.79337310-11
Produced by FastQC (version 0.11.5)